Abstract:
:Using gold labelled neoglycoproteins containing either alpha-D-glucose, N-acetyl-beta-D-glucosamine, alpha-D-mannose, 6-phospho-alpha-D-mannose, and alpha-L-fucose (BSA), we investigated their intranuclear binding sites in the TG human cell line. Although gold-labelled BSA did not give any noticeable labelling, the presence of 1% free BSA in the medium containing the gold labelled neoglycoproteins was revealed to be a key factor of the labelling. During interphase in the presence of free BSA most of the labelling was detected in the nucleoplasm. The border of the condensed chromatin, known to be the site of hnRNA synthesis as well as the interchromatin areas enriched in RNPs were labelled. Condensed chromatin also contained binding-sites. The nucleolus was seen to present low labelling in comparison with the labelling observed over the nucleoplasm. These nucleolar binding sites were located both in the dense fibrillar and granular components. No labelling could be detected over the fibrillar centers which are very conspicuous in this cell line. During mitosis sugar-binding sites were observed over the chromosomes. Data reported here show for the first time that lectin-like proteins and chromatin components are colocalized both during interphase and mitosis. In addition, within the nucleolus the presence of sugar-binding proteins was seen to be restricted to the dense fibrillar and granular components.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Bourgeois CA,Monsigny Mdoi
10.1111/j.1768-322x.1990.tb00007.xsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
119-26issue
2eissn
0248-4900issn
1768-322Xjournal_volume
69pub_type
杂志文章abstract::Actin- and microtubule-based motors can propel different cargos along filaments. Within cells, they control the distribution of membrane-bound compartments by performing complementary tasks. Organelles make long journeys along microtubules, with class V myosins ensuring their capture and their dispersal in actin-rich ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20070021
更新日期:2007-08-01 00:00:00
abstract::Vertebrate embryos are able to reconstitute the body plan when early blastomeres are deleted, but it is not known whether this is accomplished by cells local to the lesion or by a readjustment of the entire pattern of the embryo. We distinguished between these two possibilities by studying which embryonic cells change...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/j.biolcel.2004.06.002
更新日期:2004-09-01 00:00:00
abstract::The communication of calcium signals between cells is known to be operative between neurons where these signals integrate intimately with electrical and chemical signal communication at synapses. Recently, it has become clear that glial cells also exchange calcium signals between each other in cultures and in brain sl...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/j.biolcel.2003.10.007
更新日期:2004-02-01 00:00:00
abstract::Much remains to be learned about how cancer cells acquire the property of migration, a prerequisite for invasiveness and metastasis. Loss of p53 functions is assumed to be a crucial step in the development of many types of cancers, leading to dysregulation of cell cycle checkpoint controls and apoptosis. However, emer...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20050058
更新日期:2006-03-01 00:00:00
abstract::LDs (lipid droplets) have long been considered as inert particles used by the cells to store fatty acids and sterols as esterified non-toxic lipid species (i.e. triacylglycerols and steryl esters). However, accumulating evidence suggests that LDs behave as a dynamic compartment, which is involved in the regulation of ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20100144
更新日期:2011-06-01 00:00:00
abstract::During wound-healing in cultured frog skin fragments, fibronectin (FN) was detected in the dermal-epidermal junction. Intracellular fibronectin was stained using permeabilization and DAB immunoperoxidase. With electron microscopy intracytoplasmic FN granules were localized in the epidermal processes of the stratum ger...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1989.tb03013.x
更新日期:1989-01-01 00:00:00
abstract::The PAS-positive-calcium-sensitive (Ca-s) cells of the pars intermedia (PI) were studied in goldfish kept in fresh water (FW), deionized water (DW), 1/3 sea water (SW) and 1/3 Ca-free SW. Ultrastructural studies show that Ca-s cells of control goldfish kept in FW have a low activity with elongated or deeply indented n...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00202.x
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND INFORMATION:During apoptosis, the first morphological change is a distinct cell shrinkage known as the AVD (apoptotic volume decrease). This event is driven by a loss of intracellular K(+), which creates an osmotic gradient, drawing water out of the cell through AQPs (aquaporins). Loss of water in balance wi...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20040131
更新日期:2006-01-01 00:00:00
