Presence of aquaporin and V-ATPase on the contractile vacuole of Amoeba proteus.

Abstract:

BACKGROUND INFORMATION:The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90]. RESULTS:In the present study, we cloned an AQP gene from A. proteus [ApAQP (A. proteus AQP)] that encodes a 295-amino-acid protein. The protein has six putative TMs (transmembrane domains) and two NPA (Asn-Pro-Ala) motifs, which are conserved among various AQPs and are thought to be involved in the formation of water channels that span the lipid bilayer. Using Xenopus oocytes, we have demonstrated that the ApAQP protein product can function as a water channel. Immunofluorescence microscopy with anti-ApAQP antibody revealed that ApAQP is detected on the CV membrane and on the vesicles around the CV. The presence of V-ATPase (vacuolar H+-ATPase) on the vesicle membrane around the CV was also detected. CONCLUSIONS:Our data on ApAQP allow us to provide the first informed explanation of the high water permeability of the CV membrane in amoeba. Moreover, the results suggest that vesicles possessing V-ATPase are involved in generating an osmotic gradient. Based on our findings, we propose a new hypothesis for the mechanism of CV function.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Nishihara E,Yokota E,Tazaki A,Orii H,Katsuhara M,Kataoka K,Igarashi H,Moriyama Y,Shimmen T,Sonobe S

doi

10.1042/BC20070091

subject

Has Abstract

pub_date

2008-03-01 00:00:00

pages

179-88

issue

3

eissn

0248-4900

issn

1768-322X

pii

BC20070091

journal_volume

100

pub_type

杂志文章
  • Vasopressin-induced intramembrane particle aggregates. A dose-response relationship in the isolated cortical collecting duct of the rabbit kidney.

    abstract::The water permeability of collecting ducts is greatly increased by the antidiuretic hormone, vasopressin (VP). Freeze-fracture studies were carried out to test if this permeability increase is associated with the appearance of intramembrane particle (IMP) aggregates and whether increased doses of VP lead to an increas...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Kubat B,Lorenzen M,Reale E

    更新日期:1989-01-01 00:00:00

  • Recognition of xenogeneic erythrocytes: the GalNAc/Gal-particle receptor of rat liver macrophages mediates or participates in recognition.

    abstract::We studied mechanisms that mediate recognition of human erythrocytes (HRBC) and sheep erythrocytes (SRBC) by rat liver macrophages. We used an in vitro cell binding assay that allows spontaneous formation of cell contacts. Binding of HRBC to rat macrophages shows the following characteristics: inhibition studies with ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1987.tb00558.x

    authors: Mohr M,Kolb H,Kolb-Bachofen V,Schlepper-Schäfer J

    更新日期:1987-01-01 00:00:00

  • Effects of retinoic acid metabolites on proliferation and differentiation of the clonal rhabdomyosarcoma cell line BA-HAN-1C.

    abstract::The clonal rat rhabdomyosarcoma cell line BA-HAN-1C is composed of proliferating mononuclear cells, some of which spontaneously fuse to terminally differentiated myotube-like giant cells. This cell line has been shown to be susceptible to differentiation induction with all-trans retinoic acid (RA). Since it is still u...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(94)90052-3

    authors: Ramp U,Gerharz CD,Eifler E,Biesalski HK,Gabbert HE

    更新日期:1994-01-01 00:00:00

  • Identification of novel adhesion proteins in mouse peritoneal macrophages.

    abstract::When mouse peritoneal macrophages were made to adhere firmly on glass surface and then removed by sequential treatment with hypotonic triethanolamine and Nonidet P-40, a set of proteins were found to be left behind at the sites of adherent cells. Such glass-adherent proteins were detected as round or ellipsoidal patch...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(92)90200-k

    authors: Tomita M,Ishikawa H

    更新日期:1992-01-01 00:00:00

  • Modulations of the epithelial phenotype and functional activity of cultured bovine tracheal gland cells: dependence on the culture medium and passage number.

    abstract::Bovine tracheal gland (BTG) cells in culture show an epithelial-fibroblastoid transition after several passages. To investigate these BTG cell phenotype changes, we studied the effects of both the culture medium and passage number on the expression of epithelial cytoskeletal proteins and glandular serous cell markers....

