Abstract：
:For the purpose of investigating the nature of the nervous factor which controls cell proliferation in limb blastema of Newts, we have cultured primary mesenchymous cells from limb blastemas of Axolotl. The cultures were carried out in Petri dishes (Primaria, Falcon) with a basal medium with contained diluted MEM supplemented with hormones (insulin, somatotropin, hydrocortisone and thyroxine). In this medium, the cells disperse from the explant from the 4th day of culture and begin to divide from the 7th day; 3 weeks later the culture begins to decline. During the course of culture, beginning at the 8th day, differentiation of myotubes and chondrogenesis occur. The mitotic index, measured on the 16th day after 48 hr of colchicine treatment, is about 1.6%. Addition of foetal calf serum to the basal medium favours cell migration and survival and stimulates proliferation (mitotic: index 6%); beef embryo extract has no effect on cell migration and a small effect on proliferation (mitotic index: 2.3%). Addition to the basal medium of insulin or nerve extracts (brain and spinal cord of adult newts, brain of 12 days chick embryos) 6 days before we measure the mitotic index stimulates proliferation in proportion to the dose, up to 6 times the mitotic index in basal medium. These results are discussed with respect to the problem of cell proliferation control during limb regeneration.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Albert P,Boilly Bdoi
10.1111/j.1768-322x.1986.tb00512.xsubject
Has Abstractpub_date
1986-01-01 00:00:00pages
251-61issue
3eissn
0248-4900issn
1768-322Xjournal_volume
58pub_type
杂志文章abstract：BACKGROUND INFORMATION:PrAO (primary amine oxidase), also known as SSAO (semicarbazide-sensitive amine oxidase)/VAP-1 (vascular adhesion protein-1), is an enzyme (EC 1.4.3.21) that is highly expressed in blood vessels and participates in many cell processes, including glucose handling or inflammatory leucocyte recruitm...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20110049
更新日期：2011-11-01 00:00:00
abstract：:The development of a single, very large (25-35 microns diameter) nucleolus during oogenesis in the crustacean Acanthocyclops vernalis is described. The nucleolus is the site of ribosomal RNA production in the egg, as shown by in situ hybridization, and apparently the only source, as accessory cells are not observed. R...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：BACKGROUND INFORMATION:The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90]. RESULTS:In the present study, we cloned an AQP gene from A. proteus [A...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20070091
更新日期：2008-03-01 00:00:00
abstract：:Detergent permeabilized Euplotes eurystomus (a fresh water hypotrichous ciliate) was reacted with monoclonal and polyclonal antibodies specific for either detyrosinated or tyrosinated alpha-tubulin (Glu- or Tyr-tubulin). The isolated cytoskeleton-nuclear complex was examined by Western immunoblotting and by immunofluo...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Apoptotic-like events could be rapidly induced by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in cells of the mouse plasmacytoma cell line MPC-11 grown in serum-free medium. Indicators for apoptosis were morphological changes visualized by light and electron microscopy, such as chromatin condensation ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：:Gap junctions are widely expressed in the various cell types of the central nervous system. These specialized membrane intercellular junctions provide the morphological support for direct electrical and biochemical communication between adjacent cells. This intercellular coupling is controlled by neurotransmitters and...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(02)00016-3
更新日期：2002-11-01 00:00:00
abstract：:The low pH dependent membrane fusion reaction responsible for the delivery of the Semliki Forest virus genome into the host cell for replication was visualized by electron microscopy. In order to increase the frequency at which fusion images could be detected a reversible inhibitor, ammonium chloride, was used to sync...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00297.x
更新日期：1984-01-01 00:00:00
abstract：:Oocyte maturation and early development have been intensively studied for well over 100 years. The earliest theory proposed that after fertilisation and during cell division determinants were unequally distributed to control cell fate; experimental proof came from using frog eggs (Roux, 1888). After understanding the ...
