Functional characterization of four APETALA2-family genes (RAP2.6, RAP2.6L, DREB19 and DREB26) in Arabidopsis.

Abstract:

:APETALA2 (AP2) transcription factors (TFs) play very important roles in plant growth and development and in defense response. Here, we report functional characterization of four AP2 TF family genes [(RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340)] that were identified among NaCl inducible transcripts in abscisic acid responsive 17 (ABR17) transgenic Arabidopsis in our previous microarray analyses. DREB19 and DREB26 function as transactivators and localize in the nucleus. All four genes were abundant in early vegetative and flowering stages, although the magnitude of the expression varied. We observed tissue specific expression patterns for RAP2.6, RAP2.6L, DREB19 and DREB26 in flowers and other organs. RAP2.6 and RAP2.6L were responsive to stress hormones like jasmonic acid, salicylic acid, abscisic acid and ethylene in addition to salt and drought. DREB19 and DREB26 were less responsive to stress hormones, but the former was highly responsive to salt, heat and drought. Overexpression of RAP2.6 in Arabidopsis resulted in a dwarf phenotype with extensive secondary branching and small siliques, and DREB26 overexpression resulted in deformed plants. However, overexpression of RAP2.6L and DREB19 enhanced performance under salt and drought stresses without affecting phenotype. In summary, we have demonstrated that RAP2.6, RAP2.6L, DREB26 and DREB19 are transactivators, they exhibit tissue specific expression, and they participate in plant developmental processes as well as biotic and/or abiotic stress signaling. It is possible that the results from this study on these transcription factors, in particular RAP2.6L and DREB19, can be utilized in developing salt and drought tolerant plants in the future.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Krishnaswamy S,Verma S,Rahman MH,Kav NN

doi

10.1007/s11103-010-9711-7

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

107-27

issue

1-2

eissn

0167-4412

issn

1573-5028

journal_volume

75

pub_type

杂志文章
  • CaMADS1, a MADS box gene expressed in the carpel of hazelnut.

    abstract::Hazelnut (Corylus avellana L.) is a species of economic interest that shows a peculiar floral biology. Unlike most of the angiosperms, which produce ovules during floral development such that they are ready for pollen at anthesis, hazelnut ovary development is delayed and triggered by compatible pollination. In order ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006022524708

    authors: Rigola D,Pè ME,Fabrizio C,Mè G,Sari-Gorla M

    更新日期:1998-12-01 00:00:00

  • Molecular and structural characterization of barley vernalization genes.

    abstract::Vernalization, the requirement of a period of low temperature to induce transition from the vegetative to reproductive state, is an evolutionarily and economically important trait in the Triticeae. The genetic basis of vernalization in cultivated barley (Hordeum vulgare subsp. vulgare) can be defined using the two-loc...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-0351-2

    authors: von Zitzewitz J,Szucs P,Dubcovsky J,Yan L,Francia E,Pecchioni N,Casas A,Chen TH,Hayes PM,Skinner JS

    更新日期:2005-10-01 00:00:00

  • Organization and transcription of the class I phycoerythrin genes of the marine cyanobacterium Synechococcus sp. WH7803.

    abstract::The nucleotide sequences of the class I phycoerythrin (PE) alpha- and beta-subunit genes (cpeA and cpeB) from the marine cyanobacterium Synechococcus sp. WH7803 are reported. The cpeB gene is located upstream of cpeA with a separation of 56 nucleotides and the two genes are co-transcribed as a transcript of 1.3 kb, wi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00023564

    authors: Newman J,Mann NH,Carr NG

    更新日期:1994-02-01 00:00:00

  • The DNA-compacting protein DCP68 from soybean chloroplasts is ferredoxin:sulfite reductase and co-localizes with the organellar nucleoid.

    abstract::The multiple copies of the chloroplast genome (plastome) are condensed and organized into nucleoids by a set of proteins. One of these, the DNA-binding protein DCP68 from soybean, has previously been shown to compact DNA and to inhibit DNA synthesis in vitro. N-terminal amino acid analysis and the absorption spectrum ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1015500431421

    authors: Chi-Ham CL,Keaton MA,Cannon GC,Heinhorst S

    更新日期:2002-08-01 00:00:00

  • Cloning and characterization of a class II DNA photolyase from Chlamydomonas.

