Abstract：
:After gradually moving away from preparation methods prone to artefacts such as plastic embedding and negative staining for cell sections and single particles, the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of biological objects of various sizes. This 'revolution in resolution' is happening largely thanks to new developments of new-generation cameras used for recording the images in the cryo electron microscope which have much increased sensitivity being based on complementary metal oxide semiconductor devices. Combined with advanced image processing and 3D reconstruction, the cryo-EM analysis of nucleoprotein complexes can provide unprecedented insights at molecular and atomic levels and address regulatory mechanisms in the cell. These advances reinforce the integrative role of cryo-EM in synergy with other methods such as X-ray crystallography, fluorescence imaging or focussed-ion beam milling as exemplified here by some recent studies from our laboratory on ribosomes, viruses, chromatin and nuclear receptors. Such multi-scale and multi-resolution approaches allow integrating molecular and cellular levels when applied to purified or in situ macromolecular complexes, thus illustrating the trend of the field towards cellular structural biology.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Orlov I,Myasnikov AG,Andronov L,Natchiar SK,Khatter H,Beinsteiner B,Ménétret JF,Hazemann I,Mohideen K,Tazibt K,Tabaroni R,Kratzat H,Djabeur N,Bruxelles T,Raivoniaina F,Pompeo LD,Torchy M,Billas I,Urzhumtsev A,Klaholdoi
10.1111/boc.201600042subject
Has Abstractpub_date
2017-02-01 00:00:00pages
81-93issue
2eissn
0248-4900issn
1768-322Xjournal_volume
109pub_type
杂志文章,评审abstract：:A quantitative ultrastructural study was performed on 56 ejaculates showing anomalies of the sperm axonemal complex. The anomalies comprised either the absence of one, or more often several, axonemal structures, or defective elongation of the doublets. Several characteristics relating to the extent and superimposition...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00253.x
更新日期：1984-01-01 00:00:00
abstract：:Previous experiments have revealed a relatively weak electrostatic binding capacity of in vitro reconstituted intermediate filaments (IFs) as well as of natural IFs of whole cell mount preparations for purified ribosomal particles of mammalian origin. In order to demonstrate that such associations also occur in vivo, ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:Interactions of cells with the extracellular matrix are essential for the control of tissue remodelling, cell migration, and embryogenesis. At the cell-extracellular matrix contact points, specialized structures are formed and termed focal adhesions, where transmembrane adhesion receptors provide a structural link bet...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(00)01101-1
更新日期：2000-10-01 00:00:00
abstract：:Flow cytometry (FCM) analysis of cellular DNA content has become an increasingly important clinical research tool for the measurement and identification of abnormal cell populations. Examination of cellular DNA content can provide information for use in cell cycle analysis in cancer studies. Most researchers would agr...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/0248-4900(93)90116-v
更新日期：1993-01-01 00:00:00
abstract：:Alpha 2-macroglobulin (alpha 2M) is a plasma inhibitor of proteinases, the steric mechanism of which is based on a considerable conformational change. The typical and distinct H-like shape of alpha 2M-chymotrypsin (alpha 2M-chy) complexes seen by electron microscopy led us to an ultrastructural study of the binding of...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(88)90092-5
更新日期：1988-01-01 00:00:00
abstract：:A digitally-enhanced videomicroscopy study of rabbit gastric parietal cells in primary culture was performed using alternate observations with differential interference contrast and fluorescence optics of cells mounted and perfused on a temperature-controlled microscope stage. The effect of histamine, a physiological ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90349-8
更新日期：1990-01-01 00:00:00
abstract：:We have characterized the selectivity filter of the water channel aquaporin-1 (AQP1) of proximal straight tubules (PST), as an equivalent cylindrical structure with a diameter of approximately 4.5 A, where water molecules single file. We report here efforts to evaluate its length. PST were dissected from rabbit kidney...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
abstract：:Although there are many macromolecular complexes which play extremely important roles in biology, and despite continued progress in X-ray crystallographic and NMR methods, it is still very difficult to obtain atomic level structural information about such large assemblies. It is now clear that a powerful approach is t...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/j.1768-322x.1994.tb00932.x
更新日期：1994-01-01 00:00:00
abstract：:The activation of M-phase promoting factor (MPF) in one-cell mouse embryo is independent from the nucleus. Other autonomous phenomena include the cortical activity observed at the end of the first cell cycle and the reorganization of the microtubule network. Here, we observed that the autonomous control of MPF activat...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：BACKGROUND INFORMATION:There are several reports indicating that starved fibroblasts show higher proliferation rates when re-fed with foetal bovine serum. We have evidence demonstrating that this phenomenon is related to secretory proteins which may be beneficial to wound healing. RESULTS:After re-feeding, 16 and 72 h...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201300063
更新日期：2014-05-01 00:00:00
abstract：:The water permeability of collecting ducts is greatly increased by the antidiuretic hormone, vasopressin (VP). Freeze-fracture studies were carried out to test if this permeability increase is associated with the appearance of intramembrane particle (IMP) aggregates and whether increased doses of VP lead to an increas...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The dermis of the frog skin (Rana esculenta) displayed a remarkable organization of vertical and horizontal tracts. Vertical thick tracts connected the dermal Stratum spongiosum with the subcutaneous tissue. Horizontal thin tracts were found alongside and contiguous to them. The thick tracts were sheathed by collagen ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00533.x
更新日期：1987-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Matrix metalloproteinases (MMPs) are the key enzymes responsible for the remodelling of extracellular matrix. Two of them, namely MMP-2 and MMP-9 (gelatinases A and B, respectively), are expressed in skeletal muscles and are involved in their regeneration after the injury. Although MMPs are prima...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201300020
