Abstract：
:A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human cell lines and phytohemagglutinin-stimulated lymphocytes, but absence in resting lymphocytes; (b) appearance in stimulated lymphocytes in parallel with the onset of DNA synthesis; (c) a speckled distribution in the nucleoplasm; (d) tight association with nuclear matrix structures identified by both biochemical and in situ extraction and enzyme treatment procedures; (e) mol wt of 125 kDa and pI 6.5 as determined by immunoprecipitation or immunoblotting of nuclear or nuclear matrix proteins fractionated by gel electrophoresis. The above characteristics identify the p125/6.5 nuclear matrix protein recognized by the isolated monoclonal antibody as belonging to the class of proliferating cell nuclear antigens.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Philipova RN,Zhelev NZ,Todorov IT,Hadjiolov AAdoi
10.1111/j.1768-322x.1987.tb00539.xsubject
Has Abstractpub_date
1987-01-01 00:00:00pages
1-8issue
1eissn
0248-4900issn
1768-322Xjournal_volume
60pub_type
杂志文章abstract：:The lateral line of the zebrafish has many of the advantages that made the sensory organs of Drosophila a very productive model system: 1) it comprises a set of discrete sense organs (neuromasts) arranged in a defined, species-specific pattern, such that each organ can be individually recognized; 2) the neuromasts are...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/j.biolcel.2003.10.005
更新日期：2003-12-01 00:00:00
abstract：:Circulating glucocorticoid (GC) levels are thought to modulate the basal activity of pro-opiomelanocortin (POMC) neurons within the mediobasal hypothalamus (MBH) of the male rat. In a recent study we demonstrated that Fos-immunoreactivity (Fos-IR) was spontaneously induced throughout the dark phase of the light/dark c...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1997-12-01 00:00:00
abstract：BACKGROUND INFORMATION:Available data concerning the sperm morphology of teleost fishes demonstrate wide variation. In the present study, the spermatozoa of Siberian sturgeon (Acipenser baerii Brandt, 1869), a chondrostean fish, was investigated. In contrast with teleost fish, chondrostean spermatozoa have a head with ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20060060
更新日期：2007-02-01 00:00:00
abstract：BACKGROUND INFORMATION:There have been several studies to understand the influence of stiffness of the culture substrates for different types of adherent cells. It is generally accepted that cell proliferation, spreading and focal adhesions increase with higher matrix stiffness. However, what remains unclear is whether...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201900115
更新日期：2020-10-01 00:00:00
abstract：:The activities of three mycotoxins isolated from the hyphomycete Metarhizium anisopliae: destruxin A, B, and E (DA, DB and DE) are described and compared in vitro on leukemic cells. Their antitumor effect was investigated by flow cytometry on growth, cell viability and cell cycle perturbation 48 h after destruxin expo...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90037-2
更新日期：1992-01-01 00:00:00
abstract：:Background information. In vitro-cultured microspores, after an appropriate stress treatment, can switch towards an embryogenic pathway. This process, known as microspore embryogenesis, is an important tool in plant breeding. Basic studies on this process in economically interesting crops, especially in recalcitrant p...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20040142
更新日期：2005-09-01 00:00:00
abstract：:Cells sense and respond to the biochemical and physical properties of the extracellular matrix (ECM) through adhesive structures that bridge the cell cytoskeleton and the surrounding environment. Integrin-mediated adhesions interact with specific ECM proteins and sense the rigidity of the substrate to trigger signalli...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/boc.201700060
更新日期：2018-03-01 00:00:00
abstract：:The PAS-positive-calcium-sensitive (Ca-s) cells of the pars intermedia (PI) were studied in goldfish kept in fresh water (FW), deionized water (DW), 1/3 sea water (SW) and 1/3 Ca-free SW. Ultrastructural studies show that Ca-s cells of control goldfish kept in FW have a low activity with elongated or deeply indented n...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00202.x
更新日期：1983-01-01 00:00:00
abstract：:The influence of triiodothyronine (T3) on avian myoblast proliferation and differentiation was studied in secondary cultures using plating densities of 2,500 and 7,000 cells/cm2. Culture media were depleted of T3 (control myoblasts) and increasing amounts were then added to concentrations of 0.6, 3 and 15 nM T3 (treat...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(93)90129-3
更新日期：1993-01-01 00:00:00
abstract：:Much remains to be learned about how cancer cells acquire the property of migration, a prerequisite for invasiveness and metastasis. Loss of p53 functions is assumed to be a crucial step in the development of many types of cancers, leading to dysregulation of cell cycle checkpoint controls and apoptosis. However, emer...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20050058
更新日期：2006-03-01 00:00:00
abstract：:A quantitative ultrastructural study was performed on 56 ejaculates showing anomalies of the sperm axonemal complex. The anomalies comprised either the absence of one, or more often several, axonemal structures, or defective elongation of the doublets. Several characteristics relating to the extent and superimposition...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00253.x
更新日期：1984-01-01 00:00:00
abstract：:Lentil seedlings were grown for 28 h in space, on board Spacelab (IML 1 Mission) and growth of the primary root was analysed. The length of cortical cells was less in near weightlessness than on the 1 g centrifuge (flight control) and mitotic index was lower but there was no apparent perturbation in the mitosis. To fu...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(94)90055-8
更新日期：1994-01-01 00:00:00
abstract：:Effect of estradiol-17 beta on rat vaginal epithelial cells (VEC) was studied by transmission electron microscopy. During the normal estrous cycle in adult rats keratinization and exfoliation of VEC was observed only in the estrus phase, though tonofilament bundles were seen distributed in the cytoplasm of the VEC dur...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The membrane content in phospholipids along the secretory pathway in rat pancreatic B-cells was studied in situ by high-resolution cytochemistry, applying the recently introduced phospholipase A2-gold technique. The gold particles were mostly associated with cell membranes, and the various types of membranes were labe...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The entry into, and exit from, mitosis are controlled by a universal M-phase promoting factor (MPF) composed of at least p34cdc2 and a cyclin. Embryonic systems are convenient for studying the association and dissociation of the active MPF complex because oocytes and eggs are naturally arrested at a specific point of ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/0248-4900(92)90137-p
