Identification of tomato Lhc promoter regions necessary for circadian expression.

abstract：:In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco th...

journal_title：Plant molecular biology

authors： Zhang H,Wu Q,Cao S,Zhao T,Chen L,Zhuang P,Zhou X,Gao Z

更新日期：2014-11-01 00:00:00

abstract：:A hybrid Cauliflower Mosaic Virus (CaMV) genome containing a selectable marker gene was constructed by replacing the gene VI coding region with the aminoglycoside (neomycin) phosphotransferase type II [APH(3')II] gene from Tn5. This modified viral genome was tested for its infectivity both in planta and in a protoplas...

journal_title：Plant molecular biology

authors： Paszkowski J,Pisan B,Shillito RD,Hohn T,Hohn B,Potrykus I

更新日期：1986-09-01 00:00:00

abstract：:Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on waln...

journal_title：Plant molecular biology

authors： Brasileiro AC,Leplé JC,Muzzin J,Ounnoughi D,Michel MF,Jouanin L

更新日期：1991-09-01 00:00:00

abstract：:Live imaging during floral development revealed that expression of the DORNRÖSCHEN-LIKE (DRNL) gene encoding an AP2-like transcription factor, marks all organ founder cells. Transcription precedes the perception of auxin response maxima as measured by the DR5 reporter and is unaffected in early organogenesis, by mutat...

journal_title：Plant molecular biology

authors： Chandler JW,Jacobs B,Cole M,Comelli P,Werr W

更新日期：2011-05-01 00:00:00

abstract：:Many studies in both animal and plant systems have shown that matrix attachment regions (MARs) can increase the expression of flanking transgenes. However, our previous studies revealed no effect of the chicken lysozyme MARs (chiMARs) on transgene expression in the first generation transgenic Arabidopsis thaliana plan...

journal_title：Plant molecular biology

authors： De Bolle MF,Butaye KM,Goderis IJ,Wouters PF,Jacobs A,Delauré SL,Depicker A,Cammue BP

更新日期：2007-03-01 00:00:00

abstract：:Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (...

journal_title：Plant molecular biology

authors： Diaz EC,Martin F,Tagu D

更新日期：1996-07-01 00:00:00

abstract：:A partial cDNA clone, Pis 63, corresponding to a mRNA highly expressed in Brassica napus pistils, was isolated by differential screening. PCR was used to complete the Pis 63 sequence (Pis 63-1) and to obtain the sequence of another related cDNA (Pis 63-2). Northern blot and in situ analyses demonstrated that these tra...

journal_title：Plant molecular biology

authors： Robert LS,Allard S,Gerster JL,Cass L,Simmonds J

更新日期：1994-11-01 00:00:00

abstract：:A cDNA library was made from poly(A)+ RNA isolated from developing Douglas fir (Pseudotsuga menziesii) embryo and megagametophytic tissue, and the cDNA clones were identified by immunoscreening with polyclonal antiserum against the crystalloid storage protein complex of Douglas fir. The nucleotide sequence of the long...

journal_title：Plant molecular biology

authors： Leal I,Misra S

更新日期：1993-02-01 00:00:00

abstract：:Plants that utilize the highly efficient C4 photosynthetic pathway possess two types of specialized leaf cells, the mesophyll and bundle sheath. In mature leaves of amaranth, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (Rubisco) is localized specifically to the chloroplasts of bundle sheath cells,...

journal_title：Plant molecular biology

authors： Boinski JJ,Wang JL,Xu P,Hotchkiss T,Berry JO

更新日期：1993-06-01 00:00:00

abstract：:Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could be detected only in Avicennia marina. Pinitol was the main osmoprotectant in the other three spec...

journal_title：Plant molecular biology

authors： Hibino T,Meng YL,Kawamitsu Y,Uehara N,Matsuda N,Tanaka Y,Ishikawa H,Baba S,Takabe T,Wada K,Ishii T,Takabe T

更新日期：2001-02-01 00:00:00

abstract：:Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases ot...

