DNA content and cell cycle analysis by flow cytometry in clinical samples: application in breast cancer.

abstract：BACKGROUND INFORMATION:Available data concerning the sperm morphology of teleost fishes demonstrate wide variation. In the present study, the spermatozoa of Siberian sturgeon (Acipenser baerii Brandt, 1869), a chondrostean fish, was investigated. In contrast with teleost fish, chondrostean spermatozoa have a head with ...

journal_title：Biology of the cell

authors： Psenicka M,Alavi SM,Rodina M,Gela D,Nebesarova J,Linhart O

更新日期：2007-02-01 00:00:00

abstract：BACKGROUND INFORMATION:Claudin-1 (CLDN1) is a four-span transmembrane protein localised at cell-cell tight junctions (TJs), playing an important role in epithelial impermeability and tissue homoeostasis under physiological conditions. Moreover, CLDN1 expression is up-regulated in several cancers, and the level of CLDN1...

journal_title：Biology of the cell

authors： Clément CMH,Deffieu MS,Dorobantu CM,Baumert TF,Ayala-Nunez NV,Mély Y,Ronde P,Gaudin R

更新日期：2020-05-01 00:00:00

abstract：:A central issue in the understanding of Marfan syndrome deals with the functional architecture of fibrillin-containing microfibrils. Fibrillin-rich microfibrils are long extracellular matrix fibrillar components exhibiting a 50 nm periodic beaded-structure with a width of around 20-25 nm after rotary shadowing and a 1...

journal_title：Biology of the cell

authors： Hanssen E,Franc S,Garrone R

更新日期：1998-06-01 00:00:00

abstract：:Using gold labelled neoglycoproteins containing either alpha-D-glucose, N-acetyl-beta-D-glucosamine, alpha-D-mannose, 6-phospho-alpha-D-mannose, and alpha-L-fucose (BSA), we investigated their intranuclear binding sites in the TG human cell line. Although gold-labelled BSA did not give any noticeable labelling, the pr...

journal_title：Biology of the cell

authors： Bourgeois CA,Monsigny M

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Our previous studies have shown that calreticulin, a Ca2+-binding chaperone located in the endoplasmic reticulum, affects cell-substratum adhesions via the induction of vinculin and N-cadherin. Cells overexpressing calreticulin contain more vinculin than low expressers and make abundant contacts ...

journal_title：Biology of the cell

authors： Papp S,Fadel MP,Opas M

更新日期：2007-07-01 00:00:00

abstract：:Antidiuretic hormone induces, in the apical plasma membrane of amphibian urinary bladder epithelial cells, the exocytotic insertion of intramembranous particle aggregates that probably contain water channels. Purification of the apical membrane is a way to characterize the aggregates. The isolation of such purified me...

journal_title：Biology of the cell

authors： Tacnet F,Chevalier J,Coudrier E,Berthonaud V,Ripoche P

更新日期：1989-01-01 00:00:00

abstract：:Oocyte maturation and early development have been intensively studied for well over 100 years. The earliest theory proposed that after fertilisation and during cell division determinants were unequally distributed to control cell fate; experimental proof came from using frog eggs (Roux, 1888). After understanding the ...

journal_title：Biology of the cell

authors： Prigent C,Hunt T

更新日期：2004-04-01 00:00:00

abstract：:In cold-stressed oocytes of Pleurodeles waltl, lampbrush chromosome lateral loops exhibited important structural modifications which were visualized under light microscopy. Electron microscopy study revealed that the RNP particles associated with growing transcripts in the matrix of these loops were 15 nm at 8 degrees...

journal_title：Biology of the cell

authors： N'Da E,Bonnanfant-Jais ML,Moreau N,Angelier N

更新日期：1990-01-01 00:00:00

abstract：:The ultrastructural morphology of Pneumocystis carinii obtained from nonimmunosuppressed rabbit is described in details. Golgi complex and primary lysosomes of P carinii are described here for the first time. They are easily revealed by the zinc iodide-osmium tetroxide cytochemical reagent. Thiamine pyrophosphatase an...

journal_title：Biology of the cell

authors： Palluault F,Dei-Cas E,Slomianny C,Soulez B,Camus D

更新日期：1990-01-01 00:00:00

abstract：:The dynamics of change in mitochondria-rich (MR) cells density in the skin epithelium of Bufo viridis was studied on skin biopsies taken in vivo, throughout experimental periods lasting up to 3 months. When the bathing solution contained Cl-, MR cells' density (Dmrc) greatly decreased. There was one exception, when th...

