Dome formation in the human colon carcinoma cell line Caco-2 in culture. Influence of ouabain and permeable supports.

Abstract:

:We studied formation of domes in cell monolayers of the human colon carcinoma cell line Caco-2 which has been shown to exhibit signs of enterocytic differentiation and transport properties. After a 24 hr incubation with 4 X 10(-8) M ouabain, the number of domes seen on Caco-2 cell monolayers grown on plastic dishes was not significantly altered. After a 90 min preincubation with ouabain, 86rubidium uptake by Caco-2 cells was inhibited by ouabain, indicating that the cells have an ouabain-sensitive Na+, K+-ATPase, while dome formation was unaffected by ouabain. Domes were observed in Caco-2 cell monolayers grown on Nuclepore filters when the pore size was 0.015 micron but not when it was 0.030 micron. Our results suggest that dome formation in the Caco-2 cell line could be independent of Na+, K+-ATPase activity and might be due to accumulation of molecules having an effective hydrodynamic radius comprised between 0.015 and 0.030 micron.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Ramond MJ,Martinot-Peignoux M,Erlinger S

doi

10.1111/j.1768-322x.1985.tb00383.x

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

89-92

issue

1

eissn

0248-4900

issn

1768-322X

journal_volume

54

pub_type

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