Abstract:
BACKGROUND INFORMATION:Substantial evidence indicates the existence of NCSCs (neural crest-derived stem cells) in embryonic mandibular processes; however, they have not been fully investigated or isolated. The aim of the present study was to isolate stem cells from mandibular process during embryonic development by MACS (magnetic-activated cell sorting). The findings show that the cells are multipotent and self-renewing. RESULTS:LNGFR (low-affinity nerve-growth-factor receptor)+ cells were isolated from rat embryonic mandibular processes by MACS. The cells were grown in clonal culture by limiting dilution to assess their developmental potential. Clone analysis indicated that, first, LNGFR+ cells are multipotent, being able to generate at least neurons and Schwann cells, similar to peripheral neural crest stem cells. Secondly, multipotent LNGFR+ cells generate multipotent progenies, indicating that they are capable of self-renewal and therefore are stem cells. Thirdly, manipulation of the medium supplementation alters the fate of the isolated LNGFR+ cells. CONCLUSIONS:These results indicate that LNGFR antibodies label NCSCs with high specificity and purity, and suggest that positive selection using these antibodies may become the method of choice for obtaining multipotent cells from rat embryonic mandibular processes for tissue engineering or regenerative therapeutic use.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Zhang J,Duan X,Zhang H,Deng Z,Zhou Z,Wen N,Smith AJ,Zhao W,Jin Ydoi
10.1042/BC20060012subject
Has Abstractpub_date
2006-10-01 00:00:00pages
567-75issue
10eissn
0248-4900issn
1768-322Xpii
BC20060012journal_volume
98pub_type
杂志文章abstract::Retinitis pigmentosa (RP) is a heterogeneous group of inherited retinal degenerative diseases, characterized by the progressive death of rod and cone photoreceptors. A tremendous genetic heterogeneity is associated with the RP phenotype. Most mutations affect rods selectively and, through an unknown pathway, cause the...
journal_title:Biology of the cell
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abstract::Several transformed cells have been demonstrated to secrete growth factors. We studied the effect of conditioned medium from neoplastic rat pleural mesothelial cells on normal and neoplastic mesothelial cell growth. The results showed that the concentrated conditioned medium stimulated neoplastic mesothelial cell grow...
journal_title:Biology of the cell
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abstract::cdc25 proteins are universally involved in the control of cell division. Using an original method of sequence analysis, cdc25 proteins from different sources were compared to protein phosphatases. Protein phosphatases could clearly be characterized as two distinct protein families, the phospho-seryl/threonyl phosphata...
journal_title:Biology of the cell
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doi:10.1016/0248-4900(92)90134-m
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journal_title:Biology of the cell
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更新日期:1992-01-01 00:00:00
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journal_title:Biology of the cell
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doi:10.1016/0248-4900(90)90322-t
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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更新日期:2015-09-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1984.tb00309.x
更新日期:1984-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
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更新日期:2003-11-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章,评审
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更新日期:1998-10-01 00:00:00
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1985.tb00431.x
更新日期:1985-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(91)90054-q
更新日期:1991-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20080060
更新日期:2009-04-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/0248-4900(93)90119-y
更新日期:1993-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/s0248-4900(99)80078-1
更新日期:1997-03-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90362-7
更新日期:1990-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1042/BC20040102
更新日期:2005-06-01 00:00:00
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更新日期:2006-08-01 00:00:00
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journal_title:Biology of the cell
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doi:
更新日期:1999-05-01 00:00:00
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journal_title:Biology of the cell
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doi:
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1985.tb00375.x
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/boc.201100099
更新日期:2012-08-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章,评审
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更新日期:2008-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(92)90200-k
更新日期:1992-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
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journal_title:Biology of the cell
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doi:
更新日期:1997-08-01 00:00:00
abstract::The ultrastructure of the Drosophila subobscura chromosome regions around the breakpoints of the complex E1 + 2 + 9 + 12 gene arrangement was analyzed. This overlapping inversion is formed by the association of the E1, E2, E9, and E12 simple inversions. Ultrastructure of sections involving 58D/59A, 61C/D, 62D/63A, 64B...
journal_title:Biology of the cell
pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00