Oligonucleotide biological activity: relationship to the cell cycle and nuclear transport.

Abstract:

:Previous studies suggest that oligodeoxynucleotide (ODN) cellular uptake is cell cycle-dependent which may have important implications in cancer cell targeting. To further our understanding of ODN transport and activity, this study examines the relationships between the cell cycle, ODN cellular uptake, intracellular transport, and activity. An antisense c-myc ODN 21-mer was used to study ODN cellular uptake in Rauscher erythroleukemia cells synchronized by either chemical methods or flow cytometry. ODN uptake was examined using subcellular fractionation and confocal fluorescence microscopy. Western blot analysis was used to measure ODN-mediated decreases in c-myc protein levels. Intracellular ODN distribution and extent of uptake was influenced by the phase of the cell cycle, but the mechanism of uptake was not. The relative activity of the antisense ODN was positively correlated to ODN distribution to the cytosol, but negatively correlated to total cellular uptake. Although ODN total cellular uptake is positively influenced by the cell cycle, retention of the ODN in the cytosol (presumably extra-vesicularly) appeared to be relevant in determining the activity of an antisense ODN. Novel methods to target cytosol-acting drugs to the cytoplasm may therefore be warranted.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Wu-Pong S,Bard J,Huffman J,Jimerson J

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

257-61

issue

4

eissn

0248-4900

issn

1768-322X

pii

S0248-4900(97)82313-1

journal_volume

89

pub_type

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