Phosphoinositidase C isozymes in SaOS-2 cells: immunocytochemical detection in nuclear and cytoplasmic compartments.

Abstract:

:SaOS-2 cell line presents osteoblastic characteristics which can be modulated by specific agonists involving also phosphoinositide breakdown. In order to determine whether SaOS-2 cells display a phosphoinositide signalling system not only at the cytosol-cell membrane level but also, as recently reported for other cell lines, at the nuclear level, a study has been performed to evaluate the phosphoinositidase C (PIC) activity and to localize different isoforms of PIC in nuclear and cytoplasmic compartments. By immunochemicals methods, and by confocal and electron microscope immunocytochemistry, both PIC beta 1 and gamma 1 have been detected in the nucleus, while only PIC gamma 1 was found in the cytoplasm. A specific association with the inner nuclear matrix has been demonstrated for PIC beta 1 and gamma 1; this latter resulted, on the other hand, in relationship with cytoskeletal filaments after high salt extraction. These findings suggest that these enzymes are not completely soluble but functionally related with cytoskeletal and nucleoskeletal structures.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Maraldi NM,Zini N,Santi S,Bavelloni A,Valmori A,Marmiroli S,Ognibene A

doi

10.1016/0248-4900(93)90143-3

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

243-50

issue

3

eissn

0248-4900

issn

1768-322X

journal_volume

79

pub_type

杂志文章
  • Autonomous activation of histone H1 kinase, cortical activity and microtubule organization in one- and two-cell mouse embryos.

    abstract::The activation of M-phase promoting factor (MPF) in one-cell mouse embryo is independent from the nucleus. Other autonomous phenomena include the cortical activity observed at the end of the first cell cycle and the reorganization of the microtubule network. Here, we observed that the autonomous control of MPF activat...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Ciemerych MA,Tarkowski AK,Kubiak JZ

    更新日期:1998-11-01 00:00:00

  • DNA content and cell cycle analysis by flow cytometry in clinical samples: application in breast cancer.

    abstract::Flow cytometry (FCM) analysis of cellular DNA content has become an increasingly important clinical research tool for the measurement and identification of abnormal cell populations. Examination of cellular DNA content can provide information for use in cell cycle analysis in cancer studies. Most researchers would agr...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/0248-4900(93)90116-v

    authors: Spyratos F

    更新日期:1993-01-01 00:00:00

  • Xenopus death-domain-containing proteins FADD and RIP1 synergistically activate JNK and NF-kappaB.

    abstract:BACKGROUND INFORMATION:Death receptors (DRs) induce intracellular signalling upon engagement of their cognate ligands, leading to apoptosis, cell survival or pro-inflammatory responses. In mammals, DR signalling is mediated by the recruitment of several DD (death domain)-containing molecules, such as FADD (Fas-associat...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20050091

    authors: Ishizawa YH,Tamura K,Yamaguchi T,Matsumoto K,Komiyama M,Takamatsu N,Shiba T,Ito M

    更新日期:2006-08-01 00:00:00

  • Synthesis of melatonin by the pineal modified photoreceptors of birds immunocytochemical localization of hydroxyindole-O-methyltransferase.

    abstract::Photoperiodic control of several biological rhythms is exerted through the inhibitory effect of light on melatonin synthesis in the pineal organ. Hydroxyindole-O-methyltransferase (HIO-MT), the last acting-enzyme in melatonin biosynthesis, constitutes a specific marker of melatoninergic cells. In the present study, an...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(88)90098-6

    authors: Guerlotte J,Voisin P,Brisson P,Faure JP,Collin JP

    更新日期:1988-01-01 00:00:00

  • Immunodetection of lipopolysaccharide in macrophages during the processing of non invasive Shigella dysenteriae.

    abstract::The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(90)90343-2

    authors: Dacosta B,Ryter A,Mounier J,Sansonetti P

    更新日期:1990-01-01 00:00:00

  • The mitogen-inducible gene-6 is involved in regulation of cellular senescence in normal diploid fibroblasts.

    abstract:BACKGROUND INFORMATION:The mitogen-inducible gene-6 (Mig-6) is a non-kinase scaffolding adaptor protein. It has been shown that Mig-6 may play important roles in regulating stress response, maintaining homeostasis and functioning as a tumour suppressor. In this study, we investigated the role of Mig-6 in cellular senes...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201200052

    authors: Xie B,Zhao L,Chen H,Jin B,Mao Z,Yao Z

    更新日期:2013-10-01 00:00:00

  • F3/contactin, a neuronal cell adhesion molecule implicated in axogenesis and myelination.

