The complex structures of arabinogalactan-proteins and the journey towards understanding function.

abstract：:The synthesis of both strands of CaMV-DNA has been studied in vitro using viral replication complexes obtained by hypotonic extraction of infected plant organelles. Hybridization of the DNA synthesized in vitro to single stranded CaMV DNA probes cloned in bacteriophage M 13 confirmed that the 35 S RNA served as a temp...

journal_title：Plant molecular biology

authors： Pfeiffer P,Laquel P,Hohn T

更新日期：1984-09-01 00:00:00

abstract：:A tapetum-specific gene, RTS, has been isolated by differential screening of a cDNA library from rice panicles. RTS is a unique gene in the rice genome. RNA blot analysis and in situ hybridization indicates that this gene is predominantly expressed in the anther's tapetum during meiosis and disappears before anthesis....

journal_title：Plant molecular biology

authors： Luo H,Lee JY,Hu Q,Nelson-Vasilchik K,Eitas TK,Lickwar C,Kausch AP,Chandlee JM,Hodges TK

更新日期：2006-10-01 00:00:00

abstract：:A drought- and abscisic acid (ABA)-inducible gene, His1, was isolated from Lycopersicon pennellii, a drought-resistant relative of cultivated tomato, and the gene structure was defined experimentally. The nucleotide sequence of His1 predicts a protein of 202 amino acid residues, with a significant sequence homology to...

journal_title：Plant molecular biology

authors： Wei T,O'Connell MA

更新日期：1996-01-01 00:00:00

abstract：:T-DNA tagging is a high throughput strategy for identifying and cloning functional genes in plants. In this study, we screened 4416 lab-created T(1) rice T-DNA tagged lines and identified a mutant, designated dh1 (degenerated hull1), with phenotype of degenerated hull and naked pistils and stamens. Approximately 60% f...

journal_title：Plant molecular biology

authors： Li A,Zhang Y,Wu X,Tang W,Wu R,Dai Z,Liu G,Zhang H,Wu C,Chen G,Pan X

更新日期：2008-03-01 00:00:00

abstract：:Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases ot...

journal_title：Plant molecular biology

authors： Trapy G,Favre A,Esnault R

更新日期：1981-03-01 00:00:00

abstract：:Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA...

journal_title：Plant molecular biology

authors： Wang H,Guo Y,Lv F,Zhu H,Wu S,Jiang Y,Li F,Zhou B,Guo W,Zhang T

更新日期：2010-03-01 00:00:00

abstract：:Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...

journal_title：Plant molecular biology

authors： Jackson MT

更新日期：1997-09-01 00:00:00

abstract：:Alpha-dioxygenases (α-DOX) catalyzing the primary oxygenation of fatty acids to oxylipins were recently found in plants. Here, the biological roles of the pepper α-DOX (Ca-DOX) gene, which is strongly induced during non-host pathogen infection in chili pepper, were examined. Virus-induced gene silencing demonstrated t...

journal_title：Plant molecular biology

authors： Hong CE,Ha YI,Choi H,Moon JY,Lee J,Shin AY,Park CJ,Yoon GM,Kwon SY,Jo IH,Park JM

更新日期：2017-03-01 00:00:00

abstract：KEY MESSAGE:In this manuscript, we disclosed the influence of light on the accumulation of storage reserves in B. napus embryos.1.Light induced the gene expression in the developing embryos of B. napus.2.Light promoted the starch synthesis in chloroplasts of B. napus embryos.3.Light enhanced the metabolic activity of s...

journal_title：Plant molecular biology

authors： Tan H,Qi X,Li Y,Wang X,Zhou J,Liu X,Shi X,Ye W,Xiang X

更新日期：2020-07-01 00:00:00

abstract：:To test the influence of a Nicotiana tomentosiformis repetitive sequence (R8.3) on transgene expression in N. sylvestris and in N. sylvestris-N. tomentosiformis hybrids, the R8.3 sequence was placed upstream of a nopaline synthase promoter (NOSpro)-NPTII reporter gene in a T-DNA construct. A number of transgenic N. sy...

