Abstract:
:A cDNA encoding CYP79B1 has been isolated from Sinapis alba. CYP79B1 from S. alba shows 54% sequence identity and 73% similarity to sorghum CYP79A1 and 95% sequence identity to the Arabidopsis T42902, assigned CYP79B2. The high identity and similarity to sorghum CYP79A1, which catalyses the conversion of tyrosine to p-hydroxyphenylacetaldoxime in the biosynthesis of the cyanogenic glucoside dhurrin, suggests that CYP79B1 similarly catalyses the conversion of amino acid(s) to aldoxime(s) in the biosynthesis of glucosinolates. Within the highly conserved 'PERF' and the heme-binding region of A-type cytochromes, the CYP79 family has unique substitutions that define the family-specific consensus sequences of FXP(E/D)RH and SFSTG(K/R)RGC(A/I)A, respectively. Sequence analysis of PCR products generated with CYP79B subfamily-specific primers identified CYP79B homologues in Tropaeolum majus, Carica papaya, Arabidopsis, Brassica napus and S. alba. The five glucosinolate-producing plants identified a CYP79B amino acid consensus sequence KPERHLNECSEVTLTENDLRFISFSTGKRGC. The unique substitutions in the 'PERF' and the heme-binding domain and the high sequence identity and similarity of CYP79B1, CYP79B2 and CYP79A1, together with the isolation of CYP79B homologues in the distantly related Tropaeolaceae, Caricaceae and Brassicaceae within the Capparales order, show that the initial part of the biosynthetic pathway of glucosinolates and cyanogenic glucosides is catalysed by evolutionarily conserved cytochromes P450. This confirms that the appearance of glucosinolates in Capparales is based on a cyanogen 'predisposition'. Identification of CYP79 homologues in glucosinolate-producing plants provides an important tool for tissue-specific regulation of the level of glucosinolates to improve nutritional value and pest resistance.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Bak S,Nielsen HL,Halkier BAdoi
10.1023/a:1006064202774subject
Has Abstractpub_date
1998-11-01 00:00:00pages
725-34issue
5eissn
0167-4412issn
1573-5028journal_volume
38pub_type
杂志文章abstract::The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such gen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020486
更新日期:1996-12-01 00:00:00
abstract::In this paper we present the structural analysis of the 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene family from Petunia hybrida. Southern blot analysis and restriction endonuclease mapping showed that two cloned regions of the petunia genome contained sequences highly homologous to a previously isolated ACC o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042349
更新日期:1993-12-01 00:00:00
abstract::Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could be detected only in Avicennia marina. Pinitol was the main osmoprotectant in the other three spec...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006497113323
更新日期:2001-02-01 00:00:00
abstract::Two different calmodulin (CaM) cDNAs (MBCaM-1 and MBCaM-2) were isolated from a vigna radiata lambda gt11 library by screening with a heterologous Arabidopsis cDNA probe (TCH-1). Both cDNAs are 85% homologous inside the coding region but are highly divergent outside this region. The polypeptides encoded by MBCaM-1 and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029857
更新日期:1994-03-01 00:00:00
abstract::Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00187572
更新日期:1983-01-01 00:00:00
abstract::The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040716
更新日期:1996-08-01 00:00:00
abstract::In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our identification revealed that the rice genome has 4 RAD21-like genes tha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-4922-z
更新日期:2006-03-01 00:00:00
abstract::The intrinsic chlorophyll-protein CP 47 is a component of photosystem II which functions in both light-harvesting and oxygen evolution. The large extrinsic loop E of this protein has been shown to interact with the oxygen-evolving site. Previously, Vermaas and coworkers have produced a number of deletions within loop ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041405
更新日期:1996-12-01 00:00:00
abstract:KEY MESSAGE:Plant bioregulators play an important role in managing oxidative stress tolerance in plants. Utilizing their ability in stress sensitive crops through genetic engineering will be a meaningful approach to manage food production under the threat of climate change. Exploitation of the plant defense system agai...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-020-01077-w
更新日期:2021-01-01 00:00:00
abstract::H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tiss...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028864
更新日期:1994-11-01 00:00:00
abstract::We have characterized the topoisomerase I and II activities in nuclear extracts from immature embryos of Zea mays and the effect of the treatment with 2,4-dichlorophenoxyacetic acid (2,4-D) and abscisic acid (ABA). These extracts were shown to be essentially devoid of protease and nuclease activities and they were tes...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017917
更新日期:1991-01-01 00:00:00
abstract::The PR-like proteins, class I beta-1,3-glucanase (GLU I) and chitinase (CHN I), are induced as part of a stereotypic response that can provide protection against viral, bacterial, and fungal pathogens. We have identified two Nicotiana plumbaginifolia ankyrin-repeat proteins, designated Glucanohydrolase Binding Protein...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-4679-9
更新日期:2004-11-01 00:00:00
abstract::To test the influence of a Nicotiana tomentosiformis repetitive sequence (R8.3) on transgene expression in N. sylvestris and in N. sylvestris-N. tomentosiformis hybrids, the R8.3 sequence was placed upstream of a nopaline synthase promoter (NOSpro)-NPTII reporter gene in a T-DNA construct. A number of transgenic N. sy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1023937006311
更新日期:2003-05-01 00:00:00
abstract::Arabidopsis is currently the reference genome for higher plants. A new, more detailed statistical analysis of Arabidopsis gene structure is presented including intron and exon lengths, intergenic distances, features of promoters, and variant 5'-ends of mRNAs transcribed from the same transcription unit. We also provid...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-2564-9