journal_title：Biology of the cell

authors： Katz U,Gabby S

更新日期：1995-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The costa is a prominent striated fibre that is found in protozoa of the Trichomonadidae family that present an undulating membrane. It is composed primarily of proteins that have not yet been explored. In this study, we used cell fractionation to obtain a highly enriched costa fraction whose str...

journal_title：Biology of the cell

authors： de Andrade Rosa I,Caruso MB,de Oliveira Santos E,Gonzaga L,Zingali RB,de Vasconcelos ATR,de Souza W,Benchimol M

更新日期：2017-06-01 00:00:00

abstract：BACKGROUND INFORMATION:In a previous study, we demonstrated that human neutrophils can develop membrane tubulovesicular extensions (TVEs) that are 160-250 nm in width and several micrometres long. These extensions, or cytonemes, are capable of establishing long-range contacts with other cells or bacteria. Cytonemes con...

journal_title：Biology of the cell

authors： Galkina SI,Fedorova NV,Serebryakova MV,Arifulin EA,Stadnichuk VI,Gaponova TV,Baratova LA,Sud'ina GF

更新日期：2015-05-01 00:00:00

abstract：:Fetal calf serum and a serum substitute, Ultroser G, were compared for their effects on the growth curves, clonal growth and cell cycle progression of rabbit chondrocytes in primary culture and during at least three cell passages and included a screen for the maintenance of cartilage-like differentiation i.e. the pres...

journal_title：Biology of the cell

authors： Ronot X,Sene C,Boschetti E,Hartmann DJ,Adolphe M

更新日期：1984-01-01 00:00:00

abstract：:Variant surface glycoprotein (VSG) of Trypanosoma brucei brucei AnTat 1.1 was released by means of the procedure described by Baltz et al. ([1976], Ann. Immunol. [Inst. Pasteur] 127C, 761-774). The concanavalin-A chromatography yielded 3 VSG fractions according to the addition, in the elution buffer, of alpha-methyl-D...

journal_title：Biology of the cell

authors： Boutignon F,Hublart M,Gomes V,Mendonca-Previato L,Tetaert D,Huet-Duvillier G,Degand P

更新日期：1988-01-01 00:00:00

abstract：:The influence of triiodothyronine (T3) on avian myoblast proliferation and differentiation was studied in secondary cultures using plating densities of 2,500 and 7,000 cells/cm2. Culture media were depleted of T3 (control myoblasts) and increasing amounts were then added to concentrations of 0.6, 3 and 15 nM T3 (treat...

journal_title：Biology of the cell

authors： Marchal S,Cassar-Malek I,Pons F,Wrutniak C,Cabello G

更新日期：1993-01-01 00:00:00

abstract：:A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human ce...

journal_title：Biology of the cell

authors： Philipova RN,Zhelev NZ,Todorov IT,Hadjiolov AA

更新日期：1987-01-01 00:00:00

abstract：:Lentil seedlings were grown for 28 h in space, on board Spacelab (IML 1 Mission) and growth of the primary root was analysed. The length of cortical cells was less in near weightlessness than on the 1 g centrifuge (flight control) and mitotic index was lower but there was no apparent perturbation in the mitosis. To fu...

journal_title：Biology of the cell

authors： Driss-Ecole D,Schoevaert D,Noin M,Perbal G

更新日期：1994-01-01 00:00:00

abstract：BACKGROUND INFORMATION:PCD (programmed cell death) is a common mechanism to remove unwanted and excessive cells from organisms. In several exocrine cell types, PCD mode of release of secretory products has been reported. The molecular mechanism of the release, however, is largely unknown. Our aim was to study the molec...

journal_title：Biology of the cell

authors： Pirger Z,Rácz B,Kiss T

更新日期：2009-02-01 00:00:00

abstract：:Recent advances in understanding the in vitro translocation of nascent polypeptides across the endoplasmic reticulum (ER) membrane have established a molecular basis for the initial reactions predicted by the signal hypothesis. The first two events involve a transient arrest of nascent chain elongation, followed by a ...

journal_title：Biology of the cell

authors： Hortsch M,Meyer DI

更新日期：1984-01-01 00:00:00

abstract：:Toad bladder epithelial cells were homogenized and fractionated by Percoll density-gradient centrifugation. Binding of tritium-labeled vasopressin ([3H]AVP) was measured in surface membranes (SM), microsomes (M), and a 100,000 g (60-min) microsomal supernatant fraction (S). More than two-thirds of the total receptors ...

