Abstract:
:We report the isolation of the second member, kin2, of a family of two cold-inducible genes of Arabidopsis thaliana. The proteins corresponding to the two genes have similarities to the small antifreeze proteins from Winter flounder. Kin1 and kin2 are organized in a close tandem array in the genome of A. thaliana. Both have three exons separated by introns with approximately the same length and location. The coding regions are highly conserved while the introns and especially the 3' flanking sequences of the mRNAs have diverged. The kin1 and kin2 genes are coordinately regulated in the cold. Unlike kin1, the kin2 mRNA has a detectable basal level, and accumulates to a higher level during acclimation. Both mRNAs are induced by 10 microM ABA but only kin2 responds strongly to drought and salinity stresses.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Kurkela S,Borg-Franck Mdoi
10.1007/BF00026794subject
Has Abstractpub_date
1992-07-01 00:00:00pages
689-92issue
4eissn
0167-4412issn
1573-5028journal_volume
19pub_type
杂志文章abstract::A cDNA clone encoding Arabidopsis thaliana galactokinase was fortuitously isolated during the course of a screen for plant homologues of a Saccharomyces cerevisiae peroxisome assembly gene, PAS9. Clones were sought which restored the ability of pas9 cells to grow on oleate as a sole carbon source, as oleate metabolism...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005816104743
更新日期:1997-06-01 00:00:00
abstract::Repetitive DNA sequences were detected directly on somatic metaphase chromosome spreads from soybean root tips using fluorescent in situ hybridization. Methods to spread the forty small metaphase chromosomes substantially free of cellular material were developed using protoplasts. The specific DNA probe was a 1.05 kb ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00036810
更新日期:1991-07-01 00:00:00
abstract::Cyclin-dependent protein kinases are involved in many crucial cellular processes and aspects of plant growth and development, but their precise roles in abiotic stress responses are largely unknown. Here, Arabidopsis thaliana CYCLIN-DEPENDENT KINASE G2 (CDKG2) was shown to act as a negative regulator of the salinity s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0324-z
更新日期:2015-06-01 00:00:00
abstract::We have sequenced and characterized the complete psbB gene cluster of Chlamydomonas reinhardtii chloroplast DNA. Although the petB and petD genes are located elsewhere, the sequential order of psbB, ORF31, psbN and psbH is identical to that of the psbB operon in higher plants. Also, intergenic non-coding regions are m...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00047405
更新日期:1993-07-01 00:00:00
abstract::Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019506
更新日期:1994-12-01 00:00:00
abstract::Leafy spurge (Euphorbia esula) is an herbaceous perennial weed that produces vegetatively from an abundance of underground adventitious buds. In this study, we report the effects of different environmental conditions on vegetative production and flowering competence, and determine molecular mechanisms associated with ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9621-8
更新日期:2010-05-01 00:00:00
abstract::Analysis of the structure of chromatin in cereal species using micrococcal nuclease (MNase) cleavage showed nucleosomal organization and a ladder with typical nucleosomal spacing of 175-185 bp. Probing with a set of DNA probes localized in the authentic telomeres, subtelomeric regions and bulk chromatin revealed that ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005912822671
更新日期:1998-01-01 00:00:00
abstract::The DNA sequence composition of 526 dicot and 345 monocot intron sequences have been characterized using computational methods. Splice site information content and bulk intron and exon dinucleotide composition were determined. Positions 4 and 5 of 5' splice sites contain different statistically significant levels of i...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00040537
更新日期:1992-09-01 00:00:00
abstract::Cistus creticus subsp. creticus is a plant of intrinsic scientific interest due to the distinctive pharmaceutical properties of its resin. Labdane-type diterpenes, the main constituents of the resin, exhibit considerable antibacterial and cytotoxic activities. In this study chemical analysis of isolated trichomes from...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9399-0
更新日期:2008-12-01 00:00:00
abstract::The transcript level and in turn protein density of the K(+)-uptake channel ZMK1 in maize (Zea mays) coleoptiles is controlled by the phytohormone auxin. ZMK1 is involved in auxin-regulated coleoptile elongation as well as gravi- and phototropism. To provide unequivocal evidence for the role of ZMK1 in these elementar...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-0047-2
更新日期:2006-07-01 00:00:00
abstract::We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005708611020
