Abstract:
:Image cytometry was applied to study the intracellular localization of autofluorescence and the influence of an oxidative stress on this emission. K562 erythroleukemia cancer cells were analyzed with a microspectrofluorometer, coupled with a Argon laser (Ar+) (363 nm). From each cell, 15 x 15 emission spectra were recorded in the 400-600 nm spectral range to generate a spectral image of autofluorescence. The intracellular locations of the autofluorescence emission and of the specific mitochondrial probe rhodamine 123 (R123) were matched. Under a 363 nm excitation, all spectra from K562 cells show equivalent profiles with a 455 nm maximum emission, near of reduced nicotinamide adenine dinucleotide-(Phosphate) solution (NAD(P)H) (465 nm maximum emission). The spatial distribution of autofluorescence is homogeneous and different from the one of R123. Hydrogen peroxide (H2O2) (200 microM) and menadione (Men) (5 microM) induce a weak spectral change and a decrease in autofluorescence intensity, down to 40% of the initial emission. Doxorubicin (Dox) induces a dose-dependent decrease in autofluorescence emission and a release of intracellular free radicals. When cells were pre-treated 1 h with 1 mM glutathione (GSH), Dox induces a lower free radicals release, no significant variation of autofluorescence intensity and a lower growth inhibitory effect. Images cytometry of autofluorescence suggest that the intracellular NAD(P)H would not be restricted to mitochondrial compartments. The release of free radicals was associated with a decrease in autofluorescence intensity, mainly attributed to NAD(P)H oxidation both inside and outside mitochondria.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Bondza-Kibangou P,Millot C,Dufer J,Millot JMdoi
10.1016/s0248-4900(01)01135-2subject
Has Abstractpub_date
2001-11-01 00:00:00pages
273-80issue
5eissn
0248-4900issn
1768-322Xpii
S0248-4900(01)01135-2journal_volume
93pub_type
杂志文章abstract::The entry into, and exit from, mitosis are controlled by a universal M-phase promoting factor (MPF) composed of at least p34cdc2 and a cyclin. Embryonic systems are convenient for studying the association and dissociation of the active MPF complex because oocytes and eggs are naturally arrested at a specific point of ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/0248-4900(92)90137-p
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journal_title:Biology of the cell
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更新日期:2005-09-01 00:00:00
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journal_title:Biology of the cell
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更新日期:1993-01-01 00:00:00
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journal_title:Biology of the cell
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更新日期:1988-01-01 00:00:00
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journal_title:Biology of the cell
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更新日期:1986-01-01 00:00:00
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(92)90016-t
更新日期:1992-01-01 00:00:00
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journal_title:Biology of the cell
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更新日期:1988-01-01 00:00:00
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journal_title:Biology of the cell
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更新日期:2012-08-01 00:00:00
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journal_title:Biology of the cell
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doi:10.1016/s0248-4900(01)01126-1
更新日期:2001-09-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20040515
更新日期:2005-05-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(94)80004-9
更新日期:1994-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1111/j.1768-322x.1993.tb00899.x
更新日期:1993-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20060068
更新日期:2007-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
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journal_title:Biology of the cell
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doi:10.1111/boc.201300020
更新日期:2013-08-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(92)90441-3
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journal_title:Biology of the cell
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更新日期:2000-08-01 00:00:00
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journal_title:Biology of the cell
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更新日期:2011-11-01 00:00:00
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journal_title:Biology of the cell
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doi:
更新日期:1989-01-01 00:00:00
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journal_title:Biology of the cell
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doi:10.1042/BC20100145
更新日期:2011-08-01 00:00:00
abstract::The ciliated protozoan Paramecium harbours a particularly large diversity of microtubule networks, ranging from the elaborate and stable ciliary axonemes and basal bodies to very dynamic cytoplasmic, cortical or intranuclear arrays. Their organization and individual cycle of assembly/disassembly are well known and ext...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(97)89840-1
更新日期:1996-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20100112
更新日期:2011-11-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00487.x
更新日期:1986-01-01 00:00:00
abstract::The isolation, characterization, and expression of a novel cDNA encoding a Trypanosoma cruzi polypeptide (TcAc2), homologous to various small stress proteins and glutathione S-transferases, are described. The deduced amino-acid sequence revealed two domains sharing 27% identity and an additional 27% similarity to each...
journal_title:Biology of the cell
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doi:10.1016/0248-4900(94)90011-6
更新日期:1994-01-01 00:00:00