An alternative approach for gene transfer in trees using wild-type Agrobacterium strains.


:Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on walnut, gall formation was weak. On poplar and wild cherry, tumors induced by nopaline strains developed spontaneously shoots that had a normal phenotype and did not carry oncogenic T-DNA. From these observations, we have established a co-inoculation method to transform plants, using poplar as an experimental model. The method is based on inoculation of stem internodes with an Agrobacterium suspension containing both an oncogenic strain that induces shoot differentiation and a disarmed strain that provides the suitable genes in a binary vector. We used the vector pBI121 carrying neo (kanamycin resistance) and uidA (beta-glucuronidase) genes to facilitate early selection and screening. Poplar plants derived from kanamycin-resistant shoots that did not carry oncogenic T-DNA, were shown to contain and to express neo and uidA genes. These results suggest that wild-type Agrobacterium strains that induce shoot formation directly from tumors can be used as a general tool for gene transfer, avoiding difficult regeneration procedures.


Plant Mol Biol


Plant molecular biology


Brasileiro AC,Leplé JC,Muzzin J,Ounnoughi D,Michel MF,Jouanin L




Has Abstract


1991-09-01 00:00:00












  • Chloroplastic isoforms of DnaJ and GrpE in pea.

    abstract::The folding and assembly of proteins in cells often requires the assistance of molecular chaperones such as the Hsp70 and Hsp60 heat shock proteins. Hsp70 chaperones cooperate with DnaJ and GrpE homologues to ensure a productive folding cycle. In this study we describe the gene for the first chloroplast localized DnaJ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Schlicher T,Soll J

    更新日期:1997-01-01 00:00:00

  • The small auxin-up RNA OsSAUR45 affects auxin synthesis and transport in rice.

    abstract:KEY MESSAGE:This research is the first to demonstrate that OsSAUR45 is involved in plant growth though affecting auxin synthesis and transport by repressing OsYUCCA and OsPIN gene expression in rice. Small auxin-up RNAs (SAURs) comprise a large multigene family and are rapidly activated as part of the primary auxin res...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Xu YX,Xiao MZ,Liu Y,Fu JL,He Y,Jiang DA

    更新日期:2017-05-01 00:00:00

  • A genetic genomics-expression approach reveals components of the molecular mechanisms beyond the cell wall that underlie peach fruit woolliness due to cold storage.

    abstract::Peach fruits subjected to prolonged cold storage (CS) to delay decay and over-ripening often develop a form of chilling injury (CI) called mealiness/woolliness (WLT), a flesh textural disorder characterized by lack of juiciness. Transcript profiles were analyzed after different lengths of CS and subsequent shelf life ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Pons C,Martí C,Forment J,Crisosto CH,Dandekar AM,Granell A

    更新日期:2016-11-01 00:00:00

  • Organization of the major latex protein gene family in opium poppy.

    abstract::Opium poppy latex contains a group of laticifer-specific, low-molecular-weight polypeptides called major latex proteins (MLPs). Here we describe a new member of the MLP gene family (gMLP 22) which shares 79.6% nucleotide and 84.6% amino acid sequence identity with previously isolated clones. DNA gel blot analysis indi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Nessler CL,Burnett RJ

    更新日期:1992-11-01 00:00:00

  • Hygromycin B-induced cell death is partly mediated by reactive oxygen species in rice (Oryza sativa L.).

    abstract::The aminoglycoside antibiotic hygromycin B (Hyg) inhibits prokaryotic, chloroplast and mitochondrial protein synthesis. Because of the toxic effect of Hyg on plant cells, the HPT gene, encoding hygromycin phosphotransferase, has become one of the most widely used selectable markers in plant transformation. Yet the mec...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Oung HM,Lin KC,Wu TM,Chandrika NN,Hong CY

    更新日期:2015-12-01 00:00:00

  • Suppression of phenylalanine ammonia lyase expression in sugar beet by the fungal pathogen Cercospora beticola is mediated at the core promoter of the gene.