更新日期:1997-04-01 00:00:00
abstract::Plant cells respond to cold stress via a regulatory mechanism leading to enhanced cold acclimation accompanied by growth retardation. The C-repeat binding factor (CBF) signaling pathway is essential for cold response of flowering plants. Our previously study documented a novel CBF-like gene from the cold-tolerant Caps...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0181-1
更新日期:2014-06-01 00:00:00
abstract::The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0233-6
更新日期:2014-11-01 00:00:00
abstract::Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9428-z
更新日期:2009-02-01 00:00:00
abstract::The photosynthetic apparatus of plant chloroplasts contains two photosystems, termed Photosystem I (PSI) and Photosystem II (PSII). Both PSI and PSII contain several types of chlorophyll a/b-binding (CAB) polypeptides, at least some of which are structurally related. It has been previously shown that multiple genes en...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015643
更新日期:1987-03-01 00:00:00
abstract::Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9297-5
更新日期:2008-04-01 00:00:00
abstract::Mitogen-activated protein kinases (MAPKs) constitute one of the most critical signaling components in plants. A typical example is wound-induced protein kinase (WIPK), which functions during pathogen responses in tobacco plants (Nicotiana tabacum). Searching for direct down-stream components, we previously isolated a ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9240-1
更新日期:2007-12-01 00:00:00
abstract::The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040716
更新日期:1996-08-01 00:00:00
abstract::14-3-3 proteins function as regulators of a wide range of target proteins in all eukaryotes by effecting direct protein-protein interactions. Primarily, interactions between 14-3-3 proteins and their targets are mediated by phosphorylation at specific sites on the target protein. Hence, interactions with 14-3-3s are s...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1021261614491
更新日期:2002-12-01 00:00:00
abstract::β-conglycinin, a major seed protein in soybean, is composed of α, α', and β subunits sharing a high homology among them. Despite its many health benefits, β-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the α, α',...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9865-y
更新日期:2012-02-01 00:00:00
abstract::In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0640-6
更新日期:2017-09-01 00:00:00
abstract::The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension anal...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039617
更新日期:1993-01-01 00:00:00
abstract::In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0397-8
更新日期:2016-01-01 00:00:00
abstract::cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in lambda gt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino ac...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040617
更新日期:1992-11-01 00:00:00
abstract::GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thalian...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9345-1
更新日期:2008-08-01 00:00:00
abstract::Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of ligni...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-00991-3
更新日期:2020-06-01 00:00:00
abstract::The giant knotweed Fallopia sachalinensis (Polygonaceae) synthesizes phenylacetonitrile (PAN) from L-phenylalanine when infested by the Japanese beetle Popillia japonica or treated with methyl jasmonate (MeJA). Here we identified (E/Z)-phenylacetaldoxime (PAOx) as the biosynthetic precursor of PAN and identified a cyt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0459-6
更新日期:2016-06-01 00:00:00
abstract::In angiosperms the interactions between the secretory matrix of the stylar transmitting tract and the growing pollen tubes have central roles in determining a successful fertilization. Sp41 is a major glycosylated component of the soluble proteins of the transmitting tract matrix and exhibits (1-3)-beta-glucanase acti...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014970
更新日期:1995-12-01 00:00:00
abstract::The expression of a 25 kDa protein, encoded by the fused mitochondrial pcf gene, is associated with cytoplasmic male sterility (CMS) in petunia. To investigate the role of the 25 kDa protein in CMS we have transformed petunia and tobacco plants with constructs expressing a portion of the urfS sequence of the pcf cDNA ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042040
更新日期:1995-04-01 00:00:00
abstract::The regulation of genes in response to wounding is mediated in part by the octadecanoids 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA) and its methyl ester methyl jasmonate (MeJA). We identified, by differential display, an Arabidopsis gene (OPR3) induced after wounding. OPR3 is homologous to members of the flav...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006464822434
更新日期:2000-09-01 00:00:00