更新日期：1992-01-01 00:00:00
abstract：:Flow DNA analysis was performed on samples from 71 surgically removed lung cancers and from 145 patients undergoing bronchoscopy. Abnormal DNA stem lines, characterized by their DNA index (DI), were frequently observed in operated lung carcinomas (87%). Two or three abnormal DNA stem lines were discovered simultaneous...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00494.x
更新日期：1986-01-01 00:00:00
abstract：:Primary cultures of cells from breast carcinomas were attempted in 74 cases. Growth was observed in 46 cases. Using immunochemical demonstration of keratin proteins (KER), epithelial membrane antigen (EMA) and carcinoembryonic antigen (CEA), three morphologically distinct cell populations were characterized and descri...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00574.x
更新日期：1987-01-01 00:00:00
abstract：:The activation of M-phase promoting factor (MPF) in one-cell mouse embryo is independent from the nucleus. Other autonomous phenomena include the cortical activity observed at the end of the first cell cycle and the reorganization of the microtubule network. Here, we observed that the autonomous control of MPF activat...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：BACKGROUND:The translocase of the mitochondrial inner membrane (TIM) imports most of the nucleus-encoded proteins that are destined for the matrix, inner membrane (IM) and the intermembrane space (IMS). Trypanosoma brucei, the infectious agent for African trypanosomiasis, possesses a unique TIM complex consisting of se...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.202000054
更新日期：2020-10-20 00:00:00
abstract：:Porcine lutropin (pLH) and human choriogonadotropin (hCG) recognize the same hormonal receptor and elicit the same steroidogenic response in porcine Leydig cells in primary culture. We compared the variation in the number of occupied and free receptors present on the cell surface under short-term stimulation by the tw...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00523.x
更新日期：1987-01-01 00:00:00
abstract：:The ultrastructural morphology of Pneumocystis carinii obtained from nonimmunosuppressed rabbit is described in details. Golgi complex and primary lysosomes of P carinii are described here for the first time. They are easily revealed by the zinc iodide-osmium tetroxide cytochemical reagent. Thiamine pyrophosphatase an...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90362-7
更新日期：1990-01-01 00:00:00
abstract：:Acetylcholinesterase activity was detected in the carotid labyrinth of amphibians by both biochemical and histochemical methods. The histochemical tests showed enzyme activity in surfaces of afferent and efferent nerve terminals in contact with type I cells, but none within the type I cells. Acetylcholinesterase activ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1985.tb00374.x
更新日期：1985-01-01 00:00:00
abstract：:Extensive developmentally programmed DNA rearrangements, including thousands of internal deletions, occur in the differentiating somatic macronucleus in Tetrahymena thermophila. Some deletion systems involve the use of multiple alternative deletion sites. We report here the cloning and the sequences of three new alter...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(96)84773-3
更新日期：1996-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Autofluorescence spectroscopy is a powerful tool for molecular histology and for following metabolic processes in biological samples as it does not require labelling. However, at the microscopic scale, it is mostly limited to visible and near infrared excitation of the samples. Several interestin...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201200075
更新日期：2013-07-01 00:00:00
abstract：:The plasma membrane components of the parasitic protozoan Entamoeba histolytica, the causative agent of human invasive amebiasis, have been biochemically and immunologically characterized during the last decade. In addition, genes coding for certain surface proteins have been cloned. In spite of these advances, a unif...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/j.1768-322x.1991.tb03015.x
更新日期：1991-01-01 00:00:00
abstract：:The pH-related change in morphology of vinblastine (VLB)-induced paracrystals formed in Chinese hamster ovary (CHO) cells was examined immunohistochemically in order to determine both the mechanism of tubulin crystallization and the influence of acidic pHs on cytoskeletal microtubules. Lowering the extracellular pH (p...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(94)90066-3
更新日期：1994-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Homotypic internalisation of tumour cells has frequently been observed in tumour tissue sections. Events of non-professional phagocytosis, however, may also occur in normal tissue if the number of dying cells exceeds the phagocytic capacity of professional phagocytes such as macrophages and dendr...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201400090
更新日期：2015-10-01 00:00:00
abstract：:Several transformed cells have been demonstrated to secrete growth factors. We studied the effect of conditioned medium from neoplastic rat pleural mesothelial cells on normal and neoplastic mesothelial cell growth. The results showed that the concentrated conditioned medium stimulated neoplastic mesothelial cell grow...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(88)90025-1
更新日期：1988-01-01 00:00:00
abstract：:Bistramide A, a new toxin isolated from a New Caledonian Urochordata, shows an antiproliferative effect on a non-small-cell lung carcinoma line in vitro and G1-blockade. In this work, the growth arrest induced by bistramide A was shown to be irreversible as assessed by growth kinetics of pretreated cells. Furthermore,...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(05)80196-0
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND INFORMATION:MTA1 (metastasis-associated gene 1) has been reported to be overexpressed in cancers with high potential to metastasize. Studies of the molecular mechanisms revealed that MTA1 plays an important role in the process of metastasis of many types of cancer. However, the role of MTA1 in melanoma devel...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/bc20060130
更新日期：2007-10-01 00:00:00