    abstract::A general feature of the cell adhesion molecules belonging to the immunoglobulin family (Ig-CAMs) is to display a modular structure that provides a framework for multiple binding sites for other recognition molecules. Among this family, F3/contactin is a glycan phosphatidyl-inositol (GPI)-anchored molecule expressed b...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(02)00006-0

    authors: Falk J,Bonnon C,Girault JA,Faivre-Sarrailh C

    更新日期:2002-10-01 00:00:00

  • Golgi complex and lysosomes in rabbit derived Pneumocystis carinii.

    abstract::The ultrastructural morphology of Pneumocystis carinii obtained from nonimmunosuppressed rabbit is described in details. Golgi complex and primary lysosomes of P carinii are described here for the first time. They are easily revealed by the zinc iodide-osmium tetroxide cytochemical reagent. Thiamine pyrophosphatase an...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(90)90362-7

    authors: Palluault F,Dei-Cas E,Slomianny C,Soulez B,Camus D

    更新日期:1990-01-01 00:00:00

  • NFAT directly regulates Nkx2-5 transcription during cardiac cell differentiation.

    abstract:BACKGROUND INFORMATION:The transcription factor NFAT (nuclear factor of activated T-cell) family comprises important regulators in immuno-responses and mouse embryonic development, including early cardiovascular and heart valve development. The mechanism involved, however, is not fully understood. Nkx2-5 (NK2 transcrip...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20080108

    authors: Chen Y,Cao X

    更新日期:2009-06-01 00:00:00

  • Flow DNA analysis in human lung cancer: potentiality and limitations due to sampling methods.

    abstract::Flow DNA analysis was performed on samples from 71 surgically removed lung cancers and from 145 patients undergoing bronchoscopy. Abnormal DNA stem lines, characterized by their DNA index (DI), were frequently observed in operated lung carcinomas (87%). Two or three abnormal DNA stem lines were discovered simultaneous...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1986.tb00494.x

    authors: Chassevent A,Berruchon J,Bertrand G,Chretien MF,Tuchais C,George P,Larra F,Rebel A,Oury M

    更新日期:1986-01-01 00:00:00

  • TPA induces apoptosis in MPC-11 mouse plasmacytoma cells grown in serum-free medium.

    abstract::Apoptotic-like events could be rapidly induced by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in cells of the mouse plasmacytoma cell line MPC-11 grown in serum-free medium. Indicators for apoptosis were morphological changes visualized by light and electron microscopy, such as chromatin condensation ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Giese G,Schmidt J,Gilbert M,Albrecht R,Traub P

    更新日期:1997-05-01 00:00:00

  • Vasopressin-induced transfer via light vesicles of receptors and water channels from basolateral to apical membrane of toad bladder.

    abstract::Toad bladder epithelial cells were homogenized and fractionated by Percoll density-gradient centrifugation. Binding of tritium-labeled vasopressin ([3H]AVP) was measured in surface membranes (SM), microsomes (M), and a 100,000 g (60-min) microsomal supernatant fraction (S). More than two-thirds of the total receptors ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Eggena P,Ma CL

    更新日期:1989-01-01 00:00:00

  • Neuropeptide Y expression, localization and cellular transducing effects in HUVEC.

    abstract:BACKGROUND INFORMATION:NPY (neuropeptide Y) may have an effect on the properties of vascular endothelial cells such as pro-angiogenic effects and potentiation of noradrenaline-induced vasoconstriction. In HUVEC (human umbilical-vein endothelial cells), immunoreactive neuropeptide Y has been detected, but NPY synthesis,...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20040102

    authors: Silva AP,Kaufmann JE,Vivancos C,Fakan S,Cavadas C,Shaw P,Brunner HR,Vischer U,Grouzmann E

    更新日期:2005-06-01 00:00:00

  • Histochemical and biochemical studies of cholinesterases in the carotid labyrinth of amphibians.

    abstract::Acetylcholinesterase activity was detected in the carotid labyrinth of amphibians by both biochemical and histochemical methods. The histochemical tests showed enzyme activity in surfaces of afferent and efferent nerve terminals in contact with type I cells, but none within the type I cells. Acetylcholinesterase activ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00374.x

    authors: Poullet M,Verdiere M

    更新日期:1985-01-01 00:00:00

  • The imaging and quantification of aluminium in the human brain using dynamic secondary ion mass spectrometry (SIMS).

