A transcript encoding translation initiation factor eIF-5A is stored in unfertilized egg cells of maize.

abstract：:We isolated a rice cDNA encoding nucleoside diphosphate kinase (NDK, EC 2.7.4.6). The deduced amino acid sequence of the rice NDK shows highest homology to spinach NDK-I. The rice NDK gene exhibits a strong codon bias (73.8% GC) in the third position of the codon. DNA blot analysis indicated that at least single NDK g...

journal_title：Plant molecular biology

authors： Yano A,Shimazaki T,Kato A,Umeda M,Uchimiya H

更新日期：1993-12-01 00:00:00

abstract：:The 22 kDa auxin-binding proteins in higher plants have received considerable attention as candidates for an auxin receptor. A cDNA clone Ca-ERabp1 of hot pepper (Capsicum annum) was isolated using the oligonucleotides as PCR primers. The cDNA codes for a polypeptide related to the major 22 kDa auxin-binding protein f...

journal_title：Plant molecular biology

authors： Choi SY

更新日期：1996-12-01 00:00:00

abstract：:We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...

journal_title：Plant molecular biology

authors： Wu H,Echt CS,Popp MP,Davis JM

更新日期：1997-04-01 00:00:00

abstract：:Full-length cDNAs encoding the alpha- and beta-subunits and a truncated mutant subunit of the Chlorella sorokiniana NADP-GDH isozymes were constructed and expressed in Escherichia coli cells. The kinetic and thermal stability properties of the resultant homohexamers were examined. The electrophoretic mobility of the r...

journal_title：Plant molecular biology

authors： Lawit SJ,Miller PW,Dunn WI,Mirabile JS,Schmidt RR

更新日期：2003-06-01 00:00:00

abstract：:A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...

journal_title：Plant molecular biology

authors： Leisy DJ,Hnilo J,Zhao Y,Okita TW

更新日期：1990-01-01 00:00:00

abstract：:The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number...

journal_title：Plant molecular biology

authors： Müller BS,Sakamoto T,de Menezes IP,Prado GS,Martins WS,Brondani C,de Barros EG,Vianello RP

更新日期：2014-11-01 00:00:00

abstract：:Complementary DNA clones encoding acidic and basic isoforms of the class III chitinase were isolated from Nicotiana tabacum. The clones share ca. 65% identity, are equally homologous to the class III chitinases from cucumber and Arabidopsis, and are members of small gene families in tobacco. An acidic class III chitin...

journal_title：Plant molecular biology

authors： Lawton K,Ward E,Payne G,Moyer M,Ryals J

更新日期：1992-08-01 00:00:00

abstract：:Opium poppy latex contains a group of laticifer-specific, low-molecular-weight polypeptides called major latex proteins (MLPs). Here we describe a new member of the MLP gene family (gMLP 22) which shares 79.6% nucleotide and 84.6% amino acid sequence identity with previously isolated clones. DNA gel blot analysis indi...

journal_title：Plant molecular biology

authors： Nessler CL,Burnett RJ

更新日期：1992-11-01 00:00:00

abstract：:We report the isolation of the second member, kin2, of a family of two cold-inducible genes of Arabidopsis thaliana. The proteins corresponding to the two genes have similarities to the small antifreeze proteins from Winter flounder. Kin1 and kin2 are organized in a close tandem array in the genome of A. thaliana. Bot...

journal_title：Plant molecular biology

authors： Kurkela S,Borg-Franck M

更新日期：1992-07-01 00:00:00

abstract：KEY MESSAGE:KEG is involved in mediating the proteasome-dependent degradation of FDH, a stress-responsive enzyme. The UPS may function to suppress FDH mediated stress responses under favorable growth conditions. Formate dehydrogenase (FDH) has been studied in bacteria and yeasts for the purpose of industrial applicatio...

journal_title：Plant molecular biology

authors： McNeilly D,Schofield A,Stone SL

更新日期：2018-02-01 00:00:00

abstract：:The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized protein that specificall...

