Relationship between microtubules and Golgi apparatus in hepatocytes: a quantitative study during experimental nephrosis.

abstract：:Detection of nucleic acid sequence at the ultrastructural level has allowed us to better understand the expression of genes in some fields of application in cell biology. In situ hybridization at the ultrastructural level can be carried out using three different methods: on vibratome sections before embedding in epoxy...

journal_title：Biology of the cell

authors： Le Guellec D

更新日期：1998-07-01 00:00:00

abstract：:We have identified and characterized a membrane glycoprotein located at the apical plasma membrane of adult human colon epithelial cells, by the use of the monoclonal antibody technique in combination with immunocytochemical and biochemical methods. Analysis of membranes extracted with Triton X-114 and treated with sp...

journal_title：Biology of the cell

authors： Le Bivic A,Hirn M,Reggio H

更新日期：1987-01-01 00:00:00

abstract：BACKGROUND INFORMATION:The results of water permeability measurements suggest the presence of an AQP (aquaporin) in the membrane of the CV (contractile vacuole) in Amoeba proteus [Nishihara, Shimmen and Sonobe (2004) Cell Struct. Funct. 29, 85-90]. RESULTS:In the present study, we cloned an AQP gene from A. proteus [A...

journal_title：Biology of the cell

authors： Nishihara E,Yokota E,Tazaki A,Orii H,Katsuhara M,Kataoka K,Igarashi H,Moriyama Y,Shimmen T,Sonobe S

更新日期：2008-03-01 00:00:00

abstract：:LDs (lipid droplets) have long been considered as inert particles used by the cells to store fatty acids and sterols as esterified non-toxic lipid species (i.e. triacylglycerols and steryl esters). However, accumulating evidence suggests that LDs behave as a dynamic compartment, which is involved in the regulation of ...

journal_title：Biology of the cell

authors： Hapala I,Marza E,Ferreira T

更新日期：2011-06-01 00:00:00

abstract：:The activation of M-phase promoting factor (MPF) in one-cell mouse embryo is independent from the nucleus. Other autonomous phenomena include the cortical activity observed at the end of the first cell cycle and the reorganization of the microtubule network. Here, we observed that the autonomous control of MPF activat...

journal_title：Biology of the cell

authors： Ciemerych MA,Tarkowski AK,Kubiak JZ

更新日期：1998-11-01 00:00:00

abstract：BACKGROUND INFORMATION:AQP10 (aquaporin 10) is a new member of water channels and two different transcripts have been reported. The difference between these transcripts is in the presence or the absence of an insertion of 475 nt, which leads to a translation frame shift resulting in a different termination of amino aci...

journal_title：Biology of the cell

authors： Li H,Kamiie J,Morishita Y,Yoshida Y,Yaoita E,Ishibashi K,Yamamoto T

更新日期：2005-11-01 00:00:00

abstract：:The first step of polyamine uptake is the binding of polyamines to the cell membrane. In order to characterize the specificity of the putrescine binding sites at the surface of the glioblastoma cells (U251), we have carried out competition experiments between putrescine bound to latex microspheres and vizualized by sc...

journal_title：Biology of the cell

authors： Quemener V,Moulinoux JP,Khan NA,Seiler N

更新日期：1990-01-01 00:00:00

abstract：:ESCRTs (endosomal sorting complexes required for transport) were first discovered in yeast and are known to be required in the biogenesis of the MVB (multivesicular body). Most ESCRT research has been carried out in vitro using models such as yeast and mammalian cells in culture. The role of the ESCRTs genes in endoso...

journal_title：Biology of the cell

authors： Michelet X,Djeddi A,Legouis R

更新日期：2010-01-13 00:00:00

abstract：:The study reports morphological growth characteristics and chromosome analysis of neoplastic rat pleural mesothelial cells (RPMC) isolated from a mesothelioma-bearing rat. The pleural mesothelioma was induced by intrapleural injection of chrysotile fibers. Neoplastic RPMC were cultured by the standard methods used for...

journal_title：Biology of the cell

authors： Jaurand MC,Renier A,Van der Meeren A,Magne L,Pinchon MC,Bignon J

更新日期：1986-01-01 00:00:00

abstract：:The activities of three mycotoxins isolated from the hyphomycete Metarhizium anisopliae: destruxin A, B, and E (DA, DB and DE) are described and compared in vitro on leukemic cells. Their antitumor effect was investigated by flow cytometry on growth, cell viability and cell cycle perturbation 48 h after destruxin expo...

