Analysis of physiological and miRNA responses to Pi deficiency in alfalfa (Medicago sativa L.).

abstract：:Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcriptio...

journal_title：Plant molecular biology

authors： Ogo Y,Itai RN,Kobayashi T,Aung MS,Nakanishi H,Nishizawa NK

更新日期：2011-04-01 00:00:00

abstract：:The expression of the mat-r locus (mat-r-nad1e-nad5III) was studied in wheat mitochondria. Transcription initiation sites were mapped by S1 protection, primer extension and capping experiments. Two different transcription initiation sites were found. One, non-canonical promoter of low expression level generates a tran...

journal_title：Plant molecular biology

authors： Farré JC,Araya A

更新日期：1999-08-01 00:00:00

abstract：:The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNA(Arg)(ACG) and tRNA(Asn)(GUU). The tRNA(Arg)(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation a...

journal_title：Plant molecular biology

authors： Dormann-Przybyl D,Strittmatter G,Kössel H

更新日期：1986-11-01 00:00:00

abstract：:In this report, we describe the efficient expression and glycosylation, in insect cells, of β-phaseolin polypeptides (M r 45 and 48 kDa) from Phaseolus vulgaris, by means of a baculovirus expression vector. N-terminal sequence analysis demonstrated that the signal peptide was efficiently processed. Tunicamycin treatme...

journal_title：Plant molecular biology

authors： Bustos MM,Luckow VA,Griffing LR,Summers MD,Hall TC

更新日期：1988-11-01 00:00:00

abstract：:A number of A/T-rich sequences and a CATGCAT/A sequence are contained in the 5'-upstream regions of the genes encoding concanavalin A (Con A) and canavalin, two major seed storage proteins of Canavalia gladiata D.C. To study the role of these sequences in the seed-specific gene expression, we constructed 5'-deletion m...

journal_title：Plant molecular biology

authors： Yamamoto S,Nishihara M,Morikawa H,Yamauchi D,Minamikawa T

更新日期：1995-02-01 00:00:00

abstract：KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...

journal_title：Plant molecular biology

authors： Zhang L,Wang Y,Zhang Q,Jiang Y,Zhang H,Li R

更新日期：2020-01-01 00:00:00

abstract：KEY MESSAGE:The design and use of existing VIGS vectors for revealing monocot gene functions are described and potential new vectors are discussed, which may expand their repertoire. Virus induced gene silencing (VIGS) is a method of transient gene silencing in plants, triggered by the use of modified viral vectors. VI...

journal_title：Plant molecular biology

authors： Kant R,Dasgupta I

更新日期：2019-05-01 00:00:00

abstract：KEY MESSAGE:Overexpression of BoMYB29 gene up-regulates the aliphatic glucosinolate pathway in Brassica oleracea plants increasing the production of the anti-cancer metabolite glucoraphanin, and the toxic and pungent sinigrin. Isothiocyanates, the bio-active hydrolysis products of glucosinolates, naturally produced by ...

journal_title：Plant molecular biology

authors： Zuluaga DL,Graham NS,Klinder A,van Ommen Kloeke AEE,Marcotrigiano AR,Wagstaff C,Verkerk R,Sonnante G,Aarts MGM

更新日期：2019-09-01 00:00:00

abstract：:In planta quantitative studies of cell cycle are necessary for examining the role of cell division in the response of plants to environmental conditions and to analyse the behaviour of transformed plants in this context. We present and discuss non-intrusive kinematic methods which allow estimating the duration of cell...

journal_title：Plant molecular biology

authors： Tardieu F,Granier C

更新日期：2000-08-01 00:00:00

abstract：:The intrinsic chlorophyll-protein CP 47 is a component of photosystem II which functions in both light-harvesting and oxygen evolution. The large extrinsic loop E of this protein has been shown to interact with the oxygen-evolving site. Previously, Vermaas and coworkers have produced a number of deletions within loop ...

