Abstract:
:A digitally-enhanced videomicroscopy study of rabbit gastric parietal cells in primary culture was performed using alternate observations with differential interference contrast and fluorescence optics of cells mounted and perfused on a temperature-controlled microscope stage. The effect of histamine, a physiological effector of acid secretion, was followed. Isolated parietal cells possess an internal apical vacuole, which kept the cell in a pseudopolarized state. This apical vacuole is a site of acid secretion. This was demonstrated by the direct visualization of the uptake of the fluorescent weak base 9-amino acridine and of the concomitant enormous swelling of the acid vacuole which reached an estimated size of 3-7 times the normal cell volume. This morphological change of shape and acidification of apical vacuoles was fully reversible and cells could respond to successive stimulations. A quantitative study of these events provided a value of the acid accumulation index for each single cell in response to histamine. Individual cell response varied within a factor of 7. The cellular localization of the proton pump complex responsible for acid secretion and of the major components of the secretory microvilli, actin and ezrin, a histamine-dependent phosphorylation target of protein kinase A, were detected by indirect immunofluorescence microscopy in resting and stimulated cells. Both actin and ezrin colocalized at the apical vacuole membrane in resting and stimulated cells, whereas the proton pump shifted from an intracytoplasmic pool to the apical vacuole membrane upon stimulation.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Mangeat P,Gusdinar T,Sahuquet A,Hanzel DK,Forte JG,Magous Rdoi
10.1016/0248-4900(90)90349-8subject
Has Abstractpub_date
1990-01-01 00:00:00pages
223-31issue
3eissn
0248-4900issn
1768-322Xjournal_volume
69pub_type
杂志文章abstract::This review is concerned with the formation during ciliogenesis of centrioles and basal bodies, primarily in epithelial multi-ciliated cells from the developing vertebrate respiratory and reproductive tracts. During ciliated cell differentiation, in these as well as in other cell types, cilium formation is preceded by...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/0248-4900(91)90075-x
更新日期:1991-01-01 00:00:00
abstract::We have characterized the selectivity filter of the water channel aquaporin-1 (AQP1) of proximal straight tubules (PST), as an equivalent cylindrical structure with a diameter of approximately 4.5 A, where water molecules single file. We report here efforts to evaluate its length. PST were dissected from rabbit kidney...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract:BACKGROUND INFORMATION:Homotypic internalisation of tumour cells has frequently been observed in tumour tissue sections. Events of non-professional phagocytosis, however, may also occur in normal tissue if the number of dying cells exceeds the phagocytic capacity of professional phagocytes such as macrophages and dendr...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201400090
更新日期:2015-10-01 00:00:00
abstract::We used a high-resolution STEM to attempt to recognize the spatial localization of the macromolecular components of ribosomes isolated from Tetrahymena pyriformis. Images obtained either by the annular dark field detector or by the ratio-contrast mode (Z-contrast) show a ribosome as constituted of rounded dark areas, ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00271.x
更新日期:1984-01-01 00:00:00
abstract::Flow DNA analysis was performed on samples from 71 surgically removed lung cancers and from 145 patients undergoing bronchoscopy. Abnormal DNA stem lines, characterized by their DNA index (DI), were frequently observed in operated lung carcinomas (87%). Two or three abnormal DNA stem lines were discovered simultaneous...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00494.x
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND INFORMATION:Netrin-1 is a bi-functional cue that attracts or repels different classes of neurons during development. The netrin-1 receptor DCC (deleted in colorectal cancer) acts as a tyrosine kinase-associated receptor to mediate the attractive response towards netrin-1. The lipid raft-localized Src family ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20070108
更新日期:2009-02-01 00:00:00
abstract::Inside-out porcine thyroid follicles in culture undergo polarity reversal after being embedded in collagen gel. The newly-formed follicles reexpress some specific thyroid functions lost in inside-out follicles (Chambard et al., 1984. We present here an ultrastructural study of the inversion of polarity in this model s...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00463.x
更新日期:1986-01-01 00:00:00
abstract::The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90343-2
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND INFORMATION:The rod outer segment (OS) is the specialised organelle where phototransduction takes place. Our previous proteomic and biochemical analyses on purified rod disks showed the functional expression of the respiratory chain complexes I-IV and F1 Fo -ATP synthase in OS disks, as well as active solubl...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201300003
更新日期:2013-08-01 00:00:00
abstract::Although much of eukaryotic cell biology is highly conserved, the most biomedically relevant organisms are often the most difficult or expensive to study. Non-vertebrate model organisms offer a practical and accessible alternative. Large collections of loss-of-function mutants have now been generated in the nematode C...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20070005
更新日期:2007-06-01 00:00:00
abstract::The major finding of the present study is that the ultrastructural organization of the neuromuscular synapse can be modified by a small, 4-week-long, physiological increase in the locomotor activity of the extensor digitorum longus muscle of normal adult rats trained to walk. This study measures these plastic adaptati...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(99)80078-1
更新日期:1997-03-01 00:00:00
abstract::The ciliated protozoan Paramecium harbours a particularly large diversity of microtubule networks, ranging from the elaborate and stable ciliary axonemes and basal bodies to very dynamic cytoplasmic, cortical or intranuclear arrays. Their organization and individual cycle of assembly/disassembly are well known and ext...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(97)89840-1
更新日期:1996-01-01 00:00:00
abstract::It is now well established that malignant transformation of eucaryotic cells is concomitant with typical alterations of glycosylation and the expression pattern of endogenous lectins. In parallel, oncogene transfection studies revealed a correlation between the expression of some of these genes, the transformed state ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1111/j.1768-322x.1993.tb00899.x