    abstract::Damage to DNA induced by ultraviolet light can be reversed by a blue light-dependent reaction catalyzed by enzymes called DNA photolyases. Chlamydomonas has been shown to have DNA photolyase activity in both the nucleus and the chloroplast. Here we report the cloning and sequencing of a gene, PHR2, from Chlamydomonas ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006279720960

    authors: Petersen JL,Lang DW,Small GD

    更新日期:1999-08-01 00:00:00

  • Isolation of a subfamily of genes for R2R3-MYB transcription factors showing up-regulated expression under nitrogen nutrient-limited conditions.

    abstract::Plant R2R3-MYB transcription factors are encoded by more than 100 copies of genes. In this study, we attempted to isolate some members of the R2R3-MYB superfamily involved in regulation of nitrogen fixation in legumes. A library of 300 recombinant plasmid clones containing the R2R3-MYB fragments of the superfamily was...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/B:PLAN.0000009296.91149.34

    authors: Miyake K,Ito T,Senda M,Ishikawa R,Harada T,Niizeki M,Akada S

    更新日期:2003-09-01 00:00:00

  • Ectopic expression of the Arabidopsis MINI ZINC FINGER1 and MIF3 genes induces shoot meristems on leaf margins.

    abstract::A leaf undergoes determinate growth from a primordium on flank of the shoot apical meristem. Several intrinsic pathways restrict meristematic activity in the leaf of Arabidopsis; however, other factors remain to be defined. We report here that the overexpression of MINI ZINC FINGER1 (MIF1) or MIF3 disrupted the leaf d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9768-y

    authors: Hu W,Feng B,Ma H

    更新日期:2011-05-01 00:00:00

  • Localization of AtROP4 and AtROP6 and interaction with the guanine nucleotide dissociation inhibitor AtRhoGDI1 from Arabidopsis.

    abstract::The small GTPases of the Rho family play a key role in actin cytoskeletal organization. In plants, a novel Rho subfamily, called ROP (Rho of plants), has been found. In Arabidopsis, 12 ROP GTPases have been identified which differ mainly at their C-termini. To test the localization of two members of this subfamily (At...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006341210147

    authors: Bischoff F,Vahlkamp L,Molendijk A,Palme K

    更新日期:2000-02-01 00:00:00

  • The 22 bp W1 element in the pea lectin promoter is necessary and, as a multimer, sufficient for high gene expression in tobacco seeds.

    abstract::The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019103

    authors: de Pater S,Pham K,Klitsie I,Kijne J

    更新日期:1996-11-01 00:00:00

  • Profiling the expression of genes controlling rice grain quality.

    abstract::Rice provides a staple source of energy, protein and other nutrients to half of the world population. Over 90 of the rice seeds consists of starch and protein by dry weight. The quantity and property of starch and protein thus play a dominant role in the yield and quality of rice. The amylase content of starch is a de...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-7507-3

    authors: Duan M,Sun SS

    更新日期:2005-09-01 00:00:00

  • Tumor targeting of humanized fragment antibody secreted from transgenic rice cell suspension culture.

    abstract::The tumor-associated glycoprotein 72 (TAG 72) has been shown to be expressed in the majority of human adenocarcinomas. In an effort to develop a technique for the safe and inexpensive production of large quantities of anti-TAG 72 humanized antibody fragments (hzAb) as a future source of clinical-grade proteins, we dev...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9379-4

    authors: Hong SY,Lee TS,Kim J,Jung JH,Choi CW,Kim TG,Kwon TH,Jang YS,Yang MS

    更新日期:2008-11-01 00:00:00

  • Structure and expression of a cDNA encoding a histone H2A from Euglena gracilis.

    abstract::Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a pol...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00014447

    authors: Saint-Guily A,Schantz ML,Schantz R

    更新日期:1994-03-01 00:00:00

  • Normal and lysine-containing zeins are unstable in transgenic tobacco seeds.

    abstract::Chimeric genes composed of the beta-phaseolin promoter, an alpha-zein coding sequence and its modified versions containing lysine codons, and a beta-zein polyadenylation signal were inserted into the genome of tobacco by Agrobacterium-mediated transformation. alpha-Zein mRNA levels in the transgenic tobacco seeds 20 d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017922

    authors: Ohtani T,Galili G,Wallace JC,Thompson GA,Larkins BA

    更新日期:1991-01-01 00:00:00

  • Tetracycline-dependent activation of an upstream promoter reveals transcriptional interference between tandem genes within T-DNA in tomato.

