Transformation of carrot tissues derived from proembryogenic suspension cells: A useful model system for gene expression studies in plants.

abstract：:The phytohormone abscisic acid is probably present in all higher plants. This hormone is necessary for regulation of several events during seed development and for the response to environmental stresses such as desiccation, salt and cold. An important part of the physiological response to abscisic acid is achieved thr...

journal_title：Plant molecular biology

authors： Busk PK,Pagès M

更新日期：1998-06-01 00:00:00

abstract：KEY MESSAGE:To explore the function of Dof transcription factors during kernel development in maize, we first identified Dof genes in the maize genome. We found that ZmDof3 was exclusively expressed in the endosperm of maize kernel and had the features of a Dof transcription factor. Suppression of ZmDof3 resulted in a ...

journal_title：Plant molecular biology

authors： Qi X,Li S,Zhu Y,Zhao Q,Zhu D,Yu J

更新日期：2017-01-01 00:00:00

abstract：:The immunoscreening method was used to isolate cDNAs of 1323 bp (ClOCT1) and 1433 bp (ClOCT2) encoding two ornithine carbamoyltransferases (OCT, EC 2.1.3.3) from the cDNA expression library of Canavalia lineata leaves constructed in a lambdaZAP Express vector. ClOCT1 and ClOCT2 encode 359 and 369 amino acids, respecti...

journal_title：Plant molecular biology

authors： Lee Y,Choi YA,Hwang ID,Kim SG,Kwon YM

更新日期：2001-08-01 00:00:00

abstract：:The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...

journal_title：Plant molecular biology

authors： Bhunia RK,Chakraborty A,Kaur R,Gayatri T,Bhattacharyya J,Basu A,Maiti MK,Sen SK

更新日期：2014-11-01 00:00:00

abstract：:Sequences encoding the immunoglobulin heavy-chain variable (VH) domains were engineered in a new general purpose vector to transform plants via Agrobacterium. The expression of an isolated VH domain (IVD) after introduction into the plant genome has been monitored by northern, western and immunohistochemical analysis....

journal_title：Plant molecular biology

authors： Benvenuto E,Ordàs RJ,Tavazza R,Ancora G,Biocca S,Cattaneo A,Galeffi P

更新日期：1991-10-01 00:00:00

abstract：:Catalase 2 (CAT2) plays an important role in the detoxification of hydrogen peroxide released either during photorespiration or as a consequence of biotic and abiotic stress as well as in the initiation of senescence. To date, our understanding of the regulation of CAT2 gene expression is rather poor. Chromatin immuno...

journal_title：Plant molecular biology

authors： Laxa M

更新日期：2017-01-01 00:00:00

abstract：:Knowledge of the genes in gymnosperms encoding the apoproteins of the plant photoreceptor phytochrome is currently scanty as for gymnosperm nuclear protein coding sequences in general. Here we report two complete cDNA-derived sequences which code for two different types of gymnosperm phytochrome. One sequence stems fr...

journal_title：Plant molecular biology

authors： Clapham DH,Kolukisaoglu HU,Larsson CT,Qamaruddin M,Ekberg I,Wiegmann-Eirund C,Schneider-Poetsch HA,von Arnold S

更新日期：1999-07-01 00:00:00

abstract：:Identification of downstream target genes of stress-relating transcription factors (TFs) is desirable in understanding cellular responses to various environmental stimuli. However, this has long been a difficult work for both experimental and computational practices. In this research, we presented a novel computationa...

journal_title：Plant molecular biology

authors： Wang S,Yang S,Yin Y,Guo X,Wang S,Hao D

更新日期：2009-01-01 00:00:00

abstract：:Suppressive subtractive hybridisation was applied to the analysis of late stage arbuscular mycorrhizal development in pea. 96 cDNA clones were amplified and 81, which carried fragments more than 200 nt in size, were sequence analysed. Among 67 unique fragments, 10 showed no homology and 10 were similar to sequences wi...

journal_title：Plant molecular biology

authors： Grunwald U,Nyamsuren O,Tamasloukht M,Lapopin L,Becker A,Mann P,Gianinazzi-Pearson V,Krajinski F,Franken P