journal_title：Plant molecular biology

authors： Liu C,Mao B,Ou S,Wang W,Liu L,Wu Y,Chu C,Wang X

更新日期：2014-01-01 00:00:00

abstract：:The shoot apical meristem of higher plants is characterized by a conserved pattern of cell division, the functional significance of which is unclear. Although a causal role for cell division frequency and orientation in morphogenesis has been suggested, supporting data are limited. An alternative interpretation laying...

journal_title：Plant molecular biology

authors： Fleming AJ

更新日期：2006-04-01 00:00:00

abstract：:We have constructed a chimaeric gene consisting of the promoter of the soybean heat shock (hs) gene Gmhsp17, 6-L, the coding region of a hygromycin phosphotransferase (hpt) gene, and the termination sequence of the nopaline synthase (nos) gene. This gene fusion was introduced into tobacco by Agrobacterium-mediated gen...

journal_title：Plant molecular biology

authors： Severin K,Schöffl F

更新日期：1990-12-01 00:00:00

abstract：:The interaction between a protein and DNA is involved in almost all cellular functions, and is vitally important in cellular processes. Two complementary approaches are used to detect the interactions between a transcription factor (TF) and DNA, i.e. the TF-centered or protein-DNA approach, and the gene-centered or DN...

journal_title：Plant molecular biology

authors： Ji X,Wang L,Nie X,He L,Zang D,Liu Y,Zhang B,Wang Y

更新日期：2014-11-01 00:00:00

abstract：:The identification of some nodule-specific host proteins (nodulins) from common bean (Phaseolus vulgaris L.), a tropical ureide-transporting legume, is described. Particularly, the existence and developmental expression of several abundant nodule-specific transcripts of P. vulgaris are shown, including leghemoglobin, ...

journal_title：Plant molecular biology

authors： Campos F,Padilla J,Vázquez M,Ortega JL,Enríquez C,Sánchez F

更新日期：1987-11-01 00:00:00

abstract：:The large number of mitogen-activated protein (MAP) kinase genes identified to date in plants suggests that their encoded proteins have a wide array of functions in development and physiological responses, as has been indicated by studies on the factors which lead to the activation of these kinases. Signalling pathway...

journal_title：Plant molecular biology

authors： Voronin V,Touraev A,Kieft H,van Lammeren AA,Heberle-Bors E,Wilson C

更新日期：2001-04-01 00:00:00

abstract：:A genomic library was prepared from Populus kitakamiensis and screened with the cDNA for an anionic peroxidase from P. kitakamiensis. One genomic clone was isolated that contained two tandemly oriented genes for anionic peroxidases, prxA3a and prxA4a. Both genes consisted of four exons and three introns; the introns h...

journal_title：Plant molecular biology

authors： Osakabe K,Koyama H,Kawai S,Katayama Y,Morohoshi N

更新日期：1995-07-01 00:00:00

abstract：:Gibberellins (GAs) are important plant growth regulators, regulating many plant developmental processes, including seed germination, root and stem elongation, rosette expansion, floral induction and anther development. The diverse effects of GAs on plant development make it critical to maintain an appropriate endogeno...

journal_title：Plant molecular biology

authors： Zhang Y,Schwarz S,Saedler H,Huijser P

更新日期：2007-02-01 00:00:00

abstract：:An 8 bp sequence repeated 6 times is present to the right of the mannopine type pRi8196 T-DNA right-border sequence. Experiments were designed to test whether these repeats have a role in T-DNA transfer. Several constructs in which different lengths of pRi8196 right-border region were linked to the cucumopine synthesi...

journal_title：Plant molecular biology

authors： Hansen G,Tempé J,Brevet J

更新日期：1992-10-01 00:00:00

abstract：:Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...

journal_title：Plant molecular biology

authors： Burrell MM,Temple S,Ooms G

更新日期：1986-07-01 00:00:00

abstract：:We have isolated and analysed a 2 kb region of the mitochondrial genome of Arabidopsis thaliana (Columbia) showing a high level of nucleotide identity with the mitochondrial (mt) rps14 small-subunit ribosomal protein gene from Oenothera berteriana and Vicia faba, as well as with an open reading frame (ORF) located ups...

