Hepatitis C virus ultrastructure and morphogenesis.

Abstract:

:Details of the ultrastructure of hepatitis C virus (HVC) virion remain unclear because it has proved extremely difficult to visualise virus particles from infected serum and tissues directly. In addition, although much is known about the viral genome, first cloned in 1989, little is known about HCV morphogenesis, due to the lack of an efficient in vitro culture system for HCV propagation. Virus-like particles (VLPs) obtained by expressing genes encoding the HCV structural proteins in mammalian cells can be used as an alternative model for studying HCV morphogenesis. In particular, this HCV-LP model has made it possible to demonstrate that HCV budding occurs at the ER membrane and that the core protein drives this process. The HCV-LP model opens up new possibilities for the investigation of viral morphogenesis and virus-host cell interactions, which may make it possible to establish the long-awaited in vitro culture system for HCV.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Roingeard P,Hourioux C,Blanchard E,Brand D,Ait-Goughoulte M

doi

10.1016/j.biolcel.2003.11.006

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

103-8

issue

2

eissn

0248-4900

issn

1768-322X

journal_volume

96

pub_type

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