RNA-seq transcriptional profiling of Herbaspirillum seropedicae colonizing wheat (Triticum aestivum) roots.

Abstract:

:Herbaspirillum seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize plants, wheat seedlings growing hydroponically in Hoagland's medium were inoculated with H. seropedicae and incubated for 3 days. Total mRNA from the bacteria present in the root surface and in the plant medium were purified, depleted from rRNA and used for RNA-seq profiling. RT-qPCR analyses were conducted to confirm regulation of selected genes. Comparison of RNA profile of root attached and planktonic bacteria revealed extensive metabolic adaptations to the epiphytic life style. These adaptations include expression of specific adhesins and cell wall re-modeling to attach to the root. Additionally, the metabolism was adapted to the microxic environment and nitrogen-fixation genes were expressed. Polyhydroxybutyrate (PHB) synthesis was activated, and PHB granules were stored as observed by microscopy. Genes related to plant growth promotion, such as auxin production were expressed. Many ABC transporter genes were regulated in the bacteria attached to the roots. The results provide new insights into the adaptation of H. seropedicae to the interaction with the plant.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Pankievicz VC,Camilios-Neto D,Bonato P,Balsanelli E,Tadra-Sfeir MZ,Faoro H,Chubatsu LS,Donatti L,Wajnberg G,Passetti F,Monteiro RA,Pedrosa FO,Souza EM

doi

10.1007/s11103-016-0430-6

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

589-603

issue

6

eissn

0167-4412

issn

1573-5028

pii

10.1007/s11103-016-0430-6

journal_volume

90

pub_type

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