Golgi complex and lysosomes in rabbit derived Pneumocystis carinii.

Abstract:

:The ultrastructural morphology of Pneumocystis carinii obtained from nonimmunosuppressed rabbit is described in details. Golgi complex and primary lysosomes of P carinii are described here for the first time. They are easily revealed by the zinc iodide-osmium tetroxide cytochemical reagent. Thiamine pyrophosphatase and beta-glycerophosphatase activities are found in the parasite but cytidine 5' monophosphatase activity is not observed. A weak thiamine pyrophosphatase activity is detected in Golgi vesicles. An endomembranous saccular structure, present from the intracystic body stage to the precystic stage, apparently plays the role of secondary lysosome. A second type of endomembranous saccular structure, only present in the well developed trophozoitic and precystic forms is also described. The presence of carbohydrates in the cell wall of the parasite was demonstrated by periodic acid-thiosemicarbazide-silver proteinate staining and lectin concanavalin A labeling. The development of Golgi vesicles preceded the transition from double-layered to three-layered parasite stages.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Palluault F,Dei-Cas E,Slomianny C,Soulez B,Camus D

doi

10.1016/0248-4900(90)90362-7

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

73-82

issue

1-2

eissn

0248-4900

issn

1768-322X

journal_volume

70

pub_type

杂志文章
  • Developmental and cellular functions of the ESCRT machinery in pluricellular organisms.

    abstract::ESCRTs (endosomal sorting complexes required for transport) were first discovered in yeast and are known to be required in the biogenesis of the MVB (multivesicular body). Most ESCRT research has been carried out in vitro using models such as yeast and mammalian cells in culture. The role of the ESCRTs genes in endoso...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1042/BC20090145

    authors: Michelet X,Djeddi A,Legouis R

    更新日期:2010-01-13 00:00:00

  • Localization by immunoelectron microscopy of somatostatin-containing cells in the gastric mucosa of a teleost fish, Perca fluviatilis.

    abstract::Somatostatin-immunoreactive cells were localized on semithin and ultrathin sections of Epon-embedded samples of perch gastric mucosa, classically fixed with aldehydes and osmium tetroxide. On semithin sections, somatostatin cells were identified by using the immunoperoxidase method. The ultrastructural localization of...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00375.x

    authors: Noaillac-Depeyre J

    更新日期:1985-01-01 00:00:00

  • Cytochalasin D induces changes in cell shape and promotes in vitro chondrogenesis: a morphological study.

    abstract::One of the initial events required for the expression of cartilage-specific macromolecules in monolayer cultures is the reversion to the initial round shape of chondrocytes. Thus, considerable research efforts have focused on developing reliable procedures to maintain a round morphology of cultured chondrocytes. Our s...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(96)81303-7

    authors: Loty S,Forest N,Boulekbache H,Sautier JM

    更新日期:1995-01-01 00:00:00

  • NFAT directly regulates Nkx2-5 transcription during cardiac cell differentiation.

    abstract:BACKGROUND INFORMATION:The transcription factor NFAT (nuclear factor of activated T-cell) family comprises important regulators in immuno-responses and mouse embryonic development, including early cardiovascular and heart valve development. The mechanism involved, however, is not fully understood. Nkx2-5 (NK2 transcrip...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20080108

    authors: Chen Y,Cao X

    更新日期:2009-06-01 00:00:00

  • Characterization and biological properties of L-HGP, a glycoprotein from the amphibian oviduct with acrosome-stabilizing effects.

    abstract:BACKGROUND INFORMATION:The role of the jelly coat that surrounds the amphibian oocytes has been widely discussed, but is poorly understood. The presence of the jelly coat is essential for fertilization. However, the structure and function of the molecules that comprise the jelly coat have not been thoroughly documented...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20050051

    authors: Krapf D,Vidal M,Arranz SE,Cabada MO

    更新日期:2006-07-01 00:00:00

  • Multidrug-resistance (MDR) phenotype of human osteosarcoma cells evaluated by quantitative morphological and electron microscopy analyses.

    abstract::Multidrug-resistant (MDR) variants of a human osteosarcoma cell line (U-2 OS) have been recently obtained by continuous exposure to doxorubicin (DX). The growth and phenotypic characteristics of these cell lines have been demonstrated to be related to the level of expression of P-glycoprotein. In this work, the morpho...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(96)89429-9

    authors: Zini N,Scotlandi K,Baldini N,Nini G,Sabatelli P,Maraldi N

    更新日期:1995-01-01 00:00:00

  • Sites of transcription of adenovirus type 5 genomes in relation to early viral DNA replication in infected HeLa cells. A high resolution in situ hybridization and autoradiographical study.