journal_title：Biology of the cell

authors： Eggena P,Ma CL

更新日期：1989-01-01 00:00:00

abstract：:The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...

journal_title：Biology of the cell

authors： Dacosta B,Ryter A,Mounier J,Sansonetti P

更新日期：1990-01-01 00:00:00

abstract：:The activities of three mycotoxins isolated from the hyphomycete Metarhizium anisopliae: destruxin A, B, and E (DA, DB and DE) are described and compared in vitro on leukemic cells. Their antitumor effect was investigated by flow cytometry on growth, cell viability and cell cycle perturbation 48 h after destruxin expo...

journal_title：Biology of the cell

authors： Odier F,Vey A,Bureau JP

更新日期：1992-01-01 00:00:00

abstract：:The quick-freeze, freeze-fracture, deep-etching and rotary replication techniques were used to analyze the structural organization of Giardia duodenalis. The surface of the flagella was rugous, in contrast to the cell body surface which was smooth. The ventral region was characterized in more detail, exposing the laye...

journal_title：Biology of the cell

authors： Kattenbach WM,Diniz Junior JA,Benchimol M,de Souza W

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The identification of a source of stem cells able to regenerate skeletal muscle was the goal of numerous studies with the aim to develop new therapeutic approaches for genetic muscle diseases or muscle injuries. A series of studies have demonstrated that stem cells derived from various tissues ma...

journal_title：Biology of the cell

authors： Dimicoli-Salazar S,Bulle F,Yacia A,Massé JM,Fichelson S,Vigon I

更新日期：2011-11-01 00:00:00

abstract：BACKGROUND INFORMATION:There are several reports indicating that starved fibroblasts show higher proliferation rates when re-fed with foetal bovine serum. We have evidence demonstrating that this phenomenon is related to secretory proteins which may be beneficial to wound healing. RESULTS:After re-feeding, 16 and 72 h...

journal_title：Biology of the cell

authors： Golpour M,Fattahi S,Niaki HA,Hadipoor A,Abedian Z,Ahangarian GR,Parsian H,Mosapour A,Khorasani HR,Vaziri HR,Bijani A,Mostafazadeh A

更新日期：2014-05-01 00:00:00

abstract：:Aspergillus fumigatus, the causative agent of human aspergillosis, binds to and degrades basement membrane laminin. Using immunoelectron microscopy, laminin binding appeared to be associated with the cell wall expansions of resting conidia, and progressively extended to the outer electron dense layer of the conidial w...

journal_title：Biology of the cell

authors： Tronchin G,Bouchara JP,Larcher G,Lissitzky JC,Chabasse D

更新日期：1993-01-01 00:00:00

abstract：:The sensory organs of the vertebrate head derive from two embryological structures, the neural crest and the ectodermal placodes. Although quite a lot is known about the secreted and transcription factors that regulate neural crest development, until recently little was known about the molecular pathways that regulate...

journal_title：Biology of the cell

authors： Brugmann SA,Moody SA

更新日期：2005-05-01 00:00:00

abstract：:The internalization of the yolk proteins has been investigated by electron microscopy and cytochemistry in the oocyte of the trout which stores up large quantities of yolk. The oocyte evolution has been followed for 18 months in a homogeneous group of animals. Anionic ferritin has been injected during vitellogenesis. ...

journal_title：Biology of the cell

authors： Busson-Mabillot S

更新日期：1984-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The Wiskott-Aldrich syndrome protein and scar homolog (WASH) complex is the major Arp2/3 activator at the surface of endosomes. The branched actin network, that the WASH complex induces, contributes to cargo sorting and scission of transport intermediates destined for most endosomal routes. A maj...

journal_title：Biology of the cell

authors： Helfer E,Harbour ME,Henriot V,Lakisic G,Sousa-Blin C,Volceanov L,Seaman MNJ,Gautreau A

更新日期：2013-05-01 00:00:00

abstract：:Detergent permeabilized Euplotes eurystomus (a fresh water hypotrichous ciliate) was reacted with monoclonal and polyclonal antibodies specific for either detyrosinated or tyrosinated alpha-tubulin (Glu- or Tyr-tubulin). The isolated cytoskeleton-nuclear complex was examined by Western immunoblotting and by immunofluo...

journal_title：Biology of the cell

authors： Olins DE,Olins AL,Robert-Nicoud M,Jovin TM,Wehland J,Weber K

更新日期：1989-01-01 00:00:00