abstract::The major finding of the present study is that the ultrastructural organization of the neuromuscular synapse can be modified by a small, 4-week-long, physiological increase in the locomotor activity of the extensor digitorum longus muscle of normal adult rats trained to walk. This study measures these plastic adaptati...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(99)80078-1
更新日期:1997-03-01 00:00:00
abstract:BACKGROUND INFORMATION:Meningothelial cells (MECs) are the cellular components of the meninges protecting the brain and as such provide important barrier function for the central nervous system building the interface between neuronal tissue and the cerebrospinal fluid (CSF). MECs were previously shown to be involved in...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201300013
更新日期:2013-07-01 00:00:00
abstract::The microtubule-associated proteins MAPs 1 and 2 from pig brain have been found to react with antibodies directed against human ankyrin and spectrin, respectively (Bennett and Davis, 1981; Davis and Bennett, 1982). In a complementary approach we have prepared antibodies against MAP1 alpha. MAP1 gamma and MAP2 purified...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1985.tb00361.x
更新日期:1985-01-01 00:00:00
abstract::Image analysis was used to study the repair process of a circular mechanical lesion of confluent human endothelial cells in culture after irradiation (10 Gy) prior to wounding. Coverage of denuded areas 48 and 96 h after injury of endothelial cells was identical in control and irradiated cultures, although the labelin...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90313-r
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND INFORMATION:The identification of a source of stem cells able to regenerate skeletal muscle was the goal of numerous studies with the aim to develop new therapeutic approaches for genetic muscle diseases or muscle injuries. A series of studies have demonstrated that stem cells derived from various tissues ma...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20100112
更新日期:2011-11-01 00:00:00
abstract::The centrosome of the slime mould Dictyostelium discoideum displays a morphology markedly different from centriolar centrosomes or yeast spindle pole bodies, while fulfilling the same conserved functions in the organization of the microtubule cytoskeleton. Recent advances suggest that the Dictyostelium centrosome may ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(99)80092-6
更新日期:1999-05-01 00:00:00
abstract::Flow DNA analysis was performed on samples from 71 surgically removed lung cancers and from 145 patients undergoing bronchoscopy. Abnormal DNA stem lines, characterized by their DNA index (DI), were frequently observed in operated lung carcinomas (87%). Two or three abnormal DNA stem lines were discovered simultaneous...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00494.x
更新日期:1986-01-01 00:00:00
abstract::We studied the behaviour in interphase and mitotic human cells of a 125 kDa (pI 6.5) antigen, associated with the nuclear matrix and detected in proliferating cells. Indirect immunofluorescence with a specific monoclonal antibody reveals that during interphase in WISH and Namalwa cells, as well as phytohaemagglutinin-...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::Bovine tracheal gland (BTG) cells in culture show an epithelial-fibroblastoid transition after several passages. To investigate these BTG cell phenotype changes, we studied the effects of both the culture medium and passage number on the expression of epithelial cytoskeletal proteins and glandular serous cell markers....
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(91)90008-b
更新日期:1991-01-01 00:00:00
abstract::Extensive developmentally programmed DNA rearrangements, including thousands of internal deletions, occur in the differentiating somatic macronucleus in Tetrahymena thermophila. Some deletion systems involve the use of multiple alternative deletion sites. We report here the cloning and the sequences of three new alter...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(96)84773-3
更新日期:1996-01-01 00:00:00
abstract::Evidence is presented that two circumferential zones of cell envelope differentiation, the periseptal annuli, exist in E. coli as previously observed in S. typhimurium. The periseptal annulus is located at the division site of cells. A strain overproducing a periplasmic protein, PhoS (phosphate-binding protein) has be...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00274.x
更新日期:1984-01-01 00:00:00
abstract::Variant surface glycoprotein (VSG) of Trypanosoma brucei brucei AnTat 1.1 was released by means of the procedure described by Baltz et al. ([1976], Ann. Immunol. [Inst. Pasteur] 127C, 761-774). The concanavalin-A chromatography yielded 3 VSG fractions according to the addition, in the elution buffer, of alpha-methyl-D...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(88)90072-x
更新日期:1988-01-01 00:00:00
abstract::Oocyte maturation and early development have been intensively studied for well over 100 years. The earliest theory proposed that after fertilisation and during cell division determinants were unequally distributed to control cell fate; experimental proof came from using frog eggs (Roux, 1888). After understanding the ...
