Abstract：
BACKGROUND INFORMATION:TSPO (translocator protein), previously known as PBR (peripheral-type benzodiazepine receptor), is a ubiquitous 18 kDa transmembrane protein that participates in diverse cell functions. High-affinity TSPO ligands are best known for their ability to stimulate cholesterol transport in organs synthesizing steroids and bile salts, although they modulate other physiological functions, including cell proliferation, apoptosis and calcium-dependent transepithelial ion secretion. In present study, we investigated the localization and function of TSPO in salivary glands. RESULTS:Immunohistochemical analysis of TSPO in rat salivary glands revealed that TSPO and its endogenous ligand, DBI (diazepam-binding inhibitor), were present in duct and mucous acinar cells. TSPO was localized to the mitochondria of these cells, whereas DBI was cytosolic. As expected, mitochondrial membrane preparations, which were enriched in TSPO, exhibited a high affinity for the TSPO drug ligand, (3)H-labelled PK 11195, as shown by B(max) and K(d) values of 10.0+/-0.5 pmol/mg and 4.0+/-1.0 nM respectively. Intravenous perfusion of PK 11195 increased the salivary flow rate that was induced by muscarinic and alpha-adrenergic agonists, whereas it had no effect when administered alone. Addition of PK 11195 also increased the K(+), Na(+), Cl(-) and protein content of saliva, indicating that this ligand modulated secretion by acini and duct cells. CONCLUSIONS:High-affinity ligand binding to mitochondrial TSPO modulates neurotransmitter-induced salivary secretion by duct and mucous acinar cells of rat submandibular glands.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Ostuni MA,Tumilasci OR,Péranzi G,Cardoso EM,Contreras LN,Arregger AL,Papadopoulos V,Lacapere JJdoi
10.1042/BC20070157subject
Has Abstractpub_date
2008-07-01 00:00:00pages
427-39issue
7eissn
0248-4900issn
1768-322Xpii
BC20070157journal_volume
100pub_type
杂志文章abstract：BACKGROUND INFORMATION:The transcription factor NFAT (nuclear factor of activated T-cell) family comprises important regulators in immuno-responses and mouse embryonic development, including early cardiovascular and heart valve development. The mechanism involved, however, is not fully understood. Nkx2-5 (NK2 transcrip...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20080108
更新日期：2009-06-01 00:00:00
abstract：:Inside-out porcine thyroid follicles in culture undergo polarity reversal after being embedded in collagen gel. The newly-formed follicles reexpress some specific thyroid functions lost in inside-out follicles (Chambard et al., 1984. We present here an ultrastructural study of the inversion of polarity in this model s...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00463.x
更新日期：1986-01-01 00:00:00
abstract：:The sensory organs of the vertebrate head derive from two embryological structures, the neural crest and the ectodermal placodes. Although quite a lot is known about the secreted and transcription factors that regulate neural crest development, until recently little was known about the molecular pathways that regulate...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20040515
更新日期：2005-05-01 00:00:00
abstract：BACKGROUND:The translocase of the mitochondrial inner membrane (TIM) imports most of the nucleus-encoded proteins that are destined for the matrix, inner membrane (IM) and the intermembrane space (IMS). Trypanosoma brucei, the infectious agent for African trypanosomiasis, possesses a unique TIM complex consisting of se...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.202000054
更新日期：2020-10-20 00:00:00
abstract：:The nucleolar lesions provoked by the action of ribonuclease (RNase) on living chick embryo fibroblasts were studied by means of microcinematographic analysis and at the ultrastructural level using oxidized diaminobenzidine as a differentially contrasting stain for nucleic acids. This study has shown that the inductio...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00218.x
更新日期：1983-01-01 00:00:00
abstract：:The activation of M-phase promoting factor (MPF) in one-cell mouse embryo is independent from the nucleus. Other autonomous phenomena include the cortical activity observed at the end of the first cell cycle and the reorganization of the microtubule network. Here, we observed that the autonomous control of MPF activat...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:Regulated exocytosis of defensive secretory organelles, the trichocysts, as well as a transient Ca(2+)-influx can be induced in Paramecium by aminoethyldextran (Kerboeuf and Cohen, J Cell Biol (1990) 111, 2527). Knoll et al (Febs Lett (1992) 304, 265) reported that veratridine was also a secretagogue for Paramecium. H...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1996.tb00953.x
