Multidrug-resistance (MDR) phenotype of human osteosarcoma cells evaluated by quantitative morphological and electron microscopy analyses.

abstract：:The activity and the tissue distribution of the oxygen radical producing enzyme xanthine oxidoreductase (XOR) were measured in the digestive gland of the common marine mussel Mytilus galloprovincialis Lmk along an annual cycle. No xanthine oxidase (XOX) activity could be measured, the enzyme only displaying xanthine d...

journal_title：Biology of the cell

authors： Cancio I,Cajaraville MP

更新日期：1999-11-01 00:00:00

abstract：:Toad bladder epithelial cells were homogenized and fractionated by Percoll density-gradient centrifugation. Binding of tritium-labeled vasopressin ([3H]AVP) was measured in surface membranes (SM), microsomes (M), and a 100,000 g (60-min) microsomal supernatant fraction (S). More than two-thirds of the total receptors ...

journal_title：Biology of the cell

authors： Eggena P,Ma CL

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The rod outer segment (OS) is the specialised organelle where phototransduction takes place. Our previous proteomic and biochemical analyses on purified rod disks showed the functional expression of the respiratory chain complexes I-IV and F1 Fo -ATP synthase in OS disks, as well as active solubl...

journal_title：Biology of the cell

authors： Calzia D,Barabino S,Bianchini P,Garbarino G,Oneto M,Caicci F,Diaspro A,Tacchetti C,Manni L,Candiani S,Ravera S,Morelli A,Enrico Traverso C,Panfoli I

更新日期：2013-08-01 00:00:00

abstract：:Detergent extraction of human blood platelets pre-treated with Taxol to stabilize microtubules allows isolation of marginal band (MB) cytoskeletons. We studied MB cytoskeleton structure using dark-field light microscopy and negative stain electron microscopy (EM). Dark-field illumination clearly demonstrated the "hoop...

journal_title：Biology of the cell

authors： Kowit JD,Linck RW,Kenney DM

更新日期：1988-01-01 00:00:00

abstract：:Details of the ultrastructure of hepatitis C virus (HVC) virion remain unclear because it has proved extremely difficult to visualise virus particles from infected serum and tissues directly. In addition, although much is known about the viral genome, first cloned in 1989, little is known about HCV morphogenesis, due ...

journal_title：Biology of the cell

authors： Roingeard P,Hourioux C,Blanchard E,Brand D,Ait-Goughoulte M

更新日期：2004-03-01 00:00:00

abstract：:Separation by velocity sedimentation at unit gravity according to the STAPUT system of Miller and Phillips was applied to a population of rabbit megakaryocytes previously enriched by density gradient centrifugation. By this means, 80,000 to 100,000 megakaryocytes with 100% purity were collected in eight fractions acco...

journal_title：Biology of the cell

authors： Dupont H,Dupont MA,Bricaud H,Boisseau MR

更新日期：1983-01-01 00:00:00

abstract：:A general feature of the cell adhesion molecules belonging to the immunoglobulin family (Ig-CAMs) is to display a modular structure that provides a framework for multiple binding sites for other recognition molecules. Among this family, F3/contactin is a glycan phosphatidyl-inositol (GPI)-anchored molecule expressed b...

journal_title：Biology of the cell

authors： Falk J,Bonnon C,Girault JA,Faivre-Sarrailh C

更新日期：2002-10-01 00:00:00

abstract：:Regulated exocytosis of defensive secretory organelles, the trichocysts, as well as a transient Ca(2+)-influx can be induced in Paramecium by aminoethyldextran (Kerboeuf and Cohen, J Cell Biol (1990) 111, 2527). Knoll et al (Febs Lett (1992) 304, 265) reported that veratridine was also a secretagogue for Paramecium. H...

journal_title：Biology of the cell

authors： Kerboeuf D,Cohen J

更新日期：1996-01-01 00:00:00

abstract：:Isolated cells from the proximal tubule have previously been used to study specific renal physiological processes. Here we analysed the structural changes induced by mechanical isolation on rat proximal tubule cells. As major modifications we detected: 1) a redistribution of brush border microvilli, which spread out o...

journal_title：Biology of the cell

authors： Rebelo L,Carmo-Fonseca M,Moura TF

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND INFORMATION:CLEM (correlative live cell and electron microscopy) seeks to bridge the data acquired with different imaging strategies, typically between light microscopy and electron microscopy. It has been successfully applied in cell cultures, although its use in multicellular systems is hampered by difficu...

