Abstract：
BACKGROUND INFORMATION:There are several reports indicating that starved fibroblasts show higher proliferation rates when re-fed with foetal bovine serum. We have evidence demonstrating that this phenomenon is related to secretory proteins which may be beneficial to wound healing. RESULTS:After re-feeding, 16 and 72 h serum-starved fibroblasts showed the highest and lowest proliferation rates, 1.59 and 0.51-fold difference compared to the non-starved control, respectively (P < 0.05). However, the latest value could be normalised by incubating cells with 16 h-starved fibroblast cell culture supernatant (16-SFS), prior to re-feeding. A strong correlation was found between total protein level in starved fibroblast culture supernatants and post re-feeding proliferation rates (r(2) = 0.90, P < 0.001). Two-dimensional gel electrophoresis analysis of 16-SFS confirmed the presence of proteins with relative molecular weights of 10-120 kDa and pI ranging from 4 to 6. A significant difference in calcium influx course was found between 16-SFS and the negative control (Dulbecco's Modified Eagle Medium) (P < 0.05). There was no significant difference in Ca(2+) concentrations after 1 h between non-starved controls and 16-SFS-treated fibroblasts. The scratch test demonstrated that the 16-SFS is able to induce fibroblast migration. CONCLUSIONS:We concluded that human starved fibroblasts secrete proteins that are able to induce post re-feeding cell proliferation and fibroblasts migration, probably through the induction of a sustained calcium influx. This is worth being considered as a potential tool for wound healing.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Golpour M,Fattahi S,Niaki HA,Hadipoor A,Abedian Z,Ahangarian GR,Parsian H,Mosapour A,Khorasani HR,Vaziri HR,Bijani A,Mostafazadeh Adoi
10.1111/boc.201300063subject
Has Abstractpub_date
2014-05-01 00:00:00pages
139-50issue
5eissn
0248-4900issn
1768-322Xjournal_volume
106pub_type
杂志文章abstract：:Alpha 2-macroglobulin (alpha 2M) is a plasma inhibitor of proteinases, the steric mechanism of which is based on a considerable conformational change. The typical and distinct H-like shape of alpha 2M-chymotrypsin (alpha 2M-chy) complexes seen by electron microscopy led us to an ultrastructural study of the binding of...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(88)90092-5
更新日期：1988-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(00)01065-0
更新日期：2000-07-01 00:00:00
abstract：BACKGROUND INFORMATION:Loss of sensitivity to TGF-beta1 (transforming growth factor beta1)-induced growth arrest is an important step towards malignant transformation in human epithelial cells, and Id-1 (inhibitor of differentiation or DNA binding-1) has been associated with cell proliferation and cell-cycle progressio...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20060026
更新日期：2006-09-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00218.x
更新日期：1983-01-01 00:00:00
abstract：BACKGROUND INFORMATION:The assembly and maintenance of cilia depend on IFT (intraflagellar transport) mediated by molecular motors and their interplay with IFT proteins. Here, we have analysed the involvement of IFT proteins in the ciliogenesis of mammalian photoreceptor cilia. RESULTS:Electron microscopy revealed tha...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20110034
更新日期：2011-10-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20080164
更新日期：2009-06-04 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90369-e
更新日期：1990-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00271.x
更新日期：1984-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/bc20060130
更新日期：2007-10-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(96)81303-7
更新日期：1995-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(91)90069-y
更新日期：1991-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 传,历史文章,杂志文章
doi：10.1042/BC20050036
更新日期：2006-08-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90441-3
更新日期：1992-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90133-l
更新日期：1992-01-01 00:00:00
abstract：:Phosphorylation of the transcription factor CREB appears as an important step in the signal transduction cascade that activates melatonin biosynthesis in the mammalian pineal organ. We have studied the mechanisms causing CREB phosphorylation by immunocytochemical and immunochemical demonstration of phosphorylated CREB...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/s0248-4900(98)80006-3
更新日期：1997-11-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1990.tb00007.x
更新日期：1990-01-01 00:00:00
abstract：:The interaction of cells with extracellular matrix components contributes to their specific differentiation. We studied hepatic peroxisomes and their changing features during embryonic development, and we immunolocalized in the same tissue the extracellular matrix components laminin and collagen IV as well as the inte...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:Effect of estradiol-17 beta on rat vaginal epithelial cells (VEC) was studied by transmission electron microscopy. During the normal estrous cycle in adult rats keratinization and exfoliation of VEC was observed only in the estrus phase, though tonofilament bundles were seen distributed in the cytoplasm of the VEC dur...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/j.1768-322x.1994.tb00932.x
更新日期：1994-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00266.x
更新日期：1984-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20040531
更新日期：2005-12-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/0248-4900(91)90075-x
更新日期：1991-01-01 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20090060
更新日期：2009-09-14 00:00:00
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journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1984.tb00222.x
更新日期：1983-01-01 00:00:00
abstract：:cdc25 proteins are universally involved in the control of cell division. Using an original method of sequence analysis, cdc25 proteins from different sources were compared to protein phosphatases. Protein phosphatases could clearly be characterized as two distinct protein families, the phospho-seryl/threonyl phosphata...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(92)90134-m
更新日期：1992-01-01 00:00:00
abstract：:Bovine tracheal gland (BTG) cells in culture show an epithelial-fibroblastoid transition after several passages. To investigate these BTG cell phenotype changes, we studied the effects of both the culture medium and passage number on the expression of epithelial cytoskeletal proteins and glandular serous cell markers....
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(91)90008-b
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND INFORMATION:PCD (programmed cell death) is a common mechanism to remove unwanted and excessive cells from organisms. In several exocrine cell types, PCD mode of release of secretory products has been reported. The molecular mechanism of the release, however, is largely unknown. Our aim was to study the molec...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20070168
更新日期：2009-02-01 00:00:00
abstract：BACKGROUND INFORMATION:The identification of a source of stem cells able to regenerate skeletal muscle was the goal of numerous studies with the aim to develop new therapeutic approaches for genetic muscle diseases or muscle injuries. A series of studies have demonstrated that stem cells derived from various tissues ma...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20100112
更新日期：2011-11-01 00:00:00
abstract：BACKGROUND INFORMATION:Death receptors (DRs) induce intracellular signalling upon engagement of their cognate ligands, leading to apoptosis, cell survival or pro-inflammatory responses. In mammals, DR signalling is mediated by the recruitment of several DD (death domain)-containing molecules, such as FADD (Fas-associat...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20050091
更新日期：2006-08-01 00:00:00
