Abstract:
BACKGROUND INFORMATION:The identification of a source of stem cells able to regenerate skeletal muscle was the goal of numerous studies with the aim to develop new therapeutic approaches for genetic muscle diseases or muscle injuries. A series of studies have demonstrated that stem cells derived from various tissues may have a role in the regeneration of damaged muscles, but this contribution is always very weak. Thus we established a project aiming to reprogramme non-muscle cells into the skeletal striated differentiation pathway. RESULTS:We transduced several human primary adult stem or progenitor cells using a recombinant lentivirus containing the coding sequence of the Myf5 gene considered as a master gene for the determination of skeletal striated muscle. These original results are the first demonstration of a myogenic conversion of human mesenchymal and endothelial cells by Myf5. CONCLUSIONS:The procedure described in the present paper could be used to develop new research protocols with the prospect of using these cells as therapeutic agents.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Dimicoli-Salazar S,Bulle F,Yacia A,Massé JM,Fichelson S,Vigon Idoi
10.1042/BC20100112subject
Has Abstractpub_date
2011-11-01 00:00:00pages
531-42issue
11eissn
0248-4900issn
1768-322Xpii
BC20100112journal_volume
103pub_type
杂志文章abstract::It is now well established that malignant transformation of eucaryotic cells is concomitant with typical alterations of glycosylation and the expression pattern of endogenous lectins. In parallel, oncogene transfection studies revealed a correlation between the expression of some of these genes, the transformed state ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1111/j.1768-322x.1993.tb00899.x
更新日期:1993-01-01 00:00:00
abstract::Interaction of sperm and egg at fertilization induces well-coordinated molecular events including specific recognition between species, adhesion and fusion, that lead to the formation of a zygote, a totipotent cell that develops into a new individual. A calcium signal, common to a great number of species, from marine ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(00)01065-0
更新日期:2000-07-01 00:00:00
abstract::A digitally-enhanced videomicroscopy study of rabbit gastric parietal cells in primary culture was performed using alternate observations with differential interference contrast and fluorescence optics of cells mounted and perfused on a temperature-controlled microscope stage. The effect of histamine, a physiological ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90349-8
更新日期:1990-01-01 00:00:00
abstract::The lateral line of the zebrafish has many of the advantages that made the sensory organs of Drosophila a very productive model system: 1) it comprises a set of discrete sense organs (neuromasts) arranged in a defined, species-specific pattern, such that each organ can be individually recognized; 2) the neuromasts are...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/j.biolcel.2003.10.005
更新日期:2003-12-01 00:00:00
abstract::Extensive developmentally programmed DNA rearrangements, including thousands of internal deletions, occur in the differentiating somatic macronucleus in Tetrahymena thermophila. Some deletion systems involve the use of multiple alternative deletion sites. We report here the cloning and the sequences of three new alter...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(96)84773-3
更新日期:1996-01-01 00:00:00
abstract::Ultrastructural morphometry (point-counting method) was used to evaluate stereological parameters of rough endoplasmic reticulum (RER), Golgi complex, secretory granules, mitochondria and lysosomes of Rana ridibunda pituitary corticotropic (ACTH) cells in animals subjected to constant environmental conditions (CEC) in...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00472.x
更新日期:1986-01-01 00:00:00
abstract::We took advantage of one of the main possibilities of ion microscopy, ie isotopic analysis, to study the cellular distribution of molecules labelled either with carbon 14 or with stable isotopes of low natural abundance such as nitrogen 15 and deuterium. The surface of the sample is bombarded with an ion beam (O2+, Cs...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(92)90011-o
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND INFORMATION:MTA1 (metastasis-associated gene 1) has been reported to be overexpressed in cancers with high potential to metastasize. Studies of the molecular mechanisms revealed that MTA1 plays an important role in the process of metastasis of many types of cancer. However, the role of MTA1 in melanoma devel...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/bc20060130
更新日期:2007-10-01 00:00:00
abstract::The internalization of the yolk proteins has been investigated by electron microscopy and cytochemistry in the oocyte of the trout which stores up large quantities of yolk. The oocyte evolution has been followed for 18 months in a homogeneous group of animals. Anionic ferritin has been injected during vitellogenesis. ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00283.x
更新日期:1984-01-01 00:00:00
abstract:BACKGROUND INFORMATION:The interferon (IFN)-inducible protein TRIM22 (Staf50) is a member of the tripartite motif protein family and has been suggested a role in the regulation of viral replication as well as of protein ubiquitylation. In addition, we have previously shown that TRIM22 is a direct target gene for the tu...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201100099
更新日期:2012-08-01 00:00:00
abstract::Inside-out porcine thyroid follicles in culture undergo polarity reversal after being embedded in collagen gel. The newly-formed follicles reexpress some specific thyroid functions lost in inside-out follicles (Chambard et al., 1984. We present here an ultrastructural study of the inversion of polarity in this model s...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00463.x
更新日期:1986-01-01 00:00:00
abstract::This study was undertaken to evaluate whether hypertrophy and hyperplasia of the pancreatic acinar cells induced by caerulein remained after termination of the hormonal treatment. Rats received subcutaneous injections of saline or caerulein for 4 days and were killed immediately after termination of treatment or 2, 15...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND INFORMATION:Human gingival tissues are prone to hyperplasia under inflammatory stimuli. We have identified gingival tissue-specific mesenchymal stem cells (GMSCs) and found their functional change being correlated with drug-induced gingival hyperplasia. However, whether these cells exhibit characteristics of...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201200064
更新日期:2013-06-01 00:00:00
