Abstract:
:Membrane fluidity of erythrocytes obtained from 15 children with trisomy 21 and 20 healthy controls were studied by measuring steady-state fluorescence anisotropy and fluorescence lifetime of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) incorporated in hemoglobin-free erythrocyte membranes. Our results demonstrate a significant decrease in DPH fluorescence anisotropy and a significant increase in TMA-DPH fluorescence anistropy in erythrocytes from subjects with trisomy 21. No significant differences between the two groups were observed in the fluorescence lifetime of DPH and TMA-DPH. These data suggest an increase in membrane fluidity in the interior part of the membrane and a decrease in fluidity at the lipid-water interface region. This could be in part attributed to an increased oxidative damage in trisomy 21.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Kantar A,Giorgi PL,Curatola G,Fiorini Rdoi
10.1016/0248-4900(92)90133-lsubject
Has Abstractpub_date
1992-01-01 00:00:00pages
135-8issue
2eissn
0248-4900issn
1768-322Xjournal_volume
75pub_type
杂志文章abstract:BACKGROUND INFORMATION:Continued advances in stem cell biology and stem cell transplantation rely on non-invasive biomarkers to characterise cells and stem cell aggregates. The non-invasive quality of such biomarkers is essential because exogenous labels, probes or reporters can unintentionally and dramatically alter s...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201100091
更新日期:2012-06-01 00:00:00
abstract::The microtubule-associated proteins MAPs 1 and 2 from pig brain have been found to react with antibodies directed against human ankyrin and spectrin, respectively (Bennett and Davis, 1981; Davis and Bennett, 1982). In a complementary approach we have prepared antibodies against MAP1 alpha. MAP1 gamma and MAP2 purified...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1985.tb00361.x
更新日期:1985-01-01 00:00:00
abstract::Retinitis pigmentosa (RP) is a heterogeneous group of inherited retinal degenerative diseases, characterized by the progressive death of rod and cone photoreceptors. A tremendous genetic heterogeneity is associated with the RP phenotype. Most mutations affect rods selectively and, through an unknown pathway, cause the...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/j.biolcel.2004.01.006
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND INFORMATION:Substantial evidence indicates the existence of NCSCs (neural crest-derived stem cells) in embryonic mandibular processes; however, they have not been fully investigated or isolated. The aim of the present study was to isolate stem cells from mandibular process during embryonic development by MAC...
journal_title:Biology of the cell
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doi:10.1042/BC20060012
更新日期:2006-10-01 00:00:00
abstract::Somatostatin-immunoreactive cells were localized on semithin and ultrathin sections of Epon-embedded samples of perch gastric mucosa, classically fixed with aldehydes and osmium tetroxide. On semithin sections, somatostatin cells were identified by using the immunoperoxidase method. The ultrastructural localization of...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1985.tb00375.x
更新日期:1985-01-01 00:00:00
abstract::Arabinoxylans were localised by immunocytochemistry using polyclonal antibodies in the cell walls of the apical internode of maize after degradation in the rumen. In order to understand the significance of arabinoxylan in digestibility property, two lines of maize differing in digestibility were used. Wide variations ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1996.tb00988.x
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND INFORMATION:In a previous study, we demonstrated that human neutrophils can develop membrane tubulovesicular extensions (TVEs) that are 160-250 nm in width and several micrometres long. These extensions, or cytonemes, are capable of establishing long-range contacts with other cells or bacteria. Cytonemes con...
journal_title:Biology of the cell
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更新日期:2015-05-01 00:00:00
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journal_title:Biology of the cell
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doi:10.1042/BC20040142
更新日期:2005-09-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(91)90008-b
更新日期:1991-01-01 00:00:00
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journal_title:Biology of the cell
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更新日期:2018-03-01 00:00:00
abstract::Genetic studies in the budding yeast have led to the molecular characterization of gamma-tubulin associated proteins and to the identification of orthologues in animal cells. While the gamma-tubulin complex is more complex in animal cells than in budding yeast, its function is probably maintained throughout evolution....
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:
更新日期:1999-05-01 00:00:00
abstract::After gradually moving away from preparation methods prone to artefacts such as plastic embedding and negative staining for cell sections and single particles, the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of biological objects of various siz...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
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更新日期:2017-02-01 00:00:00
abstract::The diversity of microtubular networks was analyzed in quail oviduct and in Paramecium cells using conventional and confocal immunofluorescence as well as pre- and post-embedding EM immunocytochemistry with a variety of anti-tubulin antibodies. The 6-11B-1 monoclonal antibody, specific for the post-translational acety...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(91)90069-y
更新日期:1991-01-01 00:00:00
abstract::We have characterized the selectivity filter of the water channel aquaporin-1 (AQP1) of proximal straight tubules (PST), as an equivalent cylindrical structure with a diameter of approximately 4.5 A, where water molecules single file. We report here efforts to evaluate its length. PST were dissected from rabbit kidney...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract:BACKGROUND INFORMATION:The assembly and maintenance of cilia depend on IFT (intraflagellar transport) mediated by molecular motors and their interplay with IFT proteins. Here, we have analysed the involvement of IFT proteins in the ciliogenesis of mammalian photoreceptor cilia. RESULTS:Electron microscopy revealed tha...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20110034
更新日期:2011-10-01 00:00:00
abstract::ERM (ezrin/radixin/moesin) proteins, concentrated in actin rich cell-surface structures, cross-link actin filaments with the plasma membrane. They are involved in the formation of microvilli, cell-cell adhesion, maintenance of cell shape, cell motility and membrane trafficking. Recent analyses reveal that they are not...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(00)01078-9
更新日期:2000-08-01 00:00:00
abstract:BACKGROUND INFORMATION:Cortactin contributes to growth cone morphogenesis by forming with dynamin, ring-shaped complexes that mechanically bundle and stabilise F-actin. However, the regulatory mechanism of cortactin action is poorly understood. RESULTS:Immunofluorescence microscopy revealed that protein kinase C (PKC)...