更新日期：2013-08-01 00:00:00
abstract：:In order to determine whether the human lactotransferrin receptor recently described on platelets was also present on hematopoietic precursors, we investigated its presence and characteristics on the megakaryocytic Dami cell line. The reversible binding of human 5-(([2-(carbo(hydrazino)methyl]thio)acetyl)aminofluoresc...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(94)80017-0
更新日期：1994-01-01 00:00:00
abstract：:We have recently shown that staurosporine (ST) can trigger apoptosis of CaSki and HeLa cervical tumor cells from G2/M checkpoint, though the mechanism remains elusive. In this study, we reported that ST induced the inhibition of E6 and E7 viral oncogene and MDM2 expression, while it led to increased levels of p53, whi...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(02)01220-0
更新日期：2003-01-01 00:00:00
abstract：:Netrins are secreted proteins that play a crucial role in neuronal migration and in axon guidance during the development of the nervous system. Netrin-1 has been shown to interact with the transmembrane receptors DCC and UNC5H and these receptors appeared of key importance in mediating the chemotropic activity of netr...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(03)00072-8
更新日期：2003-10-01 00:00:00
abstract：BACKGROUND INFORMATION:CLEM (correlative live cell and electron microscopy) seeks to bridge the data acquired with different imaging strategies, typically between light microscopy and electron microscopy. It has been successfully applied in cell cultures, although its use in multicellular systems is hampered by difficu...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20090096
更新日期：2009-12-04 00:00:00
abstract：BACKGROUND INFORMATION:In a previous study, we demonstrated that human neutrophils can develop membrane tubulovesicular extensions (TVEs) that are 160-250 nm in width and several micrometres long. These extensions, or cytonemes, are capable of establishing long-range contacts with other cells or bacteria. Cytonemes con...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201400063
更新日期：2015-05-01 00:00:00
abstract：:This study was undertaken to evaluate whether hypertrophy and hyperplasia of the pancreatic acinar cells induced by caerulein remained after termination of the hormonal treatment. Rats received subcutaneous injections of saline or caerulein for 4 days and were killed immediately after termination of treatment or 2, 15...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Genetic studies in the budding yeast have led to the molecular characterization of gamma-tubulin associated proteins and to the identification of orthologues in animal cells. While the gamma-tubulin complex is more complex in animal cells than in budding yeast, its function is probably maintained throughout evolution....
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1999-05-01 00:00:00
abstract：BACKGROUND INFORMATION:Autofluorescence spectroscopy is a powerful tool for molecular histology and for following metabolic processes in biological samples as it does not require labelling. However, at the microscopic scale, it is mostly limited to visible and near infrared excitation of the samples. Several interestin...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201200075
更新日期：2013-07-01 00:00:00
abstract：BACKGROUND INFORMATION:Nucleolin is a major nucleolar protein which is highly expressed in rapidly dividing cells and cancer cell lines. This protein is claimed to be multifunctional and could play a role in rRNA (ribosomal RNA) synthesis, as well as in cell division or response to cellular stresses. Therefore, how nuc...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20080054
更新日期：2009-03-01 00:00:00
abstract：BACKGROUND INFORMATION:MTA1 (metastasis-associated gene 1) has been reported to be overexpressed in cancers with high potential to metastasize. Studies of the molecular mechanisms revealed that MTA1 plays an important role in the process of metastasis of many types of cancer. However, the role of MTA1 in melanoma devel...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/bc20060130
更新日期：2007-10-01 00:00:00
abstract：:Extensive developmentally programmed DNA rearrangements, including thousands of internal deletions, occur in the differentiating somatic macronucleus in Tetrahymena thermophila. Some deletion systems involve the use of multiple alternative deletion sites. We report here the cloning and the sequences of three new alter...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(96)84773-3
更新日期：1996-01-01 00:00:00
abstract：:Detection of nucleic acid sequence at the ultrastructural level has allowed us to better understand the expression of genes in some fields of application in cell biology. In situ hybridization at the ultrastructural level can be carried out using three different methods: on vibratome sections before embedding in epoxy...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1998-07-01 00:00:00
abstract：:In the present study, we describe the structural and cytological changes observed in staggerer mutant olfactory bulbs, as compared to normal mice. On the basis of photonic and ultrastructural observations we tried to define the alterations induced by the mutation: i.e. a reduction of bulb size, a reduction in the volu...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Phosphorylation of the transcription factor CREB appears as an important step in the signal transduction cascade that activates melatonin biosynthesis in the mammalian pineal organ. We have studied the mechanisms causing CREB phosphorylation by immunocytochemical and immunochemical demonstration of phosphorylated CREB...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(98)80006-3
更新日期：1997-11-01 00:00:00
abstract：:The PAS-positive-calcium-sensitive (Ca-s) cells of the pars intermedia (PI) were studied in goldfish kept in fresh water (FW), deionized water (DW), 1/3 sea water (SW) and 1/3 Ca-free SW. Ultrastructural studies show that Ca-s cells of control goldfish kept in FW have a low activity with elongated or deeply indented n...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00202.x
更新日期：1983-01-01 00:00:00
abstract：:A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human ce...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00539.x
更新日期：1987-01-01 00:00:00
abstract：:PD (peritoneal dialysis) is an established mode of renal replacement therapy, based on the exchange of fluid and solutes between blood in peritoneal capillaries and a dialysate that has been introduced into the peritoneal cavity. The dialysis process involves diffusive and convective transports and osmosis through the...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20040132
更新日期：2005-09-01 00:00:00