journal_title：Biology of the cell

authors： Kolotuev I,Schwab Y,Labouesse M

更新日期：2009-12-04 00:00:00

abstract：:Netrins are secreted proteins that play a crucial role in neuronal migration and in axon guidance during the development of the nervous system. Netrin-1 has been shown to interact with the transmembrane receptors DCC and UNC5H and these receptors appeared of key importance in mediating the chemotropic activity of netr...

journal_title：Biology of the cell

authors： Mehlen P,Mazelin L

更新日期：2003-10-01 00:00:00

abstract：:The entry into, and exit from, mitosis are controlled by a universal M-phase promoting factor (MPF) composed of at least p34cdc2 and a cyclin. Embryonic systems are convenient for studying the association and dissociation of the active MPF complex because oocytes and eggs are naturally arrested at a specific point of ...

journal_title：Biology of the cell

authors： Charbonneau M,Grandin N

更新日期：1992-01-01 00:00:00

abstract：:Our knowledge of cell signalling pathways in the molluscan immune system and their response to immunological challenge is currently poor. The present study focused on the Protein Kinase C (PKC) pathway in the immune cells (haemocytes) of Lymnaea stagnalis and its response following exposure to bacterial lipopolysaccha...

journal_title：Biology of the cell

authors： Walker AJ,Plows LD

更新日期：2003-11-01 00:00:00

abstract：:Inside-out porcine thyroid follicles in culture undergo polarity reversal after being embedded in collagen gel. The newly-formed follicles reexpress some specific thyroid functions lost in inside-out follicles (Chambard et al., 1984. We present here an ultrastructural study of the inversion of polarity in this model s...

journal_title：Biology of the cell

authors： Barriere H,Chambard M,Mauchamp J,Gabrion J

更新日期：1986-01-01 00:00:00

abstract：:Previous experiments have revealed a relatively weak electrostatic binding capacity of in vitro reconstituted intermediate filaments (IFs) as well as of natural IFs of whole cell mount preparations for purified ribosomal particles of mammalian origin. In order to demonstrate that such associations also occur in vivo, ...

journal_title：Biology of the cell

authors： Traub P,Bauer C,Hartig R,Grüb S,Stahl J

更新日期：1998-07-01 00:00:00

abstract：:Interactions of cells with the extracellular matrix are essential for the control of tissue remodelling, cell migration, and embryogenesis. At the cell-extracellular matrix contact points, specialized structures are formed and termed focal adhesions, where transmembrane adhesion receptors provide a structural link bet...

journal_title：Biology of the cell

authors： Petit V,Thiery JP

更新日期：2000-10-01 00:00:00

abstract：:Co-isolated residual nuclear shells and residual nucleoli from membrane-depleted rat liver nuclei were spread according to Kleinschmidt's method. Comparison of the spread residual structures isolated from nuclear shells and spread pore complex-lamina isolated from nuclear envelopes showed that these residual structure...

journal_title：Biology of the cell

authors： Bureau J,Hubert J,Bouteille M

更新日期：1986-01-01 00:00:00

abstract：:This study reports the synthesis of l-desamino-7-lysine- (fluorescein)-8-arginine-vasotocin (7-lys(flu) dAVT), and describes its biological activity in the isolated urinary bladder of the toad Bufo marinus. 7-lys(flu)dAVT was fully active in increasing bladder permeability to water. A half-maximal hydroosmotic respons...

journal_title：Biology of the cell

authors： Eggena P,Buku A

更新日期：1989-01-01 00:00:00

abstract：:Using gold labelled neoglycoproteins containing either alpha-D-glucose, N-acetyl-beta-D-glucosamine, alpha-D-mannose, 6-phospho-alpha-D-mannose, and alpha-L-fucose (BSA), we investigated their intranuclear binding sites in the TG human cell line. Although gold-labelled BSA did not give any noticeable labelling, the pr...

journal_title：Biology of the cell

authors： Bourgeois CA,Monsigny M

更新日期：1990-01-01 00:00:00

abstract：:The membrane content in phospholipids along the secretory pathway in rat pancreatic B-cells was studied in situ by high-resolution cytochemistry, applying the recently introduced phospholipase A2-gold technique. The gold particles were mostly associated with cell membranes, and the various types of membranes were labe...

