Abstract:
:Isolated cells from the proximal tubule have previously been used to study specific renal physiological processes. Here we analysed the structural changes induced by mechanical isolation on rat proximal tubule cells. As major modifications we detected: 1) a redistribution of brush border microvilli, which spread out over all the cell surface immediately after isolation; 2) a rapid redistribution of the apical membrane enzyme markers aminopeptidase N and isomaltase; and 3) a striking decrease of membrane surface amplification factor. The results show that cells isolated from the proximal tubule rapidly loose their characteristic polarized membrane structure. Loss of epithelial polarity implies loss of several vectorial transports and should thus be cautiously taken into account when working with this model system.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Rebelo L,Carmo-Fonseca M,Moura TFdoi
10.1016/0248-4900(92)90026-wsubject
Has Abstractpub_date
1992-01-01 00:00:00pages
203-9issue
2eissn
0248-4900issn
1768-322Xjournal_volume
74pub_type
杂志文章abstract::Circulating glucocorticoid (GC) levels are thought to modulate the basal activity of pro-opiomelanocortin (POMC) neurons within the mediobasal hypothalamus (MBH) of the male rat. In a recent study we demonstrated that Fos-immunoreactivity (Fos-IR) was spontaneously induced throughout the dark phase of the light/dark c...
journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1016/s0248-4900(00)01083-2
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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doi:10.1042/BC20060105
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journal_title:Biology of the cell
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更新日期:2004-02-01 00:00:00
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journal_title:Biology of the cell
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doi:10.1111/j.1768-322x.1986.tb00463.x
更新日期:1986-01-01 00:00:00
abstract::Xenopus oocytes were stained by anti-tubulin and anti-MAP1 antibodies during the first meiotic cell division. In the prophase-blocked oocytes, only few microtubules are present around the upper part of the nuclear envelope. These microtubules are resistant to cold, calcium and antimitotic drug treatments. At this stag...
journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00448.x
更新日期:1986-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
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journal_title:Biology of the cell
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更新日期:2008-07-01 00:00:00
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journal_title:Biology of the cell
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doi:10.1016/0248-4900(88)90072-x
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journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1111/j.1768-322x.1993.tb00899.x
更新日期:1993-01-01 00:00:00
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1985.tb00431.x
更新日期:1985-01-01 00:00:00
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journal_title:Biology of the cell
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doi:10.1111/j.1768-322x.1986.tb00481.x
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(88)90002-0
更新日期:1988-01-01 00:00:00
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journal_title:Biology of the cell
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1111/j.1768-322x.1986.tb00487.x
更新日期:1986-01-01 00:00:00
abstract::Since almost two decades, it is known that progesterone is responsible of the release of the prophase I arrest of amphibian oocytes and leads to the activation of the universal MPF, through a puzzling transduction pathway. It involves negative regulation of the cAMP-dependent protein kinase (PKA) and synthesis of new ...
journal_title:Biology of the cell
pub_type: 杂志文章,评审
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journal_title:Biology of the cell
pub_type: 杂志文章
doi:10.1016/0248-4900(90)90340-9
更新日期:1990-01-01 00:00:00
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journal_title:Biology of the cell
pub_type: 杂志文章,评审
doi:10.1042/BC20060048
更新日期:2006-11-01 00:00:00
abstract:BACKGROUND INFORMATION:The BOR (branchio-oto-renal) syndrome is a dominant disorder most commonly caused by mutations in the EYA1 (Eyes Absent 1) gene. Symptoms commonly include deafness and renal anomalies. RESULTS:We have used the embryos of the frog Xenopus laevis as an animal model for early ear development to exa...
journal_title:Biology of the cell
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doi:10.1042/BC20090098
更新日期:2010-02-17 00:00:00