Abstract:
:In bacterial and animal coenzyme A (CoA) biosynthesis, pantothenate kinase (PANK) activity is critical in regulating intracellular CoA levels. Less is known about the role of PANK in plants, although a single plant isozyme from Arabidopsis, AtPANK1, was previously cloned and analyzed in vitro. We report here the characterization of a second pantothenate kinase of Arabidopsis, AtPANK2, as well as characterization of the physiological roles of both plant enzymes. The activity of the second pantothenate kinase, AtPANK2, was confirmed by its ability to complement the temperature-sensitive mutation of the bacterial pantothenate kinase in E. coli strain ts9. Knock-out mutation of either AtPANK1 or AtPANK2 did not inhibit plant growth, whereas pank1-1/pank2-1 double knockout mutations were embryo lethal. The phenotypes of the mutant plants demonstrated that only one of the AtPANK enzymes is necessary and sufficient for producing adequate CoA levels, and that no other enzyme can compensate for the loss of both isoforms. Real-time PCR measurements of AtPANK1 and AtPANK2 transcripts indicated that both enzymes are expressed with similar patterns in all tissues examined, further suggesting that AtPANK1 and AtPANK2 have complementary roles. The two enzymes have homologous pantothenate kinase domains, but AtPANK2 also carries a large C-terminal protein domain. Sequence comparisons indicate that this type of "bifunctional" pantothenate kinase is conserved in other higher eukaryotes as well. Although the function of the C-terminal domain is not known, homology structure modeling suggests it contains a highly conserved cluster of charged residues that likely constitute a metal-binding site.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Tilton GB,Wedemeyer WJ,Browse J,Ohlrogge Jdoi
10.1007/s11103-006-0037-4subject
Has Abstractpub_date
2006-07-01 00:00:00pages
629-42issue
4-5eissn
0167-4412issn
1573-5028journal_volume
61pub_type
杂志文章abstract::Proline-rich protein (PRP), a cell wall protein of plant, has been studied in many plant species. Yet, none of the PRPs has been functionally elucidated. Here we report a novel flower-specific PRP designated OsPRP3 from rice. Expression analysis showed that the OsPRP3 transcript was mainly present in rice flower and a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9557-z
更新日期:2010-01-01 00:00:00
abstract::Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9866-x
更新日期:2012-02-01 00:00:00
abstract::The phytohormone abscisic acid is probably present in all higher plants. This hormone is necessary for regulation of several events during seed development and for the response to environmental stresses such as desiccation, salt and cold. An important part of the physiological response to abscisic acid is achieved thr...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006058700720
更新日期:1998-06-01 00:00:00
abstract:KEY MESSAGE:Overexpression of BoMYB29 gene up-regulates the aliphatic glucosinolate pathway in Brassica oleracea plants increasing the production of the anti-cancer metabolite glucoraphanin, and the toxic and pungent sinigrin. Isothiocyanates, the bio-active hydrolysis products of glucosinolates, naturally produced by ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00890-2
更新日期:2019-09-01 00:00:00
abstract::The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1026540524990
更新日期:2000-10-01 00:00:00
abstract::We have identified two single-copy genes from the model legume. Medicago truncatula (MtENOD16 and 20) whose expression can be correlated with early stages of root nodulation and whose predicted coding sequences are partially homologous to both pea/vetch ENOD5 and soybean N315/ENOD55. Database searching and sequence al...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005916821224
更新日期:1998-03-01 00:00:00
abstract::The cell cycle of eukaryotes is tightly regulated through the activity of cyclin-dependent kinases. The Arabidopsis thaliana CDKA;1 (CDC2aAt) gene is thought to encode such a protein kinase, since it is actively transcribed in proliferating tissues and can complement defects in the Schizosaccharomyces pombe cdc2 gene....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010665831955
更新日期:2001-05-01 00:00:00
abstract::The nucleotide sequence and the 5' flanking region of the rbcL gene coding for the large subunit of ribulose bisphosphate-1,5-carboxylase/oxygenase of Pylaiella littoralis, a brown alga, has been determined and the deduced amino-acid sequence has been compared to those of various photosynthetic and chemoautotrophic Eu...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00036916
更新日期:1990-08-01 00:00:00
abstract::Strigolactones have recently been identified as the long sought-after signal required to inhibit shoot branching (Gomez-Roldan et al. 2008; Umehara et al. 2008; reviewed in Dun et al. 2009). Here we briefly describe the evidence for strigolactone inhibition of shoot branching and, more extensively, the broader context...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-010-9599-2
更新日期:2010-05-01 00:00:00
abstract::More than 600 potentially nodule-specific clones have been detected by differential hybridization of a broadbean cDNA library constructed from root nodule poly(A)+ RNA. These isolated cDNAs belong to at least 28 different clone groups containing cross-hybridizing sequences. The number of clones within a clone group va...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028969
更新日期:1993-09-01 00:00:00
abstract::The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the ch...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005867215258
更新日期:1997-05-01 00:00:00
abstract::Plants are capable to de novo synthesize the essential amino acids leucine, isoleucine and valine. Studies in recent years, however, also revealed that plants have the potential to degrade leucine or may be all of the branched-chain amino acids. One of the enzymes participating in both biosynthesis and degradation is ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-7533-1
更新日期:2005-01-01 00:00:00
abstract::In a recent publication (Plant Molecular Biology 16: 547-565 (1991)), Showalter et al. described the isolation and initial characterization of fifteen extensin and extensin-like tomato cDNAs. These cDNAs were determined to fall into five distinct classes; class I and II clones encoded extensins, class III and V clones...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027342
