Synthesis of melatonin by the pineal modified photoreceptors of birds immunocytochemical localization of hydroxyindole-O-methyltransferase.

Abstract:

:Photoperiodic control of several biological rhythms is exerted through the inhibitory effect of light on melatonin synthesis in the pineal organ. Hydroxyindole-O-methyltransferase (HIO-MT), the last acting-enzyme in melatonin biosynthesis, constitutes a specific marker of melatoninergic cells. In the present study, an antibody directed against chicken HIOMT was affinity-purified and used to identify melatoninergic cells in the pineal organ of chicken, quail, sparrow and blackbird. Regardless of the species, intense immunocytochemical reactions were observed in modified photoreceptors, whereas other cellular constituents (mostly glial cells) remained unlabeled. We conclude that modified photoreceptors synthesize melatonin in the avian pineal gland and are thus accountable for the translation of the photoperiodic input into hormonal output.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Guerlotte J,Voisin P,Brisson P,Faure JP,Collin JP

doi

10.1016/0248-4900(88)90098-6

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

93-6

issue

1

eissn

0248-4900

issn

1768-322X

pii

0248-4900(88)90098-6

journal_volume

64

pub_type

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