journal_title：Plant molecular biology

authors： Kunz C,Narangajavana J,Jakowitsch J,Park YD,Delon TR,Kovarik A,Koukalová B,van der Winden J,Moscone E,Aufsatz W,Mette MF,Matzke M,Matzke AJ

更新日期：2003-05-01 00:00:00

abstract：:The single-copy gene encoding the Arabidopsis HMG-I/Y protein was isolated and characterised. The gene encodes a protein of 204 amino acid residues and contains a single intron of 73 bp. Primer extension analysis indicates that transcription starts 115 bp upstream of the translation start and the leader sequence conta...

journal_title：Plant molecular biology

authors： Gupta R,Webster CI,Gray JC

更新日期：1998-04-01 00:00:00

abstract：:Persian walnut (Juglans regia L.) is an economically important tree for its nut crop and timber. To gain insight into the structure and evolution of the walnut genome, we constructed two bacterial artificial chromosome (BAC) libraries, containing a total of 129,024 clones, from in vitro-grown shoots of J. regia cv. Ch...

journal_title：Plant molecular biology

authors： Wu J,Gu YQ,Hu Y,You FM,Dandekar AM,Leslie CA,Aradhya M,Dvorak J,Luo MC

更新日期：2012-01-01 00:00:00

abstract：:Left- and right-border repeats, which surround the T-region, contain two conserved regions separated by 5 bp that are not conserved. At the onset of T-DNA processing virD-encoded proteins introduce a nick in the largest of these conserved regions (12 bp) at a specific position in the bottom strand between a guanine an...

journal_title：Plant molecular biology

authors： van Haaren MJ,Sedee NJ,de Boer HA,Schilperoort RA,Hooykaas PJ

更新日期：1989-11-01 00:00:00

abstract：:The transcript level and in turn protein density of the K(+)-uptake channel ZMK1 in maize (Zea mays) coleoptiles is controlled by the phytohormone auxin. ZMK1 is involved in auxin-regulated coleoptile elongation as well as gravi- and phototropism. To provide unequivocal evidence for the role of ZMK1 in these elementar...

journal_title：Plant molecular biology

authors： Philippar K,Büchsenschütz K,Edwards D,Löffler J,Lüthen H,Kranz E,Edwards KJ,Hedrich R

更新日期：2006-07-01 00:00:00

abstract：:Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...

journal_title：Plant molecular biology

authors： Esfandiari E,Jin Z,Abdeen A,Griffiths JS,Western TL,Haughn GW

更新日期：2013-01-01 00:00:00

abstract：:We studied the early events of de novo formation of adventitious shoot meristems in stem segments of Brassica oleracea. A regeneration system was used that is efficient, rapid, highly responsive to cytokinins and does not involve callus formation, thus allowing studies on a direct developmental switch of cells in the ...

journal_title：Plant molecular biology

authors： Teo WL,Kumar P,Goh CJ,Swarup S

更新日期：2001-07-01 00:00:00

abstract：:Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but...

journal_title：Plant molecular biology

authors： Sheen JY,Sayre RT,Bogorad L

更新日期：1987-05-01 00:00:00

abstract：:In this survey of 5074 plant genes for their AUG context sequences, purines are present at the -3 and +4 positions in about 80% of the sequences. Although this observation is similar to the vertebrate consensus sequence, the number of plant mRNAs with purines at the -3 position is lower and at the +4 position is highe...

journal_title：Plant molecular biology

authors： Joshi CP,Zhou H,Huang X,Chiang VL

更新日期：1997-12-01 00:00:00

abstract：:Plants perceiving drought activate multiple responses to improve survival, including large-scale alterations in gene expression. This article reports on the roles in the drought response of two Arabidopsis thaliana homeodomain-leucine zipper class I genes; ATHB7 and ATHB12, both strongly induced by water-deficit and a...

journal_title：Plant molecular biology

authors： Valdés AE,Overnäs E,Johansson H,Rada-Iglesias A,Engström P

更新日期：2012-11-01 00:00:00

abstract：:Plant cells respond to cold stress via a regulatory mechanism leading to enhanced cold acclimation accompanied by growth retardation. The C-repeat binding factor (CBF) signaling pathway is essential for cold response of flowering plants. Our previously study documented a novel CBF-like gene from the cold-tolerant Caps...

