Abstract：
:Secretion, endocytosis and transport to the lytic compartment are fundamental, highly coordinated features of the eukaryotic cell. These intracellular transport processes are facilitated by vesicles, many of which are small (100 nm or less in diameter) and 'coated' on their cytoplasmic surface. Research into the structure of the coat proteins and how they interact with the components of the vesicle membrane to ensure the selective packaging of the cargo molecules and their correct targeting, has been quite extensive in mammalian and yeast cell biology. By contrast, our knowledge of the corresponding types of transport vesicles in plant cells is limited. Nevertheless, the available data indicate that a considerable homology between plant and non-plant coat polypeptides exists, and it is also suggestive of a certain similarity in the mechanisms underlying targeting in all eukaryotes. In this article we shall concentrate on three major types of transport vesicles: clathrin-coated vesicles, COP-coated vesicles, and 'dense' vesicles, the latter of which are responsible for the transport of vacuolar storage proteins in maturing legume cotyledons. For each we will summarize the current literature on animal and yeast cells, and then present the relevant data derived from work on plant cells. In addition, we briefly review the evidence in support of the 'SNARE' hypothesis, which explains how vesicles find and fuse with their target membrane.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Robinson DG,Hinz G,Holstein SEsubject
Has Abstractpub_date
1998-09-01 00:00:00pages
49-76issue
1-2eissn
0167-4412issn
1573-5028journal_volume
38pub_type
杂志文章,评审abstract：:The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked b...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00032673
更新日期：1995-09-01 00:00:00
abstract：:An important group of splicing factors involved in constitutive and alternative splicing contain an arginine/serine (RS)-rich domain. We have previously demonstrated the existence of such factors in plants and report now on a new family of splicing factors (termed the RSZ family) from Arabidopsis thaliana which additi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006129615846
更新日期：1999-03-01 00:00:00
abstract：:To analyse experimentally the correlation between transgene silencing and the presence of an inverted repeat in transgenic Arabidopsis thaliana plants, expression of the beta-glucuronidase (gus) gene was studied when present as a convergently transcribed inverted repeat or as a single copy in otherwise isogenic lines....
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1010614522706
更新日期：2001-07-01 00:00:00
abstract：:In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0244-3
更新日期：2014-11-01 00:00:00
abstract：:Nicotine biosynthesis in tobacco (Nicotiana tabacum L.) is highly regulated by jasmonic acid (JA). Two nuclear loci, A and B (renamed NIC1 and NIC2) have been identified that mediate JA-inducible nicotine formation and total alkaloid accumulation. NIC2 was recently shown to be a cluster of seven genes encoding Apetala...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0116-2
更新日期：2014-01-01 00:00:00
abstract：:We have analyzed the nucleotide sequence and accumulation of an mRNA which is prevalent in seeds of Brassica napus L. During normal development, the mRNA begins to accumulate during late embryogeny, is stored in dry seeds, and becomes undetectable in seedlings within 24 hours after imbibition. Moreover, abscisic acid ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017579
更新日期：1989-04-01 00:00:00
abstract：:Infection of tobacco by tobacco mosaic virus (TMV) induces coordinate expression of genes encoding acidic and basic beta-1,3-glucanase isoforms. These genes are differentially expressed in response to other treatments. Salicylate treatment induces acidic glucanase mRNA to a higher level than basic glucanase mRNA. Ethy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028803
更新日期：1993-02-01 00:00:00
abstract：:We previously identified a cluster of d ormancy-a ssociated M ADS-box transcription factors (DAM genes) in peach [Prunus persica (L.) Batsch] as potential candidates for control of the non-dormant phenotype observed in the evg mutant. Of these genes, DAM3, DAM5 and DAM6 were winter expressed, suggesting a role for the...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9608-5
更新日期：2010-05-01 00:00:00
abstract：:Abscisic acid (ABA) is one of the central plant hormones, responsible for controlling both maturation and germination in seeds, as well as mediating adaptive responses to desiccation, injury, and pathogen infection in vegetative tissues. Thorough analyses of two barley genes, HVA1 and HVA22, indicate that their respon...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9017-y
更新日期：2006-09-01 00:00:00
abstract：:Coordinated cell growth and differentiation is crucial for the development of higher plants. Using the elongation defective 1-1 (eld1-1) mutant, we cloned the ELD1 gene, which encodes a serine-rich protein. Genes homologous to ELD1 can be found in plants, including Arabidopsis, rice, and tobacco, but not in other orga...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/B:PLAN.0000019067.05185.d6
更新日期：2003-11-01 00:00:00
abstract：:Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD factors (UPF1, UPF2 ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9726-0
更新日期：2011-02-01 00:00:00
abstract：:In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expressi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028867
更新日期：1994-11-01 00:00:00
abstract：KEY MESSAGE:A novel open reading frame (ORF) identified and cloned from the A4 cytoplasm of Cajanus cajanifolius induced partial to complete male sterility when introduced into Arabidopsis and tobacco. Pigeonpea (Cajanus cajan L. Millsp.) is the only legume known to have commercial hybrid seed technology based on cytop...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-0728-7
更新日期：2018-05-01 00:00:00