    abstract::The distribution of viral RNA molecules in HeLa cells infected with adenovirus type 5 (Ad5) was determined by in situ hybridization at the ultrastructural level at an intermediate stage of nuclear transformation, when viral DNA synthetic activities were maximal but progeny viruses were still sparse. Transcription site...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(91)90060-z

    authors: Puvion-Dutilleul F,Puvion E

    更新日期:1991-01-01 00:00:00

  • Efficient in vitro myogenic reprogramming of human primary mesenchymal stem cells and endothelial cells by Myf5.

    abstract:BACKGROUND INFORMATION:The identification of a source of stem cells able to regenerate skeletal muscle was the goal of numerous studies with the aim to develop new therapeutic approaches for genetic muscle diseases or muscle injuries. A series of studies have demonstrated that stem cells derived from various tissues ma...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20100112

    authors: Dimicoli-Salazar S,Bulle F,Yacia A,Massé JM,Fichelson S,Vigon I

    更新日期:2011-11-01 00:00:00

  • Focal adhesions: structure and dynamics.

    abstract::Interactions of cells with the extracellular matrix are essential for the control of tissue remodelling, cell migration, and embryogenesis. At the cell-extracellular matrix contact points, specialized structures are formed and termed focal adhesions, where transmembrane adhesion receptors provide a structural link bet...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(00)01101-1

    authors: Petit V,Thiery JP

    更新日期:2000-10-01 00:00:00

  • Recognition of xenogeneic erythrocytes: the GalNAc/Gal-particle receptor of rat liver macrophages mediates or participates in recognition.

    abstract::We studied mechanisms that mediate recognition of human erythrocytes (HRBC) and sheep erythrocytes (SRBC) by rat liver macrophages. We used an in vitro cell binding assay that allows spontaneous formation of cell contacts. Binding of HRBC to rat macrophages shows the following characteristics: inhibition studies with ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1987.tb00558.x

    authors: Mohr M,Kolb H,Kolb-Bachofen V,Schlepper-Schäfer J

    更新日期:1987-01-01 00:00:00

  • Modulations of the epithelial phenotype and functional activity of cultured bovine tracheal gland cells: dependence on the culture medium and passage number.

    abstract::Bovine tracheal gland (BTG) cells in culture show an epithelial-fibroblastoid transition after several passages. To investigate these BTG cell phenotype changes, we studied the effects of both the culture medium and passage number on the expression of epithelial cytoskeletal proteins and glandular serous cell markers....

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(91)90008-b

    authors: Benali R,Dupuit F,Chevillard M,Jacquot J,Haye B,Puchelle E

    更新日期:1991-01-01 00:00:00

  • Yeast RNA helicases of the DEAD-box family involved in translation initiation.

    abstract::RNA helicases of the DEAD-box and related families have been found to be required for all processes involving RNA molecules. Biochemical and genetic analyses have shown that at least two RNA helicases are required for translation initiation in yeast. Although it is generally believed that these enzymes are necessary t...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(03)00032-7

    authors: Linder P

    更新日期:2003-05-01 00:00:00

  • Action of photoreactive analogs of vasopressin in toad bladder.

    abstract::A series of analogs of vasopressin with photoreactive groups in positions 1, 2, 3, 4, 8 or 9 of the nonapeptide sequence have been studied for their effects on water and urea permeability of the isolated toad urinary bladder. Compounds with photoreactive groups in positions 3 or 8 bound covalently to receptors as judg...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00431.x

    authors: Eggena P,Ma CL,Fahrenholz F,Buku A,Schwartz IL

    更新日期:1985-01-01 00:00:00

  • Analysis of pore-rich areas on the nuclear envelope of spermatocytes.

    abstract::Serial sections analysis of spermatocytes of the rodent Phyllotis darwini, reveal a persistent positional relationship between nucleolar material and centrioles. Pore rich areas interposed between these nucleocytoplasmic components, are represented graphically. Centriole replication takes place during the pachytene st...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1984.tb00266.x

    authors: Couve E

    更新日期:1984-01-01 00:00:00

  • Comparative aspects of circulating erythrocytes in the trophic and reproductive phases of European eel. Ultrastructure and cytochemistry.