journal_title：Biology of the cell

authors： Odier F,Vey A,Bureau JP

更新日期：1992-01-01 00:00:00

abstract：:It is now well established that malignant transformation of eucaryotic cells is concomitant with typical alterations of glycosylation and the expression pattern of endogenous lectins. In parallel, oncogene transfection studies revealed a correlation between the expression of some of these genes, the transformed state ...

journal_title：Biology of the cell

authors： Hébert E,Monsigny M

更新日期：1993-01-01 00:00:00

abstract：:One of the initial events required for the expression of cartilage-specific macromolecules in monolayer cultures is the reversion to the initial round shape of chondrocytes. Thus, considerable research efforts have focused on developing reliable procedures to maintain a round morphology of cultured chondrocytes. Our s...

journal_title：Biology of the cell

authors： Loty S,Forest N,Boulekbache H,Sautier JM

更新日期：1995-01-01 00:00:00

abstract：:Interactions of cells with the extracellular matrix are essential for the control of tissue remodelling, cell migration, and embryogenesis. At the cell-extracellular matrix contact points, specialized structures are formed and termed focal adhesions, where transmembrane adhesion receptors provide a structural link bet...

journal_title：Biology of the cell

authors： Petit V,Thiery JP

更新日期：2000-10-01 00:00:00

abstract：:The location of lipopolysaccharide (LPS) was studied by immunofluorescence and immunoelectron microscopy in macrophages infected with a non-invasive Shigella dysenteriae 1 strain. Bacterial degradation began only 3 h after the end of infection. The first visible sign of degradation was detected by immunogold labelling...

journal_title：Biology of the cell

authors： Dacosta B,Ryter A,Mounier J,Sansonetti P

更新日期：1990-01-01 00:00:00

abstract：:Using gold labelled neoglycoproteins containing either alpha-D-glucose, N-acetyl-beta-D-glucosamine, alpha-D-mannose, 6-phospho-alpha-D-mannose, and alpha-L-fucose (BSA), we investigated their intranuclear binding sites in the TG human cell line. Although gold-labelled BSA did not give any noticeable labelling, the pr...

journal_title：Biology of the cell

authors： Bourgeois CA,Monsigny M

更新日期：1990-01-01 00:00:00

abstract：:Oocyte maturation and early development have been intensively studied for well over 100 years. The earliest theory proposed that after fertilisation and during cell division determinants were unequally distributed to control cell fate; experimental proof came from using frog eggs (Roux, 1888). After understanding the ...

journal_title：Biology of the cell

authors： Prigent C,Hunt T

更新日期：2004-04-01 00:00:00

abstract：:Lentil seedlings were grown for 28 h in space, on board Spacelab (IML 1 Mission) and growth of the primary root was analysed. The length of cortical cells was less in near weightlessness than on the 1 g centrifuge (flight control) and mitotic index was lower but there was no apparent perturbation in the mitosis. To fu...

journal_title：Biology of the cell

authors： Driss-Ecole D,Schoevaert D,Noin M,Perbal G

更新日期：1994-01-01 00:00:00

abstract：:ERM (ezrin/radixin/moesin) proteins, concentrated in actin rich cell-surface structures, cross-link actin filaments with the plasma membrane. They are involved in the formation of microvilli, cell-cell adhesion, maintenance of cell shape, cell motility and membrane trafficking. Recent analyses reveal that they are not...

journal_title：Biology of the cell

authors： Louvet-Vallée S

更新日期：2000-08-01 00:00:00

abstract：:The clonal rat rhabdomyosarcoma cell line BA-HAN-1C is composed of proliferating mononuclear cells, some of which spontaneously fuse to terminally differentiated myotube-like giant cells. This cell line has been shown to be susceptible to differentiation induction with all-trans retinoic acid (RA). Since it is still u...

journal_title：Biology of the cell

authors： Ramp U,Gerharz CD,Eifler E,Biesalski HK,Gabbert HE

更新日期：1994-01-01 00:00:00

abstract：:Evidence is presented that two circumferential zones of cell envelope differentiation, the periseptal annuli, exist in E. coli as previously observed in S. typhimurium. The periseptal annulus is located at the division site of cells. A strain overproducing a periplasmic protein, PhoS (phosphate-binding protein) has be...

journal_title：Biology of the cell

authors： Anba J,Bernadac A,Pages JM,Lazdunski C

更新日期：1984-01-01 00:00:00

abstract：:A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human ce...

