Inhibition of the GTPase dynamin or actin depolymerisation initiates outward plasma membrane tubulation/vesiculation (cytoneme formation) in neutrophils.

Abstract:

BACKGROUND INFORMATION:In a previous study, we demonstrated that human neutrophils can develop membrane tubulovesicular extensions (TVEs) that are 160-250 nm in width and several micrometres long. These extensions, or cytonemes, are capable of establishing long-range contacts with other cells or bacteria. Cytonemes consist of membrane tubules and vesicles of a uniform diameter aligned in a row. The mechanism of membrane tubulation/vesiculation to form cytonemes remains unknown. Upon endocytosis, the GTPase dynamin and an intact actin cytoskeleton are required for endocytic vesicles scission from the plasma membrane. RESULTS:We examined the effects of dynasore (a dynamin specific inhibitor), and of cytochalasin D and latrunculin A (actin cytoskeleton disruption agents), on cytoneme formation in neutrophils. Scanning and transmission electron microscopy were used to observe cytoneme formation. High-performance chromatography and mass spectrometry were used to estimate the protein composition of the cytonemes. In neutrophils, dynasore and cytochalasin D or latrunculin A initiated the formation of tubular cytonemes that were similar in diameter and composition. The formation of cytonemes in cells treated with cytochalasin D was accompanied by the appearance of tubular invaginations of the same diameter on the plasma membrane of neutrophils. The formation of dynasore- or cytochalasin D-induced cytonemes, however, was blocked by the nitric oxide (NO) synthases inhibitor l-NAME, indicating that NO is involved in cytoneme development. Proteome analysis indicated that dynasore- or cytochalasin D-induced cytonemes are secretory protrusions that contain neutrophil bactericides along with cytoplasmic proteins, such as glycolytic enzymes and actin cytoskeleton components. CONCLUSIONS:Inhibition of dynamin with dynasore or actin depolymerisation with cytochalasin D or latrunculin A might impair the membrane fusion/fission events that are required for the separation of secretory vesicles from the plasma membrane and from each other. As a result, the secretory process extends from the cells as membrane TVEs or cytonemes. Modification of secretion gives neutrophils the possibility to communicate with other cells over distance via highly adhesive cellular secretory protrusions (cytonemes). Cytonemes deliver their membrane-packed content exactly to the destination without dilution and without harm to the surrounding tissues.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Galkina SI,Fedorova NV,Serebryakova MV,Arifulin EA,Stadnichuk VI,Gaponova TV,Baratova LA,Sud'ina GF

doi

10.1111/boc.201400063

subject

Has Abstract

pub_date

2015-05-01 00:00:00

pages

144-58

issue

5

eissn

0248-4900

issn

1768-322X

journal_volume

107

pub_type

杂志文章
  • Yeast RNA helicases of the DEAD-box family involved in translation initiation.

    abstract::RNA helicases of the DEAD-box and related families have been found to be required for all processes involving RNA molecules. Biochemical and genetic analyses have shown that at least two RNA helicases are required for translation initiation in yeast. Although it is generally believed that these enzymes are necessary t...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(03)00032-7

    authors: Linder P

    更新日期:2003-05-01 00:00:00

  • Dermal tracts in frog skin: fibronectin pathways for cell migration.

    abstract::The dermis of the frog skin (Rana esculenta) displayed a remarkable organization of vertical and horizontal tracts. Vertical thick tracts connected the dermal Stratum spongiosum with the subcutaneous tissue. Horizontal thin tracts were found alongside and contiguous to them. The thick tracts were sheathed by collagen ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1987.tb00533.x

    authors: Denèfle JP,Zhu QL,Lechaire JP

    更新日期:1987-01-01 00:00:00

  • Stimulation by aldosterone of a conductive chloride pathway in toad skin.

    abstract::As a rule, chloride movement (JC1-) across amphibian skin is considered to be passive; this is implied in fact for preparations incubated in Ringer's fluid, since short-circuit current (Isc) is the quantitative expression of net, active sodium transport (JNa+). The nature of the Cl- pathway(s) was investigated by incu...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Crabbe J,Beaujean V,Devuyst O

    更新日期:1989-01-01 00:00:00

  • TPA induces apoptosis in MPC-11 mouse plasmacytoma cells grown in serum-free medium.

    abstract::Apoptotic-like events could be rapidly induced by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in cells of the mouse plasmacytoma cell line MPC-11 grown in serum-free medium. Indicators for apoptosis were morphological changes visualized by light and electron microscopy, such as chromatin condensation ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Giese G,Schmidt J,Gilbert M,Albrecht R,Traub P

    更新日期:1997-05-01 00:00:00

  • Characterisation of endogenous Claudin-1 expression, motility and susceptibility to hepatitis C virus in CRISPR knock-in cells.

