Abstract:
:The soluble acyl-ACP:sn-glycerol-3-phosphate acyltransferase from chloroplasts of chilling-sensitive and -resistant plants differ in their fatty acid selectivity. Enzymes from resistant plants discriminate against non-fluid palmitic acid and select oleic acid whereas the acyltransferase from sensitive plants accepts both fatty acids. To use this difference for improving plant chilling resistance by biotechnology the gene for an oleate-selective enzyme is required. Therefore, the oleate-selective enzyme from pea seedlings was purified to apparent homogeneity. Tryptic peptides of internal origin were sequenced. Polyclonal antibodies raised in rabbits were used for an immunological screening of a pea leaf cDNA expression library in lambda gt11. A positive clone of 1800 bp was selected showing an open reading frame which codes for 457 amino acids. The deduced amino acid sequence coincides perfectly with the tryptic sequences. A tentative assignment of the processing site was made which divides the preprotein into a mature protein of 41 kDa in accordance with experimental findings and a transit peptide of 88 amino acids. At present the comparison between a selective (pea) and an unselective (squash) acyltransferase sequence does not provide a clue for recognizing the structural differences resulting in different selectivities.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Weber S,Wolter FP,Buck F,Frentzen M,Heinz Edoi
10.1007/BF00037145subject
Has Abstractpub_date
1991-11-01 00:00:00pages
1067-76issue
5eissn
0167-4412issn
1573-5028journal_volume
17pub_type
杂志文章abstract::Plastidic acetyl-CoA carboxylase (ACCase) regulates the rate of fatty acid synthesis. This enzyme is composed of biotin carboxyl carrier protein (BCCP), biotin carboxylase (BC), and carboxyltransferase (CT), which consists of α and β subunits. Among these components, CTβ is encoded by the plastidic genome. In Arabidop...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0075-7
更新日期:2013-07-01 00:00:00
abstract::We have sequenced a plastid gene cluster from the unicellular red alga Cyanidium caldarium which is located downstream from the psbA gene and contains, in the following order, genes for a beta-allophycocyanin-like protein (apcB'), a putative 9.5 kDa allophycocyanin linker protein (apcL9.5) and a putative 29 kDa phycoc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014494
更新日期:1992-10-01 00:00:00
abstract::The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019103
更新日期:1996-11-01 00:00:00
abstract::Leaves develop as flat lateral organs from the indeterminate shoot apical meristem. The establishment of polarity along three-dimensional axes, proximal-distal, medial-lateral, and adaxial-abaxial axes, is crucial for the growth of normal leaves. The mutations of ASYMMETRIC LEAVES1 (AS1) and AS2 of Arabidopsis thalian...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9929-7
更新日期:2012-08-01 00:00:00
abstract:KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00926-7
更新日期:2020-01-01 00:00:00
abstract::Left- and right-border repeats, which surround the T-region, contain two conserved regions separated by 5 bp that are not conserved. At the onset of T-DNA processing virD-encoded proteins introduce a nick in the largest of these conserved regions (12 bp) at a specific position in the bottom strand between a guanine an...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027312
更新日期:1989-11-01 00:00:00
abstract::Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine. In plants GS is an octameric enzyme and is located either in the cytoplasm (GS1) or in the chloroplast (GS2). Two distinct classes of GS1 genes with unique 3'-untranslated region (3'UTR) have been identified ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006099512706
更新日期:1998-06-01 00:00:00
abstract:KEY MESSAGE:The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, howeve...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00848-4
更新日期:2019-05-01 00:00:00
abstract:KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0516-1
更新日期:2016-10-01 00:00:00
abstract::A clone encoding a proline-rich protein (ZmPRP) has been obtained from maize root by differential screening of a maturing elongation root cDNA library. The amino acid sequence deduced from the full-length cDNA contains a putative signal peptide and a highly repetitive sequence containing the PEPK motif, indicating tha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006129703262
更新日期:1999-03-01 00:00:00
abstract::Anaerobiosis rapidly induces alcohol dehydrogenase (ADH), an enzyme of the fermentation pathway, in different parts of rice seedlings. After initiation of anaerobiosis, the activity of the enzyme increases linearly for 3 days or more. The ADH activity is anaerobically inducible even in mature rice leaves in contrast t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027335
更新日期:1989-07-01 00:00:00
abstract::The gene coding for a new class of proteins rich in glycine and proline (GPRP) was cloned in Arabidopsis thaliana. In the protein sequence, five amino acids - glycine, proline, alanine, tyrosine and histidine - account for 79.4% of the total composition. The protein has two different glycine-rich domains interrupted b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00049336
更新日期:1996-02-01 00:00:00
abstract:KEY MESSAGE:Overexpression of BoMYB29 gene up-regulates the aliphatic glucosinolate pathway in Brassica oleracea plants increasing the production of the anti-cancer metabolite glucoraphanin, and the toxic and pungent sinigrin. Isothiocyanates, the bio-active hydrolysis products of glucosinolates, naturally produced by ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00890-2
更新日期:2019-09-01 00:00:00
abstract::The synthesis of both strands of CaMV-DNA has been studied in vitro using viral replication complexes obtained by hypotonic extraction of infected plant organelles. Hybridization of the DNA synthesized in vitro to single stranded CaMV DNA probes cloned in bacteriophage M 13 confirmed that the 35 S RNA served as a temp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017779