journal_title：Plant molecular biology

authors： Putnam-Evans C,Wu J,Bricker TM

更新日期：1996-12-01 00:00:00

abstract：:We have previously shown that two CRE elements situated on a 31 bp region of the cauliflower mosaic virus (CaMV) 35S promoter activate gene expression in the yeast Saccharomyces cerevisiae and are regulated by cAMP. Studies with the yeast transcription factors GCN4, SKO1 and YAP1, which bind CRE-like sequences, showed...

journal_title：Plant molecular biology

authors： Rüth J,Schweyen RJ,Hirt H

更新日期：1994-05-01 00:00:00

abstract：:Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...

journal_title：Plant molecular biology

authors： Morris BA,Richardson KA,Andersen MT,Gardner RC

更新日期：1988-11-01 00:00:00

abstract：:Chromosomal non-histone high-mobility-group (HMG) proteins represent essential components of eukaryotic chromatin and have also been isolated from a variety of plants. In maize, studies on structure and function of the two larger of the four major HMG proteins have recently been performed and are now extended by analy...

journal_title：Plant molecular biology

authors： Grasser KD,Hetz W,Feix G

更新日期：1994-06-01 00:00:00

abstract：:A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600 cDNA clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ESTs). Assembly of the resulting EST sequences resulted i...

journal_title：Plant molecular biology

authors： Li YZ,Pan YH,Sun CB,Dong HT,Luo XL,Wang ZQ,Tang JL,Chen B

更新日期：2010-12-01 00:00:00

abstract：:A subset of homeodomain leucine zipper proteins (HDZip) play a role in regulating adaptation responses including developmental adjustment to environmental cues in plants. Here we report the structural and functional characterisation of a dehydration responsive nuclear-targeted HDZip transcriptional regulator, CpHB-7. ...

journal_title：Plant molecular biology

authors： Deng X,Phillips J,Bräutigam A,Engström P,Johannesson H,Ouwerkerk PB,Ruberti I,Salinas J,Vera P,Iannacone R,Meijer AH,Bartels D

更新日期：2006-06-01 00:00:00

abstract：:Herbaspirillum seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize plants, wheat seedlings growing hydroponically in Hoagland's medium were inoculated wi...

journal_title：Plant molecular biology

authors： Pankievicz VC,Camilios-Neto D,Bonato P,Balsanelli E,Tadra-Sfeir MZ,Faoro H,Chubatsu LS,Donatti L,Wajnberg G,Passetti F,Monteiro RA,Pedrosa FO,Souza EM

更新日期：2016-04-01 00:00:00

abstract：:A method (termed co-cultivation) for transforming plant cells in vitro with A. tumefaciens strains, which was originally developed by Marton et al. (1978) Nature 277: 129-131, has been modified by the incorporation of a novel feeder plate culture system and been extended to use with petunia protoplasts. Using efficien...

journal_title：Plant molecular biology

authors： Fraley RT,Horsch RB,Matzke A,Chilton MD,Chilton WS,Sanders PR

更新日期：1984-11-01 00:00:00

abstract：:Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...

journal_title：Plant molecular biology

authors： Ren F,Zhao CZ,Liu CS,Huang KL,Guo QQ,Chang LL,Xiong H,Li XB

更新日期：2014-12-01 00:00:00

abstract：:RCT1 is a TIR-NBS-LRR-type resistance (R) gene in Medicago truncatula that confers resistance to multiple races of Colletotrichum trifolii, a hemi-biotrophic fungal pathogen that causes anthracnose disease in Medicago and other closely related legumes. RCT1 undergoes alternative splicing at both coding and 3'-untransl...

journal_title：Plant molecular biology

authors： Tang F,Yang S,Gao M,Zhu H

更新日期：2013-07-01 00:00:00

abstract：:cDNA clones for all enzymes of the prechorismate pathway of higher plants have previously been cloned, with the exception of the second enzyme of the pathway, 3-dehydroquinate synthase. Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-d...

journal_title：Plant molecular biology

authors： Bischoff M,Rösler J,Raesecke HR,Görlach J,Amrhein N,Schmid J

更新日期：1996-04-01 00:00:00

abstract：:The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...