abstract：:GALACTINOL SYNTHASE is the first committed enzyme in the raffinose biosynthetic pathway. We have previously characterized the maize (Zea mays) GALACTINOL SYNTHASE2 gene (ZmGOLS2) as abiotic stress induced. To further investigate the regulation of ZmGOLS2 gene expression, individual luciferase expression vectors,in whi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-015-0403-1
更新日期：2016-01-01 00:00:00
abstract：:We identified a novel nucleoplasm localized protein in Arabidopsis called AT-hook motif nuclear localized protein 1 (AHL1), which was isolated by visual screening of transformants using random GFP::cDNA fusions. AHL1 contains an AT-hook motif and unknown conserved PPC (plants and prokaryotes conserved) domain that inc...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-3249-5
更新日期：2004-09-01 00:00:00
abstract：:Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcriptio...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9752-6
更新日期：2011-04-01 00:00:00
abstract：:Using RNA from developing cotyledons and leaves of bean two products were synthesizedin vitro, with Mr 31000 and 31500 which are antigenically related to the subunit of phytoferritin (Mr 26500). The relative abundance of the two products is dependent on the type of tissue from which the RNA was derived. Evidence is pr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF01578592
更新日期：1983-11-01 00:00:00
abstract：:Homospermidine synthase is the first specific enzyme in the biosynthesis of pyrrolizidine alkaloids. Whereas the substrates putrescine and spermidine are part of the highly dynamic polyamine pool of plants, the product homospermidine is incorporated exclusively into the necine base moiety of pyrrolizidine alkaloids. R...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9514-x
更新日期：2009-09-01 00:00:00
abstract：:A cDNA clone (lp3) from loblolly pine induced by water deficit stress (WDS) has been isolated. It is preferentially induced in roots with a constitutive basal level of expression also observed in stems and needles. Northern blot analysis with well irrigated ABA-treated seedlings indicated that the overall accumulation...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005897921567
更新日期：1997-12-01 00:00:00
abstract：KEY MESSAGE:A Kelch repeat F-box containing protein, SMALL AND GLOSSY LEAVES1 (SAGL1) regulates phenylpropanoid biosynthesis as a post-translational regulator for PAL1 (phenylalanine ammonia-lyase) and an indirect transcriptional regulator for ANTHOCYANIDIN SYNTHASE. Phenylpropanoid biosynthesis in plants produces dive...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-0808-8
更新日期：2019-01-01 00:00:00
abstract：:We report the isolation of the second member, kin2, of a family of two cold-inducible genes of Arabidopsis thaliana. The proteins corresponding to the two genes have similarities to the small antifreeze proteins from Winter flounder. Kin1 and kin2 are organized in a close tandem array in the genome of A. thaliana. Bot...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00026794
更新日期：1992-07-01 00:00:00
abstract：:Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040527
更新日期：1992-09-01 00:00:00
abstract：:Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9297-5
更新日期：2008-04-01 00:00:00
abstract：KEY MESSAGE:Combining with a CRISPR/Cas9 system, Agrobacterium-mediated transformation can lead to precise targeted T-DNA integration in the rice genome. Agrobacterium-mediated T-DNA integration into the plant genomes is random, which often causes variable transgene expression and insertional mutagenesis. Because T-DNA...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-00819-1
更新日期：2019-03-01 00:00:00
abstract：:Two subtractive cDNA libraries were developed to study genes associated with bud dormancy (reverse library) and initiation of shoot growth (forward library) in leafy spurge. To identify unique sequences represented in each library, 15744 clones were screened to reduce the level of redundancy within both libraries. A t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-0015-x
更新日期：2006-05-01 00:00:00
abstract：:The transcription factor ABA-Insensitive5 (ABI5) is a key regulator of ABA signaling and stress response in Arabidopsis seeds and seedlings. Potential ABI5-interacting proteins were identified by a yeast two-hybrid screen; the most prevalent interactors were a family of four highly conserved plant-specific proteins wi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9344-2
更新日期：2008-08-01 00:00:00
abstract：:Hevea brasiliensis, being the only source of commercial natural rubber, is an extremely economically important crop. In an effort to facilitate biological, biochemical and molecular research in rubber biosynthesis, here we report the use of next-generation massively parallel sequencing technologies and de novo transcr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9811-z
更新日期：2011-10-01 00:00:00
abstract：:A mutant exhibiting conditional male sterility, in which fertility was restored under conditions of high humidity, was identified in T-DNA tagged lines of Arabidopsis thaliana. Scanning electron microscopy (SEM) demonstrated that the pollen surface was almost smooth and the reticulate pattern not prominent. Thus, the ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/B:PLAN.0000009269.97773.70
更新日期：2003-09-01 00:00:00
abstract：:An approximately 247-kb genomic region from FF genome of wild rice Oryza brachyantha, possessing the smallest Oryza genome, was compared to the orthologous approximately 450-kb region from AA genome, O. sativa L. ssp. japonica. 37 of 38 genes in the orthologous regions are shared between japonica and O. brachyantha. A...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9178-3
更新日期：2007-07-01 00:00:00
abstract：:The identification of some nodule-specific host proteins (nodulins) from common bean (Phaseolus vulgaris L.), a tropical ureide-transporting legume, is described. Particularly, the existence and developmental expression of several abundant nodule-specific transcripts of P. vulgaris are shown, including leghemoglobin, ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020530
更新日期：1987-11-01 00:00:00