更新日期：2004-07-01 00:00:00

abstract：:Ethylene promotes the abscission of leaves and the ripening of fruits in pepper plants, and in both events an increase in cellulase activity is observed. However, two enzyme isoforms (pI 7.2 and 8.5, respectively) are differentially involved in the two physiological phenomena. The pI 8.5 form has been purified from ri...

journal_title：Plant molecular biology

authors： Ferrarese L,Trainotti L,Moretto P,Polverino de Laureto P,Rascio N,Casadoro G

更新日期：1995-11-01 00:00:00

abstract：:Using a 2,4-dichlorophenoxyacetic acid (2,4-D)-dependent tobacco cell-suspension culture we analyzed early hormone-induced molecular events preceding cell division. By differential screening of a cDNA library to mRNAs derived from hormone-starved cells treated with 2,4-D for 4 h, seven non-cross hybridizing cDNA clone...

journal_title：Plant molecular biology

authors： van der Zaal EJ,Memelink J,Mennes AM,Quint A,Libbenga KR

更新日期：1987-03-01 00:00:00

abstract：:We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...

journal_title：Plant molecular biology

authors： Edwards KJ,Thompson H,Edwards D,de Saizieu A,Sparks C,Thompson JA,Greenland AJ,Eyers M,Schuch W

更新日期：1992-05-01 00:00:00

abstract：:Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...

journal_title：Plant molecular biology

authors： Jackson MT

更新日期：1997-09-01 00:00:00

abstract：KEY MESSAGE:Overexpression of BoMYB29 gene up-regulates the aliphatic glucosinolate pathway in Brassica oleracea plants increasing the production of the anti-cancer metabolite glucoraphanin, and the toxic and pungent sinigrin. Isothiocyanates, the bio-active hydrolysis products of glucosinolates, naturally produced by ...

journal_title：Plant molecular biology

authors： Zuluaga DL,Graham NS,Klinder A,van Ommen Kloeke AEE,Marcotrigiano AR,Wagstaff C,Verkerk R,Sonnante G,Aarts MGM

更新日期：2019-09-01 00:00:00

abstract：:Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultiva...

journal_title：Plant molecular biology

authors： Wenck AR,Quinn M,Whetten RW,Pullman G,Sederoff R

更新日期：1999-02-01 00:00:00

abstract：:Eukaryotes express several cytoplasmic HSP70 genes, and their encoded proteins participate in diverse cellular processes. Three cDNAs encoding highly expressed cytoplasmic HSP70 homologues from Pisum sativum were cloned and characterized. They were designated PsHSP71.2, PsHSC71.0, and PsHSP70b. These HSP70 genes have ...

journal_title：Plant molecular biology

authors： DeRocher A,Vierling E

更新日期：1995-02-01 00:00:00

abstract：:14-3-3 proteins form a family of highly conserved proteins with central roles in many eukaryotic signalling networks. In plants, they bind to and activate the plasma membrane H+-ATPase, creating a binding site for the phytotoxin fusicoccin. Barley 14-3-3 transcripts accumulate in the epidermis upon inoculation with th...

journal_title：Plant molecular biology

authors： Finni C,Andersen CH,Borch J,Gjetting S,Christensen AB,de Boer AH,Thordal-Christensen H,Collinge DB

更新日期：2002-05-01 00:00:00

abstract：:The Arabidopsis genome contains 10 genes belonging to the HD-Zip II family including ATHB2 and HAT2. Previous work has shown that ATHB2 is rapidly and strongly induced by light quality changes that provoke the shade avoidance response whereas HAT2 expression responds to auxin. Here, we present a genome-wide analysis o...

journal_title：Plant molecular biology

authors： Ciarbelli AR,Ciolfi A,Salvucci S,Ruzza V,Possenti M,Carabelli M,Fruscalzo A,Sessa G,Morelli G,Ruberti I

更新日期：2008-11-01 00:00:00

abstract：:Plants that utilize the highly efficient C4 photosynthetic pathway possess two types of specialized leaf cells, the mesophyll and bundle sheath. In mature leaves of amaranth, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (Rubisco) is localized specifically to the chloroplasts of bundle sheath cells,...