journal_title:Biology of the cell
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doi:10.1111/boc.201500032
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND INFORMATION:Our previous studies have shown that calreticulin, a Ca2+-binding chaperone located in the endoplasmic reticulum, affects cell-substratum adhesions via the induction of vinculin and N-cadherin. Cells overexpressing calreticulin contain more vinculin than low expressers and make abundant contacts ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20060105
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND INFORMATION:There are several reports indicating that starved fibroblasts show higher proliferation rates when re-fed with foetal bovine serum. We have evidence demonstrating that this phenomenon is related to secretory proteins which may be beneficial to wound healing. RESULTS:After re-feeding, 16 and 72 h...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201300063
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND INFORMATION:The mitogen-inducible gene-6 (Mig-6) is a non-kinase scaffolding adaptor protein. It has been shown that Mig-6 may play important roles in regulating stress response, maintaining homeostasis and functioning as a tumour suppressor. In this study, we investigated the role of Mig-6 in cellular senes...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201200052
更新日期:2013-10-01 00:00:00
abstract:BACKGROUND INFORMATION:Carcinoma of the oesophagus is the sixth leading cause of cancer death in the western world and is associated with a 5-year survival of less than 15%. Recent evidence suggests that stromal-epithelial interactions are fundamental in carcinogenesis. The advent of co-culture techniques permits the i...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20100071
更新日期:2010-12-01 00:00:00
abstract::The ciliated protozoan Paramecium harbours a particularly large diversity of microtubule networks, ranging from the elaborate and stable ciliary axonemes and basal bodies to very dynamic cytoplasmic, cortical or intranuclear arrays. Their organization and individual cycle of assembly/disassembly are well known and ext...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(97)89840-1
更新日期:1996-01-01 00:00:00
abstract::A central issue in the understanding of Marfan syndrome deals with the functional architecture of fibrillin-containing microfibrils. Fibrillin-rich microfibrils are long extracellular matrix fibrillar components exhibiting a 50 nm periodic beaded-structure with a width of around 20-25 nm after rotary shadowing and a 1...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(98)80018-x
更新日期:1998-06-01 00:00:00
abstract:BACKGROUND INFORMATION:The rod outer segment (OS) is the specialised organelle where phototransduction takes place. Our previous proteomic and biochemical analyses on purified rod disks showed the functional expression of the respiratory chain complexes I-IV and F1 Fo -ATP synthase in OS disks, as well as active solubl...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201300003
更新日期:2013-08-01 00:00:00
abstract::In spite of the constant development concerning physical mapping of eukaryotic genomes, the mouse chromosome 19 remains poorly characterized. In order to improve the possibilities for studying this chromosome, we have constructed a chromosome-specific EcoRI DNA fragment library from mouse chromosomes 19 sorted by flow...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90322-t
更新日期:1990-01-01 00:00:00
abstract::Extensive developmentally programmed DNA rearrangements, including thousands of internal deletions, occur in the differentiating somatic macronucleus in Tetrahymena thermophila. Some deletion systems involve the use of multiple alternative deletion sites. We report here the cloning and the sequences of three new alter...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(96)84773-3
更新日期:1996-01-01 00:00:00
abstract::Antidiuretic hormone induces, in the apical plasma membrane of amphibian urinary bladder epithelial cells, the exocytotic insertion of intramembranous particle aggregates that probably contain water channels. Purification of the apical membrane is a way to characterize the aggregates. The isolation of such purified me...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::The interaction of cells with extracellular matrix components contributes to their specific differentiation. We studied hepatic peroxisomes and their changing features during embryonic development, and we immunolocalized in the same tissue the extracellular matrix components laminin and collagen IV as well as the inte...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::Oocyte maturation and early development have been intensively studied for well over 100 years. The earliest theory proposed that after fertilisation and during cell division determinants were unequally distributed to control cell fate; experimental proof came from using frog eggs (Roux, 1888). After understanding the ...
journal_title:Biology of the cell
pub_type: 传,社论,历史文章
doi:10.1016/j.biolcel.2004.01.002
更新日期:2004-04-01 00:00:00
abstract:BACKGROUND INFORMATION:AQP10 (aquaporin 10) is a new member of water channels and two different transcripts have been reported. The difference between these transcripts is in the presence or the absence of an insertion of 475 nt, which leads to a translation frame shift resulting in a different termination of amino aci...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20040091
更新日期:2005-11-01 00:00:00