    abstract::Tomato plants stably expressing the tetR gene were transformed with the beta-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005888616748

    authors: Thompson AJ,Myatt SC

    更新日期:1997-07-01 00:00:00

  • Over-expression of a scopoletin glucosyltransferase in Nicotiana tabacum leads to precocious lesion formation during the hypersensitive response to tobacco mosaic virus but does not affect virus resistance.

    abstract::Nicotiana tabacum Togt encodes a scopoletin glucosyltransferase (UDPglucose:scopoletin O -beta-D-glucosyltrans- ferase, EC 2.4.1.128) known to act in vitro on many different substrates including the 6-methoxy-7-hydroxy- coumarin scopoletin. This phenolic compound accumulates in vast amounts, essentially in its glucosy...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/B:PLAN.0000028775.58537.fe

    authors: Gachon C,Baltz R,Saindrenan P

    更新日期:2004-01-01 00:00:00

  • Overexpression of HbMBF1a, encoding multiprotein bridging factor 1 from the halophyte Hordeum brevisubulatum, confers salinity tolerance and ABA insensitivity to transgenic Arabidopsis thaliana.

    abstract:KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00926-7

    authors: Zhang L,Wang Y,Zhang Q,Jiang Y,Zhang H,Li R

    更新日期:2020-01-01 00:00:00

  • mRNA transcription profile of potato (Solanum tuberosum L.) exposed to ultrasound during different stages of in vitro plantlet development.

    abstract:KEY MESSAGE:In response to an ultrasound pulse, several hundred DEGs, including in response to stress, were up- or down-regulated in in vitro potato plantlets. Despite this abiotic stress, plantlets survived. Ultrasound (US) can influence plant growth and development. To better understand the genetic mechanism underlyi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00876-0

    authors: Dobránszki J,Hidvégi N,Gulyás A,Teixeira da Silva JA

    更新日期:2019-07-01 00:00:00

  • Plant hormones and nutrient signaling.

    abstract::Plants count on a wide variety of metabolic, physiological, and developmental responses to adapt their growth to variations in mineral nutrient availability. To react to such variations plants have evolved complex sensing and signaling mechanisms that allow them to monitor the external and internal concentration of ea...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1007/s11103-008-9380-y

    authors: Rubio V,Bustos R,Irigoyen ML,Cardona-López X,Rojas-Triana M,Paz-Ares J

    更新日期:2009-03-01 00:00:00

  • PCNA protein associates to Cdk-A type protein kinases in germinating maize.

    abstract::In higher eukaryotes, the proliferating cell nuclear antigen (PCNA) can be found associated to Cyclin D and Cdk4/6, the kinase complex responsible for cell cycle commitment in response to growth and mitogenic signals. During maize germination, PCNA can be found in protein complexes between 131 and 163 kDa. The sizes o...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1016029001537

    authors: de la Paz Sánchez M,Torres A,Boniotti MB,Gutierrez C,Vázquez-Ramo JM

    更新日期:2002-09-01 00:00:00

  • An in silico strategy identified the target gene candidates regulated by dehydration responsive element binding proteins (DREBs) in Arabidopsis genome.

    abstract::Identification of downstream target genes of stress-relating transcription factors (TFs) is desirable in understanding cellular responses to various environmental stimuli. However, this has long been a difficult work for both experimental and computational practices. In this research, we presented a novel computationa...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9414-5

    authors: Wang S,Yang S,Yin Y,Guo X,Wang S,Hao D

    更新日期:2009-01-01 00:00:00

  • Identification of early senescence-associated genes in rice flag leaves.

    abstract::Leaf senescence is one of the key stages of plant leaf development. It is a highly complex but ordered process involving expression of large scale senescence associated genes, and its molecular mechanisms still remain unclear. By using suppression subtractive hybridization, 815 ESTs that are up-regulated at the onset ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9300-1

    authors: Liu L,Zhou Y,Zhou G,Ye R,Zhao L,Li X,Lin Y

    更新日期:2008-05-01 00:00:00

  • Association of decreased expression of a Myb transcription factor with the TPD (tapping panel dryness) syndrome in Hevea brasiliensis.