    abstract:BACKGROUND INFORMATION:Claudin-1 (CLDN1) is a four-span transmembrane protein localised at cell-cell tight junctions (TJs), playing an important role in epithelial impermeability and tissue homoeostasis under physiological conditions. Moreover, CLDN1 expression is up-regulated in several cancers, and the level of CLDN1...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201900085

    authors: Clément CMH,Deffieu MS,Dorobantu CM,Baumert TF,Ayala-Nunez NV,Mély Y,Ronde P,Gaudin R

    更新日期:2020-05-01 00:00:00

  • The plasma membrane of Entamoeba histolytica: structure and dynamics.

    abstract::The plasma membrane components of the parasitic protozoan Entamoeba histolytica, the causative agent of human invasive amebiasis, have been biochemically and immunologically characterized during the last decade. In addition, genes coding for certain surface proteins have been cloned. In spite of these advances, a unif...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1111/j.1768-322x.1991.tb03015.x

    authors: Espinosa-Cantellano M,Martínez-Palomo A

    更新日期:1991-01-01 00:00:00

  • High molecular weight microtubule-associated proteins from pig brain are immunologically related to human erythrocyte membrane proteins spectrin, ankyrin, proteins 4.1 and 4.2.

    abstract::The microtubule-associated proteins MAPs 1 and 2 from pig brain have been found to react with antibodies directed against human ankyrin and spectrin, respectively (Bennett and Davis, 1981; Davis and Bennett, 1982). In a complementary approach we have prepared antibodies against MAP1 alpha. MAP1 gamma and MAP2 purified...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00361.x

    authors: Hill AM,Cassoly R,Chetrite G,Pantaloni D

    更新日期:1985-01-01 00:00:00

  • Acute changes in temperature or oxygen availability induce ROS fluctuations in Daphnia magna linked with fluctuations of reduced and oxidized glutathione, catalase activity and gene (haemoglobin) expression.

    abstract:BACKGROUND INFORMATION:ROS (reactive oxygen species) as well as components of the antioxidant redox systems may act as signals. To link acute environmental change with gene expression, changes in ROS and GSH/GSSG (reduced/oxidized glutathione) level were measured upon acute changes in temperature or oxygen availability...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20100145

    authors: Becker D,Brinkmann BF,Zeis B,Paul RJ

    更新日期:2011-08-01 00:00:00

  • Once upon a time there was beta-catenin in cadherin-mediated signalling.

    abstract::beta-Catenin was initially characterized as a protein interacting with the cadherin cytoplasmic tail and regulating cell-cell contacts and actin cytoskeleton interactions. Moreover, the gene coding for the Drosophila orthologue of beta-catenin, armadillo, was independently identified downstream of wingless in the segm...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20040531

    authors: Gavard J,Mège RM

    更新日期:2005-12-01 00:00:00

  • Relationship between microtubules and Golgi apparatus in hepatocytes: a quantitative study during experimental nephrosis.

    abstract::In many cell types, microtubules are preferentially associated with the Golgi apparatus. However, the existence of a functional link between these two organelles is still hypothetical. To gain insight into this question, the relationships between microtubules and the Golgi apparatus were studied in rat hepatocytes dur...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1984.tb00222.x

    authors: Maurice M,Bellon B,Mahu JL,Feldmann G

    更新日期:1983-01-01 00:00:00

  • Possible role of cortactin phosphorylation by protein kinase Cα in actin-bundle formation at growth cone.

    abstract:BACKGROUND INFORMATION:Cortactin contributes to growth cone morphogenesis by forming with dynamin, ring-shaped complexes that mechanically bundle and stabilise F-actin. However, the regulatory mechanism of cortactin action is poorly understood. RESULTS:Immunofluorescence microscopy revealed that protein kinase C (PKC)...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201500032

    authors: Yamada H,Kikuchi T,Masumoto T,Wei FY,Abe T,Takeda T,Nishiki T,Tomizawa K,Watanabe M,Matsui H,Takei K

    更新日期:2015-09-01 00:00:00

  • Flow cytometry's contribution to the measurement of cell functions.

    abstract::Flow cytometry has important advantages over conventional techniques. It is rapid, highly sensitive and allows multi-parametric analysis and cell sorting. Potential exists for the measurement of many cell functions by flow cytometry. The technique can be used to determine cell viability, intracellular calcium and pH, ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/0248-4900(93)90119-y

    authors: Maftah A,Huet O,Gallet PF,Ratinaud MH

    更新日期:1993-01-01 00:00:00

  • Chromosomal localization of the mouse gene coding for the 68 kDa neurofilament subunit.