journal_title：Plant molecular biology

authors： Aubert D,Bisanz-Seyer C,Herzog M

更新日期：1992-12-01 00:00:00

abstract：:A highly purified RNA polymerase preparation from pea chloroplasts has been shown to specifically transcribe the 16S rRNA gene in vitro using the recombinant pCB2-8 DNA as a template. The RNA polymerase has been found to show maximum activity and specificity with pea supercoiled rDNA as a template. At low concentratio...

journal_title：Plant molecular biology

authors： Sun E,Shapiro DR,Wu BW,Tewari KK

更新日期：1986-11-01 00:00:00

abstract：:The synthesis, modification, and breakdown of carbohydrates is one of the most fundamentally important reactions in nature. The structural and functional diversity of glycosides is mirrored by a vast array of enzymes involved in their synthesis (glycosyltransferases), modification (carbohydrate esterases) and breakdow...

journal_title：Plant molecular biology

authors： Henrissat B,Coutinho PM,Davies GJ

更新日期：2001-09-01 00:00:00

abstract：KEY MESSAGE:Two OsDGAT1 genes showed the ability to restore TAG and LB synthesis in yeast H1246. Alterations in the N-terminal region of OsDGAT1-1 gene revealed its regulatory role in gene function. Accumulation of triacylglycerol (TAG) or oil in vegetative tissues has emerged as a promising approach to meet the global...

journal_title：Plant molecular biology

authors： Bhunia RK,Sinha K,Chawla K,Randhawa V,Sharma TR

更新日期：2020-10-21 00:00:00

abstract：:Repetitive DNA sequences were detected directly on somatic metaphase chromosome spreads from soybean root tips using fluorescent in situ hybridization. Methods to spread the forty small metaphase chromosomes substantially free of cellular material were developed using protoplasts. The specific DNA probe was a 1.05 kb ...

journal_title：Plant molecular biology

authors： Griffor MC,Vodkin LO,Singh RJ,Hymowitz T

更新日期：1991-07-01 00:00:00

abstract：:We report the isolation and characterisation of a wound-induced cDNA designated AoPR1 from a suspension of mesophyll cells that had been mechanically isolated from cladodes of light-grown Asparagus officinalis seedlings by grinding in a mortar and pestle. The transcript abundance is up-regulated following cell separat...

journal_title：Plant molecular biology

authors： Warner SA,Scott R,Draper J

更新日期：1992-07-01 00:00:00

abstract：:The regulation of genes in response to wounding is mediated in part by the octadecanoids 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA) and its methyl ester methyl jasmonate (MeJA). We identified, by differential display, an Arabidopsis gene (OPR3) induced after wounding. OPR3 is homologous to members of the flav...

journal_title：Plant molecular biology

authors： Costa CL,Arruda P,Benedetti CE

更新日期：2000-09-01 00:00:00

abstract：:In previous work, a 33 kDa cysteine proteinase was found in callus initiated from maize (Zea mays L.) resistant to fall armyworm feeding. A callus cDNA library from the maize inbred Mp708 was screened with oligonucleotides derived from the N-terminal amino acid sequence of the 33 kDa proteinase and several cDNA clones...

journal_title：Plant molecular biology

authors： Pechan T,Jiang B,Steckler D,Ye L,Lin L,Luthe DS,Williams WP

更新日期：1999-05-01 00:00:00

abstract：:Pathogens induce the expression of many genes encoding plant transcription factors, though specific knowledge of the biological function of individual transcription factors remains scarce. NAC transcription factors are encoded in plants by a gene family with proposed functions in both abiotic and biotic stress adaptat...

journal_title：Plant molecular biology

authors： Jensen MK,Rung JH,Gregersen PL,Gjetting T,Fuglsang AT,Hansen M,Joehnk N,Lyngkjaer MF,Collinge DB

更新日期：2007-09-01 00:00:00

abstract：:The red algal chloroplast genome encodes an essential prokaryotic cell division gene, ftsH, which has never been found in the mitochondrial genome of any organism. To compare the conserved prokaryote-derived mechanism for mitochondrial division with that of chloroplasts, we cloned chloroplast- and nuclear-encoded ftsH...

journal_title：Plant molecular biology

authors： Itoh R,Takano H,Ohta N,Miyagishima S,Kuroiwa H,Kuroiwa T

更新日期：1999-10-01 00:00:00