Abstract：
:Xenopus oocytes were stained by anti-tubulin and anti-MAP1 antibodies during the first meiotic cell division. In the prophase-blocked oocytes, only few microtubules are present around the upper part of the nuclear envelope. These microtubules are resistant to cold, calcium and antimitotic drug treatments. At this stage, monoclonal anti-MAP1 antibody and polyclonal anti-centrosome antibody reveal punctate staining of the nucleus and nucleoli. During the progesterone-induced maturation, a microtubular network appears at the basal part of the disrupting nucleus. Anti-MAP1 and anti-centrosome antibodies stain a dense layer at the basal part of this microtubular array. Microtubules present in this array are cold, calcium- and antimitotic drug sensitive. Anti-MAP1 and anti-tubulin antibodies stain the whole metaphase II spindle, whereas only the poles of the metaphase II spindle are stained by the anti-centrosome antibody.
journal_name
Biol Celljournal_title
Biology of the cellauthors
Jessus C,Huchon D,Ozon Rdoi
10.1111/j.1768-322x.1986.tb00448.xsubject
Has Abstractpub_date
1986-01-01 00:00:00pages
113-20issue
2eissn
0248-4900issn
1768-322Xjournal_volume
56pub_type
杂志文章abstract：:Aspergillus fumigatus, the causative agent of human aspergillosis, binds to and degrades basement membrane laminin. Using immunoelectron microscopy, laminin binding appeared to be associated with the cell wall expansions of resting conidia, and progressively extended to the outer electron dense layer of the conidial w...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(05)80189-3
更新日期：1993-01-01 00:00:00
abstract：:The isolation, characterization, and expression of a novel cDNA encoding a Trypanosoma cruzi polypeptide (TcAc2), homologous to various small stress proteins and glutathione S-transferases, are described. The deduced amino-acid sequence revealed two domains sharing 27% identity and an additional 27% similarity to each...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(94)90011-6
更新日期：1994-01-01 00:00:00
abstract：:The major finding of the present study is that the ultrastructural organization of the neuromuscular synapse can be modified by a small, 4-week-long, physiological increase in the locomotor activity of the extensor digitorum longus muscle of normal adult rats trained to walk. This study measures these plastic adaptati...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(99)80078-1
更新日期：1997-03-01 00:00:00
abstract：BACKGROUND INFORMATION:ROS (reactive oxygen species) as well as components of the antioxidant redox systems may act as signals. To link acute environmental change with gene expression, changes in ROS and GSH/GSSG (reduced/oxidized glutathione) level were measured upon acute changes in temperature or oxygen availability...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20100145
更新日期：2011-08-01 00:00:00
abstract：:During wound-healing in cultured frog skin fragments, fibronectin (FN) was detected in the dermal-epidermal junction. Intracellular fibronectin was stained using permeabilization and DAB immunoperoxidase. With electron microscopy intracytoplasmic FN granules were localized in the epidermal processes of the stratum ger...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1989.tb03013.x
更新日期：1989-01-01 00:00:00
abstract：:Cells sense and respond to the biochemical and physical properties of the extracellular matrix (ECM) through adhesive structures that bridge the cell cytoskeleton and the surrounding environment. Integrin-mediated adhesions interact with specific ECM proteins and sense the rigidity of the substrate to trigger signalli...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1111/boc.201700060
更新日期：2018-03-01 00:00:00
abstract：:The entry into, and exit from, mitosis are controlled by a universal M-phase promoting factor (MPF) composed of at least p34cdc2 and a cyclin. Embryonic systems are convenient for studying the association and dissociation of the active MPF complex because oocytes and eggs are naturally arrested at a specific point of ...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/0248-4900(92)90137-p
更新日期：1992-01-01 00:00:00
abstract：:PD (peritoneal dialysis) is an established mode of renal replacement therapy, based on the exchange of fluid and solutes between blood in peritoneal capillaries and a dialysate that has been introduced into the peritoneal cavity. The dialysis process involves diffusive and convective transports and osmosis through the...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1042/BC20040132
更新日期：2005-09-01 00:00:00