更新日期：1996-01-01 00:00:00
abstract：:Arabinoxylans were localised by immunocytochemistry using polyclonal antibodies in the cell walls of the apical internode of maize after degradation in the rumen. In order to understand the significance of arabinoxylan in digestibility property, two lines of maize differing in digestibility were used. Wide variations ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1996.tb00988.x
更新日期：1996-01-01 00:00:00
abstract：:Total mRNA extracted from the brain of a marine worm, Nereis diversicolor, were in vitro translated using 2 cell-free systems: rabbit reticulocyte lysate and wheat germ extract. Among numerous products newly translated in both systems, only one class of 70 kDa polypeptides immunoprecipitated when we used a mixture of ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(91)90054-q
更新日期：1991-01-01 00:00:00
abstract：:I am pleased to contribute to this special issue of Biology of the Cell in honor of Yoshio Masui. Oocyte maturation remains a small enough and young enough field that the authors assembled for this issue can trace the entire development of the field over the last 30 years, beginning with the early demonstration by Mas...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1998-10-01 00:00:00
abstract：:In eukaryotes transcription and translation takes place in two different compartments separated by the nuclear envelope. Nuclear translation has been reported in several publications, however, all these published results have been criticized, particularly with respect to cytoplasmic contamination of the nuclei. We hav...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(03)00059-5
更新日期：2003-07-01 00:00:00
abstract：:Vertebrate embryos are able to reconstitute the body plan when early blastomeres are deleted, but it is not known whether this is accomplished by cells local to the lesion or by a readjustment of the entire pattern of the embryo. We distinguished between these two possibilities by studying which embryonic cells change...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/j.biolcel.2004.06.002
更新日期：2004-09-01 00:00:00
abstract：:Genetic studies in the budding yeast have led to the molecular characterization of gamma-tubulin associated proteins and to the identification of orthologues in animal cells. While the gamma-tubulin complex is more complex in animal cells than in budding yeast, its function is probably maintained throughout evolution....
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1999-05-01 00:00:00
abstract：BACKGROUND INFORMATION:PCD (programmed cell death) is a common mechanism to remove unwanted and excessive cells from organisms. In several exocrine cell types, PCD mode of release of secretory products has been reported. The molecular mechanism of the release, however, is largely unknown. Our aim was to study the molec...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20070168
更新日期：2009-02-01 00:00:00
abstract：:We studied formation of domes in cell monolayers of the human colon carcinoma cell line Caco-2 which has been shown to exhibit signs of enterocytic differentiation and transport properties. After a 24 hr incubation with 4 X 10(-8) M ouabain, the number of domes seen on Caco-2 cell monolayers grown on plastic dishes wa...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1985.tb00383.x
更新日期：1985-01-01 00:00:00
abstract：:Netrins are secreted proteins that play a crucial role in neuronal migration and in axon guidance during the development of the nervous system. Netrin-1 has been shown to interact with the transmembrane receptors DCC and UNC5H and these receptors appeared of key importance in mediating the chemotropic activity of netr...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(03)00072-8
更新日期：2003-10-01 00:00:00
abstract：:The entry into, and exit from, mitosis are controlled by a universal M-phase promoting factor (MPF) composed of at least p34cdc2 and a cyclin. Embryonic systems are convenient for studying the association and dissociation of the active MPF complex because oocytes and eggs are naturally arrested at a specific point of ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/0248-4900(92)90137-p
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND INFORMATION:The rod outer segment (OS) is the specialised organelle where phototransduction takes place. Our previous proteomic and biochemical analyses on purified rod disks showed the functional expression of the respiratory chain complexes I-IV and F1 Fo -ATP synthase in OS disks, as well as active solubl...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201300003
更新日期：2013-08-01 00:00:00
