Abstract:
:The nucleolar lesions provoked by the action of ribonuclease (RNase) on living chick embryo fibroblasts were studied by means of microcinematographic analysis and at the ultrastructural level using oxidized diaminobenzidine as a differentially contrasting stain for nucleic acids. This study has shown that the induction of nucleolar dispersion by RNase was only the beginning of a series of discrete steps. The following sequences are described: dispersion of the nucleolus into fragments, their reassembly, and the emission of spherules which appear of chromatin origin. At that step nucleoli are typically segregated. The alteration of the nucleolar associated chromatin seemed to be primordial in these processes. Moreover, the large mass of heterochromatin intimately associated with the nucleolus and which has been considered to be a part of the nucleolar organizer region apparently plays a chief part in the reassembly of the nucleolar fragments into a segregated nucleolus. Ribonuclease is compared to other drugs known to act on nucleolar DNA.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Chobert MN,Pouchelet M,Anteunis A,Gansmuller A,Robineaux Rdoi
10.1111/j.1768-322x.1984.tb00218.xsubject
Has Abstractpub_date
1983-01-01 00:00:00pages
19-28issue
1eissn
0248-4900issn
1768-322Xjournal_volume
49pub_type
杂志文章abstract::HT 29-D4 is a clonal cell line, derived from the human colon adenocarcinoma cell line HT 29, which can be induced to differentiate into enterocyte-like cells by replacing glucose with galactose in the culture medium (Fantini et al. [1986], J. Cell Sci. 83, 235-249). Both undifferentiated and differentiated HT 29-D4 ce...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND INFORMATION:During apoptosis, the first morphological change is a distinct cell shrinkage known as the AVD (apoptotic volume decrease). This event is driven by a loss of intracellular K(+), which creates an osmotic gradient, drawing water out of the cell through AQPs (aquaporins). Loss of water in balance wi...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20040131
更新日期:2006-01-01 00:00:00
abstract::The ciliated protozoan Paramecium harbours a particularly large diversity of microtubule networks, ranging from the elaborate and stable ciliary axonemes and basal bodies to very dynamic cytoplasmic, cortical or intranuclear arrays. Their organization and individual cycle of assembly/disassembly are well known and ext...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(97)89840-1
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND INFORMATION:The ribonucleases (RNases) constitute a heterogeneous group of enzymes, which exert diverse and specific biological functions. Several RNases have been shown to control gene expression and cell differentiation. RNASET2, a novel member of the Rh/T2/S family of RNases, exerts micro-environmental co...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201100092
更新日期:2012-01-01 00:00:00
abstract::The ultrastructure of the Drosophila subobscura chromosome regions around the breakpoints of the complex E1 + 2 + 9 + 12 gene arrangement was analyzed. This overlapping inversion is formed by the association of the E1, E2, E9, and E12 simple inversions. Ultrastructure of sections involving 58D/59A, 61C/D, 62D/63A, 64B...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90359-b
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND INFORMATION:TSPO (translocator protein), previously known as PBR (peripheral-type benzodiazepine receptor), is a ubiquitous 18 kDa transmembrane protein that participates in diverse cell functions. High-affinity TSPO ligands are best known for their ability to stimulate cholesterol transport in organs synthe...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20070157
更新日期:2008-07-01 00:00:00
abstract:BACKGROUND INFORMATION:The identification of a source of stem cells able to regenerate skeletal muscle was the goal of numerous studies with the aim to develop new therapeutic approaches for genetic muscle diseases or muscle injuries. A series of studies have demonstrated that stem cells derived from various tissues ma...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20100112
更新日期:2011-11-01 00:00:00
abstract::Structured mRNA elements called riboswitches control gene expression by binding to small metabolites. Over a dozen riboswitch classes have been characterized that target a broad range of molecules and vary widely in size and secondary structure. Four of the known riboswitch classes recognize purines or modified purine...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20070088
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND INFORMATION:At fertilization in mammalian eggs, the sperm induces a series of Ca(2+) oscillations via the production of inositol 1,4,5-trisphosphate. Increased inositol 1,4,5-trisphosphate production appears to be triggered by a sperm-derived PLCzeta (phospholipase C-zeta) that enters the egg after gamete fu...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20080033
更新日期:2008-11-01 00:00:00
abstract::Revision of the reference map of the polytene chromosomes of Drosophila subobscura was started by means of the electron microscope. We present a map of regions 70B to 72D of the E chromosome obtained from squashed and thin-sectioned salivary glands. It was observed that the total number of bands in divisions 70B to 72...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1988.tb00750.x
更新日期:1988-01-01 00:00:00
abstract::In the present study, we describe the structural and cytological changes observed in staggerer mutant olfactory bulbs, as compared to normal mice. On the basis of photonic and ultrastructural observations we tried to define the alterations induced by the mutation: i.e. a reduction of bulb size, a reduction in the volu...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND INFORMATION:Caspases are cysteine proteases that mediate apoptosis (programmed cell death) initiation and execution. Apoptosis is a conserved mechanism shared by all metazoans, although its physiological function and complexity show considerable taxon-dependent variations. To gain insight into the caspase re...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20050018
更新日期:2005-11-01 00:00:00
abstract::Toad bladder epithelial cells were homogenized and fractionated by Percoll density-gradient centrifugation. Binding of tritium-labeled vasopressin ([3H]AVP) was measured in surface membranes (SM), microsomes (M), and a 100,000 g (60-min) microsomal supernatant fraction (S). More than two-thirds of the total receptors ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND INFORMATION:Proliferating cell nuclear antigen (PCNA) is a key component of the DNA replication machinery involved in the process of DNA elongation, recombination, methylation and repair. We have used PCNA fused with green fluorescent protein (GFP-PCNA) as a convenient tool to show the progress of S-phase in...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20040093
