Culture of chondrocytes in medium supplemented with fetal calf serum or a serum substitute: Ultroser G.

Abstract:

:Fetal calf serum and a serum substitute, Ultroser G, were compared for their effects on the growth curves, clonal growth and cell cycle progression of rabbit chondrocytes in primary culture and during at least three cell passages and included a screen for the maintenance of cartilage-like differentiation i.e. the presence of type II collagen. Proliferation was also compared with another serum substitute, Nu-Serum. Ultroser G is shown to be equivalent to fetal calf serum as far as chondrocyte proliferation is concerned, clonal growth is improved and biosynthesis of type II collagen is maintained in primary culture.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Ronot X,Sene C,Boschetti E,Hartmann DJ,Adolphe M

doi

10.1111/j.1768-322x.1984.tb00309.x

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

307-13

issue

3

eissn

0248-4900

issn

1768-322X

journal_volume

51

pub_type

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