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(91)90008-b

    authors: Benali R,Dupuit F,Chevillard M,Jacquot J,Haye B,Puchelle E

    更新日期:1991-01-01 00:00:00

  • The expression of carbonic anhydrases II and IV in the human pancreatic cancer cell line (Capan 1) is associated with bicarbonate ion channels.

    abstract::Human pancreatic ductal cells of the Capan 1 cell line differentiate progressively during growth. After the exponential growth phase, the cells elongate and become polarized with their apical poles covered by microvilli and separated from the basolateral pole by tight junctions. In this stationary phase, they form dom...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/s0248-4900(94)80004-9

    authors: Mahieu I,Becq F,Wolfensberger T,Gola M,Carter N,Hollande E

    更新日期:1994-01-01 00:00:00

  • Polarity reversal of inside-out thyroid follicles cultured within collagen gel: an ultrastructural study.

    abstract::Inside-out porcine thyroid follicles in culture undergo polarity reversal after being embedded in collagen gel. The newly-formed follicles reexpress some specific thyroid functions lost in inside-out follicles (Chambard et al., 1984. We present here an ultrastructural study of the inversion of polarity in this model s...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1986.tb00463.x

    authors: Barriere H,Chambard M,Mauchamp J,Gabrion J

    更新日期:1986-01-01 00:00:00

  • Retinal pigment epithelial cells use a MerTK-dependent mechanism to limit the phagocytic particle binding activity of αvβ5 integrin.

    abstract:BACKGROUND INFORMATION:αvβ5 integrin and Mer tyrosine kinase (MerTK) receptors reside at the apical surface of the retinal pigment epithelium (RPE) in the eye to promote the diurnal, synchronised phagocytosis of shed photoreceptor outer segment fragments (POS) that is critical for vision. Phagocytosis assays studying R...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201100076

    authors: Nandrot EF,Silva KE,Scelfo C,Finnemann SC

    更新日期:2012-06-01 00:00:00

  • Neuropeptide Y expression, localization and cellular transducing effects in HUVEC.

    abstract:BACKGROUND INFORMATION:NPY (neuropeptide Y) may have an effect on the properties of vascular endothelial cells such as pro-angiogenic effects and potentiation of noradrenaline-induced vasoconstriction. In HUVEC (human umbilical-vein endothelial cells), immunoreactive neuropeptide Y has been detected, but NPY synthesis,...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20040102

    authors: Silva AP,Kaufmann JE,Vivancos C,Fakan S,Cavadas C,Shaw P,Brunner HR,Vischer U,Grouzmann E

    更新日期:2005-06-01 00:00:00

  • Redistribution of microvilli and membrane enzymes in isolated rat proximal tubule cells.

    abstract::Isolated cells from the proximal tubule have previously been used to study specific renal physiological processes. Here we analysed the structural changes induced by mechanical isolation on rat proximal tubule cells. As major modifications we detected: 1) a redistribution of brush border microvilli, which spread out o...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(92)90026-w

    authors: Rebelo L,Carmo-Fonseca M,Moura TF

    更新日期:1992-01-01 00:00:00

  • The imaging and quantification of aluminium in the human brain using dynamic secondary ion mass spectrometry (SIMS).

    abstract::Dynamic secondary ion mass spectrometry (SIMS) has been utilised to study the post-mortem distribution of aluminium in air-dried frozen sections from unfixed, unstained human brain in order to minimise contamination of the tissue and avoid redistribution and extraction of endogenous tissue aluminium. Substrates, sputt...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(92)90016-t

    authors: Candy JM,Oakley AE,Mountfort SA,Taylor GA,Morris CM,Bishop HE,Edwardson JA

    更新日期:1992-01-01 00:00:00

  • The lateral line of zebrafish: a model system for the analysis of morphogenesis and neural development in vertebrates.

    abstract::The lateral line of the zebrafish has many of the advantages that made the sensory organs of Drosophila a very productive model system: 1) it comprises a set of discrete sense organs (neuromasts) arranged in a defined, species-specific pattern, such that each organ can be individually recognized; 2) the neuromasts are...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.biolcel.2003.10.005

    authors: Dambly-Chaudière C,Sapède D,Soubiran F,Decorde K,Gompel N,Ghysen A

    更新日期:2003-12-01 00:00:00

  • Developmentally programmed DNA rearrangement in Tetrahymena thermophila: isolation and sequence characterization of three new alternative deletion systems.

    abstract::Extensive developmentally programmed DNA rearrangements, including thousands of internal deletions, occur in the differentiating somatic macronucleus in Tetrahymena thermophila. Some deletion systems involve the use of multiple alternative deletion sites. We report here the cloning and the sequences of three new alter...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(96)84773-3

    authors: Chau MF,Orias E

    更新日期:1996-01-01 00:00:00

  • Isolated cytoskeletons of human blood platelets: dark-field imaging of coiled and uncoiled microtubules.