更新日期:2006-01-01 00:00:00
abstract::Plastidic acetyl-CoA carboxylase (ACCase) regulates the rate of fatty acid synthesis. This enzyme is composed of biotin carboxyl carrier protein (BCCP), biotin carboxylase (BC), and carboxyltransferase (CT), which consists of α and β subunits. Among these components, CTβ is encoded by the plastidic genome. In Arabidop...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0075-7
更新日期:2013-07-01 00:00:00
abstract::The cytokinin gene, isopentenyl transferase (ipt), was placed under the control of a heat-inducible promoter from the Drosophila melanogaster hsp70 gene and introduced into Nicotiana plumbaginifolia by cocultivation with Agrobacterium tumefaciens. Transformants were analyzed for organ-specific expression, cytokinin le...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017921
更新日期:1991-01-01 00:00:00
abstract::Seed dormancy is an important developmental process that prevents pre-harvest sprouting in many grains and other seeds. Abscisic acid (ABA), a plant hormone, plays a crucial role in regulating dormancy but the underlying molecular regulatory mechanisms are not fully understood. An Arabidopsis zinc-finger gene, MEDIATO...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000028730.10834.e3
更新日期:2004-01-01 00:00:00
abstract::Repetitive DNA sequences comprise a large percentage of plant genomes, and their characterization provides information about both species and genome evolution. We have isolated a recombinant clone containing a highly repeated DNA element (SB92) that is homologous to ca. 0.9% of the soybean genome or about 10(5) copies...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041174
更新日期:1995-11-01 00:00:00
abstract::A leaf cDNA library from hybrid aspen, Populus tremula x tremuloides, was constructed. From this two different cDNA clones, denoted CA1a and CA1b, encoding a chloroplastic carbonic anhydrase (CA) were isolated and DNA sequenced. Analysis of the deduced amino acid sequences showed that the isolated CAs belong to the be...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005849202731
更新日期:1997-07-01 00:00:00
abstract::Aquaporin belongs to a highly conserved group of membrane proteins called major intrinsic proteins that facilitate water transport across biological membranes. The genome of Arabidopsis encodes 35 aquaporin genes with 13 homologs in the plasma membrane intrinsic protein (PIP) subgroup. However, the function of each in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000040900.61345.a6
更新日期:2004-03-01 00:00:00
abstract::The gene coding for a new class of proteins rich in glycine and proline (GPRP) was cloned in Arabidopsis thaliana. In the protein sequence, five amino acids - glycine, proline, alanine, tyrosine and histidine - account for 79.4% of the total composition. The protein has two different glycine-rich domains interrupted b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00049336
更新日期:1996-02-01 00:00:00
abstract::The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006030007333
更新日期:1998-08-01 00:00:00
abstract::A cDNA library was made from poly(A)+ RNA isolated from developing Douglas fir (Pseudotsuga menziesii) embryo and megagametophytic tissue, and the cDNA clones were identified by immunoscreening with polyclonal antiserum against the crystalloid storage protein complex of Douglas fir. The nucleotide sequence of the long...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014555
更新日期:1993-02-01 00:00:00
abstract::The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00018558
更新日期:1990-02-01 00:00:00
abstract::Protein folding in vivo is mediated by an array of proteins that act either as 'foldases' or 'molecular chaperones'. Foldases include protein disulfide isomerase and peptidyl prolyl isomerase, which catalyze the rearrangement of disulfide bonds or isomerization of peptide bonds around Pro residues, respectively. Molec...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00039383
更新日期:1996-10-01 00:00:00
abstract:KEY MESSAGE:Salt stress triggers a simultaneous transcriptional repression and aquaporin internalization to modify root cell water conductivity. Plasma membrane intrinsic proteins (PIPs) are involved in the adjustment of plant water balance in response to changing environmental conditions. In this study, Arabidopsis wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0542-z
更新日期:2016-12-01 00:00:00
abstract::To understand the transcriptional responses to flooding stress in roots including hypocotyl of soybean seedlings, genome-wide changes in gene expression were analyzed using a soybean microarray chip containing 42,034 60-mer oligonucleotide probes. More than 6,000 of flooding-responsive genes in the roots including hyp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9799-4
更新日期:2011-09-01 00:00:00
abstract::We have isolated and analysed a 2 kb region of the mitochondrial genome of Arabidopsis thaliana (Columbia) showing a high level of nucleotide identity with the mitochondrial (mt) rps14 small-subunit ribosomal protein gene from Oenothera berteriana and Vicia faba, as well as with an open reading frame (ORF) located ups...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028903
更新日期:1992-12-01 00:00:00
abstract::Exposure of seedlings of a chilling-sensitive variety of rice (Oryza sativa L. cv. Wasetoittu) to water stress (0.5 M mannitol, 30 min) at room temperature induced a degree of chilling resistance. No such resistance was induced by exogenous abscisic acid (ABA) application (10 microM, 60 min). Upon short-term water str...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039544
更新日期:1994-10-01 00:00:00
abstract::Proline-rich protein (PRP), a cell wall protein of plant, has been studied in many plant species. Yet, none of the PRPs has been functionally elucidated. Here we report a novel flower-specific PRP designated OsPRP3 from rice. Expression analysis showed that the OsPRP3 transcript was mainly present in rice flower and a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9557-z
更新日期:2010-01-01 00:00:00