abstract::Gap junctions are widely expressed in the various cell types of the central nervous system. These specialized membrane intercellular junctions provide the morphological support for direct electrical and biochemical communication between adjacent cells. This intercellular coupling is controlled by neurotransmitters and...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(02)00016-3
更新日期:2002-11-01 00:00:00
abstract::Isolated cells from the proximal tubule have previously been used to study specific renal physiological processes. Here we analysed the structural changes induced by mechanical isolation on rat proximal tubule cells. As major modifications we detected: 1) a redistribution of brush border microvilli, which spread out o...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(92)90026-w
更新日期:1992-01-01 00:00:00
abstract::Serial sections analysis of spermatocytes of the rodent Phyllotis darwini, reveal a persistent positional relationship between nucleolar material and centrioles. Pore rich areas interposed between these nucleocytoplasmic components, are represented graphically. Centriole replication takes place during the pachytene st...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00266.x
更新日期:1984-01-01 00:00:00
abstract::The activation of M-phase promoting factor (MPF) in one-cell mouse embryo is independent from the nucleus. Other autonomous phenomena include the cortical activity observed at the end of the first cell cycle and the reorganization of the microtubule network. Here, we observed that the autonomous control of MPF activat...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract:BACKGROUND INFORMATION:Substantial evidence indicates the existence of NCSCs (neural crest-derived stem cells) in embryonic mandibular processes; however, they have not been fully investigated or isolated. The aim of the present study was to isolate stem cells from mandibular process during embryonic development by MAC...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20060012
更新日期:2006-10-01 00:00:00
abstract::In cold-stressed oocytes of Pleurodeles waltl, lampbrush chromosome lateral loops exhibited important structural modifications which were visualized under light microscopy. Electron microscopy study revealed that the RNP particles associated with growing transcripts in the matrix of these loops were 15 nm at 8 degrees...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90340-9
更新日期:1990-01-01 00:00:00
abstract::We studied mechanisms that mediate recognition of human erythrocytes (HRBC) and sheep erythrocytes (SRBC) by rat liver macrophages. We used an in vitro cell binding assay that allows spontaneous formation of cell contacts. Binding of HRBC to rat macrophages shows the following characteristics: inhibition studies with ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1987.tb00558.x
更新日期:1987-01-01 00:00:00
abstract::Several transformed cells have been demonstrated to secrete growth factors. We studied the effect of conditioned medium from neoplastic rat pleural mesothelial cells on normal and neoplastic mesothelial cell growth. The results showed that the concentrated conditioned medium stimulated neoplastic mesothelial cell grow...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(88)90025-1
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND INFORMATION:Our previous studies have shown that calreticulin, a Ca2+-binding chaperone located in the endoplasmic reticulum, affects cell-substratum adhesions via the induction of vinculin and N-cadherin. Cells overexpressing calreticulin contain more vinculin than low expressers and make abundant contacts ...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20060105
更新日期:2007-07-01 00:00:00
abstract::In order to determine whether the human lactotransferrin receptor recently described on platelets was also present on hematopoietic precursors, we investigated its presence and characteristics on the megakaryocytic Dami cell line. The reversible binding of human 5-(([2-(carbo(hydrazino)methyl]thio)acetyl)aminofluoresc...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(94)80017-0
更新日期:1994-01-01 00:00:00
abstract::beta-Catenin was initially characterized as a protein interacting with the cadherin cytoplasmic tail and regulating cell-cell contacts and actin cytoskeleton interactions. Moreover, the gene coding for the Drosophila orthologue of beta-catenin, armadillo, was independently identified downstream of wingless in the segm...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20040531
更新日期:2005-12-01 00:00:00
abstract::Human pancreatic ductal cells of the Capan 1 cell line differentiate progressively during growth. After the exponential growth phase, the cells elongate and become polarized with their apical poles covered by microvilli and separated from the basolateral pole by tight junctions. In this stationary phase, they form dom...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(94)80004-9
更新日期:1994-01-01 00:00:00
abstract::The highly conserved VICKZ (Vg1 RBP/Vera, IMP-1,2,3, CRD-BP, KOC, ZBP-1) family of RNA-binding proteins recognize specific cis-acting elements in a variety of different RNAs and have been implicated in cell polarity and migration, cell proliferation, and cancer. In just the last two years, the use of transgenic mice, ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20040151
更新日期:2005-01-01 00:00:00
abstract::The influence of triiodothyronine (T3) on avian myoblast proliferation and differentiation was studied in secondary cultures using plating densities of 2,500 and 7,000 cells/cm2. Culture media were depleted of T3 (control myoblasts) and increasing amounts were then added to concentrations of 0.6, 3 and 15 nM T3 (treat...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(93)90129-3
更新日期:1993-01-01 00:00:00
abstract::Co-isolated residual nuclear shells and residual nucleoli from membrane-depleted rat liver nuclei were spread according to Kleinschmidt's method. Comparison of the spread residual structures isolated from nuclear shells and spread pore complex-lamina isolated from nuclear envelopes showed that these residual structure...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00438.x
更新日期:1986-01-01 00:00:00
abstract::I am pleased to contribute to this special issue of Biology of the Cell in honor of Yoshio Masui. Oocyte maturation remains a small enough and young enough field that the authors assembled for this issue can trace the entire development of the field over the last 30 years, beginning with the early demonstration by Mas...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:
更新日期:1998-10-01 00:00:00
abstract:BACKGROUND INFORMATION:αvβ5 integrin and Mer tyrosine kinase (MerTK) receptors reside at the apical surface of the retinal pigment epithelium (RPE) in the eye to promote the diurnal, synchronised phagocytosis of shed photoreceptor outer segment fragments (POS) that is critical for vision. Phagocytosis assays studying R...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201100076
更新日期:2012-06-01 00:00:00