Abstract：
:Immunogold labeling and biochemical methods were used to localize the site of viral protein synthesis in frog virus 3 (FV3)-infected baby hamster kidney (BHK) cells. Immunogold labeling studies of Triton-extracted (cytoskeletons), FV3-infected BHK cells with antivimentin antibodies showed that the major components of the detergent-resistant residue are the intermediate filaments (IF) and polyribosomes. Double immunogold labeling studies with anti-FV3 and antivimentin antibodies revealed that FV3 proteins are associated with polyribosomes that are bound to the IF network. Biochemical studies of the cytoskeletons prepared from FV3-infected cells showed that a substantial fraction of all newly synthesized FV3 proteins associate with the cytoskeleton and that the association is not disrupted by colchicine (microtubule-disrupting drug) or cytochalasin B (microfilament-disrupting drug). Together the above studies suggest that IF may provide the scaffold for the attachment of polyribosomes that are active in viral protein synthesis.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Murti K,Goorha Rsubject
Has Abstractpub_date
1989-01-01 00:00:00pages
205-14issue
3eissn
0248-4900issn
1768-322Xpii
0248-4900(89)90030-0journal_volume
65pub_type
杂志文章abstract：:Arabinoxylans were localised by immunocytochemistry using polyclonal antibodies in the cell walls of the apical internode of maize after degradation in the rumen. In order to understand the significance of arabinoxylan in digestibility property, two lines of maize differing in digestibility were used. Wide variations ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1996.tb00988.x
更新日期：1996-01-01 00:00:00
abstract：:We studied mechanisms that mediate recognition of human erythrocytes (HRBC) and sheep erythrocytes (SRBC) by rat liver macrophages. We used an in vitro cell binding assay that allows spontaneous formation of cell contacts. Binding of HRBC to rat macrophages shows the following characteristics: inhibition studies with ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00558.x
更新日期：1987-01-01 00:00:00
abstract：BACKGROUND INFORMATION:The rod outer segment (OS) is the specialised organelle where phototransduction takes place. Our previous proteomic and biochemical analyses on purified rod disks showed the functional expression of the respiratory chain complexes I-IV and F1 Fo -ATP synthase in OS disks, as well as active solubl...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201300003
更新日期：2013-08-01 00:00:00
abstract：:Much remains to be learned about how cancer cells acquire the property of migration, a prerequisite for invasiveness and metastasis. Loss of p53 functions is assumed to be a crucial step in the development of many types of cancers, leading to dysregulation of cell cycle checkpoint controls and apoptosis. However, emer...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20050058
更新日期：2006-03-01 00:00:00
abstract：:Ultrastructural morphometry (point-counting method) was used to evaluate stereological parameters of rough endoplasmic reticulum (RER), Golgi complex, secretory granules, mitochondria and lysosomes of Rana ridibunda pituitary corticotropic (ACTH) cells in animals subjected to constant environmental conditions (CEC) in...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00472.x
更新日期：1986-01-01 00:00:00
abstract：:We used a high-resolution STEM to attempt to recognize the spatial localization of the macromolecular components of ribosomes isolated from Tetrahymena pyriformis. Images obtained either by the annular dark field detector or by the ratio-contrast mode (Z-contrast) show a ribosome as constituted of rounded dark areas, ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00271.x
更新日期：1984-01-01 00:00:00
abstract：:A history of the early development of the confocal laser scanning microscope in the MRC Laboratory of Molecular Biology in Cambridge is presented. The rapid uptake of this technology is explained by the wide use of fluorescence in the 80s. The key innovations were the scanning of the light beam over the specimen rathe...