abstract：BACKGROUND INFORMATION:Loss of sensitivity to TGF-beta1 (transforming growth factor beta1)-induced growth arrest is an important step towards malignant transformation in human epithelial cells, and Id-1 (inhibitor of differentiation or DNA binding-1) has been associated with cell proliferation and cell-cycle progressio...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20060026
更新日期：2006-09-01 00:00:00
abstract：BACKGROUND INFORMATION:CLEM (correlative live cell and electron microscopy) seeks to bridge the data acquired with different imaging strategies, typically between light microscopy and electron microscopy. It has been successfully applied in cell cultures, although its use in multicellular systems is hampered by difficu...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20090096
更新日期：2009-12-04 00:00:00
abstract：:Circulating glucocorticoid (GC) levels are thought to modulate the basal activity of pro-opiomelanocortin (POMC) neurons within the mediobasal hypothalamus (MBH) of the male rat. In a recent study we demonstrated that Fos-immunoreactivity (Fos-IR) was spontaneously induced throughout the dark phase of the light/dark c...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1997-12-01 00:00:00
abstract：:Hepatitis C virus (HCV) encodes two envelope glycoproteins, E1 and E2, that are released from HCV polyprotein by signal peptidase cleavage. These proteins assemble as a noncovalent heterodimer that is retained in the endoplasmic reticulum. The transmembrane domains of E1 and E2 are multifunctional and play a major rol...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/j.biolcel.2004.03.008
更新日期：2004-08-01 00:00:00
abstract：:Vertebrate embryos are able to reconstitute the body plan when early blastomeres are deleted, but it is not known whether this is accomplished by cells local to the lesion or by a readjustment of the entire pattern of the embryo. We distinguished between these two possibilities by studying which embryonic cells change...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/j.biolcel.2004.06.002
更新日期：2004-09-01 00:00:00
abstract：BACKGROUND INFORMATION:The assembly and maintenance of cilia depend on IFT (intraflagellar transport) mediated by molecular motors and their interplay with IFT proteins. Here, we have analysed the involvement of IFT proteins in the ciliogenesis of mammalian photoreceptor cilia. RESULTS:Electron microscopy revealed tha...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20110034
更新日期：2011-10-01 00:00:00
abstract：:We studied mechanisms that mediate recognition of human erythrocytes (HRBC) and sheep erythrocytes (SRBC) by rat liver macrophages. We used an in vitro cell binding assay that allows spontaneous formation of cell contacts. Binding of HRBC to rat macrophages shows the following characteristics: inhibition studies with ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00558.x
更新日期：1987-01-01 00:00:00
abstract：:An interaction between human poly(A)-binding protein (PABP) et human eRF3 has been demonstrated using a double-hybrid approach and in vitro assays. Here, we show that the binding of both proteins is conserved through evolution. We also demonstrate that the last 39 C-terminal amino acids of PABP contain the interface t...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(02)01194-2
更新日期：2002-09-01 00:00:00
abstract：:Primary cultures of cells from breast carcinomas were attempted in 74 cases. Growth was observed in 46 cases. Using immunochemical demonstration of keratin proteins (KER), epithelial membrane antigen (EMA) and carcinoembryonic antigen (CEA), three morphologically distinct cell populations were characterized and descri...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00574.x
更新日期：1987-01-01 00:00:00
abstract：:The first step of polyamine uptake is the binding of polyamines to the cell membrane. In order to characterize the specificity of the putrescine binding sites at the surface of the glioblastoma cells (U251), we have carried out competition experiments between putrescine bound to latex microspheres and vizualized by sc...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90369-e
更新日期：1990-01-01 00:00:00
abstract：:A central issue in the understanding of Marfan syndrome deals with the functional architecture of fibrillin-containing microfibrils. Fibrillin-rich microfibrils are long extracellular matrix fibrillar components exhibiting a 50 nm periodic beaded-structure with a width of around 20-25 nm after rotary shadowing and a 1...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/s0248-4900(98)80018-x
更新日期：1998-06-01 00:00:00