journal_title：Plant molecular biology

authors： Boinski JJ,Wang JL,Xu P,Hotchkiss T,Berry JO

更新日期：1993-06-01 00:00:00

abstract：:The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...

journal_title：Plant molecular biology

authors： Huss B,Tinland B,Paulus F,Walter B,Otten L

更新日期：1990-02-01 00:00:00

abstract：:Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a m...

journal_title：Plant molecular biology

authors： Irish EE,McMurray D

更新日期：2006-03-01 00:00:00

abstract：:Complex signal transduction pathways underlie the myriad plant responses to attack by pathogens. Ca(2+) is a universal second messenger in eukaryotes that modulates various signal transduction pathways through stimulus-specific changes in its intracellular concentration. Ca(2+)-binding proteins such as calmodulin (CaM...

journal_title：Plant molecular biology

authors： Chiasson D,Ekengren SK,Martin GB,Dobney SL,Snedden WA

更新日期：2005-08-01 00:00:00

abstract：KEY MESSAGE:In response to an ultrasound pulse, several hundred DEGs, including in response to stress, were up- or down-regulated in in vitro potato plantlets. Despite this abiotic stress, plantlets survived. Ultrasound (US) can influence plant growth and development. To better understand the genetic mechanism underlyi...

journal_title：Plant molecular biology

authors： Dobránszki J,Hidvégi N,Gulyás A,Teixeira da Silva JA

更新日期：2019-07-01 00:00:00

abstract：:Tight control of cell/tissue identity is essential for a correct and functional organ patterning, an important component of overall fruit development and eventual maturation and ripening. Despite many investigations regarding the molecular determinants of cell identity in fruits of different species, a useful model ab...

journal_title：Plant molecular biology

authors： Botton A,Rasori A,Ziliotto F,Moing A,Maucourt M,Bernillon S,Deborde C,Petterle A,Varotto S,Bonghi C

更新日期：2016-05-01 00:00:00

abstract：:A homologue of the multidrug resistance (MDR) gene was obtained while screening a potato stolon tip cDNA expression library with 35S-labeled calmodulin. The mammalian MDR gene codes for a membrane-bound P-glycoprotein (170-180 kDa) which imparts multidrug resistance to cancerous cells. The potato cDNA (PMDR1) codes fo...

journal_title：Plant molecular biology

authors： Wang W,Takezawa D,Poovaiah BW

更新日期：1996-06-01 00:00:00

abstract：:cDNA clones for all enzymes of the prechorismate pathway of higher plants have previously been cloned, with the exception of the second enzyme of the pathway, 3-dehydroquinate synthase. Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-d...

journal_title：Plant molecular biology

authors： Bischoff M,Rösler J,Raesecke HR,Görlach J,Amrhein N,Schmid J

更新日期：1996-04-01 00:00:00

abstract：:A drought- and abscisic acid (ABA)-inducible gene, His1, was isolated from Lycopersicon pennellii, a drought-resistant relative of cultivated tomato, and the gene structure was defined experimentally. The nucleotide sequence of His1 predicts a protein of 202 amino acid residues, with a significant sequence homology to...

journal_title：Plant molecular biology

authors： Wei T,O'Connell MA

更新日期：1996-01-01 00:00:00

abstract：KEY MESSAGE:Hybrid abnormalities, severe growth abortion and grass-clump dwarfism, were found in the tetraploid wheat/Aegilops umbellulata hybrids, and the gene expression changes were conserved in the hybrids with those in other wheat synthetic hexaploids. Aegilops umbellulata Zhuk., a diploid goatgrass species with a...

journal_title：Plant molecular biology

authors： Okada M,Yoshida K,Takumi S

更新日期：2017-12-01 00:00:00

abstract：:The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the ch...

journal_title：Plant molecular biology

authors： Wu H,Liu XQ

更新日期：1997-05-01 00:00:00

abstract：:It has been demonstrated that insertion of foreign DNA into mammalian genome can profoundly alter the patterns of DNA methylation and transcription of the host genome. Introgression of alien DNA into plant genomes through sexual crossing and genetic engineering are commonly used in breeding, but it is not known if pla...

journal_title：Plant molecular biology

authors： Liu Z,Wang Y,Shen Y,Guo W,Hao S,Liu B

更新日期：2004-03-01 00:00:00