abstract：:This review is concerned with the formation during ciliogenesis of centrioles and basal bodies, primarily in epithelial multi-ciliated cells from the developing vertebrate respiratory and reproductive tracts. During ciliated cell differentiation, in these as well as in other cell types, cilium formation is preceded by...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/0248-4900(91)90075-x
更新日期：1991-01-01 00:00:00
abstract：:The internalization of the yolk proteins has been investigated by electron microscopy and cytochemistry in the oocyte of the trout which stores up large quantities of yolk. The oocyte evolution has been followed for 18 months in a homogeneous group of animals. Anionic ferritin has been injected during vitellogenesis. ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00283.x
更新日期：1984-01-01 00:00:00
abstract：:Ciliary receptor cells, typified by cilia or modified cilia, are very common in the animal kingdom. In addition to the cytoskeleton of their ciliary processes these receptors possess other specific prominent cytoskeletal elements. Two representative systems are presented: i) scolopidia, mechanosensitive sensilla of va...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90441-3
更新日期：1992-01-01 00:00:00
abstract：:The mammalian spermatozoa are endowed with a unique cytoskeleton which consists both of ubiquitous and specific proteins, some of them arising from gene haploid transcription. In the head, a dense perinuclear layer is made of basic proteins (calicin, cylicin, etc) associated with calmodulin and actin remnants. In the ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(94)80001-4
更新日期：1994-01-01 00:00:00
abstract：:The pH-related change in morphology of vinblastine (VLB)-induced paracrystals formed in Chinese hamster ovary (CHO) cells was examined immunohistochemically in order to determine both the mechanism of tubulin crystallization and the influence of acidic pHs on cytoskeletal microtubules. Lowering the extracellular pH (p...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(94)90066-3
更新日期：1994-01-01 00:00:00
abstract：:Immunogold labeling and biochemical methods were used to localize the site of viral protein synthesis in frog virus 3 (FV3)-infected baby hamster kidney (BHK) cells. Immunogold labeling studies of Triton-extracted (cytoskeletons), FV3-infected BHK cells with antivimentin antibodies showed that the major components of ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Cilia from quail oviduct were isolated with their membrane. The ultrastructural study revealed a good preservation of cilia in the purified fraction. Electrophoresis on SDS-PAGE showed a reproducible pattern of ciliary proteins, the major bands being those of tubulins 57 kDa and dyneins above 250 kDa. Among the minor ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00487.x
更新日期：1986-01-01 00:00:00
abstract：BACKGROUND INFORMATION:The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90]. RESULTS:In the present study, we cloned an AQP gene from A. proteus [A...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20070091
更新日期：2008-03-01 00:00:00
abstract：:Co-isolated residual nuclear shells and residual nucleoli from membrane-depleted rat liver nuclei were spread according to Kleinschmidt's method. Comparison of the spread residual structures isolated from nuclear shells and spread pore complex-lamina isolated from nuclear envelopes showed that these residual structure...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00438.x
更新日期：1986-01-01 00:00:00
abstract：:The plasma membrane components of the parasitic protozoan Entamoeba histolytica, the causative agent of human invasive amebiasis, have been biochemically and immunologically characterized during the last decade. In addition, genes coding for certain surface proteins have been cloned. In spite of these advances, a unif...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/j.1768-322x.1991.tb03015.x
更新日期：1991-01-01 00:00:00
abstract：:In the present study, we describe the structural and cytological changes observed in staggerer mutant olfactory bulbs, as compared to normal mice. On the basis of photonic and ultrastructural observations we tried to define the alterations induced by the mutation: i.e. a reduction of bulb size, a reduction in the volu...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Since almost two decades, it is known that progesterone is responsible of the release of the prophase I arrest of amphibian oocytes and leads to the activation of the universal MPF, through a puzzling transduction pathway. It involves negative regulation of the cAMP-dependent protein kinase (PKA) and synthesis of new ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(01)01126-1
更新日期：2001-09-01 00:00:00
