Abstract：
:Extensive developmentally programmed DNA rearrangements, including thousands of internal deletions, occur in the differentiating somatic macronucleus in Tetrahymena thermophila. Some deletion systems involve the use of multiple alternative deletion sites. We report here the cloning and the sequences of three new alternative deletion systems (RR, RP and B) obtained using genomic subtraction. The RP and RR deletion systems are 2 kb apart on chromosome 1R, and both involve the removal of < 2 kb of micronuclear sequences. The B deletion system is on chromosome 5 and involves a deletion of > 5 kb. All three deleted regions are very AT rich (approximately 80%) and do not appear to encode any protein. Sequences of the regions flanking the deletion junctions of all three systems revealed no sequence similarity among them nor with any previously reported deletion systems, suggesting that different cis-acting elements are involved for rearrangement. Unlike other deletion systems in ciliates, the B deletion system lacks short terminal direct repeats. Our results suggest an average of at least one alternative deletion system per 134 kb of micronuclear DNA and lead to an estimate that at least 25% of all deletion systems in Tetrahymena utilize alternative ends. The genomic subtraction method employed in this study could prove useful for the isolation of alternatively deleted DNA in special-purpose cases in Tetrahymena and other ciliates. The hybridization parameters for genomic subtraction worked out here for highly AT-rich DNA may have wider usefulness.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Chau MF,Orias Edoi
10.1016/0248-4900(96)84773-3subject
Has Abstractpub_date
1996-01-01 00:00:00pages
111-20issue
2-3eissn
0248-4900issn
1768-322Xjournal_volume
86pub_type
杂志文章abstract：:I am pleased to contribute to this special issue of Biology of the Cell in honor of Yoshio Masui. Oocyte maturation remains a small enough and young enough field that the authors assembled for this issue can trace the entire development of the field over the last 30 years, beginning with the early demonstration by Mas...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1998-10-01 00:00:00
abstract：:Ciliary receptor cells, typified by cilia or modified cilia, are very common in the animal kingdom. In addition to the cytoskeleton of their ciliary processes these receptors possess other specific prominent cytoskeletal elements. Two representative systems are presented: i) scolopidia, mechanosensitive sensilla of va...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90441-3
更新日期：1992-01-01 00:00:00
abstract：:During wound-healing in cultured frog skin fragments, fibronectin (FN) was detected in the dermal-epidermal junction. Intracellular fibronectin was stained using permeabilization and DAB immunoperoxidase. With electron microscopy intracytoplasmic FN granules were localized in the epidermal processes of the stratum ger...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1989.tb03013.x
更新日期：1989-01-01 00:00:00
abstract：:Multidrug-resistant (MDR) variants of a human osteosarcoma cell line (U-2 OS) have been recently obtained by continuous exposure to doxorubicin (DX). The growth and phenotypic characteristics of these cell lines have been demonstrated to be related to the level of expression of P-glycoprotein. In this work, the morpho...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(96)89429-9
更新日期：1995-01-01 00:00:00
abstract：:The dermis of the frog skin (Rana esculenta) displayed a remarkable organization of vertical and horizontal tracts. Vertical thick tracts connected the dermal Stratum spongiosum with the subcutaneous tissue. Horizontal thin tracts were found alongside and contiguous to them. The thick tracts were sheathed by collagen ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00533.x
更新日期：1987-01-01 00:00:00
abstract：BACKGROUND INFORMATION:The role of the LIM-domain-containing protein Ajuba was initially described in cell adhesion and migration processes and recently in mitosis as an activator of the Aurora A kinase. RESULTS:In the present study, we show that Ajuba localizes to centrosomes and kinetochores during mitosis. This loc...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20080060
更新日期：2009-04-01 00:00:00
abstract：BACKGROUND INFORMATION:MTA1 (metastasis-associated gene 1) has been reported to be overexpressed in cancers with high potential to metastasize. Studies of the molecular mechanisms revealed that MTA1 plays an important role in the process of metastasis of many types of cancer. However, the role of MTA1 in melanoma devel...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/bc20060130
更新日期：2007-10-01 00:00:00