    abstract::The suppression of plant defence reactions plays a crucial role in causing plant diseases. In this report, we show that inducible plant defences are repressed during the development of Cercospora leaf spot disease. In the early phase of infection of sugar beet (Beta vulgaris L.) leaves with the phytopathogenic fungus ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Schmidt K,Heberle B,Kurrasch J,Nehls R,Stahl DJ

    更新日期:2004-08-01 00:00:00

  • Unexpectedly higher expression levels of a chimeric 2S albumin seed protein transgene from a tandem array construct.

    abstract::In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expressi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Conceição Ada S,Van Vliet A,Krebbers E

    更新日期:1994-11-01 00:00:00

  • Microsequencing and cDNA cloning of the Calvin cycle/OPPP enzyme ribose-5-phosphate isomerase (EC from spinach chloroplasts.

    abstract::Ribose-5-phosphate isomerase (RPI) catalyses the interconversion of ribose-5-phosphate and ribulose-5-phosphate in the reductive and oxidative pentose phosphate pathways in plants. RPI from spinach chloroplasts was purified and microsequenced. Via PCR with degenerate primers designed against microsequenced peptides, a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Martin W,Henze K,Kellerman J,Flechner A,Schnarrenberger C

    更新日期:1996-02-01 00:00:00

  • Differential ethylene-inducible expression of cellulase in pepper plants.

    abstract::Ethylene promotes the abscission of leaves and the ripening of fruits in pepper plants, and in both events an increase in cellulase activity is observed. However, two enzyme isoforms (pI 7.2 and 8.5, respectively) are differentially involved in the two physiological phenomena. The pI 8.5 form has been purified from ri...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Ferrarese L,Trainotti L,Moretto P,Polverino de Laureto P,Rascio N,Casadoro G

    更新日期:1995-11-01 00:00:00

  • A survey of transcripts expressed specifically in root nodules of broadbean (Vicia faba L.).

    abstract::More than 600 potentially nodule-specific clones have been detected by differential hybridization of a broadbean cDNA library constructed from root nodule poly(A)+ RNA. These isolated cDNAs belong to at least 28 different clone groups containing cross-hybridizing sequences. The number of clones within a clone group va...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Perlick AM,Pühler A

    更新日期:1993-09-01 00:00:00

  • Structure and expression of a cDNA encoding a histone H2A from Euglena gracilis.

    abstract::Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a pol...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Saint-Guily A,Schantz ML,Schantz R

    更新日期:1994-03-01 00:00:00

  • Characterization of radish mitochondrial atpA: influence of nuclear background on transcription of atpA-associated sequences and relationship with male sterility.

    abstract::We have previously shown that the mitochondrial gene atpA, encoding the alpha subunit of F1 ATP synthase, is associated with DNA rearrangements and nuclear-specific transcript patterns in the male-sterile cytoplasm of Ogura radish. Here we present a detailed characterization of this gene from both the normal (fertile)...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Makaroff CA,Apel IJ,Palmer JD

    更新日期:1990-11-01 00:00:00

  • Degradation of the stress-responsive enzyme formate dehydrogenase by the RING-type E3 ligase Keep on Going and the ubiquitin 26S proteasome system.

    abstract:KEY MESSAGE:KEG is involved in mediating the proteasome-dependent degradation of FDH, a stress-responsive enzyme. The UPS may function to suppress FDH mediated stress responses under favorable growth conditions. Formate dehydrogenase (FDH) has been studied in bacteria and yeasts for the purpose of industrial applicatio...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: McNeilly D,Schofield A,Stone SL

    更新日期:2018-02-01 00:00:00

  • Purification, immunological characterization and cDNA cloning of a 47 kDa glycoprotein secreted by carrot suspension cells.