journal_title：Biology of the cell
pub_type： 传,社论,历史文章
doi：10.1016/j.biolcel.2004.01.002
更新日期：2004-04-01 00:00:00
abstract：:Aspergillus fumigatus, the causative agent of human aspergillosis, binds to and degrades basement membrane laminin. Using immunoelectron microscopy, laminin binding appeared to be associated with the cell wall expansions of resting conidia, and progressively extended to the outer electron dense layer of the conidial w...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(05)80189-3
更新日期：1993-01-01 00:00:00
abstract：:Genetic studies in the budding yeast have led to the molecular characterization of gamma-tubulin associated proteins and to the identification of orthologues in animal cells. While the gamma-tubulin complex is more complex in animal cells than in budding yeast, its function is probably maintained throughout evolution....
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1999-05-01 00:00:00
abstract：:Lentil seedlings were grown for 28 h in space, on board Spacelab (IML 1 Mission) and growth of the primary root was analysed. The length of cortical cells was less in near weightlessness than on the 1 g centrifuge (flight control) and mitotic index was lower but there was no apparent perturbation in the mitosis. To fu...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(94)90055-8
更新日期：1994-01-01 00:00:00
abstract：:The pH-related change in morphology of vinblastine (VLB)-induced paracrystals formed in Chinese hamster ovary (CHO) cells was examined immunohistochemically in order to determine both the mechanism of tubulin crystallization and the influence of acidic pHs on cytoskeletal microtubules. Lowering the extracellular pH (p...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(94)90066-3
更新日期：1994-01-01 00:00:00
abstract：:The dodecasatellite is a 11/12 bp tandemly repeated sequence which is overrepresented, with regard to its genomic representation, in extrachromosomal circular DNAs from D melanogaster embryos. Here we show that a bacterial plasmid carrying a cluster of dodecasatellite is not able to replicate efficiently in cultured c...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(93)90262-d
更新日期：1993-01-01 00:00:00
abstract：:Detergent extraction of human blood platelets pre-treated with Taxol to stabilize microtubules allows isolation of marginal band (MB) cytoskeletons. We studied MB cytoskeleton structure using dark-field light microscopy and negative stain electron microscopy (EM). Dark-field illumination clearly demonstrated the "hoop...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(88)90002-0
更新日期：1988-01-01 00:00:00
abstract：:Infection with Mycobacterium ulcerans results in a necrotising skin disease known as a Buruli ulcer, the pathology of which is directly linked to the bacterial production of the toxin mycolactone. Recent studies have identified the protein translocation machinery of the endoplasmic reticulum (ER) membrane as the prima...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/boc.201800030
更新日期：2018-11-01 00:00:00
abstract：:The plasma membrane components of the parasitic protozoan Entamoeba histolytica, the causative agent of human invasive amebiasis, have been biochemically and immunologically characterized during the last decade. In addition, genes coding for certain surface proteins have been cloned. In spite of these advances, a unif...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/j.1768-322x.1991.tb03015.x
更新日期：1991-01-01 00:00:00
abstract：:Background information. In vitro-cultured microspores, after an appropriate stress treatment, can switch towards an embryogenic pathway. This process, known as microspore embryogenesis, is an important tool in plant breeding. Basic studies on this process in economically interesting crops, especially in recalcitrant p...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20040142
更新日期：2005-09-01 00:00:00
abstract：:Inside-out porcine thyroid follicles in culture undergo polarity reversal after being embedded in collagen gel. The newly-formed follicles reexpress some specific thyroid functions lost in inside-out follicles (Chambard et al., 1984. We present here an ultrastructural study of the inversion of polarity in this model s...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00463.x
更新日期：1986-01-01 00:00:00
abstract：:We used a high-resolution STEM to attempt to recognize the spatial localization of the macromolecular components of ribosomes isolated from Tetrahymena pyriformis. Images obtained either by the annular dark field detector or by the ratio-contrast mode (Z-contrast) show a ribosome as constituted of rounded dark areas, ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00271.x