    abstract::Dynamic secondary ion mass spectrometry (SIMS) has been utilised to study the post-mortem distribution of aluminium in air-dried frozen sections from unfixed, unstained human brain in order to minimise contamination of the tissue and avoid redistribution and extraction of endogenous tissue aluminium. Substrates, sputt...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(92)90016-t

    authors: Candy JM,Oakley AE,Mountfort SA,Taylor GA,Morris CM,Bishop HE,Edwardson JA

    更新日期:1992-01-01 00:00:00

  • Effect of translocator protein (18 kDa)-ligand binding on neurotransmitter-induced salivary secretion in rat submandibular glands.

    abstract:BACKGROUND INFORMATION:TSPO (translocator protein), previously known as PBR (peripheral-type benzodiazepine receptor), is a ubiquitous 18 kDa transmembrane protein that participates in diverse cell functions. High-affinity TSPO ligands are best known for their ability to stimulate cholesterol transport in organs synthe...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20070157

    authors: Ostuni MA,Tumilasci OR,Péranzi G,Cardoso EM,Contreras LN,Arregger AL,Papadopoulos V,Lacapere JJ

    更新日期:2008-07-01 00:00:00

  • Image analysis and three-dimensional model of chymotrypsin-transformed human alpha 2-macroglobulin complexed with a monoclonal antibody specific for this conformation.

    abstract::Alpha 2-macroglobulin (alpha 2M) is a plasma inhibitor of proteinases, the steric mechanism of which is based on a considerable conformational change. The typical and distinct H-like shape of alpha 2M-chymotrypsin (alpha 2M-chy) complexes seen by electron microscopy led us to an ultrastructural study of the binding of...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(88)90092-5

    authors: Boisset N,Taveau JC,Barray M,Van Leuven F,Delain E,Lamy JN

    更新日期:1988-01-01 00:00:00

  • Tensins are versatile regulators of Rho GTPase signalling and cell adhesion.

    abstract::Tensins are focal adhesion molecules that were identified and characterised in the late 1980s to early 1990s. They play an essential role in the control of cell adhesion. Tensins can bind the tail of ß integrin via their phospho tyrosine binding domain, they exhibit various protein interaction domains including a Src ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1111/boc.201600053

    authors: Blangy A

    更新日期:2017-03-01 00:00:00

  • RNA interference of metastasis-associated gene 1 inhibits metastasis of B16F10 melanoma cells in a C57BL/6 mouse model.

    abstract:BACKGROUND INFORMATION:MTA1 (metastasis-associated gene 1) has been reported to be overexpressed in cancers with high potential to metastasize. Studies of the molecular mechanisms revealed that MTA1 plays an important role in the process of metastasis of many types of cancer. However, the role of MTA1 in melanoma devel...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/bc20060130

    authors: Qian H,Yu J,Li Y,Wang H,Song C,Zhang X,Liang X,Fu M,Lin C

    更新日期:2007-10-01 00:00:00

  • Cell death, non-invasively assessed by intrinsic fluorescence intensity of NADH, is a predictive indicator of functional differentiation of embryonic stem cells.

    abstract:BACKGROUND INFORMATION:Continued advances in stem cell biology and stem cell transplantation rely on non-invasive biomarkers to characterise cells and stem cell aggregates. The non-invasive quality of such biomarkers is essential because exogenous labels, probes or reporters can unintentionally and dramatically alter s...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201100091

    authors: Buschke DG,Squirrell JM,Fong JJ,Eliceiri KW,Ogle BM

    更新日期:2012-06-01 00:00:00

  • Intracellular trafficking of RNASET2, a novel component of P-bodies.

    abstract:BACKGROUND INFORMATION:The ribonucleases (RNases) constitute a heterogeneous group of enzymes, which exert diverse and specific biological functions. Several RNases have been shown to control gene expression and cell differentiation. RNASET2, a novel member of the Rh/T2/S family of RNases, exerts micro-environmental co...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201100092

    authors: Vidalino L,Monti L,Haase A,Moro A,Acquati F,Taramelli R,Macchi P

    更新日期:2012-01-01 00:00:00

  • Integrin diversity brings specificity in mechanotransduction.

    abstract::Cells sense and respond to the biochemical and physical properties of the extracellular matrix (ECM) through adhesive structures that bridge the cell cytoskeleton and the surrounding environment. Integrin-mediated adhesions interact with specific ECM proteins and sense the rigidity of the substrate to trigger signalli...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1111/boc.201700060

    authors: Seetharaman S,Etienne-Manneville S

    更新日期:2018-03-01 00:00:00

  • Poly(A)-binding protein and eRF3 are associated in vivo in human and Xenopus cells.