    abstract::Different cytochemical and morphological parameters were used to compare the functional patterns of the erythrocytes in the transition from the trophic (yellow eel) to the reproductive (silver eel) phases of European eel (Anguilla anguilla L.). Data on the nucleus were obtained by microdensitometric (Feulgen reaction)...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00402.x

    authors: Barni S,Gerzeli G

    更新日期:1985-01-01 00:00:00

  • Evolutionary aspects of peroxisomes as cell organelles, and of genes encoding peroxisomal proteins.

    abstract::Peroxisomes are present in most eukaryotic cell types, and have different enzymatic content and metabolic functions throughout the life scale. The endosymbiotic origin of these DNA-devoid organelles is supported by evolutionary data concerning genes encoding not only most peroxisomal proteins, but also several transcr...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(00)01083-2

    authors: Latruffe N,Vamecq J

    更新日期:2000-09-01 00:00:00

  • Length of the selectivity filter of aquaporin-1.

    abstract::We have characterized the selectivity filter of the water channel aquaporin-1 (AQP1) of proximal straight tubules (PST), as an equivalent cylindrical structure with a diameter of approximately 4.5 A, where water molecules single file. We report here efforts to evaluate its length. PST were dissected from rabbit kidney...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Whittembury G,González E,Gutiérrez AM,Echevarría M,Hernández CS

    更新日期:1997-08-01 00:00:00

  • Phosphoinositidase C isozymes in SaOS-2 cells: immunocytochemical detection in nuclear and cytoplasmic compartments.

    abstract::SaOS-2 cell line presents osteoblastic characteristics which can be modulated by specific agonists involving also phosphoinositide breakdown. In order to determine whether SaOS-2 cells display a phosphoinositide signalling system not only at the cytosol-cell membrane level but also, as recently reported for other cell...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(93)90143-3

    authors: Maraldi NM,Zini N,Santi S,Bavelloni A,Valmori A,Marmiroli S,Ognibene A

    更新日期:1993-01-01 00:00:00

  • Presence of aquaporin and V-ATPase on the contractile vacuole of Amoeba proteus.

    abstract:BACKGROUND INFORMATION:The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90]. RESULTS:In the present study, we cloned an AQP gene from A. proteus [A...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20070091

    authors: Nishihara E,Yokota E,Tazaki A,Orii H,Katsuhara M,Kataoka K,Igarashi H,Moriyama Y,Shimmen T,Sonobe S

    更新日期:2008-03-01 00:00:00

  • The orientation of ciliary basal bodies in quail oviduct is related to the ciliary beating cycle commencement.

    abstract::In mature ciliated cells, the basal feet associated to the basal bodies point out in the direction of the effective stroke of the ciliary beating. In contrast, during ciliogenesis, the basal feet of the newly anchored basal bodies are randomly oriented. The reorientation of basal bodies occurs during the beginning of ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00417.x

    authors: Boisvieux-Ulrich E,Laine MC,Sandoz D

    更新日期:1985-01-01 00:00:00

  • Fission yeast cytoskeletons and cell polarity factors: connecting at the cortex.

    abstract::Cell polarity is a fundamental property of cells from unicellular to multicellular organisms. Most of the time, it is essential so that the cells can achieve their function. The fission yeast Schizosaccharomyces pombe is a powerful genetic model organism for studying the molecular mechanisms of the cell polarity proce...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1042/BC20060048

    authors: La Carbona S,Le Goff C,Le Goff X

    更新日期:2006-11-01 00:00:00

  • Molecular heterogeneity of the isolated surface glycoprotein from variant AnTat 1.1 of Trypanosoma brucei brucei.

    abstract::Variant surface glycoprotein (VSG) of Trypanosoma brucei brucei AnTat 1.1 was released by means of the procedure described by Baltz et al. ([1976], Ann. Immunol. [Inst. Pasteur] 127C, 761-774). The concanavalin-A chromatography yielded 3 VSG fractions according to the addition, in the elution buffer, of alpha-methyl-D...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(88)90072-x

    authors: Boutignon F,Hublart M,Gomes V,Mendonca-Previato L,Tetaert D,Huet-Duvillier G,Degand P

    更新日期:1988-01-01 00:00:00

  • René Couteaux (1909-1999) and the morphological identification of synapses.

