Abstract：
BACKGROUND INFORMATION:The assembly and maintenance of cilia depend on IFT (intraflagellar transport) mediated by molecular motors and their interplay with IFT proteins. Here, we have analysed the involvement of IFT proteins in the ciliogenesis of mammalian photoreceptor cilia. RESULTS:Electron microscopy revealed that ciliogenesis in mouse photoreceptor cells follows an intracellular ciliogenesis pathway, divided into six distinct stages. The first stages are characterized by electron-dense centriolar satellites and a ciliary vesicle, whereas the formations of the ciliary shaft and the light-sensitive outer segment discs are features of the later stages. IFT proteins were associated with ciliary apparatus during all stages of photoreceptor cell development. CONCLUSIONS:Our data conclusively provide evidence for the participation of IFT proteins in photoreceptor cell ciliogenesis, including the formation of the ciliary vesicle and the elongation of the primary cilium. In advanced stages of ciliogenesis the ciliary localization of IFT proteins indicates a role in IFT as is seen in mature cilia. A prominent accumulation of IFT proteins in the periciliary cytoplasm at the base of the cilia in these stages most probably resembles a reserve pool of IFT molecules for further delivery into the growing ciliary shaft and their subsequent function in IFT. Nevertheless, the cytoplasmic localization of IFT proteins in the absence of a ciliary shaft in early stages of ciliogenesis may indicate roles of IFT proteins beyond their well-established function for IFT in mature cilia and flagella.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Sedmak T,Wolfrum Udoi
10.1042/BC20110034subject
Has Abstractpub_date
2011-10-01 00:00:00pages
449-66issue
10eissn
0248-4900issn
1768-322Xpii
BC20110034journal_volume
103pub_type
杂志文章abstract：:The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90343-2
更新日期：1990-01-01 00:00:00
abstract：:Human pancreatic ductal cells of the Capan 1 cell line differentiate progressively during growth. After the exponential growth phase, the cells elongate and become polarized with their apical poles covered by microvilli and separated from the basolateral pole by tight junctions. In this stationary phase, they form dom...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(94)80004-9
更新日期：1994-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Death receptors (DRs) induce intracellular signalling upon engagement of their cognate ligands, leading to apoptosis, cell survival or pro-inflammatory responses. In mammals, DR signalling is mediated by the recruitment of several DD (death domain)-containing molecules, such as FADD (Fas-associat...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20050091
更新日期：2006-08-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(02)01185-1
更新日期：2002-05-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00266.x
更新日期：1984-01-01 00:00:00
abstract：:Phosphorylation of the transcription factor CREB appears as an important step in the signal transduction cascade that activates melatonin biosynthesis in the mammalian pineal organ. We have studied the mechanisms causing CREB phosphorylation by immunocytochemical and immunochemical demonstration of phosphorylated CREB...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(98)80006-3
更新日期：1997-11-01 00:00:00
abstract：:Immunogold labeling and biochemical methods were used to localize the site of viral protein synthesis in frog virus 3 (FV3)-infected baby hamster kidney (BHK) cells. Immunogold labeling studies of Triton-extracted (cytoskeletons), FV3-infected BHK cells with antivimentin antibodies showed that the major components of ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(00)01101-1
更新日期：2000-10-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The first step of polyamine uptake is the binding of polyamines to the cell membrane. In order to characterize the specificity of the putrescine binding sites at the surface of the glioblastoma cells (U251), we have carried out competition experiments between putrescine bound to latex microspheres and vizualized by sc...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90369-e
更新日期：1990-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201200075
更新日期：2013-07-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00539.x
更新日期：1987-01-01 00:00:00
abstract：:The activation of M-phase promoting factor (MPF) in one-cell mouse embryo is independent from the nucleus. Other autonomous phenomena include the cortical activity observed at the end of the first cell cycle and the reorganization of the microtubule network. Here, we observed that the autonomous control of MPF activat...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(91)90069-y
更新日期：1991-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20080164
更新日期：2009-06-04 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.201400063
更新日期：2015-05-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00297.x
更新日期：1984-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1998-07-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(93)90143-3
更新日期：1993-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1997-08-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Vertebrate embryos are able to reconstitute the body plan when early blastomeres are deleted, but it is not known whether this is accomplished by cells local to the lesion or by a readjustment of the entire pattern of the embryo. We distinguished between these two possibilities by studying which embryonic cells change...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/j.biolcel.2004.06.002
更新日期：2004-09-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00494.x
更新日期：1986-01-01 00:00:00
abstract：:RNA helicases of the DEAD-box and related families have been found to be required for all processes involving RNA molecules. Biochemical and genetic analyses have shown that at least two RNA helicases are required for translation initiation in yeast. Although it is generally believed that these enzymes are necessary t...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(03)00032-7
更新日期：2003-05-01 00:00:00
abstract：BACKGROUND INFORMATION:The BOR (branchio-oto-renal) syndrome is a dominant disorder most commonly caused by mutations in the EYA1 (Eyes Absent 1) gene. Symptoms commonly include deafness and renal anomalies. RESULTS:We have used the embryos of the frog Xenopus laevis as an animal model for early ear development to exa...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20090098
更新日期：2010-02-17 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(96)85279-8
更新日期：1995-01-01 00:00:00
abstract：:A series of analogs of vasopressin with photoreactive groups in positions 1, 2, 3, 4, 8 or 9 of the nonapeptide sequence have been studied for their effects on water and urea permeability of the isolated toad urinary bladder. Compounds with photoreactive groups in positions 3 or 8 bound covalently to receptors as judg...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1985.tb00431.x
更新日期：1985-01-01 00:00:00
abstract：:One of the initial events required for the expression of cartilage-specific macromolecules in monolayer cultures is the reversion to the initial round shape of chondrocytes. Thus, considerable research efforts have focused on developing reliable procedures to maintain a round morphology of cultured chondrocytes. Our s...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(96)81303-7
更新日期：1995-01-01 00:00:00