    abstract::An interaction between human poly(A)-binding protein (PABP) et human eRF3 has been demonstrated using a double-hybrid approach and in vitro assays. Here, we show that the binding of both proteins is conserved through evolution. We also demonstrate that the last 39 C-terminal amino acids of PABP contain the interface t...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/s0248-4900(02)01194-2

    authors: Cosson B,Berkova N,Couturier A,Chabelskaya S,Philippe M,Zhouravleva G

    更新日期:2002-09-01 00:00:00

  • The occurrence of metals Al, Fe, Ni, Cu, Zn in the nuclei of animal cells: an ultrastructural, in situ, X-ray microanalytical study.

    abstract::Cell nuclei may contain significant quantities of the metals Fe, Ni, Cu, Zn, since they are present in the nucleo-enzymes and/or nucleic acids. These metals have been detected by X-ray microanalysis in situ in dinoflagellates (Kearns et al). Aluminum was only detected in cell nuclei in cases of natural or provoked int...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1987.tb00577.x

    authors: Quintana C,Olmedilla A,Antoine N,Ollacarizqueta A

    更新日期:1987-01-01 00:00:00

  • Triiodothyronine influences quail myoblast proliferation and differentiation.

    abstract::The influence of triiodothyronine (T3) on avian myoblast proliferation and differentiation was studied in secondary cultures using plating densities of 2,500 and 7,000 cells/cm2. Culture media were depleted of T3 (control myoblasts) and increasing amounts were then added to concentrations of 0.6, 3 and 15 nM T3 (treat...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(93)90129-3

    authors: Marchal S,Cassar-Malek I,Pons F,Wrutniak C,Cabello G

    更新日期:1993-01-01 00:00:00

  • Inflammatory environment induces gingival tissue-specific mesenchymal stem cells to differentiate towards a pro-fibrotic phenotype.

    abstract:BACKGROUND INFORMATION:Human gingival tissues are prone to hyperplasia under inflammatory stimuli. We have identified gingival tissue-specific mesenchymal stem cells (GMSCs) and found their functional change being correlated with drug-induced gingival hyperplasia. However, whether these cells exhibit characteristics of...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201200064

    authors: Li N,Liu N,Zhou J,Tang L,Ding B,Duan Y,Jin Y

    更新日期:2013-06-01 00:00:00

  • Bacterial lipopolysaccharide modulates protein kinase C signalling in Lymnaea stagnalis haemocytes.

    abstract::Our knowledge of cell signalling pathways in the molluscan immune system and their response to immunological challenge is currently poor. The present study focused on the Protein Kinase C (PKC) pathway in the immune cells (haemocytes) of Lymnaea stagnalis and its response following exposure to bacterial lipopolysaccha...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/j.biolcel.2003.09.001

    authors: Walker AJ,Plows LD

    更新日期:2003-11-01 00:00:00

  • Construction and characterization of a DNA library from mouse chromosomes 19 purified by flow cytometry.

    abstract::In spite of the constant development concerning physical mapping of eukaryotic genomes, the mouse chromosome 19 remains poorly characterized. In order to improve the possibilities for studying this chromosome, we have constructed a chromosome-specific EcoRI DNA fragment library from mouse chromosomes 19 sorted by flow...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(90)90322-t

    authors: Baron B,Métézeau P,Kiefer-Gachelin H,Goldberg ME

    更新日期:1990-01-01 00:00:00

  • Immunological detection of actin in isolated cilia from quail oviduct.

    abstract::Cilia from quail oviduct were isolated with their membrane. The ultrastructural study revealed a good preservation of cilia in the purified fraction. Electrophoresis on SDS-PAGE showed a reproducible pattern of ciliary proteins, the major bands being those of tubulins 57 kDa and dyneins above 250 kDa. Among the minor ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1986.tb00487.x

    authors: Chailley B,Bork K,Gounon P,Sandoz D

    更新日期:1986-01-01 00:00:00

  • Colocalization of single ribosomes with intermediate filaments in puromycin-treated and serum-starved mouse embryo fibroblasts.

    abstract::Previous experiments have revealed a relatively weak electrostatic binding capacity of in vitro reconstituted intermediate filaments (IFs) as well as of natural IFs of whole cell mount preparations for purified ribosomal particles of mammalian origin. In order to demonstrate that such associations also occur in vivo, ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Traub P,Bauer C,Hartig R,Grüb S,Stahl J

    更新日期:1998-07-01 00:00:00