Semliki Forest virus penetration from endosomes: a morphological study.

Abstract:

:The low pH dependent membrane fusion reaction responsible for the delivery of the Semliki Forest virus genome into the host cell for replication was visualized by electron microscopy. In order to increase the frequency at which fusion images could be detected a reversible inhibitor, ammonium chloride, was used to synchronize endosomal acidification, and 20 degrees C incubation was employed to concentrate virus particles into the endosomal compartment.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Helenius A

doi

10.1111/j.1768-322x.1984.tb00297.x

subject

Has Abstract

pub_date

1984-01-01 00:00:00

pages

181-5

issue

2

eissn

0248-4900

issn

1768-322X

journal_volume

51

pub_type

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