journal_title：Biology of the cell
pub_type： 历史文章,杂志文章,评审
doi：10.1016/s0248-4900(03)00078-9
更新日期：2003-09-01 00:00:00
abstract：:The sensory organs of the vertebrate head derive from two embryological structures, the neural crest and the ectodermal placodes. Although quite a lot is known about the secreted and transcription factors that regulate neural crest development, until recently little was known about the molecular pathways that regulate...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20040515
更新日期：2005-05-01 00:00:00
abstract：:Antidiuretic hormone induces, in the apical plasma membrane of amphibian urinary bladder epithelial cells, the exocytotic insertion of intramembranous particle aggregates that probably contain water channels. Purification of the apical membrane is a way to characterize the aggregates. The isolation of such purified me...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Revision of the reference map of the polytene chromosomes of Drosophila subobscura was started by means of the electron microscope. We present a map of regions 70B to 72D of the E chromosome obtained from squashed and thin-sectioned salivary glands. It was observed that the total number of bands in divisions 70B to 72...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1988.tb00750.x
更新日期：1988-01-01 00:00:00
abstract：:cdc25 proteins are universally involved in the control of cell division. Using an original method of sequence analysis, cdc25 proteins from different sources were compared to protein phosphatases. Protein phosphatases could clearly be characterized as two distinct protein families, the phospho-seryl/threonyl phosphata...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90134-m
更新日期：1992-01-01 00:00:00
abstract：:The mammalian spermatozoa are endowed with a unique cytoskeleton which consists both of ubiquitous and specific proteins, some of them arising from gene haploid transcription. In the head, a dense perinuclear layer is made of basic proteins (calicin, cylicin, etc) associated with calmodulin and actin remnants. In the ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(94)80001-4
更新日期：1994-01-01 00:00:00
abstract：BACKGROUND:Integrins are transmembrane alphabeta heterodimer receptors that function as structural and functional bridges between the cytoskeleton and ECM (extracellular matrix) molecules. The RGD (arginine-glycine-aspartate tripeptide motif)-dependent integrin alpha8beta1 has been shown to be involved in various cell ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20090060
更新日期：2009-09-14 00:00:00
abstract：:This study was undertaken to evaluate whether hypertrophy and hyperplasia of the pancreatic acinar cells induced by caerulein remained after termination of the hormonal treatment. Rats received subcutaneous injections of saline or caerulein for 4 days and were killed immediately after termination of treatment or 2, 15...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Genetic studies in the budding yeast have led to the molecular characterization of gamma-tubulin associated proteins and to the identification of orthologues in animal cells. While the gamma-tubulin complex is more complex in animal cells than in budding yeast, its function is probably maintained throughout evolution....
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1999-05-01 00:00:00
abstract：:Immunofluorescent techniques have been used in the analysis of DNA-RNA hybrids occurrence and its relationship to transcriptional events on polytene chromosomes of Drosophila subobscura. We have studied the distribution of these hybrids on uninduced/induced chromosomes. Two different indirect immunofluorescence method...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90140-v
更新日期：1992-01-01 00:00:00
abstract：:We have identified and characterized a membrane glycoprotein located at the apical plasma membrane of adult human colon epithelial cells, by the use of the monoclonal antibody technique in combination with immunocytochemical and biochemical methods. Analysis of membranes extracted with Triton X-114 and treated with sp...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00557.x
更新日期：1987-01-01 00:00:00
abstract：:Using gold labelled neoglycoproteins containing either alpha-D-glucose, N-acetyl-beta-D-glucosamine, alpha-D-mannose, 6-phospho-alpha-D-mannose, and alpha-L-fucose (BSA), we investigated their intranuclear binding sites in the TG human cell line. Although gold-labelled BSA did not give any noticeable labelling, the pr...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1990.tb00007.x
更新日期：1990-01-01 00:00:00
abstract：:The ciliated protozoan Paramecium harbours a particularly large diversity of microtubule networks, ranging from the elaborate and stable ciliary axonemes and basal bodies to very dynamic cytoplasmic, cortical or intranuclear arrays. Their organization and individual cycle of assembly/disassembly are well known and ext...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(97)89840-1
