Differential distribution of alpha-tubulin isotypes in Euplotes eurystomus determined by confocal immunofluorescence microscopy.

abstract：:Infection with Mycobacterium ulcerans results in a necrotising skin disease known as a Buruli ulcer, the pathology of which is directly linked to the bacterial production of the toxin mycolactone. Recent studies have identified the protein translocation machinery of the endoplasmic reticulum (ER) membrane as the prima...

journal_title：Biology of the cell

authors： Demangel C,High S

更新日期：2018-11-01 00:00:00

abstract：:Aspergillus fumigatus, the causative agent of human aspergillosis, binds to and degrades basement membrane laminin. Using immunoelectron microscopy, laminin binding appeared to be associated with the cell wall expansions of resting conidia, and progressively extended to the outer electron dense layer of the conidial w...

journal_title：Biology of the cell

authors： Tronchin G,Bouchara JP,Larcher G,Lissitzky JC,Chabasse D

更新日期：1993-01-01 00:00:00

abstract：:Image cytometry was applied to study the intracellular localization of autofluorescence and the influence of an oxidative stress on this emission. K562 erythroleukemia cancer cells were analyzed with a microspectrofluorometer, coupled with a Argon laser (Ar+) (363 nm). From each cell, 15 x 15 emission spectra were rec...

journal_title：Biology of the cell

authors： Bondza-Kibangou P,Millot C,Dufer J,Millot JM

更新日期：2001-11-01 00:00:00

abstract：BACKGROUND INFORMATION:During apoptosis, the first morphological change is a distinct cell shrinkage known as the AVD (apoptotic volume decrease). This event is driven by a loss of intracellular K(+), which creates an osmotic gradient, drawing water out of the cell through AQPs (aquaporins). Loss of water in balance wi...

journal_title：Biology of the cell

authors： Jablonski EM,Hughes FM Jr

更新日期：2006-01-01 00:00:00

abstract：:The chromosomal localization of the mouse gene coding for the 68 kDa intermediate filament subunit of neurones (NF-L) was determined by in situ hybridization using specific 3H-labelled DNA probes. There is only one copy of the NF-L gene. The gene encoding NF-L is located on chromosome 14 region (D1-E1). ...

journal_title：Biology of the cell

authors： Mattei MG,Duprey P,Li ZL,Mattei JF,Paulin D

更新日期：1989-01-01 00:00:00

abstract：:Using gold labelled neoglycoproteins containing either alpha-D-glucose, N-acetyl-beta-D-glucosamine, alpha-D-mannose, 6-phospho-alpha-D-mannose, and alpha-L-fucose (BSA), we investigated their intranuclear binding sites in the TG human cell line. Although gold-labelled BSA did not give any noticeable labelling, the pr...

journal_title：Biology of the cell

authors： Bourgeois CA,Monsigny M

更新日期：1990-01-01 00:00:00

abstract：:The study reports morphological growth characteristics and chromosome analysis of neoplastic rat pleural mesothelial cells (RPMC) isolated from a mesothelioma-bearing rat. The pleural mesothelioma was induced by intrapleural injection of chrysotile fibers. Neoplastic RPMC were cultured by the standard methods used for...

journal_title：Biology of the cell

authors： Jaurand MC,Renier A,Van der Meeren A,Magne L,Pinchon MC,Bignon J

更新日期：1986-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Substantial evidence indicates the existence of NCSCs (neural crest-derived stem cells) in embryonic mandibular processes; however, they have not been fully investigated or isolated. The aim of the present study was to isolate stem cells from mandibular process during embryonic development by MAC...

journal_title：Biology of the cell

authors： Zhang J,Duan X,Zhang H,Deng Z,Zhou Z,Wen N,Smith AJ,Zhao W,Jin Y

更新日期：2006-10-01 00:00:00

abstract：:During wound-healing in cultured frog skin fragments, fibronectin (FN) was detected in the dermal-epidermal junction. Intracellular fibronectin was stained using permeabilization and DAB immunoperoxidase. With electron microscopy intracytoplasmic FN granules were localized in the epidermal processes of the stratum ger...

journal_title：Biology of the cell

authors： Denefle JP,Zhu QL,Lechaire JP

更新日期：1989-01-01 00:00:00

abstract：:Background information. Transport of water and small neutral solutes across plasma membranes is facilitated by AQP (aquaporin) and aquaglyceroporin channels, which belong to the MIP (major intrinsic protein) family. So far, more than 800 MIP proteins have been identified on the basis of sequence homology, but only les...

