Abstract：
:The putative RNA helicase encoded by the Arabidopsis gene At1g32490 is a homolog of the yeast splicing RNA helicases Prp2 and Prp22. We isolated a temperature-sensitive allele (rsw12) of the gene in a screen for root radial swelling mutants. Plants containing this allele grown at the restrictive temperature showed weak radial swelling, were stunted with reduced root elongation, and contained reduced levels of cellulose. The role of the protein was further explored by microarray analysis. By using both fold change cutoffs and a weighted gene coexpression network analysis (WGCNA) to investigate coexpression of genes, we found that the radial swelling phenotype was not linked to genes usually associated with primary cell wall biosynthesis. Instead, the mutation has strong effects on expression of secondary cell wall related genes. Many genes potentially associated with secondary walls were present in the most significant WGCNA module, as were genes coding for arabinogalactans and proteins with GPI anchors. The proportion of up-regulated genes that possess introns in rsw12 was above that expected if splicing was unrelated to the activity of the RNA helicase, suggesting that the helicase does indeed play a role in splicing in Arabidopsis. The phenotype may be due to a change in the expression of one or more genes coding for cell wall proteins.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Howles PA,Gebbie LK,Collings DA,Varsani A,Broad RC,Ohms S,Birch RJ,Cork AH,Arioli T,Williamson REdoi
10.1007/s11103-016-0428-0subject
Has Abstractpub_date
2016-05-01 00:00:00pages
1-13issue
1-2eissn
0167-4412issn
1573-5028pii
10.1007/s11103-016-0428-0journal_volume
91pub_type
杂志文章abstract：:RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in maize mitochondria. The extent to which this molecule is associated with proteins was investigated by rate zonal and CsCl equilibrium density gradient centrifugation of clarified lysates of S cyt...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005818631401
更新日期：1997-06-01 00:00:00
abstract：:We have analysed the expression of the 8-10 members of the gene family encoding the flavonoid biosynthetic enzyme chalcone synthase (CHS) from Petunia hybrida. During normal plant development only two members of the gene family (CHS-A and CHS-J) are expressed. Their expression is restricted to floral tissues mainly. A...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020506
更新日期：1989-02-01 00:00:00
abstract：:Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of ligni...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-00991-3
更新日期：2020-06-01 00:00:00
abstract：:ATP-binding cassette transporters are involved in the active transport of a wide variety of metabolites in prokaryotes and eukaryotes. One subfamily, the Pleiotropic Drug Resistance (PDR) transporters, or full-size ABCG transporters, are found only in fungi and plants. NtPDR1 was originally identified in Nicotiana tab...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0053-0
更新日期：2013-05-01 00:00:00
abstract：:Infection of tobacco by tobacco mosaic virus (TMV) induces coordinate expression of genes encoding acidic and basic beta-1,3-glucanase isoforms. These genes are differentially expressed in response to other treatments. Salicylate treatment induces acidic glucanase mRNA to a higher level than basic glucanase mRNA. Ethy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028803
更新日期：1993-02-01 00:00:00
abstract：:The first step in the routing of newly synthesized proteins into the secretory pathway is the binding of the nascent signal sequence to the signal recognition particle. The mammalian signal recognition particle is a complex consisting of 6 proteins and a single 7S RNA molecule. Signal recognition particle-like complex...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00042359
更新日期：1993-12-01 00:00:00
abstract：:The PR-like proteins, class I beta-1,3-glucanase (GLU I) and chitinase (CHN I), are induced as part of a stereotypic response that can provide protection against viral, bacterial, and fungal pathogens. We have identified two Nicotiana plumbaginifolia ankyrin-repeat proteins, designated Glucanohydrolase Binding Protein...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-4679-9
更新日期：2004-11-01 00:00:00
abstract：:The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF02418241
更新日期：1985-07-01 00:00:00
abstract：:The role of the cell wall hydrolase polygalacturonase (PG) during fruit ripening was investigated using novel mutant tomato lines in which expression of the PG gene has been down regulated by antisense RNA. Tomato plants were transformed with chimaeric genes designed to express anti-PG RNA constitutively. Thirteen tra...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028773
更新日期：1990-03-01 00:00:00
abstract：KEY MESSAGE:Progression of the infection canal that conducts rhizobia to the nodule primordium requires a functional Rab GTPase located in Golgi/trans-Golgi that also participate in root hair polar growth. Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the format...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0581-5
更新日期：2017-04-01 00:00:00
abstract：:Alpha-dioxygenases (α-DOX) catalyzing the primary oxygenation of fatty acids to oxylipins were recently found in plants. Here, the biological roles of the pepper α-DOX (Ca-DOX) gene, which is strongly induced during non-host pathogen infection in chili pepper, were examined. Virus-induced gene silencing demonstrated t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0575-3
更新日期：2017-03-01 00:00:00
abstract：:Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF01578377
更新日期：1983-07-01 00:00:00
abstract：KEY MESSAGE:MdMYB16 forms homodimers and directly inhibits anthocyanin synthesis via its C-terminal EAR repressor. It weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis when overexpressing MdbHLH33 in callus overexpressing MdMYB16. MdMYB16 could interact with MdbHLH33. Anthocyanins are strong antioxidan...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-017-0601-0
更新日期：2017-05-01 00:00:00