    abstract::A 47 kDa glycoprotein, termed EP4, was purified from carrot cell suspension culture medium. An antiserum raised against EP4 also recognized a protein of 45 kDa that was ionically bound to the cell wall. EP4 was detected in culture media from both embryogenic and non-embryogenic cell lines and was found to be secreted ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: van Engelen FA,de Jong AJ,Meijer EA,Kuil CW,Meyboom JK,Dirkse WG,Booij H,Hartog MV,Vandekerckhove J,de Vries SC

    更新日期:1995-03-01 00:00:00

  • Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression.

    abstract::We describe a method for identifying signal transducing proteins from other organisms by their ability to turn on a signalling pathway when they are expressed at high level in yeast. The method was tested on a cDNA library from Arabidopsis thaliana, which was screened for clones that can activate glucose repression in...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Thelander M,Fredriksson D,Schouten J,Hoge JH,Ronne H

    更新日期:2002-05-01 00:00:00

  • DORNRÖSCHEN-LIKE expression marks Arabidopsis floral organ founder cells and precedes auxin response maxima.

    abstract::Live imaging during floral development revealed that expression of the DORNRÖSCHEN-LIKE (DRNL) gene encoding an AP2-like transcription factor, marks all organ founder cells. Transcription precedes the perception of auxin response maxima as measured by the DR5 reporter and is unaffected in early organogenesis, by mutat...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Chandler JW,Jacobs B,Cole M,Comelli P,Werr W

    更新日期:2011-05-01 00:00:00

  • Non-photosynthetic 'malic enzyme' from maize: a constituvely expressed enzyme that responds to plant defence inducers.

    abstract::The characterization of a non-photosynthetic isoform of NADP-malic enzyme (NADP-ME) from maize roots, which represents nearly 7% of the total soluble protein of this tissue, was performed. The molecular properties of the purified protein, as well as the kinetic parameters determined, indicate that the NADP-ME isoform ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Maurino VG,Saigo M,Andreo CS,Drincovich MF

    更新日期:2001-03-01 00:00:00

  • Expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid.

    abstract::When subjected to salt stress or drought, some vascular plants such as barley respond with an increased accumulation of the osmoprotectant glycine betaine (betaine), being the last step of betaine synthesis catalyzed by betaine aldehyde dehydrogenase (BADH). We report here cloning and characterization of BADH cDNA fro...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Ishitani M,Nakamura T,Han SY,Takabe T

    更新日期:1995-01-01 00:00:00

  • Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogen

    abstract::A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Bak S,Kahn RA,Nielsen HL,Moller BL,Halkier BA

    更新日期:1998-02-01 00:00:00

  • Identification and characterization of AtCASP, a plant transmembrane Golgi matrix protein.

    abstract::Golgins are a family of coiled-coil proteins that are associated with the Golgi apparatus. They are necessary for tethering events in membrane fusion and may act as structural support for Golgi cisternae. Here we report on the identification of an Arabidopsis golgin which is a homologue of CASP, a known transmembrane ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Renna L,Hanton SL,Stefano G,Bortolotti L,Misra V,Brandizzi F

    更新日期:2005-05-01 00:00:00

  • Stress responses in alfalfa (Medicago sativa L.) 11. Molecular cloning and expression of alfalfa isoflavone reductase, a key enzyme of isoflavonoid phytoalexin biosynthesis.

    abstract::The major phytoalexin in alfalfa is the isoflavonoid (-)-medicarpin (or 6aR, 11aR)-medicarpin. Isoflavone reductase (IFR), the penultimate enzyme in medicarpin biosynthesis, is responsible for introducing one of two chiral centers in (-)-medicarpin. We have isolated a 1.18 kb alfalfa cDNA (pIFRalf1) which, when expres...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Paiva NL,Edwards R,Sun YJ,Hrazdina G,Dixon RA

    更新日期:1991-10-01 00:00:00

  • Transgenic Arabidopsis expressing osmolyte glycine betaine synthesizing enzymes from halophilic methanogen promote tolerance to drought and salt stress.

    abstract::Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Lai SJ,Lai MC,Lee RJ,Chen YH,Yen HE

