Abstract：
:Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on walnut, gall formation was weak. On poplar and wild cherry, tumors induced by nopaline strains developed spontaneously shoots that had a normal phenotype and did not carry oncogenic T-DNA. From these observations, we have established a co-inoculation method to transform plants, using poplar as an experimental model. The method is based on inoculation of stem internodes with an Agrobacterium suspension containing both an oncogenic strain that induces shoot differentiation and a disarmed strain that provides the suitable genes in a binary vector. We used the vector pBI121 carrying neo (kanamycin resistance) and uidA (beta-glucuronidase) genes to facilitate early selection and screening. Poplar plants derived from kanamycin-resistant shoots that did not carry oncogenic T-DNA, were shown to contain and to express neo and uidA genes. These results suggest that wild-type Agrobacterium strains that induce shoot formation directly from tumors can be used as a general tool for gene transfer, avoiding difficult regeneration procedures.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Brasileiro AC,Leplé JC,Muzzin J,Ounnoughi D,Michel MF,Jouanin Ldoi
10.1007/BF00040638subject
Has Abstractpub_date
1991-09-01 00:00:00pages
441-52issue
3eissn
0167-4412issn
1573-5028journal_volume
17pub_type
杂志文章abstract：:Peach fruits subjected to prolonged cold storage (CS) to delay decay and over-ripening often develop a form of chilling injury (CI) called mealiness/woolliness (WLT), a flesh textural disorder characterized by lack of juiciness. Transcript profiles were analyzed after different lengths of CS and subsequent shelf life ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0526-z
更新日期：2016-11-01 00:00:00
abstract：:As a first step toward elucidating the in vivo function of plant bZIP proteins and their related G-box cis elements, we have introduced a dominant negative inhibitor of G-box-dependent transcriptional activation into tobacco plants by transforming them with a truncated EmBP-1 gene (deltaEmBP) containing the DNA bindin...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006081009173
更新日期：1998-11-01 00:00:00
abstract：:The 22 kDa auxin-binding proteins in higher plants have received considerable attention as candidates for an auxin receptor. A cDNA clone Ca-ERabp1 of hot pepper (Capsicum annum) was isolated using the oligonucleotides as PCR primers. The cDNA codes for a polypeptide related to the major 22 kDa auxin-binding protein f...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020496
更新日期：1996-12-01 00:00:00
abstract：:Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0460-0
更新日期：2016-06-01 00:00:00
abstract：:We have cloned and sequenced a wound-inducible cDNA clone designated WIP1 (for wound-induced protein) from maize coleoptiles. It was isolated by differential screening of a cDNA library prepared from excised maize coleoptile segments. The deduced amino acid sequence predicts a secretory, cysteine-rich protein of 102 r...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027365
更新日期：1993-08-01 00:00:00
abstract：:Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9428-z
更新日期：2009-02-01 00:00:00
abstract：:A genomic library was prepared from Populus kitakamiensis and screened with the cDNA for an anionic peroxidase from P. kitakamiensis. One genomic clone was isolated that contained two tandemly oriented genes for anionic peroxidases, prxA3a and prxA4a. Both genes consisted of four exons and three introns; the introns h...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021193
更新日期：1995-07-01 00:00:00
abstract：:A method is described for the high frequency transformation of carrot proembryogenic suspension culture cells by a non-oncogenic Ti-plasmid vector (pGV3850::1103) which carried a chimaeric kanamycin resistance gene (nos-NPT-II). Plants were regenerated efficiently from transformed material by somatic embryogenesis in ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015034
更新日期：1987-05-01 00:00:00
abstract：:Repetitive DNA sequences were detected directly on somatic metaphase chromosome spreads from soybean root tips using fluorescent in situ hybridization. Methods to spread the forty small metaphase chromosomes substantially free of cellular material were developed using protoplasts. The specific DNA probe was a 1.05 kb ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00036810
更新日期：1991-07-01 00:00:00
abstract：:A seedling lethal mutant of Nicotiana plumbaginifolia (sdl-1) was isolated by transposon tagging using a maize Dissociation (Ds) element. The insertion mutation was produced by direct co-transformation of protoplasts with two plasmids: one containing Ds and a second with an Ac transposase gene. sdl-1 seedlings exhibit...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1019851913083
更新日期：2002-10-01 00:00:00
abstract：:Free Air [CO(2)] Enrichment (FACE) allows for plant growth under fully open-air conditions of elevated [CO(2)] at concentrations expected to be reached by mid-century. We used Arabidopsis thaliana ecotypes Col-0, Cvi-0, and WS to analyze changes in gene expression and metabolite profiles of plants grown in "SoyFACE" (...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9041-y
更新日期：2006-11-01 00:00:00
abstract：:Considerable progress in tomato molecular biology has been made over the past five years. At least 19 different mRNAs which increase in amount during tomato fruit ripening have been cloned and genes for enzymes involved in cell wall degradation (polygalacturonase and pectinesterase) and ethylene synthesis (ACC synthas...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/BF00015607
更新日期：1992-05-01 00:00:00
abstract：:We investigated the spatial and temporal expression patterns of two rice calcium-dependent protein kinases (CDPKs), OsCDPK2 and OSCDPK11, using isoform-specific antisera. Bands of the expected molecular sizes for OsCDPK2 (59 kDa) and OsCDPK11 (61 kDa) were detected on western blots. OsCDPK2 and OsCDPK11 mRNA and prote...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006316422400
更新日期：1999-12-01 00:00:00