更新日期:1984-09-01 00:00:00
abstract::The High Mobility Group (HMG) proteins of vertebrate animals have been the subject of intensive study because of evidence that they may be structural proteins of transcriptionally active chromatin. Organisms other than vertebrate animals have chromatin proteins which meet the operational criteria of salt extractabilit...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015875
更新日期:1987-09-01 00:00:00
abstract::A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015653
更新日期:1990-01-01 00:00:00
abstract::Plants produce ethylene in response to many biotic and abiotic stresses. In response to ozone the foliage of potato plants sequentially expressed two ACC synthase genes (ST-ACS4, ST-ACS5). The same expression pattern of the two genes also occurred in response to Cu2+ and infection with Alternaria solani. ST-ACS5 expre...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005857717196
更新日期:1997-12-01 00:00:00
abstract::In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-loc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005736722794
更新日期:1997-04-01 00:00:00
abstract::Gene expression for nuclear- and mitochondrial-encoded subunits of respiratory chain components was measured in developing soybean cotyledons and compared to the abundance of the relevant proteins. Overall respiratory gene expression peaked at day 16, close to the peak in cytochrome chain and TCA cycle activities from...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1022502501373
更新日期:2003-03-01 00:00:00
abstract::We have previously shown that the mitochondrial gene atpA, encoding the alpha subunit of F1 ATP synthase, is associated with DNA rearrangements and nuclear-specific transcript patterns in the male-sterile cytoplasm of Ogura radish. Here we present a detailed characterization of this gene from both the normal (fertile)...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016123
更新日期:1990-11-01 00:00:00
abstract::The soybean leghemoglobin lba gene promoter sequence was determined and aligned with the promoter sequence of the soybean lbc3 gene from the same gene family. Five highly conserved regions were found. There are two large conserved regions, one of which overlaps the basic promoter while the other defines a minimal enha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027380
更新日期:1993-08-01 00:00:00
abstract::We have identified two single-copy genes from the model legume. Medicago truncatula (MtENOD16 and 20) whose expression can be correlated with early stages of root nodulation and whose predicted coding sequences are partially homologous to both pea/vetch ENOD5 and soybean N315/ENOD55. Database searching and sequence al...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005916821224
更新日期:1998-03-01 00:00:00
abstract::Plants are capable to de novo synthesize the essential amino acids leucine, isoleucine and valine. Studies in recent years, however, also revealed that plants have the potential to degrade leucine or may be all of the branched-chain amino acids. One of the enzymes participating in both biosynthesis and degradation is ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-7533-1
更新日期:2005-01-01 00:00:00
abstract::RbcL and rbcS mRNA levels and plastid transcription activity are low in the basal meristematic region of barley primary leaves and increase coordinately during leaf cell development with a similar time course in dark-grown or illuminated seedlings. The capacity of light to cause cab mRNA accumulation shows a similar d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037063
更新日期:1991-10-01 00:00:00
abstract::The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-013-0038-z
更新日期:2013-08-01 00:00:00
abstract::The auxin-binding protein isolated from maize coleoptiles and characterized in detail describes an auxin recognition protein at the outer surface of the plasmalemma which mediates the auxin effect on cell elongation in maize coleoptiles. Its homologue in tobacco mesophyll protoplasts mediates the auxin effect on secre...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00019401
更新日期:1990-06-01 00:00:00
abstract:KEY MESSAGE:Leveraging the heightened levels of polymorphism in NB-ARC-related protein encoding genes in higher plants, a bioinformatic pipeline was created to identify regions in this gene family from sequenced plant genomes that exhibit fragment length or single nucleotide differences in different accessions of the s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0774-1
更新日期:2018-10-01 00:00:00
abstract::A vector has been designed that contains a truncated CaMV (cauliflower mosaic virus) 35S promoter fused to a receptor gene encoding beta-glucuronidase (GUS), placed adjacent to the left border sequence of an Agrobacterium vector. In potato plants transformed with this vector, different patterns of transcription were d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020557
更新日期:1991-02-01 00:00:00
abstract::Cistus creticus subsp. creticus is a plant of intrinsic scientific interest due to the distinctive pharmaceutical properties of its resin. Labdane-type diterpenes, the main constituents of the resin, exhibit considerable antibacterial and cytotoxic activities. In this study chemical analysis of isolated trichomes from...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9399-0
更新日期:2008-12-01 00:00:00
abstract::Selective gene expression in different populations of cells of the root apex of transgenic tobacco could be evidenced by means of GUS constructs with deletions of the rolB promoter and fusions with the CaMV 35S minimal promoter. Five regulatory regions have been broadly identified in the rolB 5' non-coding region. The...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029606
更新日期:1994-07-01 00:00:00