    更新日期:2014-07-01 00:00:00

  • Isolation of a cDNA encoding an Arabidopsis galactokinase by functional expression in yeast.

    abstract::A cDNA clone encoding Arabidopsis thaliana galactokinase was fortuitously isolated during the course of a screen for plant homologues of a Saccharomyces cerevisiae peroxisome assembly gene, PAS9. Clones were sought which restored the ability of pas9 cells to grow on oleate as a sole carbon source, as oleate metabolism...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Kaplan CP,Tugal HB,Baker A

    更新日期:1997-06-01 00:00:00

  • Early genomic responses to salicylic acid in Arabidopsis.

    abstract::Salicylic acid (SA) is a stress-induced hormone involved in the activation of defense genes. Here we analyzed the early genetic responses to SA of wild type and npr1-1 mutant Arabidopsis seedlings, using Complete Arabidopsis Transcriptome MicroArray (CATMAv2) chip. We identified 217 genes rapidly induced by SA (early ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Blanco F,Salinas P,Cecchini NM,Jordana X,Van Hummelen P,Alvarez ME,Holuigue L

    更新日期:2009-05-01 00:00:00

  • Isolation and characterization of P-type H(+)-ATPase genes from potato.

    abstract::H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tiss...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Harms K,Wöhner RV,Schulz B,Frommer WB

    更新日期:1994-11-01 00:00:00

  • PCR-generated cDNA library of transition-stage maize embryos: cloning and expression of calmodulin genes during early embryogenesis.

    abstract::One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 x 10(5) recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Breton C,Chaboud A,Matthys-Rochon E,Bates EE,Cock JM,Fromm H,Dumas C

    更新日期:1995-01-01 00:00:00

  • Molecular characterization of His-Asp phosphorelay signaling factors in maize leaves: implications of the signal divergence by cytokinin-inducible response regulators in the cytosol and the nuclei.

    abstract::Genes for histidyl-aspartyl (His-Asp) phosphorelay components (His-containing phosphotransfer proteins, HP, and response regulators, RR) were isolated from Zea mays L. to characterize their function in cytokinin signaling. Six type-A RRs (ZmRR1, ZmRR2, ZmRR4-ZmRR7), 3 type-B RRs (ZmRR8-ZmRR10), and 3 HPs (ZmHP1-ZmHP3)...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Asakura Y,Hagino T,Ohta Y,Aoki K,Yonekura-Sakakibara K,Deji A,Yamaya T,Sugiyama T,Sakakibara H

    更新日期:2003-05-01 00:00:00

  • Hormone interactions during vascular development.

    abstract::Vascular tissue in plants is unique due to its diverse and dynamic cellular patterns. Signals controlling vascular development have only recently started to emerge through biochemical, genetic, and genomic approaches in several organisms, such as Arabidopsis, Populus, and Zinnia. These signals include hormones (auxin,...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Dettmer J,Elo A,Helariutta Y

    更新日期:2009-03-01 00:00:00

  • Two ftsH-family genes encoded in the nuclear and chloroplast genomes of the primitive red alga Cyanidioschyzon merolae.

    abstract::The red algal chloroplast genome encodes an essential prokaryotic cell division gene, ftsH, which has never been found in the mitochondrial genome of any organism. To compare the conserved prokaryote-derived mechanism for mitochondrial division with that of chloroplasts, we cloned chloroplast- and nuclear-encoded ftsH...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Itoh R,Takano H,Ohta N,Miyagishima S,Kuroiwa H,Kuroiwa T

    更新日期:1999-10-01 00:00:00

  • Structure and expression of a gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (rbcL) in the colourless euglenoid flagellate Astasia longa.

    abstract::A gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) was identified on a circular 73 kb DNA from the colourless euglenoid flagellate Astasia longa. The rbcL gene of Astasia extends over 3968 bp. It is a split gene interrupted by seven introns as compared to nine intervening sequences in...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Siemeister G,Hachtel W

    更新日期:1990-05-01 00:00:00