abstract：KEY MESSAGE:OsHIRP1 is an E3 ligase that acts as a positive regulator in the plant response to heat stress, thus providing important information relating to adaptation and regulation under heat stress in plant. Extreme temperature adversely affects plant growth, development, and productivity. Here, we report the molecu...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00835-9
更新日期：2019-04-01 00:00:00
abstract：:The barley cysteine proteinase B (EPB) is the main protease responsible for the degradation of endosperm storage proteins providing nitrogenous nutrients to support the growth of young seedlings. The expression of this enzyme is induced in the germinating seeds by the phytohormone, gibberellin, and suppressed by anoth...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021787
更新日期：1996-05-01 00:00:00
abstract：:A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and -II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 4...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020464
更新日期：1995-12-01 00:00:00
abstract：:Miniature inverted-repeat transposable elements (MITEs) are short, non autonomous DNA elements that are widespread and abundant in plant genomes. The high sequence and size conservation observed in many MITE families suggest that they have spread recently throughout their respective host genomes. Here we present a mai...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9511-0
更新日期：2009-09-01 00:00:00
abstract：:The shoot apical meristem of higher plants is characterized by a conserved pattern of cell division, the functional significance of which is unclear. Although a causal role for cell division frequency and orientation in morphogenesis has been suggested, supporting data are limited. An alternative interpretation laying...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-005-7703-9
更新日期：2006-04-01 00:00:00
abstract：:We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027351
更新日期：1992-05-01 00:00:00
abstract：:Genes for histidyl-aspartyl (His-Asp) phosphorelay components (His-containing phosphotransfer proteins, HP, and response regulators, RR) were isolated from Zea mays L. to characterize their function in cytokinin signaling. Six type-A RRs (ZmRR1, ZmRR2, ZmRR4-ZmRR7), 3 type-B RRs (ZmRR8-ZmRR10), and 3 HPs (ZmHP1-ZmHP3)...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1023971315108
更新日期：2003-05-01 00:00:00
abstract：:The expression of chloroplast genes during cotton embryogenesis was followed by measuring hybridization of cloned chloroplast genomic fragments to total RNA immobilzed on membrane filters. RNA was extracted from the cotyledons of cotton embryos at different stages of development ranging from very small embryos (5 mg) ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040137
更新日期：1986-03-01 00:00:00
abstract：:The suppression of plant defence reactions plays a crucial role in causing plant diseases. In this report, we show that inducible plant defences are repressed during the development of Cercospora leaf spot disease. In the early phase of infection of sugar beet (Beta vulgaris L.) leaves with the phytopathogenic fungus ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-2141-7
更新日期：2004-08-01 00:00:00
abstract：:The molecular mechanism of signal transduction pathways which mediate the action of phytohormones are poorly understood. Recently, we and others have shown that the as -1 type cis-acting elements can respond to auxin and salicylic acid, two well-characterized signaling molecules in plants. In the present work, we have...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019093
更新日期：1996-11-01 00:00:00
abstract：:A cDNA with sequence similarity to isocitrate lyase (ICL) genes was isolated from the unicellular eukaryotic green alga Chlamydomonas reinhardtii as a light-induced mRNA in the carotenoid biosynthetic mutant strain FN68. The 416 amino acid open reading frame shows significant sequence similarity to isocitrate lyases o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005728411921
更新日期：1997-02-01 00:00:00
abstract：:When dark-grown aurea mutant tomato seedlings which lack more than 95% of the phytochrome present in isogenic wild-type seedlings are kept in white or blue light, four nuclear-encoded transcripts coding for plastidic proteins (the light-harvesting chlorophyll a/b-binding protein of photosystem I and II [cab-PSII], pla...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020560
更新日期：1991-02-01 00:00:00
abstract：:A leaf cDNA library from hybrid aspen, Populus tremula x tremuloides, was constructed. From this two different cDNA clones, denoted CA1a and CA1b, encoding a chloroplastic carbonic anhydrase (CA) were isolated and DNA sequenced. Analysis of the deduced amino acid sequences showed that the isolated CAs belong to the be...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005849202731
更新日期：1997-07-01 00:00:00
abstract：:Plastids possess a bacteria-like sec apparatus that is involved in protein import into the thylakoid lumen. We have analyzed one of the genes essential for this process, secY. A secY gene from the unicellular red alga Cyanidium caldarium was found to be transcriptionally active, demonstrating for the first time that s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020209
更新日期：1996-11-01 00:00:00
abstract：:In this report we characterized the Arabidopsis ABI1 gene orthologue and Brassica napus gene paralogues encoding protein phosphatase 2C (PP2C, group A), which is known to be a negative regulator of the ABA signaling pathway. Six homologous B. napus sequences were identified and characterized as putative PP2C group A m...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-015-0334-x
更新日期：2015-07-01 00:00:00
abstract：KEY MESSAGE:This review contains functional roles of MYB transcription factors in the transcriptional regulation of anthocyanin biosynthesis in horticultural plants. This review describes potential uses of MYB TFs as tools for metabolic engineering for anthocyanin production. Anthocyanins (ranging from red to blue) are...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-018-0771-4
更新日期：2018-09-01 00:00:00
abstract：:In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0244-3
更新日期：2014-11-01 00:00:00