    abstract::Detergent extraction of human blood platelets pre-treated with Taxol to stabilize microtubules allows isolation of marginal band (MB) cytoskeletons. We studied MB cytoskeleton structure using dark-field light microscopy and negative stain electron microscopy (EM). Dark-field illumination clearly demonstrated the "hoop...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(88)90002-0

    authors: Kowit JD,Linck RW,Kenney DM

    更新日期:1988-01-01 00:00:00

  • Immunocytochemical localization of arabinoxylans in the cell wall of maize apical internode after microbial degradation in the rumen.

    abstract::Arabinoxylans were localised by immunocytochemistry using polyclonal antibodies in the cell walls of the apical internode of maize after degradation in the rumen. In order to understand the significance of arabinoxylan in digestibility property, two lines of maize differing in digestibility were used. Wide variations ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/j.1768-322x.1996.tb00988.x

    authors: Migné C,Prensier G,Cornu A,Grenet E

    更新日期:1996-01-01 00:00:00

  • Changes in a nuclear matrix antigen during the cell cycle: interphase and mitotic cells.

    abstract::We studied the behaviour in interphase and mitotic human cells of a 125 kDa (pI 6.5) antigen, associated with the nuclear matrix and detected in proliferating cells. Indirect immunofluorescence with a specific monoclonal antibody reveals that during interphase in WISH and Namalwa cells, as well as phytohaemagglutinin-...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Todorov IT,Philipova PN,Zhelev NZ,Hadjiolov AA

    更新日期:1988-01-01 00:00:00

  • A hypothesis on p34cdc2 sequestration based on the existence of Ca(2+)-coordinated changes in H+ and MPF activities during Xenopus egg activation [corrected].

    abstract::The entry into, and exit from, mitosis are controlled by a universal M-phase promoting factor (MPF) composed of at least p34cdc2 and a cyclin. Embryonic systems are convenient for studying the association and dissociation of the active MPF complex because oocytes and eggs are naturally arrested at a specific point of ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/0248-4900(92)90137-p

    authors: Charbonneau M,Grandin N

    更新日期:1992-01-01 00:00:00

  • The costa of trichomonads: A complex macromolecular cytoskeleton structure made of uncommon proteins.

    abstract:BACKGROUND INFORMATION:The costa is a prominent striated fibre that is found in protozoa of the Trichomonadidae family that present an undulating membrane. It is composed primarily of proteins that have not yet been explored. In this study, we used cell fractionation to obtain a highly enriched costa fraction whose str...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201600050

    authors: de Andrade Rosa I,Caruso MB,de Oliveira Santos E,Gonzaga L,Zingali RB,de Vasconcelos ATR,de Souza W,Benchimol M

    更新日期:2017-06-01 00:00:00

  • 'Should I stay or should I go?': myosin V function in organelle trafficking.

    abstract::Actin- and microtubule-based motors can propel different cargos along filaments. Within cells, they control the distribution of membrane-bound compartments by performing complementary tasks. Organelles make long journeys along microtubules, with class V myosins ensuring their capture and their dispersal in actin-rich ...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1042/BC20070021

    authors: Desnos C,Huet S,Darchen F

    更新日期:2007-08-01 00:00:00

  • TPA induces apoptosis in MPC-11 mouse plasmacytoma cells grown in serum-free medium.

    abstract::Apoptotic-like events could be rapidly induced by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) in cells of the mouse plasmacytoma cell line MPC-11 grown in serum-free medium. Indicators for apoptosis were morphological changes visualized by light and electron microscopy, such as chromatin condensation ...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Giese G,Schmidt J,Gilbert M,Albrecht R,Traub P

    更新日期:1997-05-01 00:00:00

  • Inherited retinal degenerations: therapeutic prospects.

    abstract::Retinitis pigmentosa (RP) is a heterogeneous group of inherited retinal degenerative diseases, characterized by the progressive death of rod and cone photoreceptors. A tremendous genetic heterogeneity is associated with the RP phenotype. Most mutations affect rods selectively and, through an unknown pathway, cause the...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.biolcel.2004.01.006

    authors: Delyfer MN,Léveillard T,Mohand-Saïd S,Hicks D,Picaud S,Sahel JA

    更新日期:2004-05-01 00:00:00

  • Regulation of with-no-lysine kinase signaling by Kelch-like proteins.

    abstract::In 2001, with-no-lysine (WNK) kinases were identified as the genes responsible for the human hereditary hypertensive disease pseudohypoaldosteronism type II (PHAII). It took a further 6 years to clarify that WNK kinases participate in a signaling cascade with oxidative stress-responsive gene 1 (OSR1), Ste20-related pr...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1111/boc.201300069

    authors: Uchida S,Sohara E,Rai T,Sasaki S

    更新日期:2014-02-01 00:00:00

  • Control of CREB phosphorylation and its role for induction of melatonin synthesis in rat pinealocytes.

    abstract::Phosphorylation of the transcription factor CREB appears as an important step in the signal transduction cascade that activates melatonin biosynthesis in the mammalian pineal organ. We have studied the mechanisms causing CREB phosphorylation by immunocytochemical and immunochemical demonstration of phosphorylated CREB...