journal_title：Biology of the cell

authors： Coulombe PA,Bendayan M

更新日期：1989-01-01 00:00:00

abstract：:The influence of triiodothyronine (T3) on avian myoblast proliferation and differentiation was studied in secondary cultures using plating densities of 2,500 and 7,000 cells/cm2. Culture media were depleted of T3 (control myoblasts) and increasing amounts were then added to concentrations of 0.6, 3 and 15 nM T3 (treat...

journal_title：Biology of the cell

authors： Marchal S,Cassar-Malek I,Pons F,Wrutniak C,Cabello G

更新日期：1993-01-01 00:00:00

abstract：:The pH-related change in morphology of vinblastine (VLB)-induced paracrystals formed in Chinese hamster ovary (CHO) cells was examined immunohistochemically in order to determine both the mechanism of tubulin crystallization and the influence of acidic pHs on cytoskeletal microtubules. Lowering the extracellular pH (p...

journal_title：Biology of the cell

authors： Takanari H,Morita J,Yamanaka H,Yada K,Takahashi A,Izutsu K

更新日期：1994-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Available data concerning the sperm morphology of teleost fishes demonstrate wide variation. In the present study, the spermatozoa of Siberian sturgeon (Acipenser baerii Brandt, 1869), a chondrostean fish, was investigated. In contrast with teleost fish, chondrostean spermatozoa have a head with ...

journal_title：Biology of the cell

authors： Psenicka M,Alavi SM,Rodina M,Gela D,Nebesarova J,Linhart O

更新日期：2007-02-01 00:00:00

abstract：BACKGROUND INFORMATION:The role of the jelly coat that surrounds the amphibian oocytes has been widely discussed, but is poorly understood. The presence of the jelly coat is essential for fertilization. However, the structure and function of the molecules that comprise the jelly coat have not been thoroughly documented...

journal_title：Biology of the cell

authors： Krapf D,Vidal M,Arranz SE,Cabada MO

更新日期：2006-07-01 00:00:00

abstract：:Immunogold labeling and biochemical methods were used to localize the site of viral protein synthesis in frog virus 3 (FV3)-infected baby hamster kidney (BHK) cells. Immunogold labeling studies of Triton-extracted (cytoskeletons), FV3-infected BHK cells with antivimentin antibodies showed that the major components of ...

journal_title：Biology of the cell

authors： Murti K,Goorha R

更新日期：1989-01-01 00:00:00

abstract：:The nucleolar lesions provoked by the action of ribonuclease (RNase) on living chick embryo fibroblasts were studied by means of microcinematographic analysis and at the ultrastructural level using oxidized diaminobenzidine as a differentially contrasting stain for nucleic acids. This study has shown that the inductio...

journal_title：Biology of the cell

authors： Chobert MN,Pouchelet M,Anteunis A,Gansmuller A,Robineaux R

更新日期：1983-01-01 00:00:00

abstract：:The lateral line of the zebrafish has many of the advantages that made the sensory organs of Drosophila a very productive model system: 1) it comprises a set of discrete sense organs (neuromasts) arranged in a defined, species-specific pattern, such that each organ can be individually recognized; 2) the neuromasts are...

journal_title：Biology of the cell

authors： Dambly-Chaudière C,Sapède D,Soubiran F,Decorde K,Gompel N,Ghysen A

更新日期：2003-12-01 00:00:00

abstract：:During a historical research, we realized that René Couteaux (1909-1999) was the first histologist who stained the postsynaptic structure of the neuromuscular junction. By means of Janus Green B dye, he revealed the membranous 'subneural apparatus' related to the 'synaptic gutter'. Hence, he justified the use of the p...

journal_title：Biology of the cell

authors： Tsuji S

更新日期：2006-08-01 00:00:00

abstract：:The internalization of the yolk proteins has been investigated by electron microscopy and cytochemistry in the oocyte of the trout which stores up large quantities of yolk. The oocyte evolution has been followed for 18 months in a homogeneous group of animals. Anionic ferritin has been injected during vitellogenesis. ...

journal_title：Biology of the cell

authors： Busson-Mabillot S

更新日期：1984-01-01 00:00:00

abstract：:During wound-healing in cultured frog skin fragments, fibronectin (FN) was detected in the dermal-epidermal junction. Intracellular fibronectin was stained using permeabilization and DAB immunoperoxidase. With electron microscopy intracytoplasmic FN granules were localized in the epidermal processes of the stratum ger...

journal_title：Biology of the cell

authors： Denefle JP,Zhu QL,Lechaire JP

更新日期：1989-01-01 00:00:00