abstract：:The clonal rat rhabdomyosarcoma cell line BA-HAN-1C is composed of proliferating mononuclear cells, some of which spontaneously fuse to terminally differentiated myotube-like giant cells. This cell line has been shown to be susceptible to differentiation induction with all-trans retinoic acid (RA). Since it is still u...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(94)90052-3
更新日期：1994-01-01 00:00:00
abstract：:Although there are many macromolecular complexes which play extremely important roles in biology, and despite continued progress in X-ray crystallographic and NMR methods, it is still very difficult to obtain atomic level structural information about such large assemblies. It is now clear that a powerful approach is t...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/j.1768-322x.1994.tb00932.x
更新日期：1994-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Our previous studies have shown that calreticulin, a Ca2+-binding chaperone located in the endoplasmic reticulum, affects cell-substratum adhesions via the induction of vinculin and N-cadherin. Cells overexpressing calreticulin contain more vinculin than low expressers and make abundant contacts ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20060105
更新日期：2007-07-01 00:00:00
abstract：:Cells sense and respond to the biochemical and physical properties of the extracellular matrix (ECM) through adhesive structures that bridge the cell cytoskeleton and the surrounding environment. Integrin-mediated adhesions interact with specific ECM proteins and sense the rigidity of the substrate to trigger signalli...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/boc.201700060
更新日期：2018-03-01 00:00:00
abstract：:The ciliated protozoan Paramecium harbours a particularly large diversity of microtubule networks, ranging from the elaborate and stable ciliary axonemes and basal bodies to very dynamic cytoplasmic, cortical or intranuclear arrays. Their organization and individual cycle of assembly/disassembly are well known and ext...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(97)89840-1
更新日期：1996-01-01 00:00:00
abstract：:Infection with Mycobacterium ulcerans results in a necrotising skin disease known as a Buruli ulcer, the pathology of which is directly linked to the bacterial production of the toxin mycolactone. Recent studies have identified the protein translocation machinery of the endoplasmic reticulum (ER) membrane as the prima...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/boc.201800030
更新日期：2018-11-01 00:00:00
abstract：:Rotation-mediated aggregate cultures of foetal rat liver cells were prepared and grown in a chemically defined medium. Their capacity for cellular organisation and maturation was studied over a culture period of 3 wk by using both morphologic and biochemical criteria. It was found that within each aggregate, distinct ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00555.x
更新日期：1987-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Nucleolin is a major nucleolar protein which is highly expressed in rapidly dividing cells and cancer cell lines. This protein is claimed to be multifunctional and could play a role in rRNA (ribosomal RNA) synthesis, as well as in cell division or response to cellular stresses. Therefore, how nuc...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20080054
更新日期：2009-03-01 00:00:00
abstract：:Membrane fluidity of erythrocytes obtained from 15 children with trisomy 21 and 20 healthy controls were studied by measuring steady-state fluorescence anisotropy and fluorescence lifetime of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in hemog...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90133-l
更新日期：1992-01-01 00:00:00
abstract：:One of the initial events required for the expression of cartilage-specific macromolecules in monolayer cultures is the reversion to the initial round shape of chondrocytes. Thus, considerable research efforts have focused on developing reliable procedures to maintain a round morphology of cultured chondrocytes. Our s...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(96)81303-7
更新日期：1995-01-01 00:00:00
abstract：:Structured mRNA elements called riboswitches control gene expression by binding to small metabolites. Over a dozen riboswitch classes have been characterized that target a broad range of molecules and vary widely in size and secondary structure. Four of the known riboswitch classes recognize purines or modified purine...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20070088
更新日期：2008-01-01 00:00:00