更新日期：1984-01-01 00:00:00
abstract：BACKGROUND INFORMATION:In eukaryotic cells, autophagy is considered a lysosomal catabolic process which participates in the degradation of intracellular components in a vacuolar structure termed autolysosome. This pathway plays a significant role in the erythropoiesis process, contributing to the clearance of some orga...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201500058
更新日期：2016-04-01 00:00:00
abstract：:Ultrastructural morphometry (point-counting method) was used to evaluate stereological parameters of rough endoplasmic reticulum (RER), Golgi complex, secretory granules, mitochondria and lysosomes of Rana ridibunda pituitary corticotropic (ACTH) cells in animals subjected to constant environmental conditions (CEC) in...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00472.x
更新日期：1986-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Epithelial cadherin (E-cadherin) is involved in cell-cell adhesion through its extracellular domain, whereas the intracellular domain interacts with adaptor proteins, i.e. β-catenin, links E-cadherin to the actin cytoskeleton and participates in signal transduction events. E-cadherin protects mam...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201300075
更新日期：2014-09-01 00:00:00
abstract：BACKGROUND INFORMATION:Our previous studies have shown that calreticulin, a Ca2+-binding chaperone located in the endoplasmic reticulum, affects cell-substratum adhesions via the induction of vinculin and N-cadherin. Cells overexpressing calreticulin contain more vinculin than low expressers and make abundant contacts ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20060105
更新日期：2007-07-01 00:00:00
abstract：:In the present study, we describe the structural and cytological changes observed in staggerer mutant olfactory bulbs, as compared to normal mice. On the basis of photonic and ultrastructural observations we tried to define the alterations induced by the mutation: i.e. a reduction of bulb size, a reduction in the volu...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:ERM (ezrin/radixin/moesin) proteins, concentrated in actin rich cell-surface structures, cross-link actin filaments with the plasma membrane. They are involved in the formation of microvilli, cell-cell adhesion, maintenance of cell shape, cell motility and membrane trafficking. Recent analyses reveal that they are not...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(00)01078-9
更新日期：2000-08-01 00:00:00
abstract：:A central issue in the understanding of Marfan syndrome deals with the functional architecture of fibrillin-containing microfibrils. Fibrillin-rich microfibrils are long extracellular matrix fibrillar components exhibiting a 50 nm periodic beaded-structure with a width of around 20-25 nm after rotary shadowing and a 1...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(98)80018-x
更新日期：1998-06-01 00:00:00
abstract：:It is now well established that malignant transformation of eucaryotic cells is concomitant with typical alterations of glycosylation and the expression pattern of endogenous lectins. In parallel, oncogene transfection studies revealed a correlation between the expression of some of these genes, the transformed state ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/j.1768-322x.1993.tb00899.x
更新日期：1993-01-01 00:00:00
abstract：:The mammalian spermatozoa are endowed with a unique cytoskeleton which consists both of ubiquitous and specific proteins, some of them arising from gene haploid transcription. In the head, a dense perinuclear layer is made of basic proteins (calicin, cylicin, etc) associated with calmodulin and actin remnants. In the ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(94)80001-4
更新日期：1994-01-01 00:00:00
abstract：BACKGROUND INFORMATION:There are several reports indicating that starved fibroblasts show higher proliferation rates when re-fed with foetal bovine serum. We have evidence demonstrating that this phenomenon is related to secretory proteins which may be beneficial to wound healing. RESULTS:After re-feeding, 16 and 72 h...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201300063
更新日期：2014-05-01 00:00:00
abstract：:Separation by velocity sedimentation at unit gravity according to the STAPUT system of Miller and Phillips was applied to a population of rabbit megakaryocytes previously enriched by density gradient centrifugation. By this means, 80,000 to 100,000 megakaryocytes with 100% purity were collected in eight fractions acco...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00231.x
更新日期：1983-01-01 00:00:00