journal_title：Plant molecular biology

authors： Abbott AG,O'Dell M,Flavell RB

更新日期：1985-07-01 00:00:00

abstract：:cDNA and genomic clones encoding Brassica napus non-specific lipid transfer proteins (LTP) were isolated and sequenced. The encoded amino acid sequences were very similar to those reported previously for LTPs from B. napus and other species. Sequence information indicates that B. napus contains an LTP gene family. The...

journal_title：Plant molecular biology

authors： Sohal AK,Pallas JA,Jenkins GI

更新日期：1999-09-01 00:00:00

abstract：:Ethylene promotes the abscission of leaves and the ripening of fruits in pepper plants, and in both events an increase in cellulase activity is observed. However, two enzyme isoforms (pI 7.2 and 8.5, respectively) are differentially involved in the two physiological phenomena. The pI 8.5 form has been purified from ri...

journal_title：Plant molecular biology

authors： Ferrarese L,Trainotti L,Moretto P,Polverino de Laureto P,Rascio N,Casadoro G

更新日期：1995-11-01 00:00:00

abstract：KEY MESSAGE:CmCAD2 and CmCAD3 function more positively than CmCAD1 in oriental melon for lignin synthesis which is important to ensure internal water status and thus for drought tolerance. Well-lignification may be the guarantee of efficient axial water transport and barrier of lateral water flow in oriental melon tole...

journal_title：Plant molecular biology

authors： Liu W,Jiang Y,Wang C,Zhao L,Jin Y,Xing Q,Li M,Lv T,Qi H

更新日期：2020-08-01 00:00:00

abstract：:WRKY transcription factors are involved in various biological processes, such as development, metabolism and responses to stress. However, their exact roles in abiotic stress tolerance are largely unknown. Here, we demonstrated a working model for the function of a WRKY gene (ThWRKY4) from Tamarix hispida in the stres...

journal_title：Plant molecular biology

authors： Zheng L,Liu G,Meng X,Liu Y,Ji X,Li Y,Nie X,Wang Y

更新日期：2013-07-01 00:00:00

abstract：:A novel rice genomic sequence encoding coding segments homologous to other metallothionein-like genes was isolated from Oryza sativa genomic library. This sequence, hereby designated as rgMT (rice genomic metallothionein-like gene), consists of two exons and one intron. From the coding sequence, it is predicted that r...

journal_title：Plant molecular biology

authors： Hsieh HM,Liu WK,Huang PC

更新日期：1995-06-01 00:00:00

abstract：:Rhodanese-domain proteins (RDPs) are widespread in plants and other organisms, but their biological roles are mostly unknown. Here we report on a novel RDP from Chlamydomonas that has a single rhodanese domain, and a predicted chloroplast transit peptide. The protein was produced in Escherichia coli with a His-tag, bu...

journal_title：Plant molecular biology

authors： Luo L,Herrin DL

更新日期：2012-07-01 00:00:00

abstract：KEY MESSAGE:Distinct catalytic features of the Poaceae TPS-a subfamily arose early in grass evolution and the reactions catalyzed have become more complex with time. The structural diversity of terpenes found in nature is mainly determined by terpene synthases (TPS). TPS enzymes accept ubiquitous prenyl diphosphates as...

journal_title：Plant molecular biology

authors： Luck K,Chen X,Norris AM,Chen F,Gershenzon J,Köllner TG

更新日期：2020-09-01 00:00:00

abstract：:In many temperate woody species, dormancy is induced by short photoperiods. Earlier studies have shown that the photoreceptor phytochrome A (phyA) promotes growth. Specifically, Populus plants that over-express the oat PHYA gene (oatPHYAox) show daylength-independent growth and do not become dormant. However, we show ...

journal_title：Plant molecular biology

authors： Kozarewa I,Ibáñez C,Johansson M,Ogren E,Mozley D,Nylander E,Chono M,Moritz T,Eriksson ME

更新日期：2010-05-01 00:00:00

abstract：:RbcL and rbcS mRNA levels and plastid transcription activity are low in the basal meristematic region of barley primary leaves and increase coordinately during leaf cell development with a similar time course in dark-grown or illuminated seedlings. The capacity of light to cause cab mRNA accumulation shows a similar d...

journal_title：Plant molecular biology

authors： Rapp JC,Mullet JE

更新日期：1991-10-01 00:00:00