    abstract::The chromosomal localization of the mouse gene coding for the 68 kDa intermediate filament subunit of neurones (NF-L) was determined by in situ hybridization using specific 3H-labelled DNA probes. There is only one copy of the NF-L gene. The gene encoding NF-L is located on chromosome 14 region (D1-E1). ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1989.tb00867.x

    authors: Mattei MG,Duprey P,Li ZL,Mattei JF,Paulin D

    更新日期:1989-01-01 00:00:00

  • Precursors of neuropeptides in the marine worm Nereis diversicolor. In vitro translation of a precursor related to human prepro-cholecystokinin and immunolocalization of this precursor in the nervous system.

    abstract::Total mRNA extracted from the brain of a marine worm, Nereis diversicolor, were in vitro translated using 2 cell-free systems: rabbit reticulocyte lysate and wheat germ extract. Among numerous products newly translated in both systems, only one class of 70 kDa polypeptides immunoprecipitated when we used a mixture of ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(91)90054-q

    authors: Guissi-Kadri S,Dhainaut-Courtois N,Porchet M,Curgy JJ

    更新日期:1991-01-01 00:00:00

  • Hepatitis C virus ultrastructure and morphogenesis.

    abstract::Details of the ultrastructure of hepatitis C virus (HVC) virion remain unclear because it has proved extremely difficult to visualise virus particles from infected serum and tissues directly. In addition, although much is known about the viral genome, first cloned in 1989, little is known about HCV morphogenesis, due ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.biolcel.2003.11.006

    authors: Roingeard P,Hourioux C,Blanchard E,Brand D,Ait-Goughoulte M

    更新日期:2004-03-01 00:00:00

  • The mitogen-inducible gene-6 is involved in regulation of cellular senescence in normal diploid fibroblasts.

    abstract:BACKGROUND INFORMATION:The mitogen-inducible gene-6 (Mig-6) is a non-kinase scaffolding adaptor protein. It has been shown that Mig-6 may play important roles in regulating stress response, maintaining homeostasis and functioning as a tumour suppressor. In this study, we investigated the role of Mig-6 in cellular senes...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201200052

    authors: Xie B,Zhao L,Chen H,Jin B,Mao Z,Yao Z

    更新日期:2013-10-01 00:00:00

  • The potential role of caveolin-1 in inhibition of aquaporins during the AVD.

    abstract:BACKGROUND INFORMATION:During apoptosis, the first morphological change is a distinct cell shrinkage known as the AVD (apoptotic volume decrease). This event is driven by a loss of intracellular K(+), which creates an osmotic gradient, drawing water out of the cell through AQPs (aquaporins). Loss of water in balance wi...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20040131

    authors: Jablonski EM,Hughes FM Jr

    更新日期:2006-01-01 00:00:00

  • Fission yeast cytoskeletons and cell polarity factors: connecting at the cortex.

    abstract::Cell polarity is a fundamental property of cells from unicellular to multicellular organisms. Most of the time, it is essential so that the cells can achieve their function. The fission yeast Schizosaccharomyces pombe is a powerful genetic model organism for studying the molecular mechanisms of the cell polarity proce...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1042/BC20060048

    authors: La Carbona S,Le Goff C,Le Goff X

    更新日期:2006-11-01 00:00:00

  • Length of the selectivity filter of aquaporin-1.

    abstract::We have characterized the selectivity filter of the water channel aquaporin-1 (AQP1) of proximal straight tubules (PST), as an equivalent cylindrical structure with a diameter of approximately 4.5 A, where water molecules single file. We report here efforts to evaluate its length. PST were dissected from rabbit kidney...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Whittembury G,González E,Gutiérrez AM,Echevarría M,Hernández CS

    更新日期:1997-08-01 00:00:00

  • Presence of aquaporin and V-ATPase on the contractile vacuole of Amoeba proteus.

    abstract:BACKGROUND INFORMATION:The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90]. RESULTS:In the present study, we cloned an AQP gene from A. proteus [A...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20070091

    authors: Nishihara E,Yokota E,Tazaki A,Orii H,Katsuhara M,Kataoka K,Igarashi H,Moriyama Y,Shimmen T,Sonobe S

    更新日期:2008-03-01 00:00:00

  • Characterization of cell types in human breast tumor primary cultures.

    abstract::Primary cultures of cells from breast carcinomas were attempted in 74 cases. Growth was observed in 46 cases. Using immunochemical demonstration of keratin proteins (KER), epithelial membrane antigen (EMA) and carcinoembryonic antigen (CEA), three morphologically distinct cell populations were characterized and descri...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1987.tb00574.x

    authors: Muller D,Fricker JP,Millon-Collard R,Abecassis J,Pusel J,Eber M,Methlin G

    更新日期:1987-01-01 00:00:00

  • Cell death, non-invasively assessed by intrinsic fluorescence intensity of NADH, is a predictive indicator of functional differentiation of embryonic stem cells.

