Abstract:
:Synthetic oligonucleotides corresponding to conserved regions of cysteine proteinases were used as primers in the RT-PCR amplification of a fragment of cDNA corresponding to a region of a cysteine proteinase gene expressed during germination of chickpea (cac for Cicer arietinum cysteine proteinase). The identity of the PCR-amplified fragment was confirmed by sequencing and the fragment used as a probe to investigate the pattern of cac gene expression during germination and its hormonal regulation. The corresponding transcript is undetected in the seed during embryogenesis and before imbibition, being detected 24 h after imbibition. Ablation of the embryonic axis before imbibition results in a dramatic decrease in the amount of transcript detected. Expression of the cac transcript in excised cotyledons is restored in the presence of aqueous extracts from embryonic axes and also by incubating the excised cotyledons in 1 mM ethephon. Experiments with various known inhibitors of ethylene action indicate that ethylene activates the expression of cac gene in the cotyledons of chickpea during normal germination.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Cervantes E,Rodríguez A,Nicolás Gdoi
10.1007/BF00023238subject
Has Abstractpub_date
1994-05-01 00:00:00pages
207-15issue
2eissn
0167-4412issn
1573-5028journal_volume
25pub_type
杂志文章abstract::The maize (Zea mays L.) caffeic acid O-methyl-transferase (COMT) is a key enzyme in the biosynthesis of lignin. In this work we have characterized the involvement of COMT in the lignification process through the study of the molecular mechanisms involved in its regulation. The examination of the maize COMT gene promot...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9058-2
更新日期:2006-12-01 00:00:00
abstract:KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00926-7
更新日期:2020-01-01 00:00:00
abstract::Analysis of the structure of chromatin in cereal species using micrococcal nuclease (MNase) cleavage showed nucleosomal organization and a ladder with typical nucleosomal spacing of 175-185 bp. Probing with a set of DNA probes localized in the authentic telomeres, subtelomeric regions and bulk chromatin revealed that ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005912822671
更新日期:1998-01-01 00:00:00
abstract::Calcium-dependent protein kinases (CDPKs) are crucial sensors of calcium concentration changes in plant cells under diverse endogenous and environmental stimuli. We identified 20 CDPK genes from bread wheat and performed a comprehensive study on their structural, functional and evolutionary characteristics. Full-lengt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9281-5
更新日期:2008-03-01 00:00:00
abstract::It has been demonstrated that insertion of foreign DNA into mammalian genome can profoundly alter the patterns of DNA methylation and transcription of the host genome. Introgression of alien DNA into plant genomes through sexual crossing and genetic engineering are commonly used in breeding, but it is not known if pla...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000038270.48326.7a
更新日期:2004-03-01 00:00:00
abstract::Anaerobiosis rapidly induces alcohol dehydrogenase (ADH), an enzyme of the fermentation pathway, in different parts of rice seedlings. After initiation of anaerobiosis, the activity of the enzyme increases linearly for 3 days or more. The ADH activity is anaerobically inducible even in mature rice leaves in contrast t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027335
更新日期:1989-07-01 00:00:00
abstract::The histidine auxotroph mutant his 1(-) isolated from Nicotiana plumbaginifolia haploid protoplasts was first characterized to be deficient for the enzyme histidinol phosphate aminotransferase that is responsible for one of the last steps of histidine biosynthesis. Expression of the mutated gene at the RNA level was a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006493021557
更新日期:2001-01-01 00:00:00
abstract:KEY MESSAGE:This manuscript describes the functions of an Argonaute protein named AGO17 in rice. AGO17 is required for the development of rice reproductive tissues. Argonaute (AGO) proteins are a well-conserved multigene family of regulators mediating gene silencing across eukaryotes. Monocot plants have additional mem...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01071-2
更新日期:2021-01-01 00:00:00
abstract::Tight control of cell/tissue identity is essential for a correct and functional organ patterning, an important component of overall fruit development and eventual maturation and ripening. Despite many investigations regarding the molecular determinants of cell identity in fruits of different species, a useful model ab...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0445-z
更新日期:2016-05-01 00:00:00
abstract::The actin gene superfamily of Petunia hybrida cv. Mitchell contains greater than 100 gene members which have been divided into several highly divergent subfamilies [1]. Five subfamily-specific probes have been used to compare the actin genes among the Mitchell, Violet 23 (V23) and Red 51 (R51) cultivars of P. hybrida....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017466
更新日期:1988-09-01 00:00:00
abstract::Clones encoding beta-1,3-glucanase have been isolated from a Hevea cDNA library prepared from the latex of Hevea brasiliensis using a probe Nicotiana plumbaginifolia cDNA encoding beta-1,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic beta-1,3-glucanase showed 68% nucleo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043663
更新日期:1995-10-01 00:00:00
abstract::This is the first report to describe the detailed analysis of TATA sequence requirements for the initiation of transcription for RNA polymerase II in a plant in vitro transcription system. We examined the transcriptional activities associated with 24 promoters, including wild-type (TATATATA), and the 21 point and two ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006056128129
更新日期:1998-12-01 00:00:00
abstract::ATP-binding cassette transporters are involved in the active transport of a wide variety of metabolites in prokaryotes and eukaryotes. One subfamily, the Pleiotropic Drug Resistance (PDR) transporters, or full-size ABCG transporters, are found only in fungi and plants. NtPDR1 was originally identified in Nicotiana tab...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0053-0