journal_title：Biology of the cell

authors： Philipova RN,Zhelev NZ,Todorov IT,Hadjiolov AA

更新日期：1987-01-01 00:00:00

abstract：:Regulated exocytosis of defensive secretory organelles, the trichocysts, as well as a transient Ca(2+)-influx can be induced in Paramecium by aminoethyldextran (Kerboeuf and Cohen, J Cell Biol (1990) 111, 2527). Knoll et al (Febs Lett (1992) 304, 265) reported that veratridine was also a secretagogue for Paramecium. H...

journal_title：Biology of the cell

authors： Kerboeuf D,Cohen J

更新日期：1996-01-01 00:00:00

abstract：:Xenopus oocytes were stained by anti-tubulin and anti-MAP1 antibodies during the first meiotic cell division. In the prophase-blocked oocytes, only few microtubules are present around the upper part of the nuclear envelope. These microtubules are resistant to cold, calcium and antimitotic drug treatments. At this stag...

journal_title：Biology of the cell

authors： Jessus C,Huchon D,Ozon R

更新日期：1986-01-01 00:00:00

abstract：:Flow cytometry (FCM) analysis of cellular DNA content has become an increasingly important clinical research tool for the measurement and identification of abnormal cell populations. Examination of cellular DNA content can provide information for use in cell cycle analysis in cancer studies. Most researchers would agr...

journal_title：Biology of the cell

authors： Spyratos F

更新日期：1993-01-01 00:00:00

abstract：BACKGROUND INFORMATION:Substantial evidence indicates the existence of NCSCs (neural crest-derived stem cells) in embryonic mandibular processes; however, they have not been fully investigated or isolated. The aim of the present study was to isolate stem cells from mandibular process during embryonic development by MAC...

journal_title：Biology of the cell

authors： Zhang J,Duan X,Zhang H,Deng Z,Zhou Z,Wen N,Smith AJ,Zhao W,Jin Y

更新日期：2006-10-01 00:00:00

abstract：:The aim of a large number of studies on G protein-coupled receptors was centered on understanding the structural basis of their main functional properties. Here, we will briefly review the results obtained on the alpha1-adrenergic receptor subtypes belonging to the rhodopsin-like family of receptors. These findings co...

journal_title：Biology of the cell

authors： Cotecchia S,Stanasila L,Diviani D,Björklöf K,Rossier O,Fanelli F

更新日期：2004-06-01 00:00:00

abstract：BACKGROUND INFORMATION:The endometrial epithelial cell membrane is a key interface in female reproductive biology. Steroid hormones play a predominant role in cyclic changes which occur at this interface during the female menstrual cycle. Specific changes in the morphology of the endometrial epithelial cell surface bec...

journal_title：Biology of the cell

authors： Francis LW,Lewis PD,Gonzalez D,Ryder TA,Webb G,Joels LA,White JO,Wright CJ,Conlan RS

更新日期：2009-06-04 00:00:00

abstract：BACKGROUND INFORMATION:AQP4 (aquaporin 4) internalization and a concomitant decrease in the osmotic water permeability coefficient (Pf) after histamine exposure has been reported in AQP4-transfected gastric HGT1 cells. RESULTS:In the present study we report that AQP4 internalization is followed by an increase in AQP4 ...

journal_title：Biology of the cell

authors： Carmosino M,Procino G,Tamma G,Mannucci R,Svelto M,Valenti G

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND INFORMATION:NPY (neuropeptide Y) may have an effect on the properties of vascular endothelial cells such as pro-angiogenic effects and potentiation of noradrenaline-induced vasoconstriction. In HUVEC (human umbilical-vein endothelial cells), immunoreactive neuropeptide Y has been detected, but NPY synthesis,...

journal_title：Biology of the cell

authors： Silva AP,Kaufmann JE,Vivancos C,Fakan S,Cavadas C,Shaw P,Brunner HR,Vischer U,Grouzmann E

更新日期：2005-06-01 00:00:00

abstract：:The chromosomal localization of the mouse gene coding for the 68 kDa intermediate filament subunit of neurones (NF-L) was determined by in situ hybridization using specific 3H-labelled DNA probes. There is only one copy of the NF-L gene. The gene encoding NF-L is located on chromosome 14 region (D1-E1). ...

journal_title：Biology of the cell

authors： Mattei MG,Duprey P,Li ZL,Mattei JF,Paulin D

更新日期：1989-01-01 00:00:00