更新日期:2005-03-01 00:00:00
abstract::Fetal calf serum and a serum substitute, Ultroser G, were compared for their effects on the growth curves, clonal growth and cell cycle progression of rabbit chondrocytes in primary culture and during at least three cell passages and included a screen for the maintenance of cartilage-like differentiation i.e. the pres...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00309.x
更新日期:1984-01-01 00:00:00
abstract::The activation of M-phase promoting factor (MPF) in one-cell mouse embryo is independent from the nucleus. Other autonomous phenomena include the cortical activity observed at the end of the first cell cycle and the reorganization of the microtubule network. Here, we observed that the autonomous control of MPF activat...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract:BACKGROUND INFORMATION:Homotypic internalisation of tumour cells has frequently been observed in tumour tissue sections. Events of non-professional phagocytosis, however, may also occur in normal tissue if the number of dying cells exceeds the phagocytic capacity of professional phagocytes such as macrophages and dendr...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201400090
更新日期:2015-10-01 00:00:00
abstract::Actin- and microtubule-based motors can propel different cargos along filaments. Within cells, they control the distribution of membrane-bound compartments by performing complementary tasks. Organelles make long journeys along microtubules, with class V myosins ensuring their capture and their dispersal in actin-rich ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20070021
更新日期:2007-08-01 00:00:00
abstract::Several transformed cells have been demonstrated to secrete growth factors. We studied the effect of conditioned medium from neoplastic rat pleural mesothelial cells on normal and neoplastic mesothelial cell growth. The results showed that the concentrated conditioned medium stimulated neoplastic mesothelial cell grow...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(88)90025-1
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND INFORMATION:Matrix metalloproteinases (MMPs) are the key enzymes responsible for the remodelling of extracellular matrix. Two of them, namely MMP-2 and MMP-9 (gelatinases A and B, respectively), are expressed in skeletal muscles and are involved in their regeneration after the injury. Although MMPs are prima...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201300020
更新日期:2013-08-01 00:00:00
abstract:BACKGROUND INFORMATION:Continued advances in stem cell biology and stem cell transplantation rely on non-invasive biomarkers to characterise cells and stem cell aggregates. The non-invasive quality of such biomarkers is essential because exogenous labels, probes or reporters can unintentionally and dramatically alter s...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201100091
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND INFORMATION:The rod outer segment (OS) is the specialised organelle where phototransduction takes place. Our previous proteomic and biochemical analyses on purified rod disks showed the functional expression of the respiratory chain complexes I-IV and F1 Fo -ATP synthase in OS disks, as well as active solubl...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201300003
更新日期:2013-08-01 00:00:00
abstract:BACKGROUND INFORMATION:PCD (programmed cell death) is a common mechanism to remove unwanted and excessive cells from organisms. In several exocrine cell types, PCD mode of release of secretory products has been reported. The molecular mechanism of the release, however, is largely unknown. Our aim was to study the molec...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20070168
更新日期:2009-02-01 00:00:00
abstract::I am pleased to contribute to this special issue of Biology of the Cell in honor of Yoshio Masui. Oocyte maturation remains a small enough and young enough field that the authors assembled for this issue can trace the entire development of the field over the last 30 years, beginning with the early demonstration by Mas...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:
更新日期:1998-10-01 00:00:00
abstract:BACKGROUND INFORMATION:Meningothelial cells (MECs) are the cellular components of the meninges protecting the brain and as such provide important barrier function for the central nervous system building the interface between neuronal tissue and the cerebrospinal fluid (CSF). MECs were previously shown to be involved in...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201300013
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:The translocase of the mitochondrial inner membrane (TIM) imports most of the nucleus-encoded proteins that are destined for the matrix, inner membrane (IM) and the intermembrane space (IMS). Trypanosoma brucei, the infectious agent for African trypanosomiasis, possesses a unique TIM complex consisting of se...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.202000054
更新日期:2020-10-20 00:00:00
abstract::Human pancreatic ductal cells of the Capan 1 cell line differentiate progressively during growth. After the exponential growth phase, the cells elongate and become polarized with their apical poles covered by microvilli and separated from the basolateral pole by tight junctions. In this stationary phase, they form dom...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(94)80004-9
更新日期:1994-01-01 00:00:00
abstract::In 2001, with-no-lysine (WNK) kinases were identified as the genes responsible for the human hereditary hypertensive disease pseudohypoaldosteronism type II (PHAII). It took a further 6 years to clarify that WNK kinases participate in a signaling cascade with oxidative stress-responsive gene 1 (OSR1), Ste20-related pr...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1111/boc.201300069
更新日期:2014-02-01 00:00:00
abstract::The membrane content in phospholipids along the secretory pathway in rat pancreatic B-cells was studied in situ by high-resolution cytochemistry, applying the recently introduced phospholipase A2-gold technique. The gold particles were mostly associated with cell membranes, and the various types of membranes were labe...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::It is now well established that malignant transformation of eucaryotic cells is concomitant with typical alterations of glycosylation and the expression pattern of endogenous lectins. In parallel, oncogene transfection studies revealed a correlation between the expression of some of these genes, the transformed state ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1111/j.1768-322x.1993.tb00899.x
更新日期:1993-01-01 00:00:00