    abstract::TPD (tapping panel dryness) is a complex physiological syndrome widely found in rubber tree (Hevea brasiliensis) plantations, which causes severe yield and crop losses in natural rubber-producing countries. The molecular mechanism underlying TPD is not known and there is presently no effective prevention or treatment ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1020719420867

    authors: Chen S,Peng S,Huang G,Wu K,Fu X,Chen Z

    更新日期:2003-01-01 00:00:00

  • Transformation of carrot tissues derived from proembryogenic suspension cells: A useful model system for gene expression studies in plants.

    abstract::A method is described for the high frequency transformation of carrot proembryogenic suspension culture cells by a non-oncogenic Ti-plasmid vector (pGV3850::1103) which carried a chimaeric kanamycin resistance gene (nos-NPT-II). Plants were regenerated efficiently from transformed material by somatic embryogenesis in ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00015034

    authors: Scott RJ,Draper J

    更新日期:1987-05-01 00:00:00

  • The precursor of the F1beta subunit of the ATP synthase is covalently modified upon binding to plant mitochondrial.

    abstract::We present evidence for a unique covalent modification of a nuclear-encoded precursor protein targeted to plant mitochondria. We investigated the early events of in vitro import for the mitochondrial precursor of the ATP synthase F1beta subunit from Nicotiana plumbaginifolia (pF1beta) into plant mitochondria. When pF1...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006375123496

    authors: von Stedingk E,Pavlov PF,Grinkevich VA,Glaser E

    更新日期:1999-11-01 00:00:00

  • Over-expression of a seed specific hevein-like antimicrobial peptide from Pharbitis nil enhances resistance to a fungal pathogen in transgenic tobacco plants.

    abstract::Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1019864222515

    authors: Koo JC,Chun HJ,Park HC,Kim MC,Koo YD,Koo SC,Ok HM,Park SJ,Lee SH,Yun DJ,Lim CO,Bahk JD,Lee SY,Cho MJ

    更新日期:2002-10-01 00:00:00

  • Identification and characterization of AtCASP, a plant transmembrane Golgi matrix protein.

    abstract::Golgins are a family of coiled-coil proteins that are associated with the Golgi apparatus. They are necessary for tethering events in membrane fusion and may act as structural support for Golgi cisternae. Here we report on the identification of an Arabidopsis golgin which is a homologue of CASP, a known transmembrane ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-4618-4

    authors: Renna L,Hanton SL,Stefano G,Bortolotti L,Misra V,Brandizzi F

    更新日期:2005-05-01 00:00:00

  • Two classes of differentially regulated glutamine synthetase genes are expressed in the soybean nodule: a nodule-specific class and a constitutively expressed class.

    abstract::We have characterized two sets of cDNA clones representing the glutamine synthetase (GS) mRNA in soybean nodules. Using the 3'-untranslated regions of a representative member of each set, as gene member(s) specific probes, we have shown that one set of the GS genes are expressed in a nodule-specific manner, while the ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00028970

    authors: Roche D,Temple SJ,Sengupta-Gopalan C

    更新日期:1993-09-01 00:00:00

  • Identification of cis-elements for ethylene and circadian regulation of the Solanum melongena gene encoding cysteine proteinase.

    abstract::We have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5'-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions an...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-0954-7

    authors: Rawat R,Xu ZF,Yao KM,Chye ML

    更新日期:2005-03-01 00:00:00

  • Expression characteristics of OS-ACS1 and OS-ACS2, two members of the 1-aminocyclopropane-1-carboxylate synthase gene family in rice (Oryza sativa L. cv. Habiganj Aman II) during partial submergence.

    abstract::Deepwater rice can grow in the regions of Southeast Asia that are flooded during the monsoon season because it has several adaptations allowing it to survive under flooded conditions. One such adaptation is the ability for rapid internode elongation upon partial submergence to maintain its foliage above the rising flo...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/b:plan.0000009693.26740.c3

    authors: Zarembinski TI,Theologis A

    更新日期:1997-01-01 00:00:00

  • The ltk gene family encodes novel receptor-like kinases with temporal expression in developing maize endosperm.

    abstract::We describe the isolation and characterization of maize cDNAs that are transcribed from a small gene family and encode a novel group of receptor-like kinases (RLKs). The distinctive extracellular domain of these novel RLKs includes a unique number and arrangement of leucine-rich repeats (LRRs), a proline-rich region (...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006012530241

    authors: Li Z,Wurtzel ET

    更新日期:1998-07-01 00:00:00