Abstract:
BACKGROUND INFORMATION:The role of the LIM-domain-containing protein Ajuba was initially described in cell adhesion and migration processes and recently in mitosis as an activator of the Aurora A kinase. RESULTS:In the present study, we show that Ajuba localizes to centrosomes and kinetochores during mitosis. This localization is microtubule-dependent and Ajuba binds microtubules in vitro. A microtubule regrowth assay showed that Ajuba follows nascent microtubules from centrosomes to kinetochores. Owing to its contribution to mitotic commitment and its microtubule-dependent localization, Ajuba could also play a role during the metaphase-anaphase transition. We show that Ajuba interacts with Aurora B and BUBR1 [BUB (budding uninhibited by benomyl)-related 1], two major components of the mitotic checkpoint. Inhibition of BUBR1 by siRNA (small interfering RNA) disrupts chromosome alignment at the metaphase plate and modifies Ajuba localization due to premature mitotic exit. CONCLUSIONS:Ajuba is a microtubule-associated protein that collaborates with Aurora B and BUBR1 at the metaphase-anaphase transition and this may be important to ensure proper chromosome segregation.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Ferrand A,Chevrier V,Chauvin JP,Birnbaum Ddoi
10.1042/BC20080060subject
Has Abstractpub_date
2009-04-01 00:00:00pages
221-35issue
4eissn
0248-4900issn
1768-322Xpii
BC20080060journal_volume
101pub_type
杂志文章abstract:BACKGROUND INFORMATION:The role of the jelly coat that surrounds the amphibian oocytes has been widely discussed, but is poorly understood. The presence of the jelly coat is essential for fertilization. However, the structure and function of the molecules that comprise the jelly coat have not been thoroughly documented...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20050051
更新日期:2006-07-01 00:00:00
abstract::This study was undertaken to evaluate whether hypertrophy and hyperplasia of the pancreatic acinar cells induced by caerulein remained after termination of the hormonal treatment. Rats received subcutaneous injections of saline or caerulein for 4 days and were killed immediately after termination of treatment or 2, 15...
journal_title:Biology of the cell
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doi:
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abstract::The isolation, characterization, and expression of a novel cDNA encoding a Trypanosoma cruzi polypeptide (TcAc2), homologous to various small stress proteins and glutathione S-transferases, are described. The deduced amino-acid sequence revealed two domains sharing 27% identity and an additional 27% similarity to each...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(94)90011-6
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(00)89017-6
更新日期:2000-04-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(00)01101-1
更新日期:2000-10-01 00:00:00
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journal_title:Biology of the cell
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更新日期:1983-01-01 00:00:00
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journal_title:Biology of the cell
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doi:10.1111/boc.202000054
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journal_title:Biology of the cell
pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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journal_title:Biology of the cell
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更新日期:1999-12-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1985.tb00375.x
更新日期:1985-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00
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更新日期:2004-02-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
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journal_title:Biology of the cell
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doi:
更新日期:1999-11-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1989.tb03013.x
更新日期:1989-01-01 00:00:00
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doi:10.1016/j.biolcel.2004.06.002
更新日期:2004-09-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
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journal_title:Biology of the cell
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更新日期:2004-04-01 00:00:00
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journal_title:Biology of the cell
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更新日期:2009-02-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00266.x
更新日期:1984-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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journal_title:Biology of the cell
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更新日期:2003-12-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(02)01220-0
更新日期:2003-01-01 00:00:00
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journal_title:Biology of the cell
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更新日期:2011-10-01 00:00:00