journal_title：Biology of the cell

authors： Hubert JF,Duchesne L,Delamarche C,Vaysse A,Gueuné H,Raguénès-Nicol C

更新日期：2005-09-01 00:00:00

abstract：:An interaction between human poly(A)-binding protein (PABP) et human eRF3 has been demonstrated using a double-hybrid approach and in vitro assays. Here, we show that the binding of both proteins is conserved through evolution. We also demonstrate that the last 39 C-terminal amino acids of PABP contain the interface t...

journal_title：Biology of the cell

authors： Cosson B,Berkova N,Couturier A,Chabelskaya S,Philippe M,Zhouravleva G

更新日期：2002-09-01 00:00:00

abstract：BACKGROUND INFORMATION:Claudin-1 (CLDN1) is a four-span transmembrane protein localised at cell-cell tight junctions (TJs), playing an important role in epithelial impermeability and tissue homoeostasis under physiological conditions. Moreover, CLDN1 expression is up-regulated in several cancers, and the level of CLDN1...

journal_title：Biology of the cell

authors： Clément CMH,Deffieu MS,Dorobantu CM,Baumert TF,Ayala-Nunez NV,Mély Y,Ronde P,Gaudin R

更新日期：2020-05-01 00:00:00

abstract：:RNA helicases of the DEAD-box and related families have been found to be required for all processes involving RNA molecules. Biochemical and genetic analyses have shown that at least two RNA helicases are required for translation initiation in yeast. Although it is generally believed that these enzymes are necessary t...

journal_title：Biology of the cell

authors： Linder P

更新日期：2003-05-01 00:00:00

abstract：:Phosphorylation of the transcription factor CREB appears as an important step in the signal transduction cascade that activates melatonin biosynthesis in the mammalian pineal organ. We have studied the mechanisms causing CREB phosphorylation by immunocytochemical and immunochemical demonstration of phosphorylated CREB...

journal_title：Biology of the cell

authors： Maronde E,Schomerus C,Stehle JH,Korf HW

更新日期：1997-11-01 00:00:00

abstract：:A series of analogs of vasopressin with photoreactive groups in positions 1, 2, 3, 4, 8 or 9 of the nonapeptide sequence have been studied for their effects on water and urea permeability of the isolated toad urinary bladder. Compounds with photoreactive groups in positions 3 or 8 bound covalently to receptors as judg...

journal_title：Biology of the cell

authors： Eggena P,Ma CL,Fahrenholz F,Buku A,Schwartz IL

更新日期：1985-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Caspase-dependent and -independent death mechanisms are involved in apoptosis in a variety of human carcinoma cells treated with antineoplastic compounds. Our laboratory has reported that p53 is a key contributor of mitochondrial apoptosis in cervical carcinoma cells after staurosporine exposure....

journal_title：Biology of the cell

authors： Nicolier M,Decrion-Barthod AZ,Launay S,Prétet JL,Mougin C

更新日期：2009-06-04 00:00:00

abstract：:Hepatitis C virus (HCV) encodes two envelope glycoproteins, E1 and E2, that are released from HCV polyprotein by signal peptidase cleavage. These proteins assemble as a noncovalent heterodimer that is retained in the endoplasmic reticulum. The transmembrane domains of E1 and E2 are multifunctional and play a major rol...

journal_title：Biology of the cell

authors： Voisset C,Dubuisson J

更新日期：2004-08-01 00:00:00

abstract：:We took advantage of one of the main possibilities of ion microscopy, ie isotopic analysis, to study the cellular distribution of molecules labelled either with carbon 14 or with stable isotopes of low natural abundance such as nitrogen 15 and deuterium. The surface of the sample is bombarded with an ion beam (O2+, Cs...

journal_title：Biology of the cell

authors： Hindie E,Coulomb B,Beaupain R,Galle P

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND INFORMATION:NPY (neuropeptide Y) may have an effect on the properties of vascular endothelial cells such as pro-angiogenic effects and potentiation of noradrenaline-induced vasoconstriction. In HUVEC (human umbilical-vein endothelial cells), immunoreactive neuropeptide Y has been detected, but NPY synthesis,...

journal_title：Biology of the cell

authors： Silva AP,Kaufmann JE,Vivancos C,Fakan S,Cavadas C,Shaw P,Brunner HR,Vischer U,Grouzmann E

更新日期：2005-06-01 00:00:00

abstract：:Rotation-mediated aggregate cultures of foetal rat liver cells were prepared and grown in a chemically defined medium. Their capacity for cellular organisation and maturation was studied over a culture period of 3 wk by using both morphologic and biochemical criteria. It was found that within each aggregate, distinct ...