    abstract::During a historical research, we realized that René Couteaux (1909-1999) was the first histologist who stained the postsynaptic structure of the neuromuscular junction. By means of Janus Green B dye, he revealed the membranous 'subneural apparatus' related to the 'synaptic gutter'. Hence, he justified the use of the p...

    journal_title:Biology of the cell

    pub_type: 传,历史文章,杂志文章

    doi:10.1042/BC20050036

    authors: Tsuji S

    更新日期:2006-08-01 00:00:00

  • Mapping the cellular distribution of labelled molecules by SIMS microscopy.

    abstract::We took advantage of one of the main possibilities of ion microscopy, ie isotopic analysis, to study the cellular distribution of molecules labelled either with carbon 14 or with stable isotopes of low natural abundance such as nitrogen 15 and deuterium. The surface of the sample is bombarded with an ion beam (O2+, Cs...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(92)90011-o

    authors: Hindie E,Coulomb B,Beaupain R,Galle P

    更新日期:1992-01-01 00:00:00

  • Precursors of neuropeptides in the marine worm Nereis diversicolor. In vitro translation of a precursor related to human prepro-cholecystokinin and immunolocalization of this precursor in the nervous system.

    abstract::Total mRNA extracted from the brain of a marine worm, Nereis diversicolor, were in vitro translated using 2 cell-free systems: rabbit reticulocyte lysate and wheat germ extract. Among numerous products newly translated in both systems, only one class of 70 kDa polypeptides immunoprecipitated when we used a mixture of ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(91)90054-q

    authors: Guissi-Kadri S,Dhainaut-Courtois N,Porchet M,Curgy JJ

    更新日期:1991-01-01 00:00:00

  • Dopamine-induced programmed cell death is associated with cytochrome c release and caspase-3 activation in snail salivary gland cells.

    abstract:BACKGROUND INFORMATION:PCD (programmed cell death) is a common mechanism to remove unwanted and excessive cells from organisms. In several exocrine cell types, PCD mode of release of secretory products has been reported. The molecular mechanism of the release, however, is largely unknown. Our aim was to study the molec...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20070168

    authors: Pirger Z,Rácz B,Kiss T

    更新日期:2009-02-01 00:00:00

  • Immunodetection of lipopolysaccharide in macrophages during the processing of non invasive Shigella dysenteriae.

    abstract::The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(90)90343-2

    authors: Dacosta B,Ryter A,Mounier J,Sansonetti P

    更新日期:1990-01-01 00:00:00

  • Starved human fibroblasts secrete acidic proteins inducing post re-feeding proliferation and in vitro cell migration: a potential tool for wound healing.

    abstract:BACKGROUND INFORMATION:There are several reports indicating that starved fibroblasts show higher proliferation rates when re-fed with foetal bovine serum. We have evidence demonstrating that this phenomenon is related to secretory proteins which may be beneficial to wound healing. RESULTS:After re-feeding, 16 and 72 h...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201300063

    authors: Golpour M,Fattahi S,Niaki HA,Hadipoor A,Abedian Z,Ahangarian GR,Parsian H,Mosapour A,Khorasani HR,Vaziri HR,Bijani A,Mostafazadeh A

    更新日期:2014-05-01 00:00:00

  • Control of CREB phosphorylation and its role for induction of melatonin synthesis in rat pinealocytes.

    abstract::Phosphorylation of the transcription factor CREB appears as an important step in the signal transduction cascade that activates melatonin biosynthesis in the mammalian pineal organ. We have studied the mechanisms causing CREB phosphorylation by immunocytochemical and immunochemical demonstration of phosphorylated CREB...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(98)80006-3

    authors: Maronde E,Schomerus C,Stehle JH,Korf HW

    更新日期:1997-11-01 00:00:00

  • Endosomal recruitment of the WASH complex: active sequences and mutations impairing interaction with the retromer.

    abstract:BACKGROUND INFORMATION:The Wiskott-Aldrich syndrome protein and scar homolog (WASH) complex is the major Arp2/3 activator at the surface of endosomes. The branched actin network, that the WASH complex induces, contributes to cargo sorting and scission of transport intermediates destined for most endosomal routes. A maj...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201200038

    authors: Helfer E,Harbour ME,Henriot V,Lakisic G,Sousa-Blin C,Volceanov L,Seaman MNJ,Gautreau A

    更新日期:2013-05-01 00:00:00