Abstract:
BACKGROUND INFORMATION:The assembly and maintenance of cilia depend on IFT (intraflagellar transport) mediated by molecular motors and their interplay with IFT proteins. Here, we have analysed the involvement of IFT proteins in the ciliogenesis of mammalian photoreceptor cilia. RESULTS:Electron microscopy revealed that ciliogenesis in mouse photoreceptor cells follows an intracellular ciliogenesis pathway, divided into six distinct stages. The first stages are characterized by electron-dense centriolar satellites and a ciliary vesicle, whereas the formations of the ciliary shaft and the light-sensitive outer segment discs are features of the later stages. IFT proteins were associated with ciliary apparatus during all stages of photoreceptor cell development. CONCLUSIONS:Our data conclusively provide evidence for the participation of IFT proteins in photoreceptor cell ciliogenesis, including the formation of the ciliary vesicle and the elongation of the primary cilium. In advanced stages of ciliogenesis the ciliary localization of IFT proteins indicates a role in IFT as is seen in mature cilia. A prominent accumulation of IFT proteins in the periciliary cytoplasm at the base of the cilia in these stages most probably resembles a reserve pool of IFT molecules for further delivery into the growing ciliary shaft and their subsequent function in IFT. Nevertheless, the cytoplasmic localization of IFT proteins in the absence of a ciliary shaft in early stages of ciliogenesis may indicate roles of IFT proteins beyond their well-established function for IFT in mature cilia and flagella.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Sedmak T,Wolfrum Udoi
10.1042/BC20110034subject
Has Abstractpub_date
2011-10-01 00:00:00pages
449-66issue
10eissn
0248-4900issn
1768-322Xpii
BC20110034journal_volume
103pub_type
杂志文章abstract::The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90343-2
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
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更新日期:1989-01-01 00:00:00
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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更新日期:1987-01-01 00:00:00
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journal_title:Biology of the cell
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doi:
更新日期:1998-11-01 00:00:00
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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doi:
更新日期:1989-01-01 00:00:00
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journal_title:Biology of the cell
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更新日期:2009-06-04 00:00:00
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章,评审
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更新日期:2003-05-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20090098
更新日期:2010-02-17 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(96)81303-7
更新日期:1995-01-01 00:00:00