Effects of cold shock treatment on amphibian oocytes: alteration of heterogenous nuclear RNP morphology.

Abstract:

:In cold-stressed oocytes of Pleurodeles waltl, lampbrush chromosome lateral loops exhibited important structural modifications which were visualized under light microscopy. Electron microscopy study revealed that the RNP particles associated with growing transcripts in the matrix of these loops were 15 nm at 8 degrees C compared to 30 nm at normal temperature (20 degrees C); hnRNP isolated from cold-stressed oocytes sedimented at 15 S in sucrose, while those from control oocytes sedimented at 30 S, as expected. However, under both normal and cold stress conditions, hnRNP possessed a buoyant density of 1.38 g/cm3 in CsCl, indicating that their typical RNA/protein ratio was maintained at 8 degrees C. Our results demonstrate that cold stress affects the structure of hnRNP in amphibian oocytes.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

N'Da E,Bonnanfant-Jais ML,Moreau N,Angelier N

doi

10.1016/0248-4900(90)90340-9

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

139-51

issue

3

eissn

0248-4900

issn

1768-322X

journal_volume

69

pub_type

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