更新日期:2013-05-01 00:00:00
abstract::In higher eukaryotes, the proliferating cell nuclear antigen (PCNA) can be found associated to Cyclin D and Cdk4/6, the kinase complex responsible for cell cycle commitment in response to growth and mitogenic signals. During maize germination, PCNA can be found in protein complexes between 131 and 163 kDa. The sizes o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1016029001537
更新日期:2002-09-01 00:00:00
abstract::The cis-regulatory elements that confer cell cycle-dependent expression to the wheat histone H3 gene were investigated in rice cells (Oc strain) transformed with H3/GUS chimeric genes. 5' deletion mutants of the H3 promoter region (from -1711, -908 or -185 to +57 relative to the transcription start site) were joined t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019303
更新日期:1993-11-01 00:00:00
abstract::We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene cons...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014935
更新日期:1993-05-01 00:00:00
abstract::Cold acclimation in plants is a polygenic phenomenon involving increased expression of several genes. The gene products participate either directly or indirectly towards increasing cold tolerance. Evidence of proteins having a direct effect on cold tolerance is emerging but limited. With isolated protoplasts from warm...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1026599028063
更新日期:2000-11-01 00:00:00
abstract::The nuclear gene mutant of barley, vir-115, shows a developmentally induced loss of D1 synthesis that results in inactivation of Photosystem II. Translation in plastids isolated from 1 h illuminated vir-115 seedlings is similar to wild type. In wild-type barley, illumination of plants for 16 to 72 h results in increas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043874
更新日期:1994-06-01 00:00:00
abstract::The hypersensitive response (HR) involves programmed cell death (PCD) in response to pathogen infection. To investigate the pathogen resistance signaling pathway, we previously identified the Arabidopsis mutant cpr22, which displays constitutive activation of multiple defense responses including HR like cell death. Th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9239-7
更新日期:2007-12-01 00:00:00
abstract::Gene expression for nuclear- and mitochondrial-encoded subunits of respiratory chain components was measured in developing soybean cotyledons and compared to the abundance of the relevant proteins. Overall respiratory gene expression peaked at day 16, close to the peak in cytochrome chain and TCA cycle activities from...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1022502501373
更新日期:2003-03-01 00:00:00
abstract::Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9578-7
更新日期:2010-03-01 00:00:00
abstract::The expression of the mat-r locus (mat-r-nad1e-nad5III) was studied in wheat mitochondria. Transcription initiation sites were mapped by S1 protection, primer extension and capping experiments. Two different transcription initiation sites were found. One, non-canonical promoter of low expression level generates a tran...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006296422485
更新日期:1999-08-01 00:00:00
abstract::Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1997-09-01 00:00:00
abstract::We have analysed the expression of the 8-10 members of the gene family encoding the flavonoid biosynthetic enzyme chalcone synthase (CHS) from Petunia hybrida. During normal plant development only two members of the gene family (CHS-A and CHS-J) are expressed. Their expression is restricted to floral tissues mainly. A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020506
更新日期:1989-02-01 00:00:00
abstract::The molecular mechanism of signal transduction pathways which mediate the action of phytohormones are poorly understood. Recently, we and others have shown that the as -1 type cis-acting elements can respond to auxin and salicylic acid, two well-characterized signaling molecules in plants. In the present work, we have...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019093
更新日期:1996-11-01 00:00:00
abstract::The large number of mitogen-activated protein (MAP) kinase genes identified to date in plants suggests that their encoded proteins have a wide array of functions in development and physiological responses, as has been indicated by studies on the factors which lead to the activation of these kinases. Signalling pathway...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010645431133
更新日期:2001-04-01 00:00:00
abstract::The treatment of barley aleurone layers with gibberellic acid (GA3) results in the synthesis of two groups of α-amylase isozymes. Addition of abscisic acid (ABA) at the same time as GA3 inhibited the synthesis of both groups of isozymes. However, midcourse ABA addition (12 h or later after GA3) had a more inhibitory e...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016430
更新日期:1987-01-01 00:00:00
abstract::The expression of Brazil nut storage albumin genes is highly regulated during seed development. Several sequences in the promoter of one of these genes show homologies with the target sites of the maize O2 bZIP regulatory protein. We therefore asked whether the O2 protein would recognize these promoter sequences. We s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005874404706
更新日期:1997-08-01 00:00:00
abstract::Alpha-dioxygenases (α-DOX) catalyzing the primary oxygenation of fatty acids to oxylipins were recently found in plants. Here, the biological roles of the pepper α-DOX (Ca-DOX) gene, which is strongly induced during non-host pathogen infection in chili pepper, were examined. Virus-induced gene silencing demonstrated t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0575-3
更新日期:2017-03-01 00:00:00
abstract::Soybean is an important crop not only for human consumption but also for its addition of nitrogen to the soil during crop rotation. China has the largest collection of cultivated soybeans (Glycine max) and wild soybeans (Glycine soja) all over the world. The platform of soybean core, mini core and integrated applied c...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-013-0076-6
更新日期:2013-09-01 00:00:00