    abstract:BACKGROUND INFORMATION:Continued advances in stem cell biology and stem cell transplantation rely on non-invasive biomarkers to characterise cells and stem cell aggregates. The non-invasive quality of such biomarkers is essential because exogenous labels, probes or reporters can unintentionally and dramatically alter s...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201100091

    authors: Buschke DG,Squirrell JM,Fong JJ,Eliceiri KW,Ogle BM

    更新日期:2012-06-01 00:00:00

  • Efficient in vitro myogenic reprogramming of human primary mesenchymal stem cells and endothelial cells by Myf5.

    abstract:BACKGROUND INFORMATION:The identification of a source of stem cells able to regenerate skeletal muscle was the goal of numerous studies with the aim to develop new therapeutic approaches for genetic muscle diseases or muscle injuries. A series of studies have demonstrated that stem cells derived from various tissues ma...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20100112

    authors: Dimicoli-Salazar S,Bulle F,Yacia A,Massé JM,Fichelson S,Vigon I

    更新日期:2011-11-01 00:00:00

  • Bacterial lipopolysaccharide modulates protein kinase C signalling in Lymnaea stagnalis haemocytes.

    abstract::Our knowledge of cell signalling pathways in the molluscan immune system and their response to immunological challenge is currently poor. The present study focused on the Protein Kinase C (PKC) pathway in the immune cells (haemocytes) of Lymnaea stagnalis and its response following exposure to bacterial lipopolysaccha...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/j.biolcel.2003.09.001

    authors: Walker AJ,Plows LD

    更新日期:2003-11-01 00:00:00

  • Acid secretion and membrane reorganization in single gastric parietal cell in primary culture.

    abstract::A digitally-enhanced videomicroscopy study of rabbit gastric parietal cells in primary culture was performed using alternate observations with differential interference contrast and fluorescence optics of cells mounted and perfused on a temperature-controlled microscope stage. The effect of histamine, a physiological ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(90)90349-8

    authors: Mangeat P,Gusdinar T,Sahuquet A,Hanzel DK,Forte JG,Magous R

    更新日期:1990-01-01 00:00:00

  • Neutral aminopeptidase: a potential marker enzyme of the amphibian urinary bladder epithelial cell apical membrane.

    abstract::Antidiuretic hormone induces, in the apical plasma membrane of amphibian urinary bladder epithelial cells, the exocytotic insertion of intramembranous particle aggregates that probably contain water channels. Purification of the apical membrane is a way to characterize the aggregates. The isolation of such purified me...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Tacnet F,Chevalier J,Coudrier E,Berthonaud V,Ripoche P

    更新日期:1989-01-01 00:00:00

  • NFAT directly regulates Nkx2-5 transcription during cardiac cell differentiation.

    abstract:BACKGROUND INFORMATION:The transcription factor NFAT (nuclear factor of activated T-cell) family comprises important regulators in immuno-responses and mouse embryonic development, including early cardiovascular and heart valve development. The mechanism involved, however, is not fully understood. Nkx2-5 (NK2 transcrip...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20080108

    authors: Chen Y,Cao X

    更新日期:2009-06-01 00:00:00

  • Comparative aspects of circulating erythrocytes in the trophic and reproductive phases of European eel. Ultrastructure and cytochemistry.

    abstract::Different cytochemical and morphological parameters were used to compare the functional patterns of the erythrocytes in the transition from the trophic (yellow eel) to the reproductive (silver eel) phases of European eel (Anguilla anguilla L.). Data on the nucleus were obtained by microdensitometric (Feulgen reaction)...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1985.tb00402.x

    authors: Barni S,Gerzeli G

    更新日期:1985-01-01 00:00:00

  • Structural determinants involved in the activation and regulation of G protein-coupled receptors: lessons from the alpha1-adrenegic receptor subtypes.

    abstract::The aim of a large number of studies on G protein-coupled receptors was centered on understanding the structural basis of their main functional properties. Here, we will briefly review the results obtained on the alpha1-adrenergic receptor subtypes belonging to the rhodopsin-like family of receptors. These findings co...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.biolcel.2004.03.009

    authors: Cotecchia S,Stanasila L,Diviani D,Björklöf K,Rossier O,Fanelli F

    更新日期:2004-06-01 00:00:00

  • The integrative role of cryo electron microscopy in molecular and cellular structural biology.

    abstract::After gradually moving away from preparation methods prone to artefacts such as plastic embedding and negative staining for cell sections and single particles, the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of biological objects of various siz...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1111/boc.201600042

    authors: Orlov I,Myasnikov AG,Andronov L,Natchiar SK,Khatter H,Beinsteiner B,Ménétret JF,Hazemann I,Mohideen K,Tazibt K,Tabaroni R,Kratzat H,Djabeur N,Bruxelles T,Raivoniaina F,Pompeo LD,Torchy M,Billas I,Urzhumtsev A,Klahol

    更新日期:2017-02-01 00:00:00