journal_title：Plant molecular biology

authors： Zhou M,Xu M,Wu L,Shen C,Ma H,Lin J

更新日期：2014-06-01 00:00:00

abstract：:The regulation of genes in response to wounding is mediated in part by the octadecanoids 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA) and its methyl ester methyl jasmonate (MeJA). We identified, by differential display, an Arabidopsis gene (OPR3) induced after wounding. OPR3 is homologous to members of the flav...

journal_title：Plant molecular biology

authors： Costa CL,Arruda P,Benedetti CE

更新日期：2000-09-01 00:00:00

abstract：:Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by...

journal_title：Plant molecular biology

authors： Ni Z,Hu Z,Jiang Q,Zhang H

更新日期：2013-05-01 00:00:00

abstract：:Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (T...

journal_title：Plant molecular biology

authors： Kim MJ,Ham BK,Kim HR,Lee IJ,Kim YJ,Ryu KH,Park YI,Paek KH

更新日期：2005-12-01 00:00:00

abstract：:During early pea fruit growth, the physiological roles of 4-chloroindole-3-acetic acid (4-Cl-IAA) and IAA, natural pea auxins, in regulating gibberellin (GA) 20-oxidase gene expression (PsGA20ox1) were tested with 4-position, ring-substituted auxins that have a range of biological activities (fruit growth). The effect...

journal_title：Plant molecular biology

authors： Ngo P,Ozga JA,Reinecke DM

更新日期：2002-07-01 00:00:00

abstract：:An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153. ...

journal_title：Plant molecular biology

authors： Riccardi G,De Rossi E,Della Valle G,Ciferri O

更新日期：1985-03-01 00:00:00

abstract：:The nuclear gene mutant of barley, vir-115, shows a developmentally induced loss of D1 synthesis that results in inactivation of Photosystem II. Translation in plastids isolated from 1 h illuminated vir-115 seedlings is similar to wild type. In wild-type barley, illumination of plants for 16 to 72 h results in increas...

journal_title：Plant molecular biology

authors： Kim J,Klein PG,Mullet JE

更新日期：1994-06-01 00:00:00

abstract：:The Arabidopsis genome contains 10 genes belonging to the HD-Zip II family including ATHB2 and HAT2. Previous work has shown that ATHB2 is rapidly and strongly induced by light quality changes that provoke the shade avoidance response whereas HAT2 expression responds to auxin. Here, we present a genome-wide analysis o...

journal_title：Plant molecular biology

authors： Ciarbelli AR,Ciolfi A,Salvucci S,Ruzza V,Possenti M,Carabelli M,Fruscalzo A,Sessa G,Morelli G,Ruberti I

更新日期：2008-11-01 00:00:00

abstract：:We have identified two single-copy genes from the model legume. Medicago truncatula (MtENOD16 and 20) whose expression can be correlated with early stages of root nodulation and whose predicted coding sequences are partially homologous to both pea/vetch ENOD5 and soybean N315/ENOD55. Database searching and sequence al...

journal_title：Plant molecular biology

authors： Greene EA,Erard M,Dedieu A,Barker DG

更新日期：1998-03-01 00:00:00

abstract：:Polyphenol oxidases (PPOs) catalyze the oxidation of ortho-diphenols to the corresponding quinones (EC 1.10.3.1). In plants PPOs appear in gene families, and the corresponding isoenzymes are located to the thylakoid lumen of chloroplasts. Although plant PPOs are often discussed with regard to their role in defense rea...

journal_title：Plant molecular biology

authors： Dirks-Hofmeister ME,Inlow JK,Moerschbacher BM

更新日期：2012-09-01 00:00:00

abstract：:Plant R2R3-MYB transcription factors are encoded by more than 100 copies of genes. In this study, we attempted to isolate some members of the R2R3-MYB superfamily involved in regulation of nitrogen fixation in legumes. A library of 300 recombinant plasmid clones containing the R2R3-MYB fragments of the superfamily was...

journal_title：Plant molecular biology

authors： Miyake K,Ito T,Senda M,Ishikawa R,Harada T,Niizeki M,Akada S

更新日期：2003-09-01 00:00:00