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND INFORMATION:Our previous studies have shown that calreticulin, a Ca2+-binding chaperone located in the endoplasmic reticulum, affects cell-substratum adhesions via the induction of vinculin and N-cadherin. Cells overexpressing calreticulin contain more vinculin than low expressers and make abundant contacts ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20060105
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND INFORMATION:CLEM (correlative live cell and electron microscopy) seeks to bridge the data acquired with different imaging strategies, typically between light microscopy and electron microscopy. It has been successfully applied in cell cultures, although its use in multicellular systems is hampered by difficu...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20090096
更新日期:2009-12-04 00:00:00
abstract::An interaction between human poly(A)-binding protein (PABP) et human eRF3 has been demonstrated using a double-hybrid approach and in vitro assays. Here, we show that the binding of both proteins is conserved through evolution. We also demonstrate that the last 39 C-terminal amino acids of PABP contain the interface t...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(02)01194-2
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND INFORMATION:The interferon (IFN)-inducible protein TRIM22 (Staf50) is a member of the tripartite motif protein family and has been suggested a role in the regulation of viral replication as well as of protein ubiquitylation. In addition, we have previously shown that TRIM22 is a direct target gene for the tu...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201100099
更新日期:2012-08-01 00:00:00
abstract::Cell polarity is a fundamental property of cells from unicellular to multicellular organisms. Most of the time, it is essential so that the cells can achieve their function. The fission yeast Schizosaccharomyces pombe is a powerful genetic model organism for studying the molecular mechanisms of the cell polarity proce...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20060048
更新日期:2006-11-01 00:00:00
abstract::Flow cytometry (FCM) analysis of cellular DNA content has become an increasingly important clinical research tool for the measurement and identification of abnormal cell populations. Examination of cellular DNA content can provide information for use in cell cycle analysis in cancer studies. Most researchers would agr...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/0248-4900(93)90116-v
更新日期:1993-01-01 00:00:00
abstract::Total mRNA extracted from the brain of a marine worm, Nereis diversicolor, were in vitro translated using 2 cell-free systems: rabbit reticulocyte lysate and wheat germ extract. Among numerous products newly translated in both systems, only one class of 70 kDa polypeptides immunoprecipitated when we used a mixture of ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(91)90054-q
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND INFORMATION:The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90]. RESULTS:In the present study, we cloned an AQP gene from A. proteus [A...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20070091
更新日期:2008-03-01 00:00:00
abstract:BACKGROUND INFORMATION:Caspase-dependent and -independent death mechanisms are involved in apoptosis in a variety of human carcinoma cells treated with antineoplastic compounds. Our laboratory has reported that p53 is a key contributor of mitochondrial apoptosis in cervical carcinoma cells after staurosporine exposure....
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20080164
更新日期:2009-06-04 00:00:00
abstract::Dynamic secondary ion mass spectrometry (SIMS) has been utilised to study the post-mortem distribution of aluminium in air-dried frozen sections from unfixed, unstained human brain in order to minimise contamination of the tissue and avoid redistribution and extraction of endogenous tissue aluminium. Substrates, sputt...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(92)90016-t
更新日期:1992-01-01 00:00:00
abstract::Genome replication and assembly of viruses often takes place in specific intracellular compartments where viral components concentrate, thereby increasing the efficiency of the processes. For a number of viruses the formation of 'factories' has been described, which consist of perinuclear or cytoplasmic foci that most...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20040058
更新日期:2005-02-01 00:00:00
abstract:BACKGROUND INFORMATION:There are several reports indicating that starved fibroblasts show higher proliferation rates when re-fed with foetal bovine serum. We have evidence demonstrating that this phenomenon is related to secretory proteins which may be beneficial to wound healing. RESULTS:After re-feeding, 16 and 72 h...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201300063
更新日期:2014-05-01 00:00:00
abstract::SaOS-2 cell line presents osteoblastic characteristics which can be modulated by specific agonists involving also phosphoinositide breakdown. In order to determine whether SaOS-2 cells display a phosphoinositide signalling system not only at the cytosol-cell membrane level but also, as recently reported for other cell...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(93)90143-3
更新日期:1993-01-01 00:00:00
abstract::The distribution of viral RNA molecules in HeLa cells infected with adenovirus type 5 (Ad5) was determined by in situ hybridization at the ultrastructural level at an intermediate stage of nuclear transformation, when viral DNA synthetic activities were maximal but progeny viruses were still sparse. Transcription site...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(91)90060-z
更新日期:1991-01-01 00:00:00
abstract::The influence of triiodothyronine (T3) on avian myoblast proliferation and differentiation was studied in secondary cultures using plating densities of 2,500 and 7,000 cells/cm2. Culture media were depleted of T3 (control myoblasts) and increasing amounts were then added to concentrations of 0.6, 3 and 15 nM T3 (treat...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(93)90129-3
更新日期:1993-01-01 00:00:00
abstract::This study was undertaken to evaluate the effects of caerulein, a CCK analog, on the different cell populations of the pancreatic tissue and their respective turnover. Rats received saline or caerulein subcutaneously and 3H-thymidine intraperitoneally three times a day for 4 days. They were sacrificed immediately afte...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::beta-Catenin was initially characterized as a protein interacting with the cadherin cytoplasmic tail and regulating cell-cell contacts and actin cytoskeleton interactions. Moreover, the gene coding for the Drosophila orthologue of beta-catenin, armadillo, was independently identified downstream of wingless in the segm...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20040531
更新日期:2005-12-01 00:00:00