abstract：:Detection of nucleic acid sequence at the ultrastructural level has allowed us to better understand the expression of genes in some fields of application in cell biology. In situ hybridization at the ultrastructural level can be carried out using three different methods: on vibratome sections before embedding in epoxy...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：
更新日期：1998-07-01 00:00:00
abstract：:Flow DNA analysis was performed on samples from 71 surgically removed lung cancers and from 145 patients undergoing bronchoscopy. Abnormal DNA stem lines, characterized by their DNA index (DI), were frequently observed in operated lung carcinomas (87%). Two or three abnormal DNA stem lines were discovered simultaneous...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1986.tb00494.x
更新日期：1986-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Available data concerning the sperm morphology of teleost fishes demonstrate wide variation. In the present study, the spermatozoa of Siberian sturgeon (Acipenser baerii Brandt, 1869), a chondrostean fish, was investigated. In contrast with teleost fish, chondrostean spermatozoa have a head with ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20060060
更新日期：2007-02-01 00:00:00
abstract：BACKGROUND:The translocase of the mitochondrial inner membrane (TIM) imports most of the nucleus-encoded proteins that are destined for the matrix, inner membrane (IM) and the intermembrane space (IMS). Trypanosoma brucei, the infectious agent for African trypanosomiasis, possesses a unique TIM complex consisting of se...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/boc.202000054
更新日期：2020-10-20 00:00:00
abstract：:The clonal rat rhabdomyosarcoma cell line BA-HAN-1C is composed of proliferating mononuclear cells, some of which spontaneously fuse to terminally differentiated myotube-like giant cells. This cell line has been shown to be susceptible to differentiation induction with all-trans retinoic acid (RA). Since it is still u...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(94)90052-3
更新日期：1994-01-01 00:00:00
abstract：:A digitally-enhanced videomicroscopy study of rabbit gastric parietal cells in primary culture was performed using alternate observations with differential interference contrast and fluorescence optics of cells mounted and perfused on a temperature-controlled microscope stage. The effect of histamine, a physiological ...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(90)90349-8
更新日期：1990-01-01 00:00:00
abstract：:Flow cytometry (FCM) analysis of cellular DNA content has become an increasingly important clinical research tool for the measurement and identification of abnormal cell populations. Examination of cellular DNA content can provide information for use in cell cycle analysis in cancer studies. Most researchers would agr...
journal_title：Biology of the cell
pub_type： 杂志文章,评审
doi：10.1016/0248-4900(93)90116-v
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND INFORMATION:Proliferating cell nuclear antigen (PCNA) is a key component of the DNA replication machinery involved in the process of DNA elongation, recombination, methylation and repair. We have used PCNA fused with green fluorescent protein (GFP-PCNA) as a convenient tool to show the progress of S-phase in...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1042/BC20040093
更新日期：2005-03-01 00:00:00
abstract：:Cell nuclei may contain significant quantities of the metals Fe, Ni, Cu, Zn, since they are present in the nucleo-enzymes and/or nucleic acids. These metals have been detected by X-ray microanalysis in situ in dinoflagellates (Kearns et al). Aluminum was only detected in cell nuclei in cases of natural or provoked int...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1111/j.1768-322x.1987.tb00577.x
更新日期：1987-01-01 00:00:00
abstract：:Bovine tracheal gland (BTG) cells in culture show an epithelial-fibroblastoid transition after several passages. To investigate these BTG cell phenotype changes, we studied the effects of both the culture medium and passage number on the expression of epithelial cytoskeletal proteins and glandular serous cell markers....
journal_title：Biology of the cell
pub_type： 杂志文章
doi：10.1016/0248-4900(91)90008-b
更新日期：1991-01-01 00:00:00
abstract：:Detergent permeabilized Euplotes eurystomus (a fresh water hypotrichous ciliate) was reacted with monoclonal and polyclonal antibodies specific for either detyrosinated or tyrosinated alpha-tubulin (Glu- or Tyr-tubulin). The isolated cytoskeleton-nuclear complex was examined by Western immunoblotting and by immunofluo...
journal_title：Biology of the cell
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