abstract：:The ultrastructural morphology of Pneumocystis carinii obtained from nonimmunosuppressed rabbit is described in details. Golgi complex and primary lysosomes of P carinii are described here for the first time. They are easily revealed by the zinc iodide-osmium tetroxide cytochemical reagent. Thiamine pyrophosphatase an...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90362-7
更新日期：1990-01-01 00:00:00
abstract：:Co-isolated residual nuclear shells and residual nucleoli from membrane-depleted rat liver nuclei were spread according to Kleinschmidt's method. Comparison of the spread residual structures isolated from nuclear shells and spread pore complex-lamina isolated from nuclear envelopes showed that these residual structure...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00438.x
更新日期：1986-01-01 00:00:00
abstract：:As a rule, chloride movement (JC1-) across amphibian skin is considered to be passive; this is implied in fact for preparations incubated in Ringer's fluid, since short-circuit current (Isc) is the quantitative expression of net, active sodium transport (JNa+). The nature of the Cl- pathway(s) was investigated by incu...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Acetylcholinesterase activity was detected in the carotid labyrinth of amphibians by both biochemical and histochemical methods. The histochemical tests showed enzyme activity in surfaces of afferent and efferent nerve terminals in contact with type I cells, but none within the type I cells. Acetylcholinesterase activ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1985.tb00374.x
更新日期：1985-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Netrin-1 is a bi-functional cue that attracts or repels different classes of neurons during development. The netrin-1 receptor DCC (deleted in colorectal cancer) acts as a tyrosine kinase-associated receptor to mediate the attractive response towards netrin-1. The lipid raft-localized Src family ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20070108
更新日期：2009-02-01 00:00:00
abstract：:The highly conserved VICKZ (Vg1 RBP/Vera, IMP-1,2,3, CRD-BP, KOC, ZBP-1) family of RNA-binding proteins recognize specific cis-acting elements in a variety of different RNAs and have been implicated in cell polarity and migration, cell proliferation, and cancer. In just the last two years, the use of transgenic mice, ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20040151
更新日期：2005-01-01 00:00:00
abstract：:Antidiuretic hormone induces, in the apical plasma membrane of amphibian urinary bladder epithelial cells, the exocytotic insertion of intramembranous particle aggregates that probably contain water channels. Purification of the apical membrane is a way to characterize the aggregates. The isolation of such purified me...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Ultrastructural morphometry (point-counting method) was used to evaluate stereological parameters of rough endoplasmic reticulum (RER), Golgi complex, secretory granules, mitochondria and lysosomes of Rana ridibunda pituitary corticotropic (ACTH) cells in animals subjected to constant environmental conditions (CEC) in...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00472.x
更新日期：1986-01-01 00:00:00
abstract：:We used a high-resolution STEM to attempt to recognize the spatial localization of the macromolecular components of ribosomes isolated from Tetrahymena pyriformis. Images obtained either by the annular dark field detector or by the ratio-contrast mode (Z-contrast) show a ribosome as constituted of rounded dark areas, ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00271.x
更新日期：1984-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Matrix metalloproteinases (MMPs) are the key enzymes responsible for the remodelling of extracellular matrix. Two of them, namely MMP-2 and MMP-9 (gelatinases A and B, respectively), are expressed in skeletal muscles and are involved in their regeneration after the injury. Although MMPs are prima...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201300020
更新日期：2013-08-01 00:00:00
abstract：BACKGROUND INFORMATION:PCD (programmed cell death) is a common mechanism to remove unwanted and excessive cells from organisms. In several exocrine cell types, PCD mode of release of secretory products has been reported. The molecular mechanism of the release, however, is largely unknown. Our aim was to study the molec...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20070168
更新日期：2009-02-01 00:00:00
abstract：:The occurrence of the tubulin post-translational modification, polyglycylation, in stable microtubular structures was investigated during morphogenesis in two ciliates, Paramecium and Frontonia atra, belonging to the Epiplasmata group. This analysis was carried out by means of immunofluorescence and post-embedding imm...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(00)01107-2
更新日期：2000-12-01 00:00:00