更新日期:1992-05-01 00:00:00
abstract::Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine. In plants GS is an octameric enzyme and is located either in the cytoplasm (GS1) or in the chloroplast (GS2). Two distinct classes of GS1 genes with unique 3'-untranslated region (3'UTR) have been identified ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006099512706
更新日期:1998-06-01 00:00:00
abstract::Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00752901
更新日期:1986-07-01 00:00:00
abstract::We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040674
更新日期:1992-02-01 00:00:00
abstract::To understand the transcriptional responses to flooding stress in roots including hypocotyl of soybean seedlings, genome-wide changes in gene expression were analyzed using a soybean microarray chip containing 42,034 60-mer oligonucleotide probes. More than 6,000 of flooding-responsive genes in the roots including hyp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9799-4
更新日期:2011-09-01 00:00:00
abstract::The gene coding for a new class of proteins rich in glycine and proline (GPRP) was cloned in Arabidopsis thaliana. In the protein sequence, five amino acids - glycine, proline, alanine, tyrosine and histidine - account for 79.4% of the total composition. The protein has two different glycine-rich domains interrupted b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00049336
更新日期:1996-02-01 00:00:00
abstract::Plant cells respond to cold stress via a regulatory mechanism leading to enhanced cold acclimation accompanied by growth retardation. The C-repeat binding factor (CBF) signaling pathway is essential for cold response of flowering plants. Our previously study documented a novel CBF-like gene from the cold-tolerant Caps...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0181-1
更新日期:2014-06-01 00:00:00
abstract::In this survey of 5074 plant genes for their AUG context sequences, purines are present at the -3 and +4 positions in about 80% of the sequences. Although this observation is similar to the vertebrate consensus sequence, the number of plant mRNAs with purines at the -3 position is lower and at the +4 position is highe...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1005816823636
更新日期:1997-12-01 00:00:00
abstract::As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clone...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00024201
更新日期:1994-04-01 00:00:00
abstract::The barley BARE-1 is a transcribed, copia-like retroelement with well-conserved functional domains, an active promoter, and a copy number of at least 3 x 10(4). We examined its chromosomal localization by in situ hybridization. The long terminal repeat (LTR) probe displayed a uniform hybridization pattern over the who...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019563
更新日期:1996-03-01 00:00:00
abstract::To isolate genes which are expressed preferentially during embryogenesis, a Douglas-fir embryogenesis cDNA library was constructed and differentially screened with cDNA probes made with mRNA from developing and mature embryos, respectively. The cDNA clone PM 2.1 was isolated based on its abundance in developing seeds ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005839832096
更新日期:1997-05-01 00:00:00
abstract::The location on the wheat chloroplast DNA map and the nucleotide sequences of the genes coding for tRNA GCC (Gly) (trnG-GCC), tRNA GUC (Asp) (trnD-GUC) and tRNA GCA (Cys) (trnC-GCA) have been determined. These three genes are located in the large single copy region of the chloroplast genome, about half-way between one...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418249
更新日期:1985-09-01 00:00:00
abstract::Tremendous efforts have been taken worldwide to develop genome-wide genetic stocks for rice functional genomic (FG) research since the rice genome was completely sequenced. To facilitate FG research of complex polygenic phenotypes in rice, we report the development of over 20,000 introgression lines (ILs) in three eli...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-8519-3
更新日期:2005-09-01 00:00:00
abstract::Alignment of transcripts and genome sequences yielded a set of alternatively spliced transcripts in four angiosperm genomes: three dicotyledon species Medicago truncatula (Medicago), Populus trichocarpa (poplar) and Arabidopsis thaliana (Arabidopsis), and the monocotyledon Oryzae sativa (rice). Intron retention was th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9334-4
更新日期:2008-07-01 00:00:00
abstract:KEY MESSAGE:This research is the first to demonstrate that OsSAUR45 is involved in plant growth though affecting auxin synthesis and transport by repressing OsYUCCA and OsPIN gene expression in rice. Small auxin-up RNAs (SAURs) comprise a large multigene family and are rapidly activated as part of the primary auxin res...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0595-7
更新日期:2017-05-01 00:00:00
abstract::We isolated and characterized different classes of transposable DNA elements in oil palm (Elaeis guineensis) plants grown from seed, and plants regenerated from tissue culture that show mantling, an abnormality leading to flower abortion. Using PCR assays, reverse transcriptase fragments belonging to LINE-like and gyp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1023942309092
更新日期:2003-05-01 00:00:00
abstract:KEY MESSAGE:This manuscript describes the functions of an Argonaute protein named AGO17 in rice. AGO17 is required for the development of rice reproductive tissues. Argonaute (AGO) proteins are a well-conserved multigene family of regulators mediating gene silencing across eukaryotes. Monocot plants have additional mem...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01071-2
更新日期:2021-01-01 00:00:00
abstract::Free Air [CO(2)] Enrichment (FACE) allows for plant growth under fully open-air conditions of elevated [CO(2)] at concentrations expected to be reached by mid-century. We used Arabidopsis thaliana ecotypes Col-0, Cvi-0, and WS to analyze changes in gene expression and metabolite profiles of plants grown in "SoyFACE" (...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9041-y
更新日期:2006-11-01 00:00:00