abstract：:A certain nucleotide sequence in the promoter region of Vicia faba rRNA genes that specifically binds to a nuclear protein fraction has been identified by using a gel retardation assay and DNase I footprinting technique. The binding site of this protein fraction is located about 60 bp upstream from the initiation site...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027164
更新日期：1992-12-01 00:00:00
abstract：:RNA editing of T-urf13, a gene specific to the mitochondria of cytoplasmic male-sterile, type-T (cms-T) maize, and an adjacent, cotranscribed gene orf221, have been studied by cDNA sequencing. No editing was detected in 22 cDNA clones. This is the only report of a polypeptide-encoding gene in higher-plant mitochondria...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037148
更新日期：1991-11-01 00:00:00
abstract：:We present evidence for a unique covalent modification of a nuclear-encoded precursor protein targeted to plant mitochondria. We investigated the early events of in vitro import for the mitochondrial precursor of the ATP synthase F1beta subunit from Nicotiana plumbaginifolia (pF1beta) into plant mitochondria. When pF1...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006375123496
更新日期：1999-11-01 00:00:00
abstract：:Primer extension was used to characterize alpha-amylase mRNAs from aleurone tissue of barley (Hordeum vulgare L. cv. Himalaya) grains. Two synthetic oligonucleotides, specific for the low-pI and high-pI alpha-amylase groups, were used as primers for synthesis of cDNA from total RNA preparations. Between them, these tw...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023427
更新日期：1991-04-01 00:00:00
abstract：:Maize and tomato cDNA clones have been hybridized in Southern blotting experiments to plant genomic DNA prepared from different lines to detect restriction fragment polymorphisms (RFPs). In maize we have found that a high degree of genetic variability is present, even among domestic inbred lines. Most randomly chosen ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020093
更新日期：1985-03-01 00:00:00
abstract：KEY MESSAGE:OsWRKY36 represses plant height and grain size by inhibiting gibberellin signaling. Plant height and grain size are important agronomic traits affecting yield in cereals, including rice. Gibberellins (GAs) are plant hormones that promote plant growth and developmental processions such as stem elongation and...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01049-0
更新日期：2020-11-01 00:00:00
abstract：:A CDNA encoding a 47 kDa nucleoside triphosphatase (NTPase) that is associated with the chromatin of pea nuclei has been cloned and sequenced. The translated sequence of the cDNA includes several domains predicted by known biochemical properties of the enzyme, including five motifs characteristic of the ATP-binding do...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017808
更新日期：1996-01-01 00:00:00
abstract：:The conserved protein CBF5, initially regarded as a centromere binding protein in yeast and higher plants, was later found within nucleoli and in Cajal bodies of yeast and metazoa. There, it is assumed to be involved in posttranscriptional pseudouridinylation of various RNA species that might be important for RNA proc...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9226-z
更新日期：2007-11-01 00:00:00
abstract：:SAUR-AC1 is a small-auxin-up-RNA (SAUR) gene of Arabidopsis. Here we demonstrate that the SAUR-AC1 promoter region induces accumulation of a reporter transcript upon treatment with auxin and is preferentially active in elongating hypocotyls and certain other organs and tissues of transgenic Arabidopsis and tobacco pla...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005875300606
更新日期：1997-07-01 00:00:00
abstract：:Many chloroplast proteins are synthesized in the cytoplasm as precursors which contain an amino terminal transit peptide. These precursors are subsequently imported into chloroplast and targeted to one of several organellar locations. This import is mediated by the transit peptide, which is cleaved off during import. ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014912
更新日期：1987-07-01 00:00:00
abstract：KEY MESSAGE:CmCAD2 and CmCAD3 function more positively than CmCAD1 in oriental melon for lignin synthesis which is important to ensure internal water status and thus for drought tolerance. Well-lignification may be the guarantee of efficient axial water transport and barrier of lateral water flow in oriental melon tole...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01018-7
更新日期：2020-08-01 00:00:00
abstract：:In higher eukaryotes, the proliferating cell nuclear antigen (PCNA) can be found associated to Cyclin D and Cdk4/6, the kinase complex responsible for cell cycle commitment in response to growth and mitogenic signals. During maize germination, PCNA can be found in protein complexes between 131 and 163 kDa. The sizes o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1016029001537
更新日期：2002-09-01 00:00:00
abstract：:Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1013644026132
更新日期：2001-12-01 00:00:00
abstract：:Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function. Although only a few or no elements may be active within a genome at any time in any individual, the genomic alterations they cause can have major outcomes for a species. All major eleme...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:Considerable progress in tomato molecular biology has been made over the past five years. At least 19 different mRNAs which increase in amount during tomato fruit ripening have been cloned and genes for enzymes involved in cell wall degradation (polygalacturonase and pectinesterase) and ethylene synthesis (ACC synthas...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/BF00015607
更新日期：1992-05-01 00:00:00
abstract：:Analysis of genes controlling flowering time (heading date) contributes to our understanding of fundamental principles of plant development and is of practical importance because of the effects of flowering time on plant adaptation and crop yield. This review discusses the extent to which plants may share common genet...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：
更新日期：1997-09-01 00:00:00
abstract：:Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0249-y
更新日期：2014-12-01 00:00:00