journal_title：Biology of the cell

authors： Guigoz Y,Werffeli P,Favre D,Juillerat M,Wellinger R,Honegger P

更新日期：1987-01-01 00:00:00

abstract：:Bistramide A, a new toxin isolated from a New Caledonian Urochordata, shows an antiproliferative effect on a non-small-cell lung carcinoma line in vitro and G1-blockade. In this work, the growth arrest induced by bistramide A was shown to be irreversible as assessed by growth kinetics of pretreated cells. Furthermore,...

journal_title：Biology of the cell

authors： Riou D,Roussakis C,Robillard N,Biard JF,Verbist JF

更新日期：1993-01-01 00:00:00

abstract：:Much remains to be learned about how cancer cells acquire the property of migration, a prerequisite for invasiveness and metastasis. Loss of p53 functions is assumed to be a crucial step in the development of many types of cancers, leading to dysregulation of cell cycle checkpoint controls and apoptosis. However, emer...

journal_title：Biology of the cell

authors： Roger L,Gadea G,Roux P

更新日期：2006-03-01 00:00:00

abstract：BACKGROUND INFORMATION:The costa is a prominent striated fibre that is found in protozoa of the Trichomonadidae family that present an undulating membrane. It is composed primarily of proteins that have not yet been explored. In this study, we used cell fractionation to obtain a highly enriched costa fraction whose str...

journal_title：Biology of the cell

authors： de Andrade Rosa I,Caruso MB,de Oliveira Santos E,Gonzaga L,Zingali RB,de Vasconcelos ATR,de Souza W,Benchimol M

更新日期：2017-06-01 00:00:00

abstract：:The clonal rat rhabdomyosarcoma cell line BA-HAN-1C is composed of proliferating mononuclear cells, some of which spontaneously fuse to terminally differentiated myotube-like giant cells. This cell line has been shown to be susceptible to differentiation induction with all-trans retinoic acid (RA). Since it is still u...

journal_title：Biology of the cell

authors： Ramp U,Gerharz CD,Eifler E,Biesalski HK,Gabbert HE

更新日期：1994-01-01 00:00:00

abstract：:We have recently shown that staurosporine (ST) can trigger apoptosis of CaSki and HeLa cervical tumor cells from G2/M checkpoint, though the mechanism remains elusive. In this study, we reported that ST induced the inhibition of E6 and E7 viral oncogene and MDM2 expression, while it led to increased levels of p53, whi...

journal_title：Biology of the cell

authors： Bernard B,Prétet JL,Charlot JF,Mougin C

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The interferon (IFN)-inducible protein TRIM22 (Staf50) is a member of the tripartite motif protein family and has been suggested a role in the regulation of viral replication as well as of protein ubiquitylation. In addition, we have previously shown that TRIM22 is a direct target gene for the tu...

journal_title：Biology of the cell

authors： Petersson J,Ageberg M,Sandén C,Olofsson T,Gullberg U,Drott K

更新日期：2012-08-01 00:00:00

abstract：:A central issue in the understanding of Marfan syndrome deals with the functional architecture of fibrillin-containing microfibrils. Fibrillin-rich microfibrils are long extracellular matrix fibrillar components exhibiting a 50 nm periodic beaded-structure with a width of around 20-25 nm after rotary shadowing and a 1...

journal_title：Biology of the cell

authors： Hanssen E,Franc S,Garrone R

更新日期：1998-06-01 00:00:00

abstract：BACKGROUND INFORMATION:Cortactin contributes to growth cone morphogenesis by forming with dynamin, ring-shaped complexes that mechanically bundle and stabilise F-actin. However, the regulatory mechanism of cortactin action is poorly understood. RESULTS:Immunofluorescence microscopy revealed that protein kinase C (PKC)...

journal_title：Biology of the cell

authors： Yamada H,Kikuchi T,Masumoto T,Wei FY,Abe T,Takeda T,Nishiki T,Tomizawa K,Watanabe M,Matsui H,Takei K

更新日期：2015-09-01 00:00:00

abstract：BACKGROUND INFORMATION:Proliferating cell nuclear antigen (PCNA) is a key component of the DNA replication machinery involved in the process of DNA elongation, recombination, methylation and repair. We have used PCNA fused with green fluorescent protein (GFP-PCNA) as a convenient tool to show the progress of S-phase in...

journal_title：Biology of the cell

authors： Kisielewska J,Lu P,Whitaker M

更新日期：2005-03-01 00:00:00

abstract：:The pH-related change in morphology of vinblastine (VLB)-induced paracrystals formed in Chinese hamster ovary (CHO) cells was examined immunohistochemically in order to determine both the mechanism of tubulin crystallization and the influence of acidic pHs on cytoskeletal microtubules. Lowering the extracellular pH (p...

journal_title：Biology of the cell

authors： Takanari H,Morita J,Yamanaka H,Yada K,Takahashi A,Izutsu K

更新日期：1994-01-01 00:00:00