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:10.1016/s0248-4900(98)80006-3

    authors: Maronde E,Schomerus C,Stehle JH,Korf HW

    更新日期:1997-11-01 00:00:00

  • Characterisation of endogenous Claudin-1 expression, motility and susceptibility to hepatitis C virus in CRISPR knock-in cells.

    abstract:BACKGROUND INFORMATION:Claudin-1 (CLDN1) is a four-span transmembrane protein localised at cell-cell tight junctions (TJs), playing an important role in epithelial impermeability and tissue homoeostasis under physiological conditions. Moreover, CLDN1 expression is up-regulated in several cancers, and the level of CLDN1...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1111/boc.201900085

    authors: Clément CMH,Deffieu MS,Dorobantu CM,Baumert TF,Ayala-Nunez NV,Mély Y,Ronde P,Gaudin R

    更新日期:2020-05-01 00:00:00

  • Keratinization of rat vaginal epithelium. III. Effect of estradiol on keratinization.

    abstract::Effect of estradiol-17 beta on rat vaginal epithelial cells (VEC) was studied by transmission electron microscopy. During the normal estrous cycle in adult rats keratinization and exfoliation of VEC was observed only in the estrus phase, though tonofilament bundles were seen distributed in the cytoplasm of the VEC dur...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Gupta PD,Vijayasaradhi S,Reddy AG

    更新日期:1989-01-01 00:00:00

  • EYA1 mutations associated with the branchio-oto-renal syndrome result in defective otic development in Xenopus laevis.

    abstract:BACKGROUND INFORMATION:The BOR (branchio-oto-renal) syndrome is a dominant disorder most commonly caused by mutations in the EYA1 (Eyes Absent 1) gene. Symptoms commonly include deafness and renal anomalies. RESULTS:We have used the embryos of the frog Xenopus laevis as an animal model for early ear development to exa...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1042/BC20090098

    authors: Li Y,Manaligod JM,Weeks DL

    更新日期:2010-02-17 00:00:00

  • Immunodetection of lipopolysaccharide in macrophages during the processing of non invasive Shigella dysenteriae.

    abstract::The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/0248-4900(90)90343-2

    authors: Dacosta B,Ryter A,Mounier J,Sansonetti P

    更新日期:1990-01-01 00:00:00

  • Microspectrofluorometry of autofluorescence emission from human leukemic living cells under oxidative stress.

    abstract::Image cytometry was applied to study the intracellular localization of autofluorescence and the influence of an oxidative stress on this emission. K562 erythroleukemia cancer cells were analyzed with a microspectrofluorometer, coupled with a Argon laser (Ar+) (363 nm). From each cell, 15 x 15 emission spectra were rec...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:10.1016/s0248-4900(01)01135-2

    authors: Bondza-Kibangou P,Millot C,Dufer J,Millot JM

    更新日期:2001-11-01 00:00:00

  • Oligonucleotide biological activity: relationship to the cell cycle and nuclear transport.

    abstract::Previous studies suggest that oligodeoxynucleotide (ODN) cellular uptake is cell cycle-dependent which may have important implications in cancer cell targeting. To further our understanding of ODN transport and activity, this study examines the relationships between the cell cycle, ODN cellular uptake, intracellular t...

    journal_title:Biology of the cell

    pub_type: 杂志文章

    doi:

    authors: Wu-Pong S,Bard J,Huffman J,Jimerson J

    更新日期:1997-07-01 00:00:00

  • Centrosome structure and microtubule nucleation in animal cells.

    abstract::Genetic studies in the budding yeast have led to the molecular characterization of gamma-tubulin associated proteins and to the identification of orthologues in animal cells. While the gamma-tubulin complex is more complex in animal cells than in budding yeast, its function is probably maintained throughout evolution....

    journal_title:Biology of the cell

    pub_type: 杂志文章,评审

    doi:

    authors: Tassin AM,Bornens M

    更新日期:1999-05-01 00:00:00