Abstract：
:The dermis of the frog skin (Rana esculenta) displayed a remarkable organization of vertical and horizontal tracts. Vertical thick tracts connected the dermal Stratum spongiosum with the subcutaneous tissue. Horizontal thin tracts were found alongside and contiguous to them. The thick tracts were sheathed by collagen fibrils of the Stratum compactum which were vertically oriented (i.e. parallel to the axes of the tracts) according to the horizontal and orthogonal arrangement of the collagen bundles of the Stratum compactum. The thin tracts devoid of collagenous sheath were formed by clear spaces between superimposed collagen bundles of the dermal Stratum compactum. On vertical sections, the thick tracts were seen to contain fibronectin (FN), detected by indirect immunoperoxidase. Continuous vertical FN lines were centred in these tracts. On horizontal sections, a clear zone around these FN-centred lines was also sheathed by FN. The thick tracts contained flattened pigmentary cells and fibroblasts; these cells were FN-outlined. The thin tracts contained patches of FN and FN-outlined fibroblasts. In culture, in vertical thick tracts, both pigmentary cells and fibroblasts disappeared when antiserum to FN was added to the culture medium. This suggested that thick tracts were pathways allowing pigmentary cells to move upward or downward between their usual upper dermal and lower subcutaneous localizations. Fewer fibroblasts were found in the thin tracts in the presence of antiserum to FN.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Denèfle JP,Zhu QL,Lechaire JPdoi
10.1111/j.1768-322x.1987.tb00533.xsubject
Has Abstractpub_date
1987-01-01 00:00:00pages
219-26issue
3eissn
0248-4900issn
1768-322Xjournal_volume
59pub_type
杂志文章abstract：:Flow cytometry has important advantages over conventional techniques. It is rapid, highly sensitive and allows multi-parametric analysis and cell sorting. Potential exists for the measurement of many cell functions by flow cytometry. The technique can be used to determine cell viability, intracellular calcium and pH, ...
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journal_title：Biology of the cell
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doi：10.1111/j.1768-322x.1986.tb00512.x
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journal_title：Biology of the cell
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更新日期：1984-01-01 00:00:00
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更新日期：1999-05-01 00:00:00
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