更新日期：1996-01-01 00:00:00
abstract：:Actin- and microtubule-based motors can propel different cargos along filaments. Within cells, they control the distribution of membrane-bound compartments by performing complementary tasks. Organelles make long journeys along microtubules, with class V myosins ensuring their capture and their dispersal in actin-rich ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20070021
更新日期：2007-08-01 00:00:00
abstract：BACKGROUND INFORMATION:MTA1 (metastasis-associated gene 1) has been reported to be overexpressed in cancers with high potential to metastasize. Studies of the molecular mechanisms revealed that MTA1 plays an important role in the process of metastasis of many types of cancer. However, the role of MTA1 in melanoma devel...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/bc20060130
更新日期：2007-10-01 00:00:00
abstract：:Details of the ultrastructure of hepatitis C virus (HVC) virion remain unclear because it has proved extremely difficult to visualise virus particles from infected serum and tissues directly. In addition, although much is known about the viral genome, first cloned in 1989, little is known about HCV morphogenesis, due ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/j.biolcel.2003.11.006
更新日期：2004-03-01 00:00:00
abstract：:Isolated cells from the proximal tubule have previously been used to study specific renal physiological processes. Here we analysed the structural changes induced by mechanical isolation on rat proximal tubule cells. As major modifications we detected: 1) a redistribution of brush border microvilli, which spread out o...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90026-w
更新日期：1992-01-01 00:00:00
abstract：:Image cytometry was applied to study the intracellular localization of autofluorescence and the influence of an oxidative stress on this emission. K562 erythroleukemia cancer cells were analyzed with a microspectrofluorometer, coupled with a Argon laser (Ar+) (363 nm). From each cell, 15 x 15 emission spectra were rec...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(01)01135-2
更新日期：2001-11-01 00:00:00
abstract：:Changes in the organization of centrosomes in mouse splenic T lymphocytes stimulated by concanavalin A (con A) were examined by electron microscopy of serial sections. In both resting and stimulated lymphocytes the single centrosome consists of a pair of centrioles, satellite bodies, and pericentriolar material. In re...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1985.tb00332.x
更新日期：1984-01-01 00:00:00
abstract：:The dodecasatellite is a 11/12 bp tandemly repeated sequence which is overrepresented, with regard to its genomic representation, in extrachromosomal circular DNAs from D melanogaster embryos. Here we show that a bacterial plasmid carrying a cluster of dodecasatellite is not able to replicate efficiently in cultured c...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(93)90262-d
更新日期：1993-01-01 00:00:00
abstract：:The activation of M-phase promoting factor (MPF) in one-cell mouse embryo is independent from the nucleus. Other autonomous phenomena include the cortical activity observed at the end of the first cell cycle and the reorganization of the microtubule network. Here, we observed that the autonomous control of MPF activat...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:Somatostatin-immunoreactive cells were localized on semithin and ultrathin sections of Epon-embedded samples of perch gastric mucosa, classically fixed with aldehydes and osmium tetroxide. On semithin sections, somatostatin cells were identified by using the immunoperoxidase method. The ultrastructural localization of...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1985.tb00375.x
更新日期：1985-01-01 00:00:00
abstract：:Retinitis pigmentosa (RP) is a heterogeneous group of inherited retinal degenerative diseases, characterized by the progressive death of rod and cone photoreceptors. A tremendous genetic heterogeneity is associated with the RP phenotype. Most mutations affect rods selectively and, through an unknown pathway, cause the...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/j.biolcel.2004.01.006
更新日期：2004-05-01 00:00:00
abstract：:The quick-freeze, freeze-fracture, deep-etching and rotary replication techniques were used to analyze the structural organization of Giardia duodenalis. The surface of the flagella was rugous, in contrast to the cell body surface which was smooth. The ventral region was characterized in more detail, exposing the laye...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(96)84780-0
更新日期：1996-01-01 00:00:00
