Possible role of cortactin phosphorylation by protein kinase Cα in actin-bundle formation at growth cone.

Abstract:

BACKGROUND INFORMATION:Cortactin contributes to growth cone morphogenesis by forming with dynamin, ring-shaped complexes that mechanically bundle and stabilise F-actin. However, the regulatory mechanism of cortactin action is poorly understood. RESULTS:Immunofluorescence microscopy revealed that protein kinase C (PKC) α colocalises with cortactin at growth cone filopodia in SH-SY5Y neuroblastoma cells. PKC activation by phorbol 12-myristate 13-acetate causes cortactin phosphorylation, filopodial retraction and F-actin-bundle loss. Moreover, PKCα directly phosphorylates cortactin in vitro at S135/T145/S172, mitigating both cortactin's actin-binding and actin-crosslinking activity, whereas cellular expression of a phosphorylation-mimetic cortactin mutant hinders filopodial formation with a significant decrease of actin bundles. CONCLUSIONS:Our results indicate that PKC-mediated cortactin phosphorylation might be implicated in the maintenance of growth cone.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Yamada H,Kikuchi T,Masumoto T,Wei FY,Abe T,Takeda T,Nishiki T,Tomizawa K,Watanabe M,Matsui H,Takei K

doi

10.1111/boc.201500032

subject

Has Abstract

pub_date

2015-09-01 00:00:00

pages

319-30

issue

9

eissn

0248-4900

issn

1768-322X

journal_volume

107

pub_type

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