journal_title：Plant molecular biology

authors： Trapy G,Favre A,Esnault R

更新日期：1981-03-01 00:00:00

abstract：:In the wake of recent progress of high throughput transcriptome profiling technologies, extensive housekeeping gene mining has been conducted in humans. However, very few studies have been reported in maize (Zea mays L.), an important crop plant, and none were conducted on a genome -wide level. In this study, we surve...

journal_title：Plant molecular biology

authors： Lin F,Jiang L,Liu Y,Lv Y,Dai H,Zhao H

更新日期：2014-11-01 00:00:00

abstract：KEY MESSAGE:Psoralen synthase and angelicin synthase responsible for the formation of psoralen and angelicin in Peucedanum praeruptorum Dunn were identified and functionally characterized, respectively. Furanocoumarins were reported to possess several activities such as anticancer, anti-inflammatory and neuroprotective...

journal_title：Plant molecular biology

authors： Jian X,Zhao Y,Wang Z,Li S,Li L,Luo J,Kong L

更新日期：2020-10-01 00:00:00

abstract：KEY MESSAGE:OsGTγ-2, a trihelix transcription factor, is a positive regulator of rice responses to salt stress by regulating the expression of ion transporters. Salinity stress seriously restricts rice growth and yield. Trihelix transcription factors (GT factors) specifically bind to GT elements and play a diverse role...

journal_title：Plant molecular biology

authors： Liu X,Wu D,Shan T,Xu S,Qin R,Li H,Negm M,Wu D,Li J

更新日期：2020-07-01 00:00:00

abstract：:The molecular mechanism of signal transduction pathways which mediate the action of phytohormones are poorly understood. Recently, we and others have shown that the as -1 type cis-acting elements can respond to auxin and salicylic acid, two well-characterized signaling molecules in plants. In the present work, we have...

journal_title：Plant molecular biology

authors： Xiang C,Miao ZH,Lam E

更新日期：1996-11-01 00:00:00

abstract：:HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern ana...

journal_title：Plant molecular biology

authors： Agarwal M,Sahi C,Katiyar-Agarwal S,Agarwal S,Young T,Gallie DR,Sharma VM,Ganesan K,Grover A

更新日期：2003-03-01 00:00:00

abstract：:Full-length cDNAs encoding the alpha- and beta-subunits and a truncated mutant subunit of the Chlorella sorokiniana NADP-GDH isozymes were constructed and expressed in Escherichia coli cells. The kinetic and thermal stability properties of the resultant homohexamers were examined. The electrophoretic mobility of the r...

journal_title：Plant molecular biology

authors： Lawit SJ,Miller PW,Dunn WI,Mirabile JS,Schmidt RR

更新日期：2003-06-01 00:00:00

abstract：:The monoterpene indole alkaloids (MIAs) from Madagascar periwinkle (Catharanthus roseus) are secondary metabolites of high interest due to their therapeutical values. Secologanin, the monoterpenoid moiety incorporated into MIAs, is derived from the plastidial methyl-D: -erythritol 4-phosphate (MEP) pathway. Here, we h...

journal_title：Plant molecular biology

authors： Oudin A,Mahroug S,Courdavault V,Hervouet N,Zelwer C,Rodríguez-Concepción M,St-Pierre B,Burlat V

更新日期：2007-09-01 00:00:00

abstract：:Using a fruit-specific cDNA as a probe we isolated and sequenced the two corresponding homologous genes (Sn-1 and Sn-2) of the bell pepper (Capsicum annuum) genome. Both genes have a single intron and numerous unusual long inverted repeat sequences. The introns share 87% homology and Sn-2 contains one 450 bp additiona...

journal_title：Plant molecular biology

authors： Pozueta-Romero J,Klein M,Houlné G,Schantz ML,Meyer B,Schantz R

更新日期：1995-09-01 00:00:00

abstract：:Lectin receptor-like kinases (LecRLKs) are members of RLK family composed of lectin-like extracellular recognition domain, transmembrane domain and cytoplasmic kinase domain. LecRLKs are plasma membrane proteins believed to be involved in signal transduction. However, most of the members of the protein family even in ...

