Efficient in vitro myogenic reprogramming of human primary mesenchymal stem cells and endothelial cells by Myf5.

Abstract:

BACKGROUND INFORMATION:The identification of a source of stem cells able to regenerate skeletal muscle was the goal of numerous studies with the aim to develop new therapeutic approaches for genetic muscle diseases or muscle injuries. A series of studies have demonstrated that stem cells derived from various tissues may have a role in the regeneration of damaged muscles, but this contribution is always very weak. Thus we established a project aiming to reprogramme non-muscle cells into the skeletal striated differentiation pathway. RESULTS:We transduced several human primary adult stem or progenitor cells using a recombinant lentivirus containing the coding sequence of the Myf5 gene considered as a master gene for the determination of skeletal striated muscle. These original results are the first demonstration of a myogenic conversion of human mesenchymal and endothelial cells by Myf5. CONCLUSIONS:The procedure described in the present paper could be used to develop new research protocols with the prospect of using these cells as therapeutic agents.

journal_name

Biol Cell

journal_title

Biology of the cell

authors

Dimicoli-Salazar S,Bulle F,Yacia A,Massé JM,Fichelson S,Vigon I

doi

10.1042/BC20100112

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

531-42

issue

11

eissn

0248-4900

issn

1768-322X

pii

BC20100112

journal_volume

103

pub_type

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