journal_title:Biology of the cell
pub_type: 传,社论,历史文章
doi:10.1016/j.biolcel.2004.01.002
更新日期:2004-04-01 00:00:00
abstract::Circulating glucocorticoid (GC) levels are thought to modulate the basal activity of pro-opiomelanocortin (POMC) neurons within the mediobasal hypothalamus (MBH) of the male rat. In a recent study we demonstrated that Fos-immunoreactivity (Fos-IR) was spontaneously induced throughout the dark phase of the light/dark c...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract:BACKGROUND INFORMATION:The costa is a prominent striated fibre that is found in protozoa of the Trichomonadidae family that present an undulating membrane. It is composed primarily of proteins that have not yet been explored. In this study, we used cell fractionation to obtain a highly enriched costa fraction whose str...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201600050
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND INFORMATION:The role of the LIM-domain-containing protein Ajuba was initially described in cell adhesion and migration processes and recently in mitosis as an activator of the Aurora A kinase. RESULTS:In the present study, we show that Ajuba localizes to centrosomes and kinetochores during mitosis. This loc...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20080060
更新日期:2009-04-01 00:00:00
abstract:BACKGROUND INFORMATION:The endometrial epithelial cell membrane is a key interface in female reproductive biology. Steroid hormones play a predominant role in cyclic changes which occur at this interface during the female menstrual cycle. Specific changes in the morphology of the endometrial epithelial cell surface bec...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20080189
更新日期:2009-06-04 00:00:00
abstract:BACKGROUND INFORMATION:Caspase-dependent and -independent death mechanisms are involved in apoptosis in a variety of human carcinoma cells treated with antineoplastic compounds. Our laboratory has reported that p53 is a key contributor of mitochondrial apoptosis in cervical carcinoma cells after staurosporine exposure....
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20080164
更新日期:2009-06-04 00:00:00
abstract::A general feature of the cell adhesion molecules belonging to the immunoglobulin family (Ig-CAMs) is to display a modular structure that provides a framework for multiple binding sites for other recognition molecules. Among this family, F3/contactin is a glycan phosphatidyl-inositol (GPI)-anchored molecule expressed b...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(02)00006-0
更新日期:2002-10-01 00:00:00
abstract::Porcine lutropin (pLH) and human choriogonadotropin (hCG) recognize the same hormonal receptor and elicit the same steroidogenic response in porcine Leydig cells in primary culture. We compared the variation in the number of occupied and free receptors present on the cell surface under short-term stimulation by the tw...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1987.tb00523.x
更新日期:1987-01-01 00:00:00
abstract::The micromeres, the first cells to be specified in sea urchin embryos, are generated by unequal cleavage at the fourth cell division. The micromeres differentiate autonomously to form spicules and dispatch signals to induce endomesoderm in the neighbouring macromeres cells in the embryo. Using a calcium indicator Fura...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/j.biolcel.2003.11.009
更新日期:2004-03-01 00:00:00
abstract::I am pleased to contribute to this special issue of Biology of the Cell in honor of Yoshio Masui. Oocyte maturation remains a small enough and young enough field that the authors assembled for this issue can trace the entire development of the field over the last 30 years, beginning with the early demonstration by Mas...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:
更新日期:1998-10-01 00:00:00