journal_title：Plant molecular biology

authors： Vaid N,Pandey P,Srivastava VK,Tuteja N

更新日期：2015-05-01 00:00:00

abstract：:The photosynthetic apparatus of plant chloroplasts contains two photosystems, termed Photosystem I (PSI) and Photosystem II (PSII). Both PSI and PSII contain several types of chlorophyll a/b-binding (CAB) polypeptides, at least some of which are structurally related. It has been previously shown that multiple genes en...

journal_title：Plant molecular biology

authors： Pichersky E,Hoffman NE,Malik VS,Bernatzky R,Tanksley SD,Szabo L,Cashmore AR

更新日期：1987-03-01 00:00:00

abstract：:The Arabidopsis ga1 mutant has very low levels of endogenous, active gibberellins and thus has an extreme dwarf phenotype; application of GA3 induces stem elongation and flower development. To test the hypothesis that GA action in this system involves changes in gene expression, we have cloned mRNAs whose abundance ch...

journal_title：Plant molecular biology

authors： Phillips AL,Huttly AK

更新日期：1994-02-01 00:00:00

abstract：:The nuclear gene mutant of barley, vir-115, shows a developmentally induced loss of D1 synthesis that results in inactivation of Photosystem II. Translation in plastids isolated from 1 h illuminated vir-115 seedlings is similar to wild type. In wild-type barley, illumination of plants for 16 to 72 h results in increas...

journal_title：Plant molecular biology

authors： Kim J,Klein PG,Mullet JE

更新日期：1994-06-01 00:00:00

abstract：KEY MESSAGE:TaPSK5 is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about t...

journal_title：Plant molecular biology

authors： Geng Y,Jian C,Xu W,Liu H,Hao C,Hou J,Liu H,Zhang X,Li T

更新日期：2020-12-01 00:00:00

abstract：:RNA editing of T-urf13, a gene specific to the mitochondria of cytoplasmic male-sterile, type-T (cms-T) maize, and an adjacent, cotranscribed gene orf221, have been studied by cDNA sequencing. No editing was detected in 22 cDNA clones. This is the only report of a polypeptide-encoding gene in higher-plant mitochondria...

journal_title：Plant molecular biology

authors： Ward GC,Levings CS 3rd

更新日期：1991-11-01 00:00:00

abstract：KEY MESSAGE:CYP71Z18 exhibited plastic substrate specificity to catalyze oxidation of multiple rice diterpenes and elevated chemical defense against the blast fungus in transgenic rice. Diversified plant specialized metabolism relies on corresponding biosynthetic enzymes with differential substrate specificity. CYP71Z1...

journal_title：Plant molecular biology

authors： Shen Q,Pu Q,Liang J,Mao H,Liu J,Wang Q

更新日期：2019-08-01 00:00:00

abstract：:We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...

journal_title：Plant molecular biology

authors： Edwards KJ,Thompson H,Edwards D,de Saizieu A,Sparks C,Thompson JA,Greenland AJ,Eyers M,Schuch W

更新日期：1992-05-01 00:00:00

abstract：:Starch-branching enzymes (SBE) alter starch structure by breaking an alpha-1,4 linkage and attaching the reducing end of the new chain to a glucan chain by an alpha 1,6 bond. In maize, three isoforms of SBE have been identified. In order to examine the function of the SBEI isoform, a reverse-genetics PCR-based screen ...

journal_title：Plant molecular biology

authors： Blauth SL,Kim KN,Klucinec J,Shannon JC,Thompson D,Guiltinan M

更新日期：2002-02-01 00:00:00

abstract：:Arabidopsis accessions are either winter-annuals, which require cold winter temperatures for spring flowering, or rapid-cycling summer annuals. Typically, winter annual accessions have functional FRIGIDA (FRI) and FRIGIDA-LIKE 1 (FRL1) proteins that promote high expression of FLOWERING LOCUS C (FLC), which prevents fl...

journal_title：Plant molecular biology

authors： Risk JM,Laurie RE,Macknight RC,Day CL

更新日期：2010-07-01 00:00:00

abstract：:Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...

journal_title：Plant molecular biology

authors： de Jager SM,Menges M,Bauer